One of the early changes upon tuber induction is the switch from apoplastic to symplastic unloading. Whether and how this change in unloading mode contributes to sink strength has remained unclear. In addition, developing tubers also change from energy to storage-based sucrose metabolism. Here, we investigated the coordination between changes in unloading mode and sucrose metabolism and their relative role in tuber sink strength by looking into callose and sucrose metabolism gene expression combined with a model of apoplastic and symplastic unloading. Gene expression analysis suggests that callose deposition in tubers is decreased by lower callose synthase expression. Furthermore, changes in callose and sucrose metabolism are strongly correlated, indicating a well-coordinated developmental switch. Modelling indicates that symplastic unloading is not the most efficient unloading mode per se. Instead, it is the concurrent metabolic switch that provides the physiological conditions necessary to potentiate symplastic transport and thereby enhance tuber sink strength .

Potato producers in Canada’s Atlantic provinces of Prince Edward Island (PE) and New Brunswick rely on photosystem II (PSII)-inhibiting herbicides to provide season-long weed control. Despite this fact, a high proportion of common lambsquarters populations in the region have been identified as resistant to this class of herbicides. Crop-topping is a late-season weed management practice that exploits the height differential between weeds and a developing crop canopy. Two field experiments were conducted in Harrington, PE, in 2020 and 2021, one each to evaluate the efficacy of a different crop-topping strategy, above-canopy mowing or wick-applied glyphosate, at two potato phenological stages, on common lambsquarters viable seed production and potato yield and quality. Mowing common lambsquarters postflowering decreased viable seed production (72% to 91%) in 2020 but increased seed production (78% to 278%) in 2021. Mowing had minimal impact on potato marketable yield across cultivars in both years. In contrast, treating common lambsquarters with wick-applied glyphosate had variable impacts on seed output in 2020 but dramatically reduced seed production (up to 95%) in 2021 when treatments were applied preflowering. Glyphosate damage to potato tubers was not influenced by timing and resulted in a 14% to 15% increase in culled tubers due to black spotting and rot. Our results highlight the importance of potato and common lambsquarters phenology when selecting a crop-topping strategy and demonstrate that above-canopy mowing and wick-applied glyphosate can be utilized for seedbank management of herbicide-resistant common lambsquarters in potato production systems.

Potato is the third most important staple food crop globally following rice and wheat. In the United States, potato is grown on approximately 410,000 ha with a farm-gate value of US$1,032 million. In Canada, potato is grown on approximately 134,000 ha with a farm-gate value of US$235 million. The objective of this manuscript, compiled by the Weed Science Society of America Weed Loss Committee, was to estimate potato yield loss caused by weed interference. Potato yield data from weedy and weed-free plots (or plots with >95% weed control) was obtained from researchers working on weed management in potato in the United States and Canada or from published manuscripts from 2000 to 2018. Potato yield loss from weed interference was 12% to 61% when no weed management tactics were implemented. The average yield loss for all states/provinces (where data was obtained) due to weed interference was 44%. Weed interference would cause a farm-gate loss of approximately US$465 million and US$61 in the United States and Canada, respectively, if weeds are not controlled. These results indicate that weed management is critical for successful potato production, and that an ongoing need for research exists on weed management in this crop.

Myzus persicae (Sulzer) is an important agricultural pest worldwide causing major economic losses due to its ability to transmit over 100 viruses including Potato virus Y (PVY). Myzus persicae shows considerable variation with respect to performance on its host plants. The objective of this study was to use a survival experiment, behavioural observations, including observations of probing and feeding behaviour obtained using the electrical penetration graph (EPG) technique, and a PVY acquisition experiment to determine whether or not potato was still the more suitable host for M. persicae originating on potato and reared on a novel host, table beet, for over 15 years. In a survival experiment, the pre-reproductive period was significantly longer while adult survival and whole longevity were significantly lower for M. persicae reared on beet fed beet leaves compared to M. persicae reared on potato fed potato leaves. The number of progenies produced and fecundity were both significantly reduced (90 and 85%, respectively) for M. persicae reared on beet fed beet leaves. Ethological observations and EPG assessment of M. persicae behaviour reared on beet placed on beet leaves showed significantly impaired behavioural responses compared to M. persicae reared on potato placed on potato leaves. The rate of PVY acquisition was the same for M. persicae reared on beet and on potato. These results indicate that after 15 years on table beet, M. persicae still performs better on its original host, potato, and appears to be a specialized potato-adapted genotype.

Field trials were conducted to determine the effects of glyphosate and/or dicamba simulated drift rates on chipping potatoes (Solanum tuberosum L.) ‘Atlantic’ and ‘Dakota Pearl.’ Sublethal herbicide rates were applied at the tuber initiation stage and consisted of dicamba at 99 g ae ha−1 or glyphosate at 197 g ae ha−1 applied alone or the combinations of dicamba at 20 or 99 g ae ha−1 and glyphosate at 40 or 197 g ae ha−1, respectively. At 7 days after treatment (DAT), the high spray combination of glyphosate plus dicamba resulted in the greatest plant damage (28%). Plant injury from plants treated with the low combination of glyphosate plus dicamba did not differ from the nontreated control. At 21 DAT, visible injury increased to 40% for plants treated with the high combination of glyphosate plus dicamba. Total yield suggested that dicamba and glyphosate caused similar yield reductions as plants that received glyphosate at 197 g ha−1 or dicamba at 99 g ha−1 had lower total yields compared to the nontreated and plants that received the combination of glyphosate (197 g ha−1) and dicamba (99 g ha−1) had lower total yields compared to plants that received either herbicide alone. However, ‘Dakota Pearl’ plants were more sensitive to glyphosate at 197 g ha−1 than were ‘Atlantic’ plants, causing the interaction for most tuber grades. Tuber specific gravity was lower for plants that received glyphosate at 197 g ha−1, dicamba at 99 g ha−1, or this combination, but this reduction would not prevent chip processing. Results reinforce the need for diligence when applying these herbicides in proximity to a susceptible crop, such as chipping potatoes, and the need to thoroughly clean sprayers before application to a sensitive crop.

The ability to use the protoporphyrinogen oxidase (PPO)-inhibiting herbicides fomesafen, flumioxazin, and sulfentrazone in potato is limited regionally or by soil texture, largely because of crop injury noted in research in the 1990s. With that in mind, we evaluated whether reducing the herbicide rates could maintain weed control while providing more consistent crop safety. Studies were conducted on a silt loam and a coarse-textured loamy sand soil. Soil texture played a greater than anticipated role in PPO-inhibitor herbicide injury risk as it relates to high-precipitation events. For example, in 2020 at the silt loam location, there were five precipitation events across the season that exceeded 2.5 cm, including one 6 d after treatment (DAT), and a seasonal total precipitation that was over 10 cm greater than the previous year. Despite excessive moisture and initial potato injury as high as 27% where flumioxazin was applied at the high rate with S-metolachlor, by 29 DAT injury was less than 10% in all treatments, and marketable tuber yield was similar among treatments. In contrast, in 2020 at the loamy sand location, there were four precipitation events across the season that exceeded 2.5 cm, and potato injury was as much as 60%. In 2020 the high amount of injury from flumioxazin was hypothesized to be caused by precipitation before herbicide application and not after, suggesting a need for more research in this area. This work documents the fine line between yield reduction presumably caused by reduced weed control and yield reduction assumed to be related to herbicide injury. This delineation between adequate weed control and consistent crop safety may differ by soil texture and environmental conditions, supporting the notion that custom-tailored weed management may become more necessary as high-precipitation events become more common in upper midwestern U.S. agricultural systems.

Field studies were conducted from 2005 to 2009 in Idaho and Oregon to 1) evaluate the competitive effect of volunteer potato on sugar beet yield (volunteer potato competition experiment), and 2) determine the optimum timing of volunteer potato removal from glyphosate-tolerant sugar beet fields using glyphosate (volunteer potato removal timing experiment). The volunteer potato competition experiment consisted of eight potato densities, including the untreated check: 0, 6,741, 10,092, 13,455, 16,818, 20,184, 26,910, and 40,365 tubers ha−1. The volunteer potato removal experiment consisted of 10 removal timings (including the untreated check) ranging from the 10-cm rosette stage to mid-tuber bulking. There was a linear decrease in sugar beet root and sucrose yield as volunteer potato density increased (P < 0.001) such that with every volunteer potato tuber per square meter, sugar beet root yield decreased by 15% and sucrose yield decreased by 14%. At the highest volunteer potato density (40,365 tubers ha−1), sugar beet root yield was 29,600 kg ha−1 (compared to 73,600 kg ha−1 for the untreated), representing a 60% reduction in sugar beet root yield. In the removal timing study, a one-time application of glyphosate at the 10-cm rosette, hooking, and tuber initiation stages provided 74% to 98% reduction in volunteer potato tuber biomass. Delaying volunteer potato removal beyond the tuber initiation stage reduced sugar beet root and sucrose yield (12% to 20%), resulting in an economic loss of $104 to $161 per hectare. The best potato removal timing that optimizes the trade-off between improved control and potential for sugar beet yield reductions is before or at the tuber initiation stage.

The potato tuber moth, Phthorimaea operculella (Zeller), is a serious pest of potato and other commercial crops belonging to the Solanaceae family. In recent years, it has become an emerging problem in potato-growing regions of the Nilgiri hills of southern India. It is responsible for the reduced quality and quantity of marketable potatoes. In this regard, the development of an eco-friendly control method for the management of the potato tuber moth is urgently required. Therefore, in the present study, the virulence of Steinernema cholashanense CPRSUS01 originally isolated from the potato rhizosphere was tested on fourth-instar larvae and pupae of P. operculella. Steinernema cholashanense caused the greatest mortality in the fourth-instar larval stage (100%) than the pupae (30%). In addition to this, penetration and reproduction of this nematode was also studied in fourth-instar larvae of P. operculella and this is the first report of penetration and reproduction of any entomopathogenic nematode species on potato tuber moth larvae. The reproduction capacity of S. cholashanense on P. operculella is higher (702 infective juveniles mg−1 body weight). Our results indicated that S. cholashanense has good potential as an alternative tool for the management of P. operculella. But before including S. cholashanense in the integrated pest management program of P. operculella, its efficacy should be tested under field conditions.

Nosema leptinotarsae Lipa, 1968 is a microsporidian pathogen of the Colorado potato beetle, Leptinotarsa decemlineata Say. (Coleoptera: Chrysomelidae). To determine the phylogenetic status of N. leptinotarsae, the 16S SSU rRNA gene was sequenced (GenBank Accession No. MN841279) and compared phylogenetically against 21 microsporidian 16S SSU rRNA sequences using neighbour-joining and maximum-parsimony methods. The per cent identities of the N. leptinotarsae and other members of the Nosema–Vairimorpha clade ranged from 78.1 to 98.5%. Pairwise phylogenetic distances between the N. leptinotarsae and other species ranged from 0.009 to 0.320. Phylogenetic analysis shows clearly that N. leptinotarsae is a member of the Vairimorpha clade rather than the Nosema clade. The sequence divergence and morphological traits separated the N. leptinotarsae from other species in the Vairimorpha complex. As a result, a new assignment of Vairimorpha leptinotarsae comb. nov. has been implemented for N. leptinotarsae according to the phylogenetical positioning in the present study.

Analysis of the potato yield gap and the corresponding nutrient gap can help in devising strategies and measurements to increase productivity for closing the gaps through improved practices. On-farm experiments conducted in the main potato production areas of northwest China were used to determine attainable yield. Official statistical data were used to determine the actual on-farm yield. Yield gap was the difference between attainable yield and actual on-farm yield. Nutrient gap was calculated by dividing the size of yield gap by partial factor productivity. Results indicated that nitrogen (N), phosphorus (P) and potassium (K) fertilization increased potato yield by an average of 1169–7625, 2937–5336 and 2331–7338 kg/ha, respectively. The maximum attainable yields (the 90th percentile yields) were 50 145, 37 855, 30 261 and 56 616 kg/ha and the average actual on-farm yield were 14 179, 16 732, 10 271 and 19 990 kg/ha in the Inner Mongolia Autonomous Region (IMAR), Gansu, Ningxia and Qinghai provinces, respectively. In the above four regions, yield would need to increase by 165, 70, 112 and 121% from actual yield to reach 75% of attainable yield. Compared with recent 3-year average NPK rates by farmers, the total NPK rates need to increase by 90.1–134.3% for IMAR, 42.9–69.2% for Gansu, 68.1–111.2% for Ningxia and 48.1–83.8% for Qinghai to improve productivity to near the 75% attainable yield. In conclusion, the high yield responses to fertilizer application provide opportunities to close the large yield gaps through balanced nutrition.

Quinclorac was registered for weed control in wheat (Triticum spp.) for western Canada in 1997. Residues from quinclorac may persist in the soil and may damage following crops; therefore, field and growth chamber experiments were conducted to determine the tolerance of several following rotational crops. Cereals and bromegrass (Bromus biebersteinii) were sufficiently tolerant that they could be seeded within 16 d of quinclorac application without risk of injury. At the other extreme, marketable and total potato (Solanum tuberosum) yields were reduced by quinclorac on irrigated land 1 yr after application. Growth chamber experiments were used to rank crops in order of their tolerance of quinclorac residues and to compare the tolerance of crops that were grown in the field with additional crops. Quinclorac injured several legume and oilseed crops when the crops were seeded immediately after application, but quinclorac did not reduce the dry matter yield of two of the most sensitive legumes, faba bean (Vicia faba) and alfalfa (Medicago sativa), 1 yr after application on irrigated land. However, based on a previous study, one can conclude that injury to these crops may occur in the field under drought conditions in rain-fed agricultural systems.

Winter rape (Brassica napus L.) green manures have shown potential for erosion control and suppression of weeds and other pests in potato cropping systems. However, little information on residue cover, biomass production, glucosinolate concentration, and glucosinolate production with winter rape grown as a green manure is available. In field trials in southern Idaho, ‘Aspen,’ ‘Bridger,’ ‘Cascade,’ ‘Dwarf Essex,’ and ‘Humus’ winter rape were planted in mid-August and incorporated the following spring in late April or early May. All five cultivars provided > 80% fall, winter, and early spring residue cover. Winter rape dry weight just before incorporation was 2,880 to 4,462 kg/ha in 1994 and 5,438 to 7,837 kg/ha in 1995. The major glucosinolate in roots of all five cultivars was phenylethyl glucosinolate; the major glucosinolates in shoots were 4-pentenyl, 2-hydroxybutenyl, 3-butenyl, and 2-hydroxypentenyl glucosinolate. Glucosinolate concentrations varied between years, but concentrations were higher in Dwarf Essex and Humus than in Aspen both years. Glucosinolate production per hectare also was highest in Dwarf Essex and Humus and lowest in Aspen. Dwarf Essex and Humus produced higher amounts of isothiocyanate (ITC) and oxazolidinethione (OZT)-producing glucosinolates than Aspen, Bridger, or Cascade, and therefore may be better biofumigants.

Having knowledge on genetic similarity and DNA profile of potato genotypes facilitates a breeder's decision for parent selection and provides accurate variety identification. Fingerprinting and identification of genetic similarity among 151 potato genotypes were achieved using an inter-primer-binding sites (iPBS) retrotransposon marker system. Our study is the first application of iPBS markers for fingerprinting and distinguishing large numbers of Solanum tuberosum genotypes. Initially, 16 potato genotypes were screened using 45 iPBS retrotransposon markers to identify polymorphisms. Seventeen of these primers were selected for fingerprinting the whole set of accessions due to strong, reproducible and polymorphic bands. The 17 iPBS primers produced 290 scorable bands of which 224 were polymorphic. The number of bands per primer ranged from 10 to 26 with an average of 17.1. The number of polymorphic bands per primer was between 6 and 21. The polymorphism percentage per primer ranged from 46.2 to 100.0% with an average of 77.2% per primer. The mean polymorphism information content (PIC) values of iPBS primers varied from 0.12 to 0.31 per primer. Genetic similarity based on Jaccard's coefficient of potato genotypes ranged from 0.61 to 0.93 with an average of 0.73. The data produced herein may be used for selection of appropriate parents and variety description in the future. The findings of the present study suggest that iPBS retrotransposons are powerful and easy DNA markers for fingerprinting the large samples of potato germplasm.

Field studies were conducted in 2010 in Ontario, OR, to evaluate the response of direct-seeded dry bulb onion, sugar beet, and pinto beans to imazosulfuron soil residues 12 mo after application to control weeds in potato. The studies were organized as randomized complete block designs with three replications each. Imazosulfuron was applied alone PRE at 224 and 450 g ai ha−1, sequentially at 224 g ha−1 PRE and POST, or in tank mixture with S-metolachlor 1,060 g ha−1. Very few onion plants emerged in plots previously treated with imazosulfuron at 224 g ha−1, regardless of timing. Emerged onion plants were severely injured and never matured. No onions emerged from residues of imazosulfuron applied at 450 g ha−1. A few sugar beet plants emerged from 224 g ha−1 but were severely stunted and never grew beyond the first set of leaves. There was no sugar beet emergence from imazosulfuron sequential applications, regardless of the rate and application timing. However, imazosulfuron residues did not affect pinto beans, which emerged and produced marketable yield, similar to grower standard and nontreated plots. The results suggest sensitivity of direct-seeded dry bulb onion and sugar beet, but not pinto beans, to imazosulfuron residues 12 mo after application.

A field study was conducted at Vezaiciai branch of the Lithuanian Institute of Agriculture from 1998 to 2000 to determine weed emergence dynamics in potato and to estimate the effect of different intervals of weed crop competition on potato tuber yield if regular interrow cultivation was applied. Treatments varying in intervals of weed-free conditions and competition were laid out in a randomized complete block design with six replications. The greatest emergence of annual broadleaf such as common lambsquarters and other weeds (62 to 86% of the season total) in the crop was observed in the period from potato planting until flowering. Emergence of winter annuals such as field violet and scentless mayweed was greatest in the period from the 20-cm potato plant height until harvesting. The 20-cm potato height was the most important stage affecting potato yield loss due to weed competition. Potato yield losses due to weed competition were minimized when weeds were removed before the potatoes reached 20 cm or kept clean from this point forward. The results indicated that the critical weed-free period, when weed competition was detrimental to yield, started from planting and lasted until 25 d after flowering.

Field trials were conducted to determine potato response to parts per trillion (ppt) per weight concentrations of sulfometuron in soil. The herbicide was applied to achieve targeted, 0-d soil concentrations of 0, 7.5, 15, 30, 60, 120, 240, 480, and 960 ppt. Russet Burbank potatoes were planted immediately after application using standard agronomic practices. Based on midseason visual evaluations, root and tuber injury occurred with 0-d concentrations of only 7.5 ppt. Concentrations at or above 120 ppt caused a significant increase in number of tubers with deformities compared with the control. By the end of the growing season, 0-d concentrations between 120 and 240 ppt resulted in higher percentages of tubers with deformities, such as cracks, knobs, or folds. Using logistic models fit to U.S. No. 1 tuber yield and net return data, doses of 74, 156, and 324 ppt are predicted to result in 5, 10, and 20% U.S. No. 1 yield reductions, respectively. The model predicted a 20% net return loss, approximately $160/ha, occurring at 262 ppt, which is near the 240 ppt concentration determined by standard ANOVAs and means comparisons with single degree of freedom contrasts causing significant tuber quality and yield reductions in our study. Growers using the 240 ppt concentration as an indicator of a no-effect level would encounter actual losses too great to withstand. This modeling approach provides an initial attempt at giving growers the tools necessary for assessing potential losses.

Field studies were conducted to determine if POST applications of the p-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitors mesotrione (105 g ai/ha), topramezone (18 g ai/ha), and tembotrione (92 g ai/ha) applied alone and in mixtures with the photosystem-II (PSII) inhibitors atrazine (560 g ai/ha), bentazon (560 g ai/ha), or bromoxynil (280 g ai/ha) would control volunteer potato. Mesotrione alone controlled potato 62%, but topramezone and tembotrione only provided 10 to 22% control by 6 wk after treatment (WAT). All PSII inhibitors applied alone provided less than 36% control of potato. Overall, mixtures of PSII inhibitors plus mesotrione improved initial potato control by 2 WAT; however, by 6 WAT, few differences were observed between mesotrione applied alone or in mixtures with PSII inhibitors. PSII inhibitors did not always improve activity of topramezone or tembotrione on potato, and in some instances appeared to antagonize control. HPPD inhibitors applied alone or in combinations with PSII inhibitors reduced potato yields in comparison to the untreated check.

Potato producers rely heavily on herbicides for the majority of weed control. However, recent occurrences of herbicide-resistant weed populations and the lack of new herbicide registrations have stimulated interest in alternative strategies. The choice of potato cultivars that can suppress or tolerate weed competition could be a component of an integrated weed management system to reduce reliance on herbicides. The competitive ability of 10 potato cultivars—‘Atlantic’, ‘Bannock Russet’, ‘Dark Red Norland’, ‘Goldrush’, ‘Rodeo’, ‘Russet Burbank’, ‘Russet Norkotah’, ‘Snowden’, ‘Superior’, and ‘Villetta Rose’—was evaluated in 2006 and 2007 in Hancock, WI. Weed competition treatments included (1) weedy throughout the season, (2) weed-free from emergence to 4 wk after emergence (WAE) by hand-weeding, and (3) weed-free by hand-weeding for the entire season. Potato cultivars did not differ in ability to reduce weed biomass. Early-season time of potato emergence and canopy closure, as well as weed competition treatments, were strongly related to potato tuber yield. In general, Bannock Russet yield relative to weed-free controls of the same cultivar was less than that of most other cultivars. Overall, Atlantic, Russet Burbank, Snowden, and Superior yields (relative to weed-free control yields) usually were greater than the yields of other cultivars under weedy conditions. Although the ability to suppress weeds was similar among cultivars, differences in yield among cultivars grown in the presence of weeds suggest differential tolerances of weed competition.

We demonstrate how direct (constrained) ordination methods can be used to analyze differential control by different weed management tactics. Weed biomass data of individual weed species were compiled for 110 trials on potatoes, each of which included a mechanical control treatment, a herbicide treatment, and an untreated control. We used “trial id” as 110 categorical dummy covariables, thereby eliminating between-trial variation, and made direct comparisons between the data from numerous trials. Unweeded plots had the largest biomass of all species as expected. Contrasting the results for the standard herbicide and the mechanical control treatments in a separate analysis, we could rank species from those best controlled by the herbicide (several annuals) to those best controlled by the mechanical treatment (a perennial grass and a climbing annual).