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Multiple-Herbicide Resistance in a 2,4-D–Resistant Waterhemp (Amaranthus tuberculatus) Population from Nebraska

Published online by Cambridge University Press:  05 September 2017

Roberto J. Crespo ,
Ana B. Wingeyer ,
Greg R. Kruger ,
Chance W. Riggins ,
Patrick J. Tranel  and
Mark L. Bernards
Roberto J. Crespo*
Affiliation:
Graduate Research Assistant and Graduate Student, Department of Agronomy and Horticulture, University of Nebraska–Lincoln, Lincoln, NE 68583-0915
Ana B. Wingeyer
Affiliation:
Researcher, Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Paraná, and CONICET, Oro Verde, Entre Ríos, E3101, Argentina
Greg R. Kruger
Affiliation:
Associate Professor, West Central Research and Extension Center, University of Nebraska–Lincoln, North Platte, NE 69101-7751
Chance W. Riggins
Affiliation:
Postdoc Research Associate and Professor, Department of Crop Sciences, University of Illinois, Urbana, IL 61801
Patrick J. Tranel
Affiliation:
Postdoc Research Associate and Professor, Department of Crop Sciences, University of Illinois, Urbana, IL 61801
Mark L. Bernards
Affiliation:
Assistant Professor, Department of Agronomy and Horticulture, University of Nebraska–Lincoln, Lincoln, NE 68583-0915
*
*Corresponding author’s E-mail: [email protected]
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Abstract

A 2,4-D-resistant tall waterhemp population (FS) from Nebraska was evaluated for resistance to other TIR1 auxin receptor herbicides and to herbicides having alternative mechanisms of action using greenhouse bioassays and genetic markers. Atrazine, imazethapyr, lactofen, mesotrione, glufosinate, and glyphosate were applied in a single-dose bioassay, and tissue was collected from marked plants for genetic analysis. The FS population was not injured by atrazine or by imazethapyr. Approximately 50% of the plants survived lactofen and were actively growing 28 d after treatment. The population was susceptible to mesotrione, glufosinate, and glyphosate. Ametryn, chlorimuron-ethyl, 2,4-D, aminocyclopyraclor, aminopyralid, and picloram were applied in dose–response studies. The FS population was sensitive to ametryn, and the Ser-264-Gly substitution in the D1 protein was not detected, suggesting the lack of response to atrazine is not due to a target-site mutation. The FS population exhibited less than 50% injury to chlorimuron-ethyl at application rates 20 times the labeled use rate. The Ser-653-Asn acetolactate synthase (ALS) substitution, which confers resistance to imidazolinone herbicides, was present in the FS population. However, this does not explain the lack of response to the sulfonylurea herbicide, chlorimuron-ethyl. Sequencing of a portion of the PPX2L gene did not show the ΔG210 mutation that confers resistance to protoporphyrinogen oxidase–inhibiting herbicides, suggesting that other factors were responsible for waterhemp survival after lactofen application. The FS population was confirmed to be at least 30-fold resistant to 2,4-D relative to the susceptible populations. In addition, it was at least 3-fold less sensitive to aminopyralid and picloram, two other TIR1 auxin receptor herbicides, than the 2,4-D-susceptible populations were. These data indicated that the FS population contains both target and non–target site mechanisms conferring resistance to herbicides spanning at least three mechanisms of action: TIR1 auxin receptors, ALS inhibitors, and photosystem II inhibitors.

Keywords

2,4-Dametrynaminocyclopyrachloraminopyralidatrazinechlorimuron-ethylglufosinateglyphosateimazethapyrlactofenmesotrionepicloramtall waterhemp, Amaranthus tuberculatus (Moq.) Sauer. AMATU.Cross-resistancedose–responseherbicide resistanceinjury
Type
Weed Biology and Ecology
Information
Weed Science , Volume 65 , Issue 6 , November 2017 , pp. 743 - 754
Copyright
© Weed Science Society of America, 2017 

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Footnotes

a

Current address of first author: Crop Consultant, Oro Verde, Entre Ríos, E3101, Argentina

b

Current address of sixth author: Associate Professor, School of Agriculture, Western Illinois University, Macomb, IL 61455.

Associate Editor for this paper: Franck E. Dayan, Colorado State University

References

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