The impact of vitamin D supplementation on depressive symptoms remains uncertain. This study aimed to investigate the dose-dependent effects of vitamin D supplementation on depressive and anxiety symptoms in adults. We systematically searched PubMed, Scopus, and Web of Science up to December 2022 to identify randomized controlled trials evaluating the effects of vitamin D3 supplementation on depression and anxiety symptoms in adults. Using a random-effects model, we calculated the standardized mean difference (SMD) for each 1000 IU/day vitamin D3 supplementation. The GRADE tool assessed the certainty of evidence. Our analysis included 31 trials with 24189 participants. Each 1000 IU/day vitamin D3 supplementation slightly reduced depressive symptoms in individuals with and without depression (SMD: −0.32, 95% CI −0.43 to −0.22; GEADE = moderate). The effect was more pronounced in those with depressive symptoms (SMD: −0.57, 95% CI −0.69 to −0.44; n = 15). The greatest reduction occurred at 8000 IU/day (SMD: −2.04, 95% CI −3.77 to −0.31). Trials with follow-up ⩽8 weeks (SMD: −0.45, 95% CI −0.70 to −0.20; n = 8) and 8 to ⩽24 weeks (SMD: −0.47, 95% CI −0.70 to −0.24; n = 15) showed stronger effects compared to those lasting 24 to ⩽52 weeks (SMD: −0.13, 95% CI −0.28 to 0.02; n = 5) or longer than 52 weeks (SMD: 0.14, 95% CI −0.16 to 0.44; n = 3) (p group difference <0.001). Vitamin D3 supplementation had no significant effects on anxiety symptoms. In summary, this study suggests that vitamin D3 supplementation may effectively reduce depressive symptoms in short term. Further high-quality trials are warranted for a conclusive assessment of its impact on anxiety.

Khakiweed is a perennial broadleaf weed that is difficult to control because of its multiple means of reproduction, vigorous growth, and deep taproot. Khakiweed reduces the performance of pasture, pecan, and turf areas by choking out desirable grass and legume species. Because information on the effectiveness of contact and residual herbicides for control in pecan and pasture areas is limited, greenhouse studies were conducted to determine the effect of application timing, mode of action, and rate on khakiweed control. Preemergence and postemergence herbicides were applied to mature khakiweed plants at 0.25X, 0.5X, 1X, or 2X the label recommended rate for general broadleaf control. Biomass was collected 3 wk after application. Plants regrew from roots in the greenhouse until a second biomass harvest was collected at 6 wk after treatment (WAT). Metsulfuron-methyl, indaziflam, or pendimethalin was applied preemergence to the soil surface. All rates of preemergence herbicides provided high-efficacy control of regrowth (>85%) compared to the nontreated control. The efficacies of postemergence-applied metsulfuron-methyl, metsulfuron-methyl + nicosulfuron, indaziflam, aminopyralid + florpyrauxifen-benzyl, 2,4-D amine, and 2,4-D amine + florpyrauxifen-benzyl were also examined. All postemergence herbicide treatments exhibited control compared to the nontreated plants at both sample timings (3 and 6 WAT) and increased with herbicide application rate. No herbicide provided high-efficacy control during the initial postspray period (0 to 3 WAT). During the regrowth period (3 to 6 WAT), metsulfuron-methyl alone and in combination gave >85% control of khakiweed biomass, indicating that the sulfonylurea herbicides used in this study are well suited to controlling khakiweed.

Bearded sprangletop is a problematic native grass weed in California’s rice fields. The widespread and extensive use of acetyl-CoA carboxylase (ACCase)–inhibiting herbicides, such as cyhalofop-p-butyl (cyhalofop), has led to speculation that biotypes of bearded sprangletop have developed herbicide resistance to ACCase. The aim of this study was to evaluate suspected resistant bearded sprangletop biotypes, R1, R2, R3, and the susceptible biotype, S1, in terms of their levels of resistance to three ACCase-inhibiting herbicides and to characterize the molecular mechanisms of resistance. Dose–response experiments suggested that the biotype R1, R2, and R3 had high-level resistance to cyhalofop and to quizalofop-p-ethyl (quizalofop), but not clethodim. The study determined that the resistance to ACCase inhibitors was a target-site mechanism resulting from nucleotide substitution. The carboxyl transferase (CT) domain of the ACCase gene’s sequence analysis revealed the substitutions Trp-2027-Cys for R1 and R2 biotypes and Ile-2041-Asn for the R3 biotype. This study revealed the presence of target-site resistance to cyhalofop and quizalofop in at least two mutation points in representative biotypes of bearded sprangletop in California. This research highlights the significance of careful herbicide selection due to weed species responding quite rapidly to selection pressure, so as to aid in managing bearded sprangletop in rice fields.

We aimed to investigate the effectiveness of n-3 fatty acids supplementation on the risk of developing depression, depressive symptoms and remission of depression. We searched PubMed, Scopus and Web of Science from inception to December 2022 to find randomised trials of n-3 fatty acids supplementation in adults. We conducted random-effects meta-analyses to estimate standardised mean differences (SMD) and 95 % CI for continuous outcomes and risk difference and 95 % CI for binary outcomes. A total of sixty-seven trials were included. Each 1 g/d n-3 fatty acids supplementation significantly improved depressive symptoms in adults with and without depression (moderate-certainty evidence), with a larger improvement in patients with existing depression. Dose–response analyses indicated a U-shaped effect in patients with existing depression, with the greatest improvement at 1·5 g/d. The analysis showed that n-3 fatty acid supplementation significantly increased depression remission by 19 more per 100 in patients with depression (low-certainty evidence). Supplementation with n-3 fatty acids did not reduce the risk of developing depression among the general population, but it did improve the severity of depression among patients with existing depression.

Indaziflam and oxadiazon are efficacious preemergence herbicides used in warm-season turfgrass because of their persistence and residual activity. It is beneficial to quantify effective concentrations for preemergence control of summer annual weeds and determine whether these concentrations are maintained throughout weed emergence periods. Therefore, greenhouse bioassays were conducted with barnyardgrass, broadleaf signalgrass, doveweed, large crabgrass, and purple nutsedge. Treatments included indaziflam at 0, 4, 8, 12, 17, 21, 25, 29, 33, and 37 g ai ha−1 or oxadiazon at 0, 420, 841, 1,260, 1,681, 2,102, 2,354, 2,942, 3,363, and 3,783 g ha−1. Although preemergence herbicides are not used to control perennial weeds, purple nutsedge was included to investigate the effect of selected herbicides on its growth. Herbicide EC50, EC80, and EC90 for seedling emergence inhibition and shoot and root mass reduction were quantified from log-logistic dose–response curves. Herbicide concentration that remains from a preemergence application during the regional species-specific periodicity of emergence was predicted using first-order kinetics equations. Indaziflam and oxadiazon controlled seedling emergence 14 d after treatment (DAT) in the evaluated annual weeds and shoot and root mass in all species 84 DAT. Indaziflam applied in mid-March at 33 g ha−1 may provide up to 90% seedling emergence inhibition in large crabgrass and signalgrass; up to 80% in barnyardgrass; and up to 50% in doveweed. Oxadiazon applied in mid-March at 3,363 g ha−1 may provide up to 80% seedling emergence inhibition in all species. Indaziflam and oxadiazon may control up to 80% shoot mass and up to 50% root mass, respectively, in purple nutsedge and 80% to 90% shoot or root mass in other species. Such information is useful in evaluating adequacy of herbicide management practices for season-long weed control, and it aids turfgrass managers in applying preemergence herbicides at optimal timing based on target weed species.

We performed a systematic review and dose–response meta-analysis of randomised trials on the effects of olive oil consumption on blood lipids in adults. A systematic search was performed in PubMed, Scopus and Web of Science databases until May 2021. Randomised controlled trials (RCT) evaluating the effect of olive oil intake on serum total cholesterol (TC), TAG, LDL-cholesterol and HDL-cholesterol in adults were included. The mean difference (MD) and 95 % CI were calculated for each 10 g/d increment in olive oil intake using a random-effects model. A total of thirty-four RCT with 1730 participants were included. Each 10 g/d increase in olive oil consumption had minimal effects on blood lipids including TC (MD: 0·79 mg/dl; 95 % CI (−0·08, 1·66); I2 = 57 %; n 31, GRADE = low certainty), LDL-cholesterol (MD: 0·04 mg/dl, 95 % CI (−1·01, 0·94); I2 = 80 %; n 31, GRADE = very low certainty), HDL-cholesterol (MD: 0·22 mg/dl; 95 % CI (−0·01, 0·45); I2 = 38 %; n 33, GRADE = low certainty) and TAG (MD: 0·39 mg/dl; 95 % CI (−0·33, 1·11); I2 = 7 %; n 32, GRADE = low certainty). Levels of TC increased slightly with the increase in olive oil consumption up to 30 g/d (MD30 g/d: 2·76 mg/dl, 95 % CI (0·01, 5·51)) and then appeared to plateau with a slight downward curve. A trivial non-linear dose-dependent increment was seen for HDL-cholesterol, with the greatest increment at 20 g/d (MD20 g/d: 1·03 mg/dl, 95 % CI (−1·23, 3·29)). Based on existing evidence, olive oil consumption had trivial effects on levels of serum lipids in adults. More large-scale randomized trials are needed to present more reliable results.

Triclopyr is a synthetic auxin herbicide currently available as a triethylamine salt, butoxyethyl ester, pyridinyloxyacetic acid, or choline salt. The formulation of a herbicide has the potential to impact its activity; therefore, the objective of this study was to determine the relative activity of these four triclopyr formulations. Greenhouse dose–response studies were conducted twice at the University of Florida in 2015. The four formulations were foliar applied at rates ranging from 17 to 1,121 g ae ha−1 to 2- to 3-leaf soybean, sunflower, tomato, and cotton. The amine salt formulation provided the lowest ED50 values in tomato and sunflower (22.87 and 60.39 g ha−1, respectively); whereas in soybean, amine and choline formulations provided the lowest ED50 values (22.56 and 20.95 g ha−1, respectively). No differences between formulations were observed in cotton. These data suggest that (1) the amine salt formulation of triclopyr might be more active than the others on tomato and sunflower, and (2) the amine and choline salt formulations might be more active than the others on soybean. Further work must be conducted to determine whether there are differences among these formulations under a range of field conditions and target species. In addition, other important management factors such as applicator safety, volatility potential, and cost should be considered when choosing the best formulated product to be applied.

A 2,4-D-resistant tall waterhemp population (FS) from Nebraska was evaluated for resistance to other TIR1 auxin receptor herbicides and to herbicides having alternative mechanisms of action using greenhouse bioassays and genetic markers. Atrazine, imazethapyr, lactofen, mesotrione, glufosinate, and glyphosate were applied in a single-dose bioassay, and tissue was collected from marked plants for genetic analysis. The FS population was not injured by atrazine or by imazethapyr. Approximately 50% of the plants survived lactofen and were actively growing 28 d after treatment. The population was susceptible to mesotrione, glufosinate, and glyphosate. Ametryn, chlorimuron-ethyl, 2,4-D, aminocyclopyraclor, aminopyralid, and picloram were applied in dose–response studies. The FS population was sensitive to ametryn, and the Ser-264-Gly substitution in the D1 protein was not detected, suggesting the lack of response to atrazine is not due to a target-site mutation. The FS population exhibited less than 50% injury to chlorimuron-ethyl at application rates 20 times the labeled use rate. The Ser-653-Asn acetolactate synthase (ALS) substitution, which confers resistance to imidazolinone herbicides, was present in the FS population. However, this does not explain the lack of response to the sulfonylurea herbicide, chlorimuron-ethyl. Sequencing of a portion of the PPX2L gene did not show the ΔG210 mutation that confers resistance to protoporphyrinogen oxidase–inhibiting herbicides, suggesting that other factors were responsible for waterhemp survival after lactofen application. The FS population was confirmed to be at least 30-fold resistant to 2,4-D relative to the susceptible populations. In addition, it was at least 3-fold less sensitive to aminopyralid and picloram, two other TIR1 auxin receptor herbicides, than the 2,4-D-susceptible populations were. These data indicated that the FS population contains both target and non–target site mechanisms conferring resistance to herbicides spanning at least three mechanisms of action: TIR1 auxin receptors, ALS inhibitors, and photosystem II inhibitors.

Adoption of soybean that is resistant to 2,4-D will result in more use of glyphosate plus 2,4-D premixes and tank mixtures. Preliminary whole-plant greenhouse assays confirm most Palmer amaranth populations found in Indiana are glyphosate-resistant (GR), and some biotypes exhibit tolerance to 2,4-D amine. Dose–response experiments were conducted to determine the level of glyphosate resistance and 2,4-D amine tolerance in four Palmer amaranth biotypes. A premix formulation of glyphosate plus 2,4-D choline was also evaluated. The R1, R2, and R3 biotypes were 31- to 66-fold more resistant to glyphosate (R:S ratio) than the S1 biotype based on the herbicide dose to cause 90% mortality (LD90). The maximum POST rate of the premix formulation of Enlist Duo® labeled in ‘Enlist®’ soybean is 2,195 g ae ha−1. When separated by active ingredient, the maximum POST rate of Enlist Duo® is equivalent to 1,141 and 1,054 g ae ha−1 of glyphosate and 2,4-D choline, respectively. In the absence of glyphosate, the maximum rate of 2,4-D (1,054 g ae ha−1) in the premix formulation of Enlist Duo® controlled S1, R2, and R3 biotypes, but failed to control all plants from the R1 biotype. Estimates for LD90 showed the R1 biotype was 3-fold more tolerant than the S1 biotype to 2,4-D amine. However, no plants survived the 1,155 g ae ha−1 (600 g ae ha−1 of glyphosate plus 555 g ae ha−1 2,4-D) treatment with the premix formulation of glyphosate plus 2,4-D choline. Overall, results from this experiment suggest GR Palmer amaranth that also exhibits increased tolerance to 2,4-D amine will be difficult to control with glyphosate or 2,4-D alone, but can be controlled POST with Enlist Duo® at lower than labeled field rates (1,618 to 2,195 g ae ha−1).

There are an estimated 400 million hectares of non-cropland in the United States primarily designated as rangeland and pastureland, and there are more than 300 invasive weeds found on these sites, causing an estimated annual loss of $5 billion. Among the most invasive and problematic weeds are Dalmatian toadflax, diffuse knapweed, downy brome, and musk thistle. Currently, herbicides are the most common management strategy for broadleaf weeds and invasive winter annual grasses. Indaziflam, a new herbicide for invasive plant management in non-crop areas, is a cellulose-biosynthesis inhibitor capable of providing residual invasive winter annual grass control up to 3 yr after treatment (YAT). A field experiment was conducted to determine whether residual Dalmatian toadflax and downy brome control by aminocyclopyrachlor, imazapic, and picloram could be extended by tank mixing these herbicides with indaziflam. Indaziflam tank mixed with aminocyclopyrachlor, imazapic, and picloram provided increased Dalmatian toadflax (84% to 91%) and downy brome (89% to 94%) control 4 YAT, compared with treatments excluding indaziflam. Treatments without indaziflam controlled 50% to 68% of Dalmatian toadflax and <25% downy brome 4 YAT. Based on these results, a greenhouse dose–response experiment was conducted with aminocyclopyrachlor, aminopyralid, and indaziflam to compare preemergence control of nine common non-crop weeds. Averaged across species, aminocyclopyrachlor and aminopyralid GR50 values (herbicide concentration resulting in 50% reduction in plant biomass) were 29 and 52 times higher compared with indaziflam, respectively. These data suggest that indaziflam could be used for residual control of non-crop weeds as a tank-mix partner with other foliar-applied broadleaf herbicides.

Yellow nutsedge infests a large number of hectares in the Treasure Valley of eastern Oregon and western Idaho. Much of its continued expansion appears to be related to onion production in the valley. Fall applications of metham often produce inconsistent results when used to control yellow nutsedge before planting an onion crop. Trials were conducted in the laboratory to determine the influence on yellow nutsedge control of metham dose, duration of exposure, temperature during exposure, and tuber conditioning by washing and chilling at 3 C. All factors influenced metham efficacy against yellow nutsedge tubers. The dose causing 50% reduction in sprouting tubers (I 50) for metham ranged from 22 to 76 mg kg−1 of soil and was lower for conditioned tubers than nonconditioned tubers across all conditions, except when tubers were exposed at 25 C for 3 d. Nonconditioned tubers were unaffected by metham after 1 d exposure at 5 C. Increasing exposure temperature or increasing exposure duration decreased sprouting for nonconditioned tubers. As exposure duration and exposure temperature increased, differences among conditioned and nonconditioned tubers were less. Temperature and exposure duration affects metham efficacy against yellow nutsedge, and the condition of the tubers at the time of treatment also has a significant effect. Applications of metham at a time when yellow nutsedge tubers are not dormant may improve yellow nutsedge control.

A study was conducted to determine the dose–response of purple nutsedge tuber sprouting and plant growth to glyphosate. Tuber sprouting on day 6 satisfactorily fitted the probit model, with a predicted effective dosage causing 50% inhibition of response (ED50) of 30 mM, which is similar to that with 25 mM of ED50 on day 3. In addition, together with the dose–response analysis of bud growth on day 6, it was shown that bud growth of purple nutsedge was more sensitive to root- or tuber-absorbed glyphosate or both than was tuber sprouting; the former had 1.25 mM of ED50. During the V1–2 stage, purple nutsedge injury, including shoot dehydration, wilting, and yellowing, appeared sequentially when glyphosate was absorbed. When compared with the dose–response of shoot greenness of purple nutsedge at the V5–7 stage under the root-absorbed glyphosate treatment, purple nutsedge at the V1–2 stage was less sensitive to root-applied glyphosate. Further studies for determining the efficacy of glyphosate applied on root and foliage of purple nutsedge at the V5–7 stage showed that injury from glyphosate occurred within 7 d after foliar treatment, and the ED50 values of glyphosate for shoot survival and leaf chlorophyll were 13.1 and 14.5 mM, respectively. However, the response of plants was less sensitive to root-absorbed glyphosate, which had an ED50 of 101 mM. This finding might be the result of direct injury of roots caused by glyphosate, resulting in the delayed and diluted effect of this herbicide from root to shoot.

Palmer amaranth resistance to acetolactate synthase (ALS)–inhibiting herbicides was first identified in Georgia in 2000. Since then, complaints from peanut producers have increased concerning failure of ALS herbicides in controlling Palmer amaranth. Because efficacy of ALS herbicides can be compromised under adverse conditions, seeds from Palmer amaranth plants that escaped weed control were collected across the peanut-growing region in Georgia to investigate the cause of these reported failures. Greenhouse and growth-chamber studies were conducted using these seeds to evaluate whether weed escapes were a result of Palmer amaranth resistance to ALS herbicides. Each of the 61 accessions collected across Georgia exhibited varying levels of resistance to imazapic applied POST (< 55% control, relative to ALS-susceptible Palmer amaranth). Subsamples of the accessions were evaluated for their response to imazapic rates, which indicated variable levels of resistance across Palmer amaranth accessions. The rate of imazapic that provided 50% reduction in Palmer amaranth plant biomass (I50) for the known susceptible biotype was 0.9 g/ha of imazapic. Of the 10 accessions evaluated, 8 of them had I50 values that ranged from 3 to 297 g/ha of imazapic. The other two accessions could not be fit to the log-logistic dose–response curve and had undeterminable I50 values because of high levels of ALS resistance (> 1,400 g/ha of imazapic). Herbicide cross-resistance experiments indicated that 30 accessions were resistant to the ALS herbicides imazapic, chlorimuron, pyrithiobac, and diclosulam at the recommended field-use rates. However, each of these 30 accessions was susceptible to glyphosate. These data demonstrate that ALS-resistant Palmer amaranth occurs throughout the peanut-growing region of Georgia. Growers in Georgia will need to alter their weed-control programs in peanut to include herbicides with multiple modes of action that do not rely on ALS herbicides for effective Palmer amaranth control.