Water status traits have been proposed as criteria for drought tolerance improvement in several species especially in bread wheat, oat and barley. In order to test this possibility in durum wheat, in 1995 and 1997, a collection of 144 genotypes from different geographical origins was cultivated under rainfed Mediterranean conditions in Montpellier (southern France). Relative water content (RWC), osmotic potential (OP), as well as leaf morphological traits, grain yield (GY), total above-ground biomass and harvest index (HI) were measured. Large genotypic variations were observed for all traits. Differences between the 2 years were also noticed for all the traits which could be attributed to differences in water availability between the 2 growing seasons. The correlation analysis has revealed significant positive associations between water status traits and both GY and HI within and across years. These results suggest that genotypes which can maintain higher water status and then higher transpiration rates during grain filling, are more efficient in dry matter partitioning to grain and hence higher yield. Heritabilities of water status characters were higher than those of productivity traits. Our results emphasized the value of RWC and OP as criteria for durum wheat grain yield improvement under rainfed Mediterranean conditions. Relationships between RWC, OP and flag leaf morphological traits are also discussed.

1. Three experiments are reported in which magnesium metabolism trials were carried out with milking cows that had been changed abruptly from typical winter rations to herbage cut freshly from swards at an early or at a more mature stage of growth and fed in the stall.

2. The intake of herbage magnesium by individual animals varied from 9·5 to 15·2 g./day. Much of this variation was, however, associated more with differences in the palatability and dry-matter content of the herbage offered than with individual differences in appetite.

3. The mean proportion of the ingested herbage magnesium excreted in the faeces was similar in all three experiments, being 82·3, 83·0 and 82·4% respectively. With any given sward there were, however, wide variations between individual animals in their utilization of herbage magnesium.

4. The supply of ‘available’ magnesium to animals fed cut herbage in the stall varied from 0·5 to 4·2 g./day, as compared with values of from 2·6 to 10·5 g./day obtained previously (Rook et al. 1958) with cattle fed typical winter rations. In spite of this lower intake of ‘available’ magnesium, the animals maintained a positive magnesium balance.

For seed crops other than cereals there have been comparatively few comprehensive field studies which have analysed the physiological basis of the effects of genotype and agronomic treatments on growth, development and yield. Milbourn & Hardwick (1968), working on vining peas (Pisum sativum L.), have recently shown that pod growth rate and the yield of seeds are not simple functions of leaf area index and that sourc es of materials other than the leaves are important in affecting the yields of pods and seeds. Furthermore, Flinn & Pato (1970) have shown that the pods themselves can contribute to assimilation in the field pea (Pisum arvense L.). Field experiments carried out at Cambridge in 1968 and 1969 have produced evidence on the relative importance of leaves and other photosynthetic tissues in determining the yields of pods and seeds in another seed crop, namely oilseed rape. The experiment in 1969 is described and discussed below.

The effects of adding a culture extract from Aspergillus oryzae (AO) to the fermentation of a basal ration were investigated using the rumen simulation technique (Rusitec). The ration consisted of hay, barley, molasses, fishmeal and a minerals/vitamins mixture, at 500, 299–5, 100, 91 and 9–5 g/kg dry matter respectively. AO eliminated the transient fall in pH that occurred following the addition of substrate to Rusitec, but had no influence on the buffering capacity of the medium. Increases in the acetate: propionate ratio and in the proportion of butyrate in the fermentation products were observed in vessels receiving AO. Ammonia concentrations increased by over 30%. The number of total viable bacteria almost doubled and the cellulolytic population increased three-fold in vessels receiving AO. Protozoal numbers were reduced by almost 45% by AO. The proportion of methane in the headspace gas collected from the vessels during the day was significantly (P < 0·01) lower in vessels receiving AO. These results demonstrate that AO has a marked effect on the microbial population and the stoichiometry of the rumen fermentation, and that these changes can be seen in an in vitro system.

Two years with contrasting weather, 1976 and 1977, were used to investigate the effects of nitrogen fertilizer on the uptake of nitrogen by crops of winter wheat and on the rate of photosynthesis of flag leaves. The studies involved a number of crops which received various amounts of nitrogen fertilizer at various stages of growth. Weekly measurements were made of the amount of nitrogen in the crops and its distribution within the plant, while a mobile gas exchange system measured the photosynthesis of individual flag leaves.

While the treatments imposed in 1976 resulted in different concentrations of nitrogen in the flag leaves, the maximum rate of photosynthesis and its efficiency in dull light were both unaffected when leaves of the same age were compared. The 2 years gave contrasting results in terms of the amounts of nitrogen taken up during grain growth: in the very dry summer of 1976 it could account for only 25% of the nitrogen in the ear at harvest while in 1977 it could account for almost 50%. These different amounts of translocation to the grain were accompanied by different photosynthetic behaviours of the flag leaves and our analysis shows that, in all treatments, the maximum rate of photosynthesis after anthesis was related to the fractional loss of nitrogen from the leaves.

The implications of these results for farming practice and for techniques used to investigate the effects of nitrogen on photosynthesis are discussed.

The effects of supplementation of a barley-barley straw diet containing 4 mg copper (Cu)/kg dry matter (D.M.) either with 5 mg molybdenum (Mo)/kg D.M. or with 500 mg iron (Fe)/kg D.M. on the Cu status, growth and clinical condition of calves are described. Liver and plasma Cu concentrations declined rapidly within 12–16 weeks to levels indicating severe Cu deficiency in both the Mo- and Fe-supplemented groups. The changes in Cu status were similar for both supplements and did not differ significantly from each other. Clinical signs of Cu deficiency, as indicated by reduced growth rate and changes in hair texture and colour, were evident after 16–20 weeks only in animals on the Mosupplemented diet. The reduced growth rate was accompanied by a decreased food intake and reduced efficiency of food utilization. The Mo-supplemented animals also exhibited an altered Fe metabolism, particularly when the growth rate was affected. The results confirm earlier reports that the effects of Mo on growth and clinical condition are independent of its effect on Cu status and suggest that the altered production might be due to effects on the control of food intake, food utilization, or Fe metabolism.

An experiment at Sutton Bonington in 1971 tested the effects of sowing and harvesting dates on yield of sugar beet. Seed sown on 28 March germinated slowly in the cold soil and crop establishment was poor. From early April until the beginning of May the loss in yield of sugar consequent upon delaying sowing by 1 week was 0·4 t/ha. For later sowings the yield loss was greater. During the harvest period the rate of increase in sugar yield was the same for all sowings. Yields of crops sown and harvested on different dates were closely correlated withthe amount of solar radiation intercepted by the leaf canopy.

1. The energy metabolism and heat emission of two steers was determined at the maintenance level of nutrition in an environment of – 5, 5, 15, 25 and 35° C. and at a submaintenance level of nutrition in environments of – 5 and 19° C.

2. Shivering was observed at temperatures of – 5 and + 5° C. in both animals. Respiratory distress occurred at 35° C. being more severe in one animal than in the other. The animals stood for longer periods at the extreme temperatures.

3. The digestibility of food increased slightly as environmental temperature increased. Losses of energy as methane and in urine did not change in response to environmental temperature.

The current study was designed to determine the effect of roughage to concentrate ratio (R : C) on rumen pH, fermentation and bacterial population in dairy steers. Four rumen fistulated dairy steers (170±20 kg) were randomly assigned according to a 4×4 Latin square design, in which the steers were fed with four dietary treatments with different R : C ratios of 0·8 : 0·2, 0·6 : 0·4, 0·4 : 0·6 and 0·2 : 0·8, respectively. All animals were kept in individual pens and received feed according to the respective R : C ratios at 0·025 body weight (BW)/d; urea-treated rice straw (prepared using 3·5 kg urea+100 kg water sprayed onto 100 kg of rice straw) was used as a roughage source. The experiment was conducted for four periods of 21 days each. During the first 14 days, feed intake was measured and the animals were then moved to metabolism crates for total urine and faecal collection for 7 days. Total dry matter intake (DMI) was similar among treatments. Energy intake increased as the proportion of concentrate in the diet increased. Apparent digestibilities of dry matter (DM), organic matter (OM) and crude protein (CP) were improved, while neutral detergent fibre (NDF) and acid detergent fibre (ADF) were reduced when the levels of concentrate increased. A decreasing ratio of R : C reduced rumen pH linearly, from 6·4 to 5·9 at 0·2 : 0·8. High levels of concentrate impacted on volatile fatty acids (VFA) molar proportions and decreased acetate (C2) linearly, while propionate (C3) was increased, leading to decreased C2 : C3 ratio. Numbers of protozoa, fungi and proteolytic bacteria were not affected by R : C ratio. Cellulolytic bacteria decreased linearly while amylolytic bacteria increased linearly with 0·60 and 0·80 concentrates. Quantitative polymerase chain reaction (qPCR) based on 16S RNA revealed that Fibrobacter succinogenes numbers were increased when steers were fed with R : C ratio of 0·8 : 0·2. Conjugated linoleic acid (CLA)-producing bacteria, especially those of Butyrivibrio fibrisolvens, increased linearly with R : C ratios of 0·8 : 0·2 and 0·6 : 0·4, while Megasphaera elsdenii, a lactate-utilizing bacterium and reported producer of trans-10, cis-12 CLA increased linearly with R : C ratio of 0·8 : 0·2. In addition, microbial CP synthesis increased quadratically when steers were fed high levels of concentrate. However, the efficiency microbial N synthesis (EMNS) based on OM, truly digested in the rumen, was not affected by different R : C ratios. From the current study, it can be concluded that roughage to concentrate ratio of 0·4 : 0·6 had positive effects for the creation of healthy rumen (rumen pH and ecology), and improved energy intake and rumen fermentation, particularly propionic acid and microbial protein synthesis, in dairy steers fed urea-treated rice straw as a roughage source.

Field experiments were carried out in Brittany (Western France) in 1993 to measure ammonia losses from surface-applied pig and cattle slurry. Experiments were conducted on grass, stubble (wheat and maize) and arable land using a wind tunnel system. Ammonia losses were followed during periods ranging from 20 to 96 h after slurry application. Rates of slurry applied varied from 40 to 200 m3/ha. In two experiments, losses from cattle slurry were respectively 75 and 54% of the ammoniacal nitrogen applied in the slurry. Ammonia emissions from pig slurry applied at a rate of 40 m3/ha, during spring and summer experiments, were higher on grass (45–63% of the total ammoniacal nitrogen applied) than on wheat stubble (37–45%). On average, 75% of the total loss in all experiments occurred within the first 15 h after spreading. Significant correlations were found between ammonia losses (kg N/ha) and mean soil temperature and slurry dry matter content (%) using simple linear regressions and stepwise procedures. The time of application was also found to influence the magnitude of ammonia loss: 83% of the total loss occurred within 6 h when the slurry was applied at midday compared with 42% when it was applied in the evening.

Thirty-six mature Finnish Landrace × Dorset Horn ewes, each suckling two lambs, were used in a comparative slaughter experiment to measure changes in body tissues during early lactation. Two levels of body fatness at lambing were established by giving ewes a complete diet containing 10 MJ metabolizable energy (ME) and 139 g crude protein (CP)/kg d.m. either close to requirements or ad libitum during the second half of pregnancy. In lactation half the ewes in each group were given a complete diet containing either 90 (diet A) or 60 (diet B) % milled hay ad libitum. These diets contained 7·9 and 9·2 MJ ME and 121 and 132 g CP/kg d.m. respectively.

Ewes fed at the two levels in pregnancy contained 8·4 and 19·6 kg chemically determined fat 5 days after lambing but had similar amounts of body protein, ash and water. Over 6 weeks of lactation ewes given diet A lost 60 and 69% of these weights of fat respectively, while ewes given diet B gained 5% and lost 30% respectively. Up to 26 g of body protein was lost daily from ewes given diet A but none from ewes on diet B. During early lactation the weight of the empty digestive tract increased while the weights of most other body components, particularly the carcass, decreased. The ratio of body energy change to live-weight change varied from 24 to 90 MJ/kg. Thus live-weight change did not accurately reflect relative or absolute changes in body energy.

Voluntary food intake was greater for ewes given the high-energy diet (B) than for those given diet A and was depressed in the fatter ewes. Differences in intake could be explained by the effects of body fatness and diet on the weight of gut contents. Milk yield was not significantly affected by body fat reserves but was higher on diet B than A. Fat content of milk was higher and protein content lower for ewes with the higher fat reserves at lambing.

As the contribution of fat loss to energy available for milk synthesis increased there appeared to be a reduction in the energetic efficiency of milk synthesis. A number of possible reasons for this are discussed.

Near infrared spectroscopy (NIRS) is widely used in the flour milling industry for rapid determination of moisture and protein in wheat. However, these measurements give little indication of the nutritive value of wheat when fed to poultry or pigs. Accurate estimates of nutritive value require specialist facilities and are time-consuming and costly. Accordingly, prediction from chemical or NIRS measurements would be of some considerable benefit. In the current study 160 samples of wheat, representing 24 different varieties, were used to generate NIRS calibration equations for chemical, nutritive and agronomic characteristics. Predictions of chemical constituents in wheat were very accurate. Coefficients of determination (r2) were 0·94 for dry matter, 0·90 for crude protein, 0·97 for ash, 0·78 for starch and 0·98 for oil. True metabolizable energy in broiler chickens was predicted more accurately (r2 = 0·52 for adult birds, 0·74 for young birds) than apparent metabolizable energy (r2 = 0·45). Digestible energy (r2 = 0·17) and nitrogen digestibility (r = 0·22) in pigs were not predicted very accurately on a smaller subset (n = 33). Agronomic characteristics were predicted very accurately (r2 = 0·98 hardness, 0·80 bushel weight, 0·99 thousand-grain weight). Predictions of nutritive value of wheat from chemical or agronomic characteristics are very inaccurate, since coefficients of determination vary from zero to 0·25. It is concluded that NIRS can accurately estimate the chemical composition of wheat, but accurate prediction of nutritive value is reduced by animal variation. Nevertheless, NIRS is potentially more reliable for assessing nutritive value than chemical composition or agronomic characteristics.

No attempt will be made to give a detailed summary of all the findings of the present experiment. It is rather our purpose to draw attention to the main principles emerging.

1. The influence of extremes of high and low planes of nutrition during the first 16 weeks of post-natal life upon the growth in body proportions and in anatomical composition has been studied experimentally in six pairs of closely inbred pigs. Quantitative differences in nutrition operative from birth have resulted in an average live weight at 16 weeks of 113 lb. in the High-Plane and 37 lb. in the Low-Plane animals.

2. In body proportions, the head, ears, neck, legs, and body length are penalized relatively less by inadequate nutrition than are body depth, loin, and hindquarters. Conversely, good nutrition favours most the latter characters. These effects upon body form are similarly evident i n the gross weight of the different anatomical regions involved. Low-Plane animals to a large extent retain the proportions of the juvenile and High-Plane approach the conformation of the adult

The goal of the present study was to assess the impact of selected soil protection measures on soil erosion and retention of rainwater in a 1·14 km2 watershed used for agriculture in the north-east of Austria. Watershed conditions under conventional tillage (CT), no-till (NT) and under grassland use were simulated using the Water Erosion Prediction Project (WEPP) soil erosion model. The period 1961–90 was used as a reference and results were compared to future Intergovernmental Panel on Climate Change (IPCC) scenarios A1B and A2 (2040–60).

The simulations for the NT and grassland options suggested runoff would decrease by 38 and 75%, respectively, under the current climatic conditions. The simulation results suggest that, under future climate scenarios, the effectiveness of the selected soil conservation measures with respect to runoff will be similar, or decreased by 16–53%.

The actual average net soil losses in the watershed varied from 2·57 t/ha/yr for conventional soil management systems to 0.01 t/ha/yr for grassland. This corresponds to a maximum average annual loss of about 0·2 mm, which is considered to be the average annual soil formation rate and therefore an acceptable soil loss. The current soil/land use does not exceed this limit, with most of the erosion occurring during spring time. Under future climate scenarios, the simulations suggested that CT would either decrease soil erosion by up to 55% or increase it by up to 56%. Under these conditions, the acceptable limits will partly be exceeded. The simulations of NT suggested this would reduce annual soil loss rates (compared to CT) to 0·2 and 1·4 t/ha, i.e. about the same or slightly higher than for NT under actual conditions. The simulation of conversion to grassland suggested soil erosion was almost completely prevented.

The selected soil conservation methods maintain their protective effect on soil resources, independent of the climate scenario. Therefore, with small adaptations, they can also be recommended as sustainable soil/land management systems under future climatic conditions.

However, based on the available climate scenarios, climate-induced changes in the frequency and intensity of heavy rainstorms were only considered in a limited way in the present work. As the general future trend indicates a strong increase of rainstorms with high intensity during summer months, the results of the present study may be too optimistic.

Field experiments with the South American grain crop, quinoa, were carried out at two sites in Denmark in 1988–90 in order to study the effects of varying the nitrogen fertilization rate, seed rate, row spacing, harvesting method and harvest date. Although there was a significant yield increase when the amount of nitrogen fertilizer was increased from 40 to 160 kg N/ha, quinoa seems to be well adapted to poor soils. Yield decreased by 24–1% when the nitrogen supply was reduced from 160 to 40 kg N/ha, while the yield decrease was 120 and 2–7% when the nitrogen supply was reduced to 80 and 120 kg/ha, respectively.

A model expressing yield as a function of plant density, for three experiments analysed as one, showed an optimal plant density with respect to yield of 327 ± 220 plants/m2. This plant density was the top point of the curve relating yield to plant density. However, the large standard deviation indicates that apparently a rather wide range of plant densities would provide similar yields. When the row spacing was varied, it was shown that plots with a row spacing of 50 cm, which were hoed, gave a higher yield than plots with 25 or 12·5 cm row spacing, which were unhoed. There was an overall increase in yield when changing from combined harvesting to swathing; however, when comparing the yields from the optimal harvest dates for the two methods, no yield differences could be detected. The optimal harvest date for swathing was found to be the stage when the inflorescences start to turn brown, whereas for combining, the optimal harvest date was the stage when most of the inflorescences are already brown.

Ewes. There was some variation from year to year in the times of onset and end of the breeding season; on the average it was evenly spaced on either side of the shortest day. Occasionally ewes were served and became pregnant at times well outside the normal limits of the breeding season. Lambs were allowed to suckle the ewes for as long as they would; when lambing occurred more than about 100 days from the start of the season there was no delay in onset of heat in the ewe. When lambing was later there was some delay, but the duration of the lactation anoestrum shortened to a minimum near the middle of the season and then lengthened again.

In the first half of the season the period of the oestrous cycle lengthened slowly and steadily; in the second part it became more variable, there was first a slight shortening, but upon the whole it continued to increase in length. From the start of the season the frequency of twinning increased quickly to a peak in about November and then declined for the rest of the season. At the end of the season there was a high proportion of services not fertile.

Lambs. Growth in the first 2 months was greatest in those born in May; in the fourth to sixth months it was greatest in the earliest born and least in the latest, the highest weight at 6 months old being reached by those earliest born.

The response of winter wheat cv. Maris Huntsman to waterlogging was studied in two experiments in soil columns outdoors. Winter waterlogging treatments increased nodal root production and the proportion of aerenchyma within roots, but caused chlorosis and premature senescence of leaves, and decreased tillering. For all treatments, grain losses were much less than expected from the extent of tiller loss in winter; losses after single waterlogging events ranged from 2% (after 47 days with the water-table at 5 cm) to 16% (after 80 days with the water-table at the soil surface). Yield losses after three waterloggings at the seedling, tillering and stem elongation stages of growth were additive, and totalled 19%. In many treatments, grain loss was associated with lighter individual grain weights, suggesting that the size of the root system or efficiency of water and nutrient uptake by roots at the later stages of growth may have been less after earlier waterlogging. The importance of nitrogen fertilizer in maintaining a satisfactory plant nitrogen status was shown when nitrogen was with held before a 3-week waterlogging treatment during stem elongation; tiller and floret survival was subsequently greatly restricted and grain yields decreased 22% below those of plants waterlogged at the same stage of growth but supplied with nitrogen.

The soil nitrate content has been determined at fortnightly intervals on bare fallow plots and on plots under crops and natural vegetation in the forest and savannah regions of Ghana. Nitrifiable nitrogen, ammonium and soil moisture contents have also been determined. It has been found that the changes in nitrate content are related to changes in the content of nitrifiable nitrogen. During the dry season, a partial sterilization of the soil occurs and consequently the content of nitrifiable nitrogen rises. This nitrifiable nitrogen is slowly converted to nitrate in the dry season and rapidly converted once the rains commence. Nitrate is then lost from the soil by leaching and denitrification. Under bare fallow conditions leaching is the most important cause of nitrate loss, but it is probable that denitrification makes some contribution on cropped soils and those under permanent vegetation. There is no indication of microbial nitrate absorption under a crop. This is presumably due to the rapidity with which nitrate is lost by leaching and denitrification, and to the greater quantity of organic matter required in these free-draining tropical soils than in most temperate ones to produce an equivalent absorption of nitrate. The high levels of nitrate and nitrifiable nitrogen which are found under natural forest probably represent an equilibrium level typical of a cycle in which nitrogen fixation and nitrification as well as nitrate losses proceed very rapidly. Natural savannah grassland suppresses nitrogen mineralization in the field almost entirely, but the nitrifiable nitrogen content is at times high, in spite of the very high carbon/nitrogen ratio of the soil of over 20. This confirms the view that little nitrate absorption takes place in spite of the presence of what might be considered excess carbonaceous material. The suppression of mineralization under grass is considered due to an excretion of the plant roots which is toxic to the nitrification process. The failure of organic matter to build up in these savannah soils, although mineralization is suppressed, is most probably associated with their low general fertility.

Sixty Holstein/Friesian dairy cows, 28 of high genetic merit and 32 of medium genetic merit, were used in a continuous design, 2 (cow genotypes)×4 (concentrate proportion in diet) factorial experiment. High and medium merit animals had Predicted Transmitting Abilities for milk fat plus protein yield, calculated using 1995 as the base year (PTA95 fat plus protein), of 43·3 kg and 1·0 kg respectively. Concentrate proportions in the diet were 0·37, 0·48, 0·59 and 0·70 of total dry matter (DM), with the remainder of the diet being grass silage. During this milk production trial, 24 of these animals, 12 from each genetic merit, representing three animals from each concentrate treatment, were subject to ration digestibility, and nitrogen and energy utilization studies. In addition, the efficiency of energy utilization during the milk production trial was calculated.

There were no genotype×concentrate level interactions for any of the variables measured (P>0·05). Neither genetic merit nor concentrate proportion in the diet influenced the digestibility of either the DM or energy components of the ration (P>0·05). When expressed as a proportion of nitrogen intake, medium merit cows exhibited a higher urinary nitrogen output and a lower milk nitrogen output than the high merit cows. Methane energy output, when expressed as a proportion of gross energy intake, was higher for the medium than high merit cows (P<0·05), while urinary energy output tended to decrease with increasing proportion of concentrate in the diet (P<0·05). In the calorimetric studies, neither heat energy production, milk energy output and energy retained, when expressed as a proportion of metabolizable energy intake, nor the efficiency of lactation (kl), were affected by either cow genotype or concentrate proportion in the diet (P>0·05). However when kl was calculated using the production data from the milk production trial the high merit cows were found to have significantly higher kl values than the medium merit cows (0·64 v. 0·59, P<0·05) while k l tended to fall with increasing proportion of concentrate in the ration (P<0·05). However in view of the many assumptions which were used in these latter calculations, a cautious interpretation is required.

1. The heat emission of two closely clipped sheep was studied in forty experiments in which three feeding levels were employed and in which environmental temperatures ranged from 8 to 38° C.

2. The sheep shivered at temperatures below 23° C., but the first reaction to cold was a corrugation of the skin referable to contraction of the fascial muscles.

3. As environmental temperature increased voluntary water consumption and urinary volume increased markedly. Only a small degree of cooling resulted from this warming of large quantities of water through a relatively small temperature gradient.

4. The basal loss of heat by vaporization of water was 316 Cal./m.2/24 hr. in one sheep (3) and 307 Cal./ m.2/24 hr. in the other (23). Increased vaporization occurred at environmental temperatures of 31° C. for the experiments at the low-feeding level, 27° C. at the medium level and 26° C. at the high level. The water-vapour loss increased subsequently by 87 Cal./m.2/24 hr./° C. change in environmental temperature in sheep 3 and by 88 Cal./m.2/24 hr./° C. in sheep 23. Respiratory frequencies increased with increasing heat loss by vaporization. Some evidence suggests that when the water-vapour loss was high the excretion of potassium through the skin was increased.