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Transgenic expression of sorghum DREB2 in rice improves tolerance and yield under water limitation

Published online by Cambridge University Press:  08 November 2010

P. BIHANI ,
B. CHAR  and
S. BHARGAVA
P. BIHANI
Affiliation:
Mahyco Research Centre, Jalna, India
B. CHAR
Affiliation:
Mahyco Research Centre, Jalna, India
S. BHARGAVA*
Affiliation:
Botany Department, University of Pune, Pune, India
*
*To whom all correspondence should be addressed. Email: [email protected]
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Summary

Plant-specific transcription factors belonging to the dehydration response element binding (DREB)/C-repeat binding factor (CBF) subfamily of the AP2/EREBP family specifically interact with dehydration-responsive elements (DRE)/C-repeat (CRT) and control the expression of many stress-inducible genes in plants. Two major subgroups of DREB proteins are represented by DREB1 and DREB2, which are induced specifically under cold and drought/salt stress, respectively. A DREB2 transcription factor gene from sorghum, SbDREB2 was identified and cloned in binary vectors, such that it was driven either by a constitutive CaMV35S promoter or a stress-inducible rd29A promoter. These gene constructs were transferred into rice through Agrobacterium tumefaciens-mediated transformation. Expression patterns of the native DREB gene (OsDREB2) and the transgene (SbDREB2) were similar. Both genes showed induction at 1 h exposure to drought, after which expression gradually dropped to basal levels by 24 h. Constitutive expression of SbDREB2 led to pleiotropic effects in rice and these transgenics did not set seed. The rd29A: SbDREB2 rice plants set seed and the grains collected from primary transformants were sown to raise T1 plants. The drought-stressed rd29A: SbDREB2 transgenics showed a significantly higher number of panicles as compared to the wild-type rice plants. Other phenological and agronomic traits were not affected in wild-type and rd29A: SbDREB2 transgenic rice.

Type
Crops and Soils
Information
The Journal of Agricultural Science , Volume 149 , Issue 1 , February 2011 , pp. 95 - 101
Copyright
Copyright © Cambridge University Press 2010

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References

REFERENCES

Agarwal, P., Agarwal, P. K., Nair, S., Sopory, S. K. & Reddy, M. K. (2007). Stress inducible DREB2A transcription factor from Pennisetum glaucum is a phosphoprotein and its phosphorylation negatively regulates its DNA-binding activity. Molecular Genetics and Genomics 277, 189–198.CrossRefGoogle ScholarPubMed
Azhakanandam, K., McCabe, M. S., Power, J. B., Lowe, K. C., Cocking, E. C. & Davey, M. R. (2000). T-DNA Transfer, integration, expression and inheritance in rice: effects of plant genotype and Agrobacterium super-virulence. Journal of Plant Physiology 157, 429–439.CrossRefGoogle Scholar
Babu, R. C., Nguyen, B. D., Chamarerk, V., Shanmugasundaram, P., Chezhian, P., Jeyaprakash, P., Ganesh, S. K., Palachamy, A., Sadasivam, S., Sarkarung, S., Wade, L. J. & Nguyen, H. T. (2003). Genetic analysis of drought resistance in rice by molecular markers: association between secondary traits and field performance. Crop Science 43, 1457–1469.CrossRefGoogle Scholar
Bartels, D. & Souer, E. (2004). Molecular responses of higher plants to dehydration. In Plant Responses to Abiotic Stress (Eds Hirt, H. & Shinozaki, K.), pp. 9–38. Berlin: Springer-Verlag.Google Scholar
Chen, J.-Q., Meng, X.-P., Zhang, Y., Xia, M. & Wang, X.-P. (2008). Over-expression of OsDREB genes lead to enhanced drought tolerance in rice. Biotechnology Letters 30, 2191–2198.CrossRefGoogle ScholarPubMed
Chinnusamy, V., Schumaker, K. & Zhu, J. K. (2004). Molecular genetic perspectives on cross talk and specificity in abiotic stress signalling in plants. Journal of Experimental Botany 55, 225–236.CrossRefGoogle ScholarPubMed
Dellaporta, S. L., Wood, J. & Hicks, J. B. (1983). A plant DNA minipreparation: version II. Plant Molecular Biology Reporter 1, 19–21.CrossRefGoogle Scholar
Egawa, C., Kobayashi, F., Ishibashi, M., Nakamura, T., Nakamura, C. & Takumi, S. (2006). Differential regulation of transcript accumulation and alternative splicing of DREB2 homolog under abiotic stress conditions in common wheat. Genes and Genetic System 81, 77–91.CrossRefGoogle ScholarPubMed
Fowler, S. & Thomashow, M. F. (2002). Arabidopsis transcriptome profiling indicates the multiple regulatory pathways are activated during cold acclimation in addition to CBF cold response pathway. Plant Cell 14, 1675–1690.CrossRefGoogle ScholarPubMed
Huang, D., Wu, W., Abrams, S. R. & Cutler, A. J. (2008). The relationship of drought-related gene expression in Arabidopsis thaliana to hormonal and environmental factors. Journal of Experimental Botany 59, 2991–3007.CrossRefGoogle ScholarPubMed
Kasuga, M., Liu, Q., Miura, S., Yamaguchi-Shinozaki, K. & Shinozaki, K. (1999). Improving plant drought, salt, and freezing tolerance by gene transfer of a single stress-inducible transcription factor. Nature Biotechnology 17, 287–291.CrossRefGoogle ScholarPubMed
Kushalappa, K. M., Mattoo, A. K. & Vijayraghavan, U. (2000). A spectrum of genes expressed during early stages of rice panicle and flower development. Journal of Genetics 79, 25–32.CrossRefGoogle Scholar
Maruyama, K., Sakuma, Y., Kasuga, M., Ito, Y., Seki, M., Goda, H., Shimada, Y., Yoshida, S., Shinozaki, K. & Yamaguchi-Shinozaki, K. (2004). Identification of cold-inducible downstream genes of the Arabidopsis DREB1A/CBF3 transcriptional factor using two microarray systems. Plant Journal 38, 982–993.CrossRefGoogle ScholarPubMed
Nakano, T., Suzuki, K., Fujimura, T. & Shinshi, H. (2006). Genome-wide analysis of the ERF gene family in Arabidopsis and rice. Plant Physiology 140, 411–432.CrossRefGoogle ScholarPubMed
Oh, S.-J., Song, S. I., Kim, Y. S., Jang, H.-J., Kim, S. Y., Kim, M., Kim, Y.-K., Nahm, B. H. & Kim, J.-K. (2005). Arabidopsis CBF3/DREB1A and ABF3 in transgenic rice increased tolerance to abiotic stress without stunting growth. Plant Physiology 138, 341–351.CrossRefGoogle ScholarPubMed
Pastori, G. M. & Foyer, C. H. (2002). Common components, networks and pathways of cross-tolerance to stress. The central role of ‘Redox’ and abscisic acid-mediated controls. Plant Physiology 129, 460–468.CrossRefGoogle ScholarPubMed
Pellegrineschi, A., Reynolds, M., Pacheco, M., Brito, R. M., Almeraya, R., Yamaguchi-Shinozaki, K. & Hoisington, D. (2004). Stress-induced expression in wheat of the Arabidopsis thaliana DREB1A gene delays water stress symptoms under greenhouse conditions. Genome 47, 493–500.CrossRefGoogle ScholarPubMed
Qin, F., Kakimoto, M., Sakuma, Y., Maruyama, K., Osakabe, Y., Tran, L.-S. P., Shinozaki, K. & Yamaguchi Shinozaki, K. (2007). Regulation and functional analysis of ZmDREB2A in response to drought and heat stress in Zea mays L. Plant Journal 50, 54–69.CrossRefGoogle ScholarPubMed
Sakuma, Y., Maruyama, K., Qin, F., Osakabe, Y., Shinozaki, K. & Yamaguchi-Shinozaki, K. (2006). Dual function of an Arabidopsis transcription factor DREB2A in water-stress-responsive and heat-stress-responsive gene expression. Proceedings of the National Academy of Sciences USA 103, 18822–18827.CrossRefGoogle ScholarPubMed
Thomashow, M. F. (2001). So what's new in the field of plant cold acclimation? Lots! Plant Physiology 125, 89–93.CrossRefGoogle ScholarPubMed
Yamaguchi-Shinozaki, K. & Shinozaki, K. (1994). A novel cis-acting element in an Arabidopsis gene is involved in responsiveness to drought, low-temperature, or high-salt stress. Plant Cell 6, 251–264.Google ScholarPubMed
Yamaguchi-Shinozaki, K. & Shinozaki, K. (2005). Organization of cis-acting regulatory elements in osmotic- and cold-stress-responsive promoters. Trends in Plant Science 10, 88–94.CrossRefGoogle ScholarPubMed
Yamaguchi-Shinozaki, K. & Shinozaki, K. (2006). Transcriptional regulatory networks in cellular responses and tolerance to dehydration and cold stresses. Annual Review of Plant Biology 57, 781–803.CrossRefGoogle ScholarPubMed