Meeting the buffer requirements of concentrate-fed dairy cows is a challenging task. We evaluated the effect of supplementing different feed additives to diets containing K2CO3 on feed intake, nutrient digestion and milk production of dairy cows. Eight peak lactation high yielding Holstein cows were used in a replicated 4 × 4 Latin square design with 4 periods of 21 d (14 d for diet adaptation and 7 d for sampling and data collection). Treatments comprised control (a diet containing a rumen buffer with the active component of K2CO3 at 6% of dietary dry matter (DDM) and containing 10% K); yeast, the control diet supplemented with live Saccharomyces cerevisiae yeast (0.06% of DDM); essential oils (EO), the control diet supplemented with essential oils (0.02% of DDM) and; peptides, the control diet supplemented with biogenic peptides (0.16% of DDM). Feed intake was greater (P < 0.001) in the yeast treatment (33.2 kg/d) compared to the others (30.9 to 32.1 kg/d). Dry matter and starch digestibilities were similar across the treatments. Cows that were supplemented with EO had a greater energy-corrected milk yield than control, and those supplemented with yeast and peptides were intermediate. Milk protein production was greater (P < 0.01) in EO and peptide treatments than in the control, with yeast treatment intermediate. In high-concentrate diets containing K2CO3, supplementing with yeast increased feed intake while supplementing wth essential oils increased energy-corrected milk yield, with small effects on milk protein yield.

In isolated subclavian artery, abnormal aortic arch development causes a loss of continuity with the aorta. Patent ductus arteriosus is a known cause of congestive heart failure. Herein, we present a rare case of congestive heart failure caused by isolated right subclavian artery and right patent ductus arteriosus associated with left-sided aortic arch treated by early closure.

This Research Communication describes the frequency of occurrence of P. fluorescens, P. putida and P. aeruginosa in raw goat milk stored at 4 and 9°C, to evaluate the spoilage potential and capacity of these species and the presence of the aprX gene, responsible for the synthesis of metalloprotease aprX. Samples of raw goat milk stored at 4 and 9°C for 72 h were plated on Pseudomonas CFC agar base (25°C for 48 h). The presence of P. fluorescens, P. putida, P. aeruginosa and aprX gene were confirmed by polymerase chain reaction. The isolates were evaluated for their proteolytic and lipolytic spoilage capacity and potential using milk agar (10%) and tributyrin agar base (1%), respectively (21°C for 72 h). A total of 184 strains of Pseudomonas spp. were obtained. At both temperatures, P. fluorescens was the most frequent, mostly proteolytic, and the only one to express high proteolytic and lipolytic potentials. Regardless of the temperature, all isolated strains of P. putida were lipoproteolytic, with a predominance of protease synthesis. Strains of P. aeruginosa (4,3%) were isolated occasionally (4.3% of total isolates) but only at 4°C, and 75% of these were lipoproteolytic. The storage temperature of raw goat milk influenced the frequency of P. aeruginosa, but not its spoilage potential. The presence of the aprX gene was verified in most strains isolated from all species at the two temperatures evaluated, although some of these strains did not express proteolytic capacity. Most isolates of Pseudomonas spp. showed lipoproteolytic capacity, which emphasizes the importance of ensuring low initial populations of this genus in raw chilled goat milk when it is used to produce dairy products.

According to the model of exchange as mutual assistance, an exchange can be perceived as a joint activity for mutual benefit – and needn’t involve any self-directed motives at all. This essay pushes back against this new defence of market motives. The essay develops an alternative ideal of production as caring solidarity, in which production is a joint activity of caring about one another. Points of overlap and difference are developed in some detail. The essay concludes by discussing the implications for an economics of caring solidarity, with discussion of the limitations of various market socialist strategies.

Political science was once silent about—and for many decades continued to be slow to address—LGBTQ+ politics as a topic worthy of scholarly research. One of the longest-standing gaps in the literature has been the lack of work that was pioneered by Ken Sherrill: research that centers LGBTQ+ people and politics as subjects, rather than objects, of study. Here I make the case for sustained scholarly attention to LGBTQ+ political behavior and discuss how quantitative empirical research in this vein is more feasible than ever before. I then provide an example of what is possible today with analyses of the 2022 Cooperative Election Study (CES), a large representative sample survey (complete case N = 45,240; LGBTQ+ N = 5,213) that includes questions about respondents’ sexual and gender identities. The analyses reveal several discoveries about LGBTQ+ people’s political behavior and lived experiences, including that they are no more politically engaged than the typical American, are in much poorer health than any other group, and belying stereotypes, are not of higher socioeconomic status than other Americans. A spatial representation of groups’ positions on the US political landscape shows that LGBTQ+ people are relatively distant from other groups, indicating that they may struggle to find natural coalition partners because of lack of shared interests.

Socialist Yugoslavia and Zambia became dynamic Cold War partners in the Non-Aligned Movement, with extensive cooperation in economic development, national defence, and international diplomacy. This article explores the roots of this “East–South” cooperation by looking at the pioneering contacts between North Rhodesian and Yugoslav trade unions in the late 1950s and early 1960s, showing how Yugoslav trade union officials opened up new perspectives for the Yugoslav organized labour movement as it reached out to the “global” at a time of rising decolonization and incipient non-alignment. Further, it offers a nuanced perspective on Cold War trade union internationalism and sheds new light on the politicization of the Zambian labour movement. The article shows that the national trade union federations and their officials on both sides were proactive sociopolitical actors, paving the way for future diplomatic contacts akin to “workers’ proto-diplomacy”.

This paper reports the methods and preliminary findings of Germina, an ongoing cohort study to identify biomarkers and trajectories of executive functions and language development in the first 3 years of life. 557 mother-infant dyads (mean age of mothers 33.7 years, 65.2% white, 48.7% male infants) have undergone baseline and are currently collecting data for other timepoints. A linear regression was used to predict baseline Bayley-III using scores derived from data-driven sparse partial least squares utilizing a multiple holdout framework of 15 domains. Significant associations were found between socioeconomic/demographic characteristics (B = 0.29), epigenetics (B = 0.11), EEG theta (B = 0.14) and beta activity (B = 0.11), and microbiome functional pathways (B = 0.08) domains, and infant development measured by the Bayley-III at T1, suggesting potential interventions to prevent impairments.

Crises constitute a fascinating context in which to investigate the resilience of institutional arrangements, or their breakdown and change, and to shed light on the interplay between formal and informal institutions in this process. The papers in this symposium focus on crises from political power grab to economic shock and natural disasters. They focus on the differing impact of different crises or investigate the specific impact of one form of crises on formal and informal institutions or the negotiation process that allow them to coexist. Bringing them under one roof emphasises the diversity of lenses through which institutions can be conceptualised and operationalised. It also highlights some of the issues preventing meaningful comparisons across frameworks. Importantly, it also allows us to trace an agenda for research towards improving our understanding of when and how crises lead to change. We argue that an often under-studied aspect that could help to move towards a clearer taxonomy is to articulate more explicitly the agency of actors and the distribution of power within society and social groups.

We prove that a C$^*$-algebra A has uniform property $\Gamma $ if the set of extremal tracial states, $\partial _e T(A)$, is a non-empty compact space of finite covering dimension and for each $\tau \in \partial _e T(A)$, the von Neumann algebra $\pi _\tau (A)"$ arising from the GNS representation has property $\Gamma $.

Milk fat synthesis is tightly regulated by hormones and growth factors. Leptin is a versatile peptide hormone that exerts pleiotropic effects on metabolic pathways. In this study, we evaluated the expression and function of leptin and its long form receptor OB-Rb in dairy cow mammary tissues from different physiological stages and in cultured mammary epithelial cells. The results showed that the expression of leptin and OB-Rb were significantly higher in the mammary tissues of lactating cows as compared with dry cows, suggesting that they are related to milk component synthesis. In cultured dairy cow mammary epithelial cells, leptin treatment significantly increased OB-Rb expression and intracellular triacylglycerol content. Transcriptome analysis identified the difference in gene expression between leptin treated cells and control cells, and 317 differentially expressed genes were identified. Gene ontology and pathway mapping showed that lipid metabolism-related gene expression increased and signal transduction pathway-related genes were the most significantly enriched. Mechanistic studies showed that leptin stimulation enhanced sterol regulatory element-binding protein 1 expression via activating the phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin (PI3K/AKT/mTOR) signaling pathway, which in turn up-regulated the expression of genes related to milk fat synthesis. Moreover, we found that fatty acid synthesis precursors, acetate and β-hydroxybutyrate, could positively regulate the expression of leptin and OB-Rb in bovine mammary epithelial cells, thereby potentially increasing milk fat synthesis. Our study provided novel evidence in the regulation of leptin on milk fat production in mammary glands of dairy cows, as well as experimental basis for artificial regulation of milk fat

The ghost shrimp Audacallichirus mirim had been previously recorded along the Brazilian coast. However, this study marks its first report in southern Espírito Santo and northern Rio de Janeiro, in southeastern Brazil. Two male specimens were collected at Piúma Beach (Espírito Santo) and Guaxindiba Beach (Rio de Janeiro) in March 2023, using a steel suction pump in the intertidal zone. The individuals were identified in a laboratory based on taxonomic characteristics. Abiotic factors such as salinity, temperature, and dissolved oxygen were measured at the collection sites, revealing distinct environmental conditions between the two beaches. Granulometric analysis showed significant differences in sediment composition, with Piúma characterized by finer sands and Guaxindiba by a more poorly sorted sediment, with a higher contribution of coarser grains. This record contributes to understanding the biogeographical distribution of A. mirim, emphasizes its ecological role, and highlights the need for further research on its population dynamics and habitat preferences in southeastern Brazil.