Introduction. The grapefruit tree has a long production period. In the Jordan Valley, harvest lasts from November to March. Many Jordanian farmers tend to harvest their fruit early to get high prices, regardless of its quality. Thus, the effect of the harvesting date on fruit quality of grapefruit cv. ‘Red Blush’ was evaluated under Jordan Valley conditions for two seasons, 2002 and 2003. Materials and methods. Fruit quality of uniform 25-year-old grapefruit cv. ‘Red Blush’ trees budded on sour orange rootstock was studied during the 2002 and 2003 seasons. Fruits were harvested from October 15 in both seasons with 15-day intervals with a total of eight harvests. The studied data were total soluble solids (TSS), titratable acidity (TA), the [TSS/TA] ratio and juice percentage. Results and discussion. Fruit total soluble solids and fruit juice percentages were at a minimal level during early harvesting dates and increased with time, reaching the maximum level on January 1 for TSS and January 15 for fruit juice percentage in the first season and on December 15 and February 1, respectively, in the second season, after that TSS decreased. Fruit titratable acidity values were at maximal levels during early harvesting dates (October 15) and decreased with time, reaching the minimum level on February 1 in the first season and on January 15 in the second season. The [TSS/TA] ratio ranged from 5.5 to 6.0 between January 1 and February 1 in the first season, and between December 1 and January 15 in the second season. Conclusion. Our study displayed that Jordan Valley growers are harvesting their grapefruits much too early to provide acceptable eating quality fruits to local markets.

Introduction. The date palm tree is one of the fruit trees most cultivated in the Arabian Gulf States. Bioregulators were used for the quality and productivity improvement of date palm tree fruits. A study was conducted to assess the relative effectiveness of GA3, NAA, ethephon and a mixture of growth regulators on some fruit characteristics and productivity of the Barhee date palm tree cultivar. Materials and methods. Five selected female uniform date palm trees of the cultivar Barhee were pollinated on March 5-15, 1994, 1995 and 1996, by placing eight fresh male strands on the female spadix center (flower cluster).Ten flower clusters were used on each tree and divided into five groups of two flower clusters. Each of these groups was subjected to one of the following treatments: control (water), 150 mg GA3 × L-1, 100 mg NAA × L-1, 1 000 mg ethephon × L-1 and a mixture of these three growth regulators. Then the fruit setting, fruit flesh and dry matter percentages, the total soluble solids, the fruit ripening, and the fruit weight per bunch and per tree were measured. Results and discussion. The data showed that the application of GA3 or ethephon on flower clusters of Barhee date palm trees had no constant effect on fruit characteristics and productivity of trees. NAA or growth regulator mixture applications on Barhee flower clusters reduced the fruit dry matter and fruit ripening percentages and increased the fruit weight per bunch and per tree. Conclusion. Treatments of Barhee date palm flower clusters, 20 d after pollination, with NAA or the specific growth regulator mixture decreased the dry matter percentage and the fruit ripening and increased the fruit flesh percentage and the date palm tree yield, therefore, these treatments could be recommended to improve the productivity of date palm trees in the region.

Introduction. The chataigne or breadnut, Artocarpus camansi Blanco, has high nutritive value but it is an under-utilised food source. Yield losses due to tree height and limited information on productivity are constraints to commercial production. Our study was undertaken to determine fruit yield and seed yield, the relationship between fruit size and seed yield, and the response of yield to pruning. Materials and methods. Fruit and seed yield data were collected from 1996 to 2002 from three seedling trees established at the University of the West Indies in Trinidad and Tobago in 1993. The trees were pruned in December 1998. Results. The highest fruit mass per tree (139.7 kg), fruit number per tree (126) and seed mass per tree (59 kg) were obtained in 5-year-old trees. Fruit and seed yields were significantly (P < 0.001) lower in the years after pruning. Fruit mass per tree was positively correlated with fruit number (r = 0.99). Seed mass per fruit was positively correlated with seed number per fruit (r = 0.87) and both variables had strong, positive correlations with mean fruit mass (r = 0.83 and r = 0.77, respectively) and fruit volume (r = 0.63 and r = 0.67, respectively). Discussion and conclusions. Chataigne fruit and seed yield potential is greater than originally estimated. Both are strongly related and selection for high seed number per fruit with more effective pollination, disease control and proper tree height management may further increase productivity. In the field, estimated fruit volume is the most practical indicator of seed yield.

Introduction. The objectiveof this study was to examine the relationships among the ripeningattributes of ‘Honeycrisp’ apples at harvest and after storage,and the direct and indirect contributions of these attributes topeel greasiness and the incidence of soft scald and soggy breakdownduring storage using correlation and path-coefficient analyses. Materialsand methods. Fruit were harvested from a commercial orchardat least five times throughout the commercial harvest period during foursubsequent years (2008 to 2011). In two of the years, fruit werestored in air for 3 months at 3 °C and/or in a controlled atmosphere(1–2 kPa O2 + 1–2 kPa CO2) for 6 months at 3 °C. Fruit were analyzedat harvest and after storage. Results and discussion.Negative correlations were detected between internal ethylene concentration(IEC) and soluble solids concentration (SSC) or titratable acidity(TA) (the higher the IEC, the lower the SSC and TA) and a positivecorrelation between firmness and TA (the higher the firmness, thehigher the TA). More peel greasiness and higher incidence of soggybreakdown during storage were associated with lower firmness, SSCand TA. Negative correlations were also detected between the incidence ofsoft scald and IEC or peel greasiness. The results of the path-coefficientanalyses suggest that, in ‘Honeycrisp’, interrelationships amongpostharvest ripening indices and each individual disorder differ. Threepossible path models for the interrelationships among ripening attributes (independent variables)and the incidence of peel greasiness, soft scald and soggy breakdown(dependent variables) are presented.

Introduction. The present study had as objectives to measure the palm leaf divergence angle in the field, to determine the frequency of trees and offshoots showing clockwise and counterclockwise phyllotaxis, to establish the relationship between phyllotaxis of the mother tree and that of its offshoots, and, finally, to determine the effect of the divergence angle value on production. Materials and methods. The vegetal material was composed of four Moroccan cultivars of Phœnix dactylifera L. Observations were conducted in the south of Morocco on 20 trees per cultivar and four offshoots per tree. The divergence angle was measured on adult trees whereas phyllotaxic direction (clockwise or counterclockwise) was noted for both offshoots and adult trees. Results and discussion. This study showed that the phyllotaxic direction of the offshoots presents a clockwise or counterclockwise phyllotaxis independently of the mother plant. The divergence angle varied depending on the cultivar. This character is an interesting criterion, both for the selection of the best adapted cultivars for the marginal date palm growing regions, and for an optimal production.

Introduction. La culture des agrumes est très répandue en Guinée sous forme de vergers decase. La moyenne Guinée (Fouta Djalon), principale région productrice d'oranges, assure environ les deuxtiers de la production nationale et la presque totalité des exportations régionales. Cependant, à la finde 1993, la cercosporiose due à Phaeoramularia angolensis a envahi les zones d'altitude du pays àpartir de foyers primaires situés en moyenne Guinée, entraînant une nette diminution de la production.Matériel et méthodes. Trois enquêtes par région naturelle et par an ont été effectuées dans plusieurs localités du pays pour établir les limites d'extension de la maladie. Des échantillons defeuilles et de fruits suspects, prélevés sur 25 arbres par localité, ont été identifiés en laboratoire. Lavitesse moyenne de progression de la maladie a été estimée. Résultats et discussion. Les observationsfaites en champ et complétées par les travaux de laboratoire entre 1995 et 1998 ont confirmé la présencede la maladie. Les zones agrumicoles situées à plus de 400 m d'altitude ont été particulièrement touchéespar le fléau. Les zones basses et chaudes de la Guinée maritime et de la haute Guinée sont épargnées. Lamaladie progresserait à une vitesse de 23 km $\cdot$ an-1. Conclusion. La cercosporiose desagrumes continue sa progression en Guinée. La presque totalité des vergers du Fouta Djalon est atteinte.La reconversion des zones agrumicoles touchées par la maladie devrait être envisagée par diversificationfruitière et délocalisation des nouvelles plantations vers des zones basses et écologiquement protégées.

Introduction. Litter on the ground is a primary source of Botrytis cinerea inoculum in boysenberry (Rubus hybrid) gardens. The effect of litter management on primary inoculum production, and flower and berry infections was determined. Materials and methods. A series of experiments ranging from laboratory to large-scale field evaluations were conducted in New Zealand during 1997-2002 to evaluate litter management options for control of B. cinerea. The laboratory trial investigated the effect of litter size (shredded vs. unshredded litter) and debris amendments on tissue degradation and B. cinerea colonization. The field trial (four sites) investigated the effect of litter amendments (compost, urea and fungicide) and piling litter on B. cinerea sporulation. In the 4-year commercial-scale study (three properties), the effect of litter treatment (piling, compost and microbial extracts/suspensions) on primary inoculum, flower and berry infections was assessed. Results and discussion. The laboratory trial showed that bark + sewage sludge compost amendment enhanced litter decomposition and reduced B. cinerea sporulation on infected tissue after 8 weeks. The field trial indicated that piling of shredded boysenberry debris was more important than litter amendments in reducing the amount of B. cinerea harbored within the litter. Commercial field-scale evaluation of litter management options verified that piling of shredded litter is the most important step in B. cinerea inoculum control from debris. It also showed that microbial litter amendments (compost, solutions or extracts) can be beneficial. While B. cinerea inoculum control also reduced the amount of flower infections, berry infections at harvest were not affected, indicating that other sources of B. cinerea inoculum contribute to berry infection post-flowering.

Introduction. This protocol can be used as a guide for people who wish to construct their own nursery. It describes the methods for receiving, transplanting, managing and hardening young banana tissue culture plants after the weaning stage. The principle, key advantages, starting plant material and time required are presented. Materials and methods. This part describes the nursery construction, the nursery media, fertiliser and bags, the handling and transplanting of weaned plants into bags (3 steps), and the management schedule for hardening nursery plants (8 steps). Possible troubleshooting is listed. Results. All the characteristics of nursery-hardened plants in bags, ready for transplanting to the field, are formulated.

Introduction. Root-knot nematodes (Meloidogyne sp.) cause significant economic damage to Prunus species in China. One of the most economical and environmentally sustainable methods to reduce the impact of root-knot nematodes is the use of resistant rootstock cultivars. Our aim was to examine resistance to M. incognita and its mechanisms. Materials and methods. Four rootstocks were assessed: Tsukuba-4 (P. persica), Tsukuba-5 (P. persica), Nanking cherry (P. tomentosa) and wild peach (P. persica). The susceptible tomato (Lycopersicon esculentum Mill.) cultivar ‘Baiguoqiangfeng’ was used as a positive control. Results. Nematodes did not penetrate roots of Tsukuba-4 and Tsukuba-5, which were considered to be immune varieties. Nanking cherry was highly resistant to M. incognita, whereas wild peach was susceptible. Conclusion. The differences in resistance among the rootstocks were not attributed to differences in effects of root diffusates, but were related to the different structural organizations of the root tips. The epidermal structure of Tsukuba-4 and Tsukuba-5 completely prevented the penetration of second-stage juveniles of M. incognita (J2). In Nanking cherry, penetration of J2 juveniles was reduced, and the development of nematodes from the J2 to female stage was delayed.

Introduction. Algerian oasesare characterized by a diversification of local fruit species andcultivars. However, this diversity is subject to erosion. To preservethe genetic heritage, we undertook the first work on the inventoryof fruit species crops in the region of Touggourt in south-easternAlgeria. Materials and methods. Our working methodswere based on surveys carried out on farms in the Oued Righ regionaccording to a questionnaire that met our objectives. For this,we divided the study region into three zones and in each zone weselected ten farms for the inventory of fruit species cultivated. Results and discussion. The results of our investigationsallowed us to identify thirteen species, including four specieswith a wide distribution (pomegranate tree, apricot tree, fig treeand vine). The local genetic diversity of these species is littleknown. The varieties or cultivars inventoried have local names givenby the farmers in the region according to certain morphologicalcharacteristics of the fruit. We surveyed five cultivars for thefig tree, two cultivars for the pomegranate tree, two cultivarsfor the vine, and two apricot tree cultivars. The study of the biodiversityof fruit trees in the Oued Righ region showed high richness of thespecies and cultivars; some are local and others are endemic tothe region. Better knowledge of genetic diversity will begin withtests of behavior and characterization of the different genotypes encounteredto dispel the ambiguities and to highlight the actual compositionof populations or varieties of different species.

Abstract – Introduction. The susceptibility of banana fruit to crown rot andanthracnose, the two main banana post-harvest diseases, is influenced by many pre-harvestabiotic factors. Mycosphaerella leaf spot diseases (MLSD) of bananas arebiotic pre-harvest factors, which have an influence on fruit physiology. The fruit’ssusceptibility to post-harvest diseases may also be influenced by foliar diseases causedby M. fijiensis, responsible for black leaf streak disease (BLSD), andM. musicola, which causes sigatoka disease (SD). The aim of our studywas to determine the influence of these biotic pre-harvest factors on banana fruit’ssusceptibility to crown rot and anthracnose. Materials and methods. A diseaseseverity gradient was established in two experimental fields (Cameroon for BLSD andGuadeloupe for SD) where, at the flowering stage, six different levels of MLSD severitywere selected. Fruit susceptibility was determined through necrotic surface assessmentsafter artificial inoculation by Colletotrichum musae on the 3rd hand ofharvested bunches. Results and discussion. BLSD significantly influencedbanana sensitivity to crown rot (P < 0.001) but only had a slighteffect on the development of anthracnose ( P = 0.041). SD had no effect(P > 0.05) on banana susceptibility to either post-harvest disease.These results are discussed with emphasis on the influence of variations in thesource-sink ratio on fruit physiology. The influence of BLSD on crown rot disease suggeststhe need to take into account the management of these foliar diseases for an alternativecontrol method of post-harvest diseases through integrated pest management programs.

Introduction. The fruit and vegetable sector group of the French National Institute for Agricultural Research (INRA) undertook a bibliometric analysis of INRA scientific academic literature on fruits and vegetables for the 2000–2006 period. This study made it possible to produce quantitative and qualitative indicators that will shed light on the institute's scientific position in relation to the fruit and vegetable sectors. Materials and methods. Different steps were taken to constitute and process a corpus of INRA researcher publication data. The citation corpus gathering bibliographic data was constituted using the Web of Science. The Sphinx data processing program allowed the constituted databases to be structured and manipulated. Results. After the data were cleaned, there was a total of 1463 articles published by INRA authors between 2000 and 2006; this constituted the “fruit and vegetable corpus” studied, which represented 8% of the total number of INRA publications during this period. The typology of fruits and vegetables studied at INRA, the INRA research centres involved, the main subjects addressed by INRA research divisions and the national and international partnerships of INRA authors were analysed. Conclusion. The analysis presented highlights the overall magnitude of research dedicated to fruits and vegetables at INRA. This research is undertaken in the principal research centres of horticulture and/or fruit regions, with a high level of involvement of Southeast centres of France. Partnerships with other French and foreign organisations are numerous. This assessment allows an understanding of the rapid evolution of research that has occurred over the past several years in the fruit and vegetable field, notably the growing involvement of nutrition and consumer sciences. The development of multi-disciplinary and integrated approaches must also be emphasised.

Introduction. En Tunisie, bien que des problèmes liés à la floraison et à la fructification se posent chez le grenadier (Punica granatum L.), aucune étude n'avait encore été menée sur cette espèce. Nos travaux ont donc cherché à étudier ces importantes étapes du cycle de reproduction de la plante chez certaines variétés locales de P. granatum exploitées dans les conditions tunisiennes. Matériel et méthodes. La dynamique de la floraison a été étudiée par le comptage régulier des fleurs formées sur branches des trois cultivars locaux : Gabsi, Kalaii et Garoussi. La chute des boutons floraux, des fleurs épanouies ou des jeunes fruits récemment noués a été comptabilisée pour l'arbre entier. Un suivi de la fertilité des fleurs et des essais d'autopollinisation par ensachage de rameaux de différentes longueurs ont été conduits sur le seul cultivar Gabsi. Résultats et discussion. La durée de la floraison a été de 8 semaines environ pour Gabsi et de 10 semaines pour Garoussi et Kalaii. Le maximum des fleurs a été obtenu entre la troisième et la cinquième semaine après le début de floraison. La majeure partie des fleurs est apparue sur du bois âgé d'un an ou plus. Le taux de rétention le plus élevé (8,4 %) a été enregistré chez le cultivar Gabsi. Les fruits ont surtout été obtenus à partir des fleurs hermaphrodites. Le calibre des fruits à la récolte a diminué au fur et à mesure que la date d'apparition des fleurs dont ils provenaient avait été tardive. Les rameaux courts ensachés n'ont pas retenu de fruits ; ceux de longueur moyenne ont permis un taux de nouaison de 2,3 % par rapport à la totalité des fleurs du rameau ; les rameaux de plus grande taille ont présenté un taux de 4,8 %. Conclusions. La floraison du grenadier est très échelonnée. Les cultivars étudiés ont différé en fonction de la date du début de floraison, de sa durée et de son intensité. Le régime de reproduction s'est révélé être l'allogamie préférentielle. L'étalement de la floraison et les particularités morpho-physiologiques des fleurs favoriseraient plutôt la pollinisation croisée.

Introduction. Biennial bearing and malformation are the two present problems of mango which are threatening the very existence of a global mango industry in India. Many factors are closely associated with these problems but, presently, those really responsible are not known exactly. Polyphenol oxidase (PPO) plays vital roles in higher plants and, hence, its activity was measured in swollen buds at panicle initiation in regular and biennial bearing mango cultivars to establish a relation between the PPO activity and the flowering behaviour or the malformation incidence. Materials and methods. Six regular (Sensation, Eldon, Tommy Atkins, Amrapali, Neelum and Bangalora) and six biennial bearing cultivars (Langra, Chausa, Edward, St. Alexandrina, Extrema and Bombay Green) were studied. PPO activity was measured in fully swollen buds by preparing a crude enzyme extract at 4 °C. Catecholase activity was measured by using 4-methyl catechol (4-MC) as the substrate, while 4-methyl phenol (p-cresol) was used for cresolase. Results and discussion. Both catecholase and cresolase activities were higher in the swollen buds in the year 2000 than in 1999. Regular bearers exhibited low PPO activity and showed the higher incidence of malformation as compared to the biennial bearing cultivars.Whatever their type of production, cultivars exhibited significant variations in PPO activities and malformation incidence. Conclusion. A strong negative correlation between PPO activity and number of panicles, and PPO activity and malformation incidence, suggests that the PPO activity is inversely related to both flowering and malformation incidence in mango.

Introduction. Le safoutier (Dacryodes edulis, Burseraceae) est un arbre dont les fruits sont très appréciés par les populations de son aire de répartition. Plante allogame, la multiplication par semis ne permet pas d'effectuer une multiplication conforme de l'arbre sélectionné. La présente étude a eu pour principal objectif de mettre au point une technique de régénération in vitro des bourgeons axillaires et apex prélevés sur de jeunes plants issus de la germination de graines, afin d'adapter ultérieurement cette technique au clonage d'arbres adultes par utilisation de repousses formées après recépage. Matériel et méthodes. Des nœuds et apex prélevés sur des plants de Dacryodes edulis issus de la germination de graines ont été mis en culture in vitro sur un milieu Murashige et Skoog dilué de moitié (MS 1/2). Les effets du charbon actif et de la benzyleadéninepurine (BAP) sur le débourrement des explants ont été étudiés, de même que l'influence de l'acide naphtalènacétique (ANA) et de la photopériode sur l'enracinement. Résultats. Le milieu MS 1/2 additionné de charbon actif à 2g × L-1 et de BAP à 2,22 µM ou 4,44 µM a provoqué le meilleur débourrement des bourgeons axillaires et une bonne croissance des tigelles. L'ANA à 5,37 µM a favorisé l'enracinement. Une période de 7 j à l'obscurité s'est révélée favorable à l'induction racinaire. Deux types de racines ont été observés : les racines néoformées (issues de cals) et les racines adventives. L'acclimatation a été faite avec succès sur substrat constitué de tourbe et vermiculite. La régénération des vitroplants à partir des nœuds et apex permet de distinguer le long de la jeune plante un gradient morphogénétique acropétale. Discussion et conclusion. Ces résultats pourront être exploités pour la mise au point d'une micropropagation conforme à partir de jeunes repousses formées après la taille d'un safoutier présentant des caractéristiques intéressantes.

Effects of peach or hybrid rootstocks on growth and cropping of two cultivars of peach trees (Emeraude and Zephyr).Abstract - Introduction. Rootstock is an important consideration for a productive peach orchard, especially in a replanting situation. A peach and two hybrid rootstocks, grafted with an early and a late-season peach cultivar, were planted and their growth compared. The experimental area had been used since 1970, for two generations of successive peach crops. Materials and methods. Emeraude (early-season) and Zephyr (late-season) were grafted onto GF 305 (peach rootstock), GF 677 and Cadaman$^{\circledR}$ Avimag (hybrid rootstocks), and were planted in a split-plot experimental design. Fruit yield and vegetative growth were assessed annually. Results. Vegetative growth was greater with the hybrid rootstocks than with the peach rootstock. Fruit production was higher with Cadaman than with GF 677, which was higher than with GF 305. Yield differences associated with rootstocks were greater with Emeraude in comparison with Zephyr, as suggested by the rootstock × cultivar interaction. Discussion. Compared to the previous peach plantation on the same land, the differences observed in this current experiment, between the peach rootstock GF 305 and the hybrid rootstock GF 677, widened, suggesting a replanting decline with the peach rootstock. In the given conditions, the hybrid rootstock Cadaman appeared to be better suited than GF 677 to improving peach production.

Introduction. Planting time is considered as one of the most important factors for successful strawberry cultivation. Traditionally, under north-Indian plains, strawberry is planted after the second week of October, which results in the availability of fruits for a month or so. With the use of plasticulture techniques, the planting time can be enhanced, which facilitates early and higher production, and enhances availability of the fruit for a longer period. Mulching is the most important cultural practice in strawberry, which influences plant growth, fruit yield and quality. Hence, studies were conducted to determine the influence of planting time and mulching on growth, flowering and fruiting behaviour, yield and quality of strawberry in northern plains of India. Materials and methods. Studies were conducted on ‘Chandler’ strawberry with three planting times (mid-September, mid-October and mid-November) as the main effect and three mulching materials (black polyethylene, clear polyethylene and paddy straw) as the sub-main effect in a split-plot design with three replications. Standard procedures were adopted for making observations on plant growth parameters, flowering and fruiting behaviour, yield and quality parameters under different treatment combinations. Results and discussion. Mid-September planting favoured vigorous growth, enhanced flowering and fruiting, which resulted in the production of the largest fruits and highest yield of the best quality. Among the three different mulch materials, plants mulched with black polyethylene had the best growth, fruit weight, yield and quality compared with those mulched with clear polyethylene or paddy straw mulch. The [planting time × mulching] interaction was also found to be significant for all measured parameters. Conclusion. Our study revealed that, in northern plains of India, to get an early and high yield of the best quality under a micro-irrigation system, Chandler strawberry could be planted in mid-September if mulched with black polyethylene.

Introduction. In Cameroon, most subsistence farmers apply a mixed cropping system, resulting in a range of possible nematode host plants. Bananas and plantains (Musa spp.) are an important component of these cropping systems, both as a source of cash income and as a staple food. In order to program future extension projects, our study analyzed the cropping associations and their constraints in the West and Northwest provinces of Cameroon highlands. As nematodes have been identified as a major constraint to Musa production worldwide, we tried to assess the nematode presence. Materials and methods. A survey of over 200 households was done throughout the Cameroon highlands to identify the types of crop associations and preferences, management practices and pest awareness of small-scale farmers. In addition, samples were taken to examine the prevalence of Pratylenchus goodeyi on bananas and plantains in the home garden and one field of each household visited. Results and discussion. Cropping systems were highly variable between households, in so far as they could not easily be typified. Thirty-eight crops were identified planted in association with Musa spp. Bananas and plantains ranked as the most important crop for 43% of the farmers, followed by the staple crop maize (19%) and the traditional cash crop coffee (12%). Pest awareness (% awareness) was relatively high with respect to weevils (72%). The major constraints as perceived by the farmers related to leaf necrosis, weevils and nematode damage. Nevertheless, only 15% of them had heard of a nematode before. All root samples revealed nematode presence (over 40% showed more than 104 individuals·100 g–1 root fresh weight). P. goodeyi was the dominant species found.

Introduction. Les réactions d’oxydation constatées régulièrement dans les aliments peuvent causer des dommages importants, entraînant parfois des cancers et maladies chroniques. Les extraits d’huiles essentielles étant connus pour posséder d’importantes propriétés thérapeutiques, notre étude a cherché à comparer les activités antiradicalaires et anti-inflammatoires des essences de C. reticulata var. Madagascar et C. sinensis var. Casagrande. Matériel et méthodes. Les huiles essentielles des feuilles d’agrumes ont été extraites par hydrodistillation et leur composition chimique a été analysée par chromatographie en phase gazeuse et par chromatographie en phase gazeuse couplée à la spectrométrie de masse. La détermination des propriétés antiradicalaires a été faite par la méthode du piégeage des radicaux libres à l’aide du diphenyl picryl hydrazyl (DPPH). En revanche, la méthode enzymatique par la lipoxygénase a permis d’évaluer l’activité anti-inflammatoire des extraits d’huiles essentielles obtenues par hydrodistillation. Résultats. Les propriétés antiradicalaires de nos échantillons ont été évaluées avec une SC50 égale à (4,10 ± 0,05) g·L–1 et (7,40 ± 0,55) g·L–1, respectivement, pour C. reticulata var. Madagascar et C. sinensis var. Casagrande, alors que le composé antiradicalaire de référence (butylhydroxytoluène ou BHT) donnait une SC50 de (7,02 ± 0,5) mg·L–1. Par ailleurs, les deux échantillons ont montré une activité anti-inflammatoire exploitable (IC50 de 53,5 mg·L–1 et 20,3 mg·L–1, respectivement) même si le NDGA (acide nordihydroguaiarétique) a eu une IC50 de 0,7 mg·L–1. Conclusion. C. reticulata var. Madagascar se révèle avoir une activité antiradicalaire plus importante que C. sinensis var. Casagrande, alors que cette dernière espèce a présenté l’activité anti-inflammatoire la plus marquée. Ces huiles essentielles pourraient être utilisées comme additifs alimentaires.

Introduction. This protocol aims at preparing total RNA for gene expression analysis by Northern blots, RT-PCR and real-time quantitative PCR; cDNA isolation by RT-PCR; and cDNA library construction. The principle, key advantages, starting plant material, time required for obtaining total RNA and expected results are presented. Materials and methods. This part describes the required materials and the 27 steps necessary for preparing RNA from peel and pulp fruit tissue: preparation of plant tissue powder, preparation of the complete RNA extraction buffer and isolation of RNA from ground banana fruit tissue. Results. Extraction of total RNA by the method described makes it possible to achieve electrophoresis under denatured conditions and in vitro reverse transcription. An example for Northern blot analysis is illustrated.