Sixteen castrated male Large White × Landrace pigs were employed to investigate the muscle type-specific changes of gene expression in response to recombinant porcine growth hormone (rpGH) administration. Pigs were injected intramuscularly with rpGH (4 mg/day, n = 8) or saline (n = 8) for 28 days. Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to determine the mRNA abundance of genes related to muscle growth in longissimus dorsi (LD) and semitendinosus (ST) muscles. Myofibre-type composition was characterised by the ratio of the expression of myosin heavy chain (MyHC) 1, 2a or 2b relative to 2x. The results showed that the relative myofibre-type composition of neither LD nor ST was affected by rpGH administration. rpGH administration did not induce significant changes in the abundances of myostatin and myogenin mRNA in both types of muscle. MyoD and calpain 3 mRNA were significantly increased after rpGH treatment in ST muscle, whereas the difference was not significant in LD muscle. A tendency of down-regulation was observed for PGC-1α mRNA expression in ST muscle of rpGH-treated group (P = 0.16). These results suggest that myoD, calpain 3 and probably PGC-1α may be involved in the mechanism of exogenous GH action on skeletal muscle growth; rpGH up-regulates mRNA expression of myoD and calpain 3 in a muscle type-specific manner, being more remarkable in ST than in LD, whereas no influences of rpGH on the mRNA expression of myostatin and myogenin were detected.

In vitro gas production kinetics of six different substrates, pectin (PEC), xylan (XYL), starch (STA), cellulose (CEL), commercial compound feed (FEED; 201 g crude protein per kg, 155 g crude fibre per kg, 334 g neutral-detergent fibre (NDF) per kg and 190 g acid-detergent fibre (ADF) per kg) and an NDF prepared from commercial compound feed (NDFFEED) were determined using the caecum contents of weaned rabbits (36 days of age) and of rabbits at slaughter age (78 days of age) as inoculums. The cumulated gas production over 96 h of incubation was modelled with Gompertz model, and the kinetic parameters compared. The total potential gas production (parameter ‘B’ of the Gompertz model) was not affected (P>0.05) by the inoculum source, except with STA, where rabbits at slaughter weight had significantly higher total potential fermentability (314 ml/g dry matter (DM)) than those at weaning age (189 ml/g DM). Intensities of fermentation (maximum fermentation rate; MFR) of PEC (32.2 ml/h) and XYL (24.4 ml/h) were significantly greater in rabbits at weaning, while that of STA (45 ml/h) was significantly lower than at slaughter age (23.0, 14.3 and 14.0 ml/h for PEC, XYL and STA, respectively). The MFRs of CEL and NDFFEED were very similar between inoculum sources. In the first 10 h of fermentation which correspond to the normal retention time of the substrates in the caecum, the highest amount of gas was produced from PEC, followed by FEED and XYL. These substrates had a time of maximum fermentation rate (TMFR) at both rabbit ages short enough (8.0 and 9.5 h for PEC, 9.5 and 6.6 h for FEED, 13.7 and 14.2 h for XYL at weaning and at slaughter age, respectively) to be almost completely fermented in vivo.

The role of dietary fat during early pregnancy in sows has not yet been fully established. The aim of the study was to determine the consequences of altering the fatty acid profile of sow diets during the first half of gestation; oils of different fatty acid composition were chosen as energy supplements to provide diets with different fatty acid profiles. A group of 48 multiparous sows were used to evaluate the effects of supplemental feeding during the first 60 days of gestation (term ≈ 115 days). Sows were allocated (eight per treatment) to either 3 kg/day of commercial sow pellets (control; C) or an experimental diet consisting of 3 kg/day of commercial sow pellets supplemented with 10% extra energy in the form of excess pellets (E), palm oil (P), olive oil (O), sunflower oil (S) or fish oil (F). Differential effects were observed with respect to the fatty acid profile of the diet during the first half of gestation. P sows gave birth to the largest litters. Both P and O supplementation of the maternal diet resulted in heavier piglets at birth, after correction for differences in litter size. P piglets possessed the most fat at birth and remained fatter throughout the pre-weaning period; in contrast, the offspring of O sows contained the least fat throughout life (0 to 140 days of age). The offspring of F sows exhibited improved growth performance during the neonatal period. In conclusion, altering the fatty acid profile of sow diets during the first half of gestation has long-term consequences for the growth and development of their offspring.

Urea production may be impaired in intrauterine growth restriction (IUGR), increasing the risk of toxic hyperammonaemia after birth. Arginine supplementation stimulates urea production, but its effects in IUGR are unknown. We aimed to determine the effects of IUGR and arginine supplementation on urea production and arginine metabolism in the ovine foetus. Pregnant ewes and their foetuses were catheterised at 110 days of gestation and randomly assigned to control or IUGR groups. IUGR was induced by placental embolisation. At days 120 and 126 of gestation, foetal urea production was determined from [14C]-urea kinetics and arginine metabolism was determined from the appearance of radioactive metabolites from [3H]-arginine, both at baseline and in response to arginine or an isonitrogenous mixed amino acid supplementation. Urea production decreased with gestational age in the embolised animals (13.9 ±  3.1 to 11.2 ±  3.0 μmol/kg per min, P ≤ 0.05) but not in the controls (13.3 ±  3.5 to 14.8 ±  6.0 μmol/kg per min). Arginine supplementation increased urea production in both groups, but only at 126 days of gestation (control: 15.0 ±  8.5 to 17.0 ±  9.4 μmol/kg per min; embolised: 11.7 ±  3.1 to 14.3 ±  3.1 μmol/kg per min, P ≤ 0.05). Embolisation reduced foetal arginine concentrations by 20% ( P ≤ 0.05) while foetal arginine consumption was reduced by 27% ( P ≤ 0.05). The proportions of plasma citrulline and hydroxyproline derived from arginine were reduced in the embolised animals. These data suggest that foetal urea production and arginine metabolism are perturbed in late gestation after placental embolisation.

Dietary fat is well recognised as an important macronutrient that has major effects on growth, development and health of all animals including humans. The amount and type of fat in the diet impacts on many aspects of metabolism including lipoprotein pathways, lipid synthesis and oxidation, adipocyte differentiation and cholesterol metabolism. It has become increasingly apparent that many of these effects may be due to direct modulation of expression of key genes through the interaction of fatty acids with certain transcription factors. Peroxisome proliferator-activated receptors (PPARs), the liver X receptors (LXRs), hepatic nuclear factor 4 (HNF-4) and sterol regulatory binding proteins (SREBPs) represent four such factors. This review focuses on emerging evidence that the activity of these transcription factors are regulated by fatty acids and the interactions between them may be responsible for many of the effects of fatty acids on metabolism and the development of chronic disease.

Six 34-kg barrows were fitted with a post-valve T-caecum cannula and assigned to six dietary treatments according to a 6 × 5 change-over design to study how a mixture of formic acid, sorbate, and benzoate (0 or 8.4 g/kg feed) influences apparent ileal and faecal digestibility coefficients, bacterial nitrogen (N) flow, microbial metabolite concentrations, and passage rate in pigs fed isoenergetic diets with medium, high, or very high fibre content (neutral-detergent fibre (NDF): 199, 224, and 248 g/kg dry matter, respectively). These barley and soya-bean meal based diets contained 0, 75, and 150 g/kg barley fibre (NDF: 577 g/kg) and 0, 8, and 16 g/kg rapeseed oil, respectively. The dietary organic acid mixture improved the apparent ileal digestibility of 14 of the 17 amino acids analysed (P < 0.05). Increasing levels of dietary fibre linearly decreased the apparent ileal digestibility of six of the 17 amino acids analysed (P < 0.05). Ileal flows of bacterial N and amino acids as assessed on the basis of purine flow were decreased by the dietary organic acid mixture (P < 0.05) but were not affected by dietary fibre level (P>0.05). As assessed on the basis of diaminopimelic acid flow, bacterial N flow was increased by both the dietary organic acid mixture and increased dietary fibre levels (P < 0.05). The dietary organic acid mixture reduced the concentration of lactic acid and increased that of acetic acid in ileal digesta (P < 0.05), while dietary fibre levels had a quadratic effect on concentrations of acetic, propionic, and butyric acid (P < 0.05). The mean retention time of Co (solute marker) and Yb (particle marker) in the large intestine decreased in a linear manner by increasing dietary fibre levels (P < 0.05) but was not affected by the dietary organic acid mixture (P>0.05). The results show that a dietary organic acid mixture has a positive effect on the apparent ileal digestibility of most amino acids irrespective of dietary fibre levels. This could be at least partly related to changes in bacterial N flow in the ileum. However, different bacterial markers showed opposite effects on bacterial N flow, which makes it questionable to use a constant bacterial marker / bacterial N ratio to estimate bacterial N flow. Increasing levels of dietary fibre had negative effects on the apparent ileal amino acid digestibilities and shortened the mean retention time of digesta in the large intestine.

The objectives of this study were to see if the body condition score curve during lactation could be described using a model amenable to biological interpretation, a non-linear function assuming exponential rates of change in body condition with time, and to quantify the effect of breed and parity on curves of body condition during lactation. Three breeds were represented: Danish Holstein (n = 112), Danish Red (n = 97) and Jerseys (n = 8). Cows entered the experiment at the start of first lactation and were studied during consecutive lactations (average number of lactations 2, minimum 1, maximum 3). They remained on the same dietary treatment throughout. Body condition was scored to the nearest half unit on the Danish scale (see Kristensen (1986); derived from the Lowman et al. (1976) system) from 1 to 5 on days: 2, 14, 28, 42, 56, 84, 112, 168, 224 after calving. Additionally, condition score was recorded on the day of drying off the cow, 35, 21, and 7 days before expected calving and finally on the day of calving. All condition scores were made by the trained personal on the research farm, where the same person made 92% of the scores. The temporal patterns in condition score were modelled as consisting of two underlying processes, one related to days from calving, referred to as lactation only, the other to days from (subsequent) conception, referred to as pregnancy. Both processes were assumed to be exponential functions of time. Each process was modelled separately using exponential functions, i.e. one model for lactation only and one for pregnancy, and then a combined model for both lactation only and pregnancy was fitted. The data set contained 467 lactation periods and 378 pregnancy periods. The temporal patterns in condition score of cows kept under stable and sufficient nutritional conditions were successfully described using a two component non-linear function. First lactation cows had shallower curves, they had greater condition scores at the nadir of the curve. Danish Holstein and Jersey were thinner at the end of the mobilisation period having lost more body condition than the Danish Red breed. Although the dairy breeds ended up being thinner there were no significant differences in the rate at which they lost body condition.

Muscle lipid concentration is known to influence pork eating quality. This study aimed at evaluating the effect of a restriction–re-alimentation feeding strategy on intramuscular fat deposition in pigs. A total of 70 Duroc × (Large White × Landrace) pigs (castrated males and females) were used. Ten pigs were first slaughtered at 30 kg live weight (LW) to determine initial body and muscle composition. From 30 to 80 kg LW (growing period), pigs were either fed ad libitum (AL) or restricted to 70% of the ad libitum intake of AL pigs (RA). From 80 to 110 kg LW (finishing period), both AL and RA pigs were fed ad libitum. In each group, pigs were slaughtered at 80 kg (n = 10) and at 110 kg (n = 20) LW. During the growing period, the growth rate of RA pigs was reduced by 30% (P < 0.001) compared with AL pigs. During the finishing period, RA pigs had a 7% (P = 0.09) higher growth rate than AL pigs due to compensatory feed intake (+14%). Plasma insulin-like growth factor-1 concentration was lower in RA pigs at 80 kg LW, but markedly increased after re-alimentation up to the level observed in AL pigs (P < 0.001). At 80 kg, the leaner carcasses of RA pigs resulted from a more pronounced reduction in fat than in lean tissue deposition rates. Re-alimentation of RA pigs increased fat tissue deposition (+160% for females, P < 0.01) but not lean deposition in the carcass, leading to limited differences in carcass composition between RA and AL pigs at 110 kg LW. Regarding tissue deposition rates, the response to feeding strategy differs between muscles. In the m. biceps femoris (BF), restriction affected lipid (−50%, P < 0.001) and protein (−25%, P < 0.001) deposition, whereas re-alimentation increased lipid (+62%, P < 0.05) but not protein deposition rates. At market weight, the extent of the difference in BF lipid concentration between RA and AL pigs was strongly reduced, but still significant. By contrast, in the m. longissimus, restriction decreased protein but not lipid deposition, whereas neither of them was modified during re-alimentation. Therefore, an increased muscle lipid concentration at 110 kg LW could not be reached in RA pigs. Modifications of onset and/or duration of restriction and re-alimentation periods should be tested to optimise effects on muscle lipid deposition and thereby achieve improved pork quality.

Predictive frameworks for performance under both physical and social stressors are available, but no general framework yet exists for predicting the performance of animals exposed to pathogens. The aim of this paper was to identify the key problems that would need to be solved to achieve this. Challenges of a range of hosts by a range of pathogens were reviewed to consider reductions in growth beyond those associated with reductions in voluntary food intake (VFI). Pair-feeding and marginal response studies identified the extent and mechanisms of how further reductions in growth occur beyond those caused by reduced VFI. Further reductions in growth depended on the pathogen, the host and the dose and were time dependent. In some instances the reduction in VFI fully explained the reduction in growth. Marginal response experiments showed increased maintenance requirements during exposure to pathogens, but these were different for specific amino acids. There were no clear effects on marginal efficiency. Innate immune functions, repair of damaged tissue and expression of acquired immunity caused significant but variable increases in protein (amino acid) requirements. More resistant genotypes had greater requirements for mounting immune responses. The partitioning of protein (amino acids) was found to be different during pathogen challenges. Prediction of the requirements and partitioning of amino acids between growth and immune functions appears to be a crucial problem to solve in order to predict performance during pathogen challenges of different kinds and doses. The problems of accounting for reductions in performance during pathogen challenges that are described here provide a useful starting point for future modelling and experimental solutions.

The whole-animal model described in this paper is intended to be a research model with an intermediary structure between sophisticated and simple cow models. The mechanistic model structure integrates the main metabolic pathways of the lactating dairy cow. Milk yield and related feed intake for varying production potentials were considered to be the driving forces and were empirically defined. The model was designed to explain the main metabolic flows and variations in body reserves associated with the push of nutrients from dry matter intake and their pull by the mammary gland to synthesise milk components throughout lactation. The digestive part of the model uses either known feed unit systems (e.g. PDI system for protein) or published empirical equations for the prediction of digestive flows of organic matter, starch and fatty acids and ruminal volatile fatty acid production. The metabolic sub-system is made up of four tissue compartments (body protein, body lipid, protein in the uterus and triglycerides in the liver) and five circulating metabolites (glucose, amino acids, acetate + butyrate, propionate, and triglycerides and non-esterified fatty acids). A major original aspect of the model is its homeorhetic control system. It was assumed that flows relating to adipose and protein tissue anabolism and catabolism were driven by a couple of linked theoretical hormones controlling mobilisation and anabolism. The evolution in body composition and body weight were outcomes of this control. Another originality of the model is its suitability for milk yields varying from 10 to 50 kg at peak production. This was achieved by homeorhetic control of milk potential acting on several key metabolic flows. Homeostatic regulation was also introduced in order to confine the behaviour of the model within realistic physiological values. For some basic aspects (e.g. Uterine involution, standard kinetics of body protein and fat), it was necessary to build specific databases from the literature and interpret these by meta-analysis. Fairly realistic simulated kinetics were obtained for body composition, liver triglycerides, blood plasma metabolite concentrations, milk protein and fat contents, and also for major groups of milk fatty acids.

This study aimed to determine the consequences of altering the fatty acid profile of sow diets during mid-to-late gestation; oils of different fatty acid composition were chosen as energy supplements to provide diets with different fatty acid profiles. Forty-eight multiparous sows were used to evaluate the effects of fat supplementation from day 60 of gestation until parturition. Sows were allocated to either 3 kg/day of commercial sow pellets (control; C) or an experimental diet consisting of 3 kg/day of commercial sow pellets supplemented with 10% extra energy in the form of excess pellets (E), palm oil (P), olive oil (O), sunflower oil (S) or fish oil (F). From days 0 to 60 of gestation, all sows were given 3 kg/day of sow pellets as for the C group. The E diet resulted in the heaviest piglets at birth whereas the offspring of O and S sows were the lightest at birth. The offspring of S sows remained lighter throughout the pre-weaning period, and were also the leanest by 14 days of age. In contrast, pigs born to S sows possessed more fat by the time they reached commercial end point (≈140 days of age). In conclusion, altering the fatty acid profile of the sow diet during the second half of gestation has long-term consequences for the development of their offspring.

In seasonal goats and sheep breeds, onset of puberty is modified by the season of birth. As adult does and bucks from subtropical Mexico display seasonal variation in their reproductive behaviour, this study was carried out to determine the effect of season of birth on puberty. Three groups of each sex born in January, May and October were used. During the seasons, does and bucks were weaned at an age of 30 days and offered ad libitum alfalfa hay and 100 g of commercial concentrate. In the female kids, the onset of ovulatory activity was determined by progesterone plasma concentrations once in a week from 3 months of age until the onset of puberty. In the male kids, the onset of puberty was individually recorded by observing the ability to mount and intromit an induced oestrous female goat aged 3 months and the presence of spermatozoa in the ejaculate obtained in an artificial vagina 1 week after the first mount. In female kids, there was an effect of the season on the date of first ovulation (P < 0.001). In the May group, ovulatory activity commenced at an earlier age (201 ± 3 days) compared with January (264 ± 5 days) and October (344 ± 5 days) groups (P < 0.001). In the January group also, the ovulatory activity commenced earlier than the October group (P < 0.001). In males, an effect of the season of birth on the first mounting was observed (P < 0.001). The male kids that were born in May (111 ± 3) and October (112 ± 5 days) attained puberty earlier than those born in January (131 ± 4 days; P < 0.001). The time of onset of puberty did not differ between groups of May and October. All males showed the presence of spermatozoa in the first ejaculate obtained 1 week after the first mount. The spermatozoa in all ejaculates were immobile. It was concluded that the season of birth modified the onset of puberty in both genders, but these modifications were more pronounced in the female than in the male kid goats.

Rabbit lung is often used to study the pathophysiology of some congenital anomalies affecting the lung because its development is very comparable with that of human. Retinoids and the molecular transducers of the retinoic signal play a crucial role in mammalian lung development. In the rabbit, the molecular retinoic pathway has so far been poorly studied. As a first step in elucidating this process, we aimed to identify the retinoic acid receptors (RARs) and retinoid X receptors (RXRs). We cloned a part of the nuclear receptors (RARs and RXRs), and we used reverse transcription - polymerase chain reaction (RT-PCR) and immunohistochemistry assays to demonstrate the presence of RAR (α,β) and RXR (α, β) at all stages of rabbit lung development. Our results initiate further analysis into the molecular and genetic functions of retinoids during normal and pathological rabbit lung development including the surgical model of congenital diaphragmatic hernia.

The present study was designed to identify the volatile constituents across the oestrous cycle of bovine in order to detect oestrous-specific chemical signal. The bovine saliva was extracted with diethyl ether (1 : 1 ratio, v/v) and analysed by gas chromatography-linked mass spectrometry. Numerous compounds were identified during oestrous cycle of bovine saliva. Among these, the compounds, namely, trimethylamine, acetic acid, phenol 4-propyl, pentanoic acid and propionic acid were specific to oestrous stage. The behaviour assay revealed that the compound, trimethylamine, is involved in attracting the male animal. The result concludes that the trimethylamine is considered as a putative oestrous-specific salivary chemo-signal in the bovine.

Routinely performed painful procedures are of increasing interest and, in 2001 (Royal Order, May 17), Belgium prohibited docking in several vertebrates including horses. In 2004, opponents to this decision submitted a Bill (Doc51 0969/001) to Parliament, intending to obtain derogation for Belgian draught horses, which were traditionally docked. The Animal Welfare Council of Belgium, an official body advising the Minister of Public Health, was asked to evaluate this complex question, including biological, ethical and socio-economic aspects, on the basis of the available peer-reviewed studies. In this context, this study reviews legal aspects (overview of the European legislation), zootechnic aspects (uses of the Belgian draught horse) and biological aspects (pain potentially related to docking; horses’ welfare linked to insect harassment and hygiene, communication and reproduction) of tail docking in draught horses. We conclude that (1) there is no benefit for horses in tail docking, including Belgian draught horses, (2) potential advantages of docking are essentially in favour of humans and these advantages could be scrupulously re-evaluated, taking into account practices of other countries. Therefore, there is no need to dock any horse other than for veterinary reasons.

It is well established that dietary protein supply can influence the peri-parturient breakdown of immunity to nematode parasites but there is no information on the importance of exposure to nematode larvae during pregnancy for this response. We investigated this by exposing housed pregnant sheep, scanned as carrying two lambs, to a vaccinating infection with a trickle mixed infection of Teladorsagia circumcincta and Trichostrongylus colubriformis larvae (L3) or to no infection during weeks − 9 to − 4 relative to parturition. At the beginning of week − 3 all sheep were treated with anthelmintic to remove any vaccinating worm burden and from week − 2 to week +6 received a trickle challenge infection with the same nematodes. Within each vaccinating treatment there were two nutritional treatments (no. = 20 per subgroup) designed to provide 1.5 or 1.0 and 1.3 or 0.8 of metabolisable protein (MP) requirement during pregnancy and lactation, respectively. Five ewes were necropsied during weeks +1 and +3 to measure worm burdens and mucosal inflammatory cells and the remainder maintained until week +6. Serum levels of total, IgA and IgE antibodies against L3 antigen of each nematode were measured.

Scanning errors and lamb losses resulted in some ewes carrying and/or rearing only one lamb. Numbers of lambs reared was therefore introduced as a treatment effect. Vaccinating infection delayed the peri-parturient rise in faecal egg count (FEC) by an average of 2 weeks but its effect on FEC during the first 6 weeks of lactation was smaller and less persistent than that of dietary MP supply and single- v. twin-suckling.

Populations of both nematodes were lower in association with high MP supply, vaccination and single suckling. These changes were associated with increases in numbers of mucosal mast cells (MMC) as a result of both increased MP supply and vaccination. Evidence for a more rapid return of host ability to limit populations of the abdominal nematode T. circumcincta than of the intestinal nematode T. colubriformis was associated with fewer eosinophils and more globule leucocytes (GL) in abomasal than in intestinal tissue.

None of the serum antibody isotypes was affected by dietary protein supply. Total and IgA antibodies were maintained by a current larval (vaccinating) intake. IgA titres, however, increased progressively during pregnancy, especially in twin-bearing ewes. IgE titres appeared to be sensitive primarily to the reproductive cycle itself, peaking around parturition.

This work supports the conclusion that availability of MP supply influences the recruitment and activity of cells of the immune armoury of the gastro-intestinal tract to nematode parasites. The precise outcome may differ with site and/or nematode species.

Appropriate thyroid gland function and thyroid hormone activity are considered crucial to sustain the productive performance in domestic animals (growth, milk or hair fibre production). Changes of blood thyroid hormone concentrations are an indirect measure of the changes in thyroid gland activity and circulating thyroid hormones can be considered as indicators of the metabolic and nutritional status of the animals. Thyroid hormones play a pivotal role in the mechanisms permitting the animals to live and breed in the surrounding environment. Variations in hormone bioactivity allow the animals to adapt their metabolic balance to different environmental conditions, changes in nutrient requirements and availability, and to homeorhetic changes during different physiological stages. This is particularly important in the free-ranging and grazing animals, such as traditionally reared small ruminants, whose main physiological functions (feed intake, reproduction, hair growth) are markedly seasonal. Many investigations dealt with the involvement of thyroid hormones in the expression of endogenous seasonal rhythms, such as reproduction and hair growth cycles in fibre-producing (wool, mohair, cashmere) sheep and goats. Important knowledge about the pattern of thyroid hormone metabolism and their role in ontogenetic development has been obtained from studies in the ovine foetus and in the newborn. Many endogenous (breed, age, gender, physiological state) and environmental factors (climate, season, with a primary role of nutrition) are able to affect thyroid activity and hormone concentrations in blood, acting at the level of hypothalamus, pituitary and/or thyroid gland, as well as on peripheral monodeiodination. Knowledge on such topics mirror physiological changes and possibly allows the monitoring and manipulation of thyroid physiology, in order to improve animal health, welfare and production.

Prediction of nutrient partitioning is a long-standing problem of animal nutrition that has still not been solved. Another substantial problem for nutritional science is how to incorporate genetic differences into nutritional models. These two problems are linked as their biological basis lies in the relative priorities of different life functions (growth, reproduction, health, etc.) and how they change both through time and in response to genetic selection. This paper presents recent developments in describing this biological basis and evidence in support of the concepts involved as they relate to nutrient partitioning. There is ample evidence that at different stages of the reproductive cycle various metabolic pathways, such as lipolysis and lipogenesis, are up or down regulated. The net result of such changes is that nutrients are channelled to differing extents to different organs, life functions and end-products. This occurs not as a homeostatic function of changing nutritional environment but rather as a homeorhetic function caused by the changing expression of genes for processes such as milk production through time. In other words, the animal has genetic drives and there is an aspect of nutrient partitioning that is genetically driven. Evidence for genetic drives other than milk production is available and is discussed. Genetic drives for other life functions than just milk imply that nutrient partitioning will change through lactation and according to genotype – i.e. it cannot be predicted from feed properties alone. Progress in describing genetic drives and homeorhetic controls is reviewed. There is currently a lack of good genetic measures of physiological parameters. The unprecedented level of detail and amounts of data generated by the advent of microarray biotechnology and the fields of genomics, proteomics, etc. should in the long-term provide the necessary information to make the link between genetic drives and metabolism. However, gene expression, protein synthesis etc, have all been shown to be environmentally sensitive. Thus, a major challenge in realising the potential afforded by this new technology is to be able to be able to distinguish genetically driven and environmentally driven effects on expression. To do this we need a better understanding of the basis for the interactions between genotypes and environments. The biological limitations of traditional evaluation of genotype ×  environment interactions and plasticity are discussed and the benefits of considering these in terms of trade-offs between life functions is put forward. Trade-offs place partitioning explicitly at the centre of the resource allocation problem and allow consideration of the effects of management and selection on multiple traits and on nutrient partitioning.

In many cases health and welfare problems are observed in organic egg production systems, as are high environmental risks related to nutrient leaching. These disadvantages might be reduced if the layers are allowed to utilise their ability to forage to a higher degree thereby reducing the import of nutrients into the system and stimulating the hens to perform a natural behaviour. However, very little is known about the ability of modern high-producing layers to take advantage of foraging to cover their nutritional needs, and the aim of the present work was to clarify this subject. Six flocks, each of 26 hens and one cock, were moved regularly in a rotation between different forage crops for a period of 130 days. Half of the flocks were fed typical layer feed for organic layers and half were fed whole wheat. The forage crops consisted of grass/clover, pea/vetch/oats, lupin and quinoa. At the beginning of the experiment, wheat-fed hens had a lower intake of supplementary feed (wheat) and a lower laying rate, egg weight and body weight. However, after a period of 6 to 7 weeks, the intake of wheat increased to approximately 100 g per hen per day and the laying rate increased to the same level as for the hens fed layer feed. For both groups of hens egg weight and body weight increased during the remaining part of the experiment. Crop analysis revealed different food preferences for hens fed layer feed and wheat-fed hens. Wheat-fed hens ate less of the cultivated seeds, whereas the amounts of plant material, oyster shells, insoluble grit stone and soil were larger in the crops from wheat-fed hens. Floor eggs were significantly more frequent in the hens fed layer feed, whereas wheat-fed hens only rarely laid floor eggs. Irrespective of treatment, hens were found to have excellent health and welfare. We conclude that nutrient-restricted, high-producing organic layers are capable of finding and utilising considerable amounts of different feed items from a cultivated foraging area without negative effects on their health and welfare.

Inappropriate floors in pig pens and slippery floor conditions may cause leg problems that reduce animal welfare. Therefore the objectives of the present study were to characterise the walk of pigs on dry concrete solid floor, to evaluate whether pigs modify their gait according to floor condition, and to suggest a coefficient of friction (COF) that ensures safe walking on solid concrete floors for pigs. Kinematic (50 Hz video recordings in the sagittal plane) and kinetic (1 KHz force plate measuring three perpendicular ground reaction forces) data were collected from four strides of both the fore- and hindlimbs of 30 healthy pigs walking on dry, greasy and wet concrete floor with 10 pigs on each floor condition. The COF of the floor conditions were tested in a drag-test. The results from the gait analysis showed that the pigs adapted their gait to potentially slippery floors by lowering their walking speed and reducing their peak utilised COF on greasy and wet (contaminated) floors compared with dry floors. Moreover, the pigs shortened their progression length and prolonged their stance phase duration on greasy floor compared with dry and wet floors. Thus the greasy floor appeared the most slippery condition to the pigs, whereas the wet floor was intermediate to the other two conditions. The pigs walked with a four-beat gait, and the limbs differed biomechanically, as the forelimbs carried more load, received higher peak vertical forces and had longer lasting stance phases than did the hindlimbs. The utilised COF from the gait analysis indicated that a high floor COF (>0.63) is needed to prevent pigs from slipping and thus to ensure safe walking on dry concrete floors.