Three resistant (R) rigid ryegrass (Lolium rigidum Gaudin) populations from southern Australia (EP162, 375-14, and 198-15) with cross-resistance to thiocarbamate, chloroacetamide, and sulfonylisoxazoline herbicides displayed reduced sensitivity to the isoxazolidinone herbicides bixlozone and clomazone. Each of these R populations was exposed to two cycles of recurrent selection (RS) in which plants were treated with the field rate of bixlozone, survivors were bulk crossed, and seed was collected. After the first cycle of recurrent selection (RS1), the LD50 to bixlozone in population 198-15 increased to 17.5-fold compared with the S population and increased further to 26.9-fold after a second cycle of recurrent selection (RS2). The recurrent selection process also increased the level of resistance to bixlozone in populations EP162 and 375-14 (7.8- to 18.4-fold) compared with the S population. Phorate antagonized bixlozone and clomazone in SLR4 (34.6- and 28.1-fold increase in LD50) and both herbicides in populations EP162 (36.5- to 46.6-fold), 375-14 (71.4- to 73.9-fold), and 198-15 (86.4- to 91.5-fold) compared with the absence of phorate. The increase in LD50 of all L. rigidum RS populations when treated with phorate suggests a lack of herbicide activation is not the likely resistance mechanism to these herbicides. This research highlights the elevated risk of thiocarbamate-resistant L. rigidum populations to rapidly evolve resistance to the isoxazolidinone herbicides bixlozone and clomazone.

Phorate {O,O-diethyl S-[(ethylthio)methyl] phosphorodithioate}, disulfoton {O,O-diethyl S-[2-(ethylthio)ethyl] phosphorodithioate}, linuron [3-(3,4-dichlorophenyl)-1-methoxy-1-methylurea], or metribuzin [4-amino-6-tert-butyl-3-(methylthio)-as-triazin-5(4H)-one] when used alone did not reduce soybean [Glycine max (L.) Merr. ‘Calland’] grain yield or plant population. However, combinations of metribuzin with either phorate or disulfoton interacted synergistically to reduce both soybean grain yield and plant population significantly even at recommended rates of both chemicals. Combinations of linuron with either phorate or disulfoton at recommended rates did not reduce soybean grain yield or plant population. Greenhouse studies further substantiated the synergistic interaction of phorate or disulfoton with metribuzin.

The concept of interaction arises in the context of continuous functions. Verbally, it is the action of one independent variable on the action of another, and vice versa, on a dependent variable. Quantitatively, it is the second partial derivative of a function with respect to two independent variables. Misconceptions have arisen from attempts to understand action and interaction in discrete terms. The algebraic expressions for the actions of diuron and phorate on the fresh weight of oats and their interaction were determined from the regression polynomial and plotted in three-dimensional graphs. Three other mutually incompatible methods of assessing interaction, namely, the Colby (with its modification, regression estimate), the two-parameter, and the topographical methods were evaluated.

Genetics can be a powerful adjunct to just about any kind of physiological study, including weed physiology or weed/herbicide interactions. Making, mapping, and reverting mutations is simple and straightforward. Making mutants can be as simple as isolating variant individuals from the “wild”, as uncomplicated as doing seed mutagenesis in your laboratory, or as sneaky as recovering mutants as sectors in whole plants. The overall principles for successful development of a protocol for seed mutagenesis of weeds are described and potential problem areas noted. These generalities are illustrated with a specific case history, that of chlorsulfuron. Although chlorsulfuron is accurately described as an inhibitor of the synthesis of branched chain amino acids, careful physiological examination suggests that it kills plant cells, not by starvation for amino acids, but by active toxicity of a metabolite, α-amino butyric acid, produced from a precursor available for diversion in cells with inhibited acetolactate synthase (EC 4.1.3.18, ALS). The story of dominant resistance due to an altered ALS enzyme is well known; analysis using additional mutants fleshes out the story of how chlorsulfuron works. Such analysis has the potential to help unravel other problems in weed physiology.

Field experiments were conducted to determine if aldicarb, disulfoton, or phorate applied in-furrow at 0.84 or 1.12 kg ai ha–1 affected cotton response to clomazone applied PPI or PRE at rates ranging from 0 to 1.12 kg ai ha–1. Disulfoton and phorate greatly reduced clomazone-induced chlorosis, stunting, and death of cotton seedlings. Compared with no insecticide or with dimethoate applied POST, aldicarb did not affect cotton response to clomazone. A clomazone rate by insecticide interaction was not observed for cotton yield. Clomazone reduced cotton yield at one of five locations. Insecticides affected yield at three locations, where highest yields were from cotton treated with aldicarb. In a separate experiment with 0.56, 1.12, and 2.24 kg ha–1 of phorate applied in-furrow and 1.12 kg ha–1 of clomazone applied PPI, phorate at all rates protected cotton from clomazone phytotoxicity.

Cotton tolerance of DPX-PE350 applied POST alone, mixed with acephate, carbaryl, or dimethoate applied at 220, 1100, or 280 g ai ha−1, respectively, or following aldicarb, disulfoton, or phorate applied in the seed furrow at 1.0, kg ai ha−1 was evaluated. DPX-PE350 at 170 g ae ha−1 did not adversely affect seed cotton yield, fiber length, fiber length uniformity, or micronaire but did decrease fiber strength. The insecticides did not alter cotton response to DPX-PE350. Entireleaf morningglory was controlled 92, 95, and 96% with DPX-PE350 applied POST at 56, 110, and 170 g ha−1, respectively. Mixing acephate, carbaryl, or dimethoate with DPX-PE350 did not affect control.

Field experiments were conducted in 1991 and 1992 to evaluate corn tolerance to nicosulfuron following in-furrow or surface-band applications of seven soil-applied insecticides. The organophosphate insecticides evaluated included terbufos (15G and 20CR formulations), phorate, chlorpyrifos, fonofos, and DPX-43898, as well as the synthetic pyrethroid tefluthrin. Potential for a nicosulfuron/ insecticide interaction to injure corn was ranked as follows: terbufos 15G = phorate > terbufos 20CR > fonofos. Chlorpyrifos, DPX-43898, or tefluthrin followed by nicosulfuron caused no significant corn injury. Corn injury and yield reduction were greater in corn treated in-furrow compared with surface-band. Soil moisture at nicosulfuron application was more influential in causing corn injury and loss of yield than growth stage of corn at nicosulfuron application.

Efficacy of herbicide programs containing clomazone PPI plus fluometuron PRE or clomazone plus pendimethalin PPI plus fluometuron PRE was compared with that of standard programs of pendimethalin PPI plus fluometuron PRE and norflurazon PPI plus norflurazon and fluometuron PRE. Cotton injury was less than 5% with all treatments when disulfoton or phorate was applied in the seed furrow. Control of fall panicum, goosegrass, large crabgrass, eclipta, entireleaf morningglory, ivyleaf morningglory, pitted morningglory, tall morningglory, prickly sida, redroot pigweed, smooth pigweed, hemp sesbania, spotted spurge, sicklepod, and velvetleaf and cotton yields with 0.8 kg ai ha−1 of clomazone plus fluometuron or 0.6 kg ha−1 of clomazone plus pendimethalin plus fluometuron equalled or exceeded that from the standard herbicide programs. POST-directed application of methazole at 0.8 kg ai ha−1 plus MSMA at 2.2 kg ae ha−1 increased sicklepod and morningglory control and cotton yield. Clomazone applied PRE at 0.6 kg ha−1 with fluometuron controlled broadleaf signalgrass, goosegrass, large crabgrass, prickly sida, and smooth pigweed equally with that of standard treatments of trifluralin or trifluralin plus norflurazon PPI and fluometuron PRE, whereas pitted morningglory control and cotton yield with clomazone plus fluometuron exceeded that with the standards.

An experiment was conducted at four locations during 1990 and 1991 to determine the response of cotton to 1.12 kg ai ha-1 of clomazone applied PPI in combination with in-furrow application of 0.50 kg ai ha-1 of aldicarb plus 0.14, 0.28, and 0.56 kg ai ha-1 of either disulfoton or phorate. Early-season cotton injury ranged from 15 to 63% with clomazone plus aldicarb. Stands were reduced 31 and 61% at two locations, and yield was reduced 50% at one location. Applied in combination with aldicarb, all three rates of disulfoton and 0.28 and 0.56 kg ha-1 of phorate protected cotton from clomazone injury. Phorate at 0.14 kg ha-1 partially protected cotton from clomazone injury.

Field and greenhouse experiments evaluated interactions of soil-applied insecticides and imazaquin and imazethapyr on growth and development of cotton. Imazaquin and imazethapyr were applied PPI at 0 to 6 and 0 to 16 g ae ha−1, respectively, in the greenhouse (plus a no-insecticide control), and 0 to 72 g ha−1 in the field in combination with aldicarb, disulfoton, and phorate (without a no-insecticide control) applied in the seed furrow. Cotton shoot fresh weight in the greenhouse experiment decreased linearly as herbicide rates increased. Greater reductions in shoot fresh weight were noted with imazaquin than with imazethapyr. Compared with no insecticide, the methylcarbamate insecticide aldicarb and the organophosphate insecticides disulfoton and phorate did not affect cotton response to either herbicide. In the field, cotton injury increased while stand, yield, and maturity decreased as herbicide rates increased. Delayed maturity was due to a lower percentage of bolls produced on sympodia from main stem nodes four to nine. Imazaquin caused greater injury, greater reductions in stand, greater delays in maturity, and lower yields than did imazethapyr. Earlier maturity was noted with aldicarb-treated cotton. Compared with aldicarb, disulfoton and phorate did not alter cotton response to imazaquin or imazethapyr.

Disulfoton and phorate applied in the seed furrow greatly reduce clomazone phytotoxicity to cotton in the field, whereas aldicarb does not. An experiment was conducted to determine the effect of aldicarb, disulfoton, and phorate on 14C-clomazone absorption, translocation, and metabolism by cotton grown in a sandy loam soil. Clomazone at 0.87 μg g−1 of soil alone or in combination with aldicarb at 0.6 μg g−1 of soil reduced cotton root and shoot growth 26 to 33%. Root and shoot growth were not reduced by clomazone plus disulfoton or phorate at 0.6 μg g−1 of soil. Protection of cotton against injury by clomazone was not explained by reduced absorption or translocation of clomazone or a metabolite to the shoot. Clomazone metabolism was reduced by disulfoton and phorate, thus indicating a clomazone metabolite may be more toxic to cotton.

An integrated approach to the management of the sorghum shootfly (to keep its population below economic injury level) has been a major thrust of sorghum research workers during the last one and a half decades. The necessary foundation has been laid by detailed studies on the biology of the pest and a fairly thorough understanding of the effect of different ecological factors on the shootfly population. Based on seasonal incidence studies, sowing immediately after the onset of monsoon has proved to be effective for avoiding shootfly damage.

A large number of high yielding and shootfly-resistant derivatives have been developed by crossing agronomically desirable exotic lines with identified resistant sources. The new hybrids and varieties released recently possess low to moderate levels of resistance.

Parasites and predators affecting different stages of the shootfly have been identified but utilization of these natural enemies for the biological control of the pest is yet to be undertaken.

Carbofuran seed treatment has proved to be highly effective for protecting sorghum from shootfly damage. Some other insecticides have also been found to be promising.

Based on the control methods available at present, an integrated package has been recommended for implementation in traditional sorghum growing areas of the country.