In this study, for the first time Bacillus methylotrophicus Y37 cellulase was purified and recovered in a single step by three-phase partitioning (TPP). The optimal purification parameters for TPP were 40% ammonium sulfate saturation (m/v) with a 1.0:1.0 (v/v) ratio of crude extract:t-butanol, which gave 5.8-fold purification with 155% recovery of cellulase. Non-covalent immobilization of the partitioned cellulase was performed using bentonite as a support material. The activity observed in the 20th experiment was 100%. The optimal pH values and temperatures determined for the free enzyme and the immobilized enzyme were 5.0 and 6.0 and 45°C and 50°C, respectively. The Arrhenius activation energy (Ea) of the immobilized enzyme was lower than that of the free enzyme, whereas the Michaelis–Menten constant (Km) and maximum velocity (Vm) of the immobilized enzyme increased. The turnover number (kcat) and the catalytic performance (kcat/Km) demonstrated the improved catalytic properties of the immobilized enzyme compared to the free enzyme. Immobilization of cellulase is thermodynamically preferred.

A Pickering emulsion was prepared via synergistic stabilization of a lipase and palygorskite particles. The optimum conditions for the stabilization of the Pickering emulsion, such as the concentrations of the palygorskite particles and lipase, were explored. The morphology of emulsion droplets was examined using digital optical microscopy and polarizing optical microscopy. The palygorskite–lipase co-stabilized Pickering emulsions were investigated by determination of the adsorption rate, pH and zeta potential of the aqueous dispersion, as well as by determining the contact angle values of the lipase solution on a palygorskite disc that was immersed in toluene. The catalytic performance of the immobilized lipase in the Pickering emulsion was studied via the investigation of its thermal stability, storage stability and reusability. The immobilized lipase showed greater stability than the free lipase. The lipase immobilized by Pickering emulsion retained a high level of activity even after seven periods of recycling.

Conservation agriculture (CA) and no-till (NT)-based cropping systems could address soil degradation and fertility decline in southern Africa. A multi-location and multi-year experiment was carried out between 2008 and 2014 to assess the effects of different levels of maize residue biomass (0, 2, 4, 6 and 8 t ha−1) and nitrogen (N) fertilizer (0, 30, 90 kg ha−1) on maize performance under no-tillage. In some sites, different (N) fertilizer levels were superimposed to test their effects on maize grain yield and leaf chlorophyll content under different maize residue biomass levels. The different residue levels had no significant effect on maize yield in most growing seasons. Maize residue cover increased grain yield in eight out of 39 site-years across the sites used. However, in some sites, maize yield decreased with increases in residue level in cropping seasons that had average to above average rainfall. At a few sites maize yield increased with increase in residue level. Seasonal rainfall pattern influenced the effect of different residue levels on grain yield at most sites. Nitrogen fertilizer increased maize yield regardless of the residue level applied. This study demonstrates that mulching with maize residues in CA/NT systems results in limited maize yield gains – at least within the first 6 years in different agro-ecological conditions of southern Africa.

Pyrolized carbon in biochar can sequester atmospheric CO2 into soil to reduce impacts of anthropogenic CO2 emissions. When estimating the stability of biochar, degradation of biochar carbon, mobility of degradation products, and ingress of carbon from other sources must all be considered. In a previous study we tracked degradation in biochars produced from radiocarbon-free wood and subjected to different physico-chemical treatments over three years in a rainforest soil. Following completion of the field trial, we report here a series of in-vitro incubations of the degraded biochars to determine CO2 efflux rates, 14C concentration and δ13C values in CO2 to quantify the contributions of biochar carbon and other sources of carbon to the CO2 efflux. The 14C concentration in CO2 showed that microbial degradation led to respiration of CO2 sourced from indigenous biochar carbon (≈0.5–1.4 μmoles CO2/g biochar C/day) along with a component of carbon closely associated with the biochars but derived from the local environment. Correlations between 14C concentration, δ13C values and Ca abundance indicated that Ca2+ availability was an important determinant of the loss of biochar carbon.

Lactoperoxidase (LPO) is an antimicrobial protein present in milk that plays an important role in natural defence mechanisms during neonatal and adult life. The antimicrobial activity of LPO has been commercially adapted for increasing the shelf life of dairy products. Immobilization of LPO on silver nanoparticles (AgNPs) is a promising way to enhance the antimicrobial activity of LPO. In the current study, LPO was immobilized on AgNPs to form LPO/AgNP conjugate. The immobilized LPO/AgNP conjugate was characterized by various biophysical techniques. The enhanced antibacterial activity of the conjugate was tested against E. coli in culture at 2 h intervals for 10 h. The results showed successful synthesis of spherical AgNPs. LPO was immobilized on AgNPs with agglomerate sizes averaging approximately 50 nm. The immobilized conjugate exhibited stronger antibacterial activity against E. coli in comparison to free LPO. This study may help in increasing the efficiency of lactoperoxidase system and will assist in identifying novel avenues to enhance the stability and antimicrobial function of LPO system in dairy and other industries.

Three samples of terra rossa were shown to be efficient adsorbents of phosphate [P(V)] from wastewater and removed 29.9–32.6% of P(V). The total iron content in terra rossa was the key factor which determined the P(V) removal from wastewater. The original samples of terra rossa were effective support materials for the immobilization of metabolically active P(V)-accumulating bacteria Acinetobacter junii (0.56–2.47×1010 CFU g–1). The removal of oxalate-extractable iron from original sample of terra rossa increased the number of immobilized bacteria to 1.34×10–11 CFU g–1, which is the largest number of immobilized bacteria reported in the literature so far. In reactors containing the A. junii and terra rossa P(V) was removed from wastewater by simultaneous adsorption onto terra rossa and accumulation inside bacterial cells, resulting in 40.5–62.5% of P(V) removal. Terra rossa is a promising substrate for biological P(V) removal from wastewater, acting both as adsorbent of P(V) and carrier of P(V)-accumulating bacteria.

Buckwheat residues can suppress both emergence and growth of weeds, but the mechanisms of this suppression are not well understood. The main objectives of this research were to evaluate the possible role of (1) low initial nitrogen (N) availability and (2) fungal pathogens in this suppression for three sensitive weed species: Powell amaranth, shepherd's-purse, and corn chamomile. Growth chamber experiments were conducted comparing weed emergence and growth in bare soil or soil with freshly incorporated buckwheat residue at multiple rates of N fertilization with or without fungicide seed treatment. In the absence of N or fungicide addition, emergence of all weed species was reduced 40 to 70%, and dry weight was reduced 85% in buckwheat residue compared with bare soil. For all three weed species, suppression of growth by buckwheat residue was completely overcome with the addition of N. For shepherd's-purse and corn chamomile (2005 only), suppression of emergence was also overcome with the addition of N. In 2006, treatment of corn chamomile seeds with fungicide resulted in a higher emergence in buckwheat residue than in bare soil. In contrast, suppression of Powell amaranth emergence was not overcome with N fertilization or fungicide treatment. The results suggest that buckwheat-mediated changes in N dynamics account entirely for suppression of weed growth but that the mechanisms responsible for suppression of emergence by buckwheat residue vary by species. Fungal and N effects account for suppression of emergence of corn chamomile and shepherd's-purse, but the mechanism of suppression for Powell amaranth remains obscure.

Introduction: The Canadian Prehospital Evidence-based Practice (PEP) project is an online, freely accessible, continuously updated EMS evidence repository. The summary of research evidence for EMS interventions used to care for blunt spinal trauma is described. Methods: PubMed was systematically searched. One author reviewed titles and abstracts for relevance. Included studies were scored by trained appraisers on a three-point Level of Evidence (LOE) scale (based on study design and quality) and three-point Direction of Evidence (DOE) scale (supportive, neutral, or opposing results). Second party appraisal was conducted for included studies. Interventions were plotted on a 3x3 table (DOE × LOE) for the spinal injury condition based on appraisal scores. The primary outcome was identified for each study and categorized. Results: Seventy-seven studies were included. Evidence for adult and paediatric blunt spinal trauma interventions was: supportive-high quality (n=1, 7 %), supportive-moderate quality (n=3, 21.4%), supportive-low quality (n=1, 7%), neutral-high quality (n=1, 7%), neutral-moderate quality (n=5, 35.7%), neutral-low quality (n=1, 7%), opposing-high quality (n=0, 0%), opposing-moderate quality (n=0, 0%), opposing-low quality (n=1, 7%). One (7%) intervention had no evidence. Interventions with supportive evidence were: steroids, cervical-spine clearance, scoop stretcher, self-extrication and “leaving helmet in place”. The evidence weakly opposed use of short extrication devices. Leading study primary outcomes were spinal motion, diagnostic accuracy, and pressure/discomfort. Conclusion: EMS blunt spinal trauma interventions are informed by moderate quality supportive and neutral evidence. Future research should focus on high quality studies filling identified evidence gaps using patient-oriented outcomes to best inform EMS care of blunt spinal injury.

Introduction: Following a protocol derived from the Canadian C-spine Rule (CCR), patients 65 years and older transported by ambulance after trauma require full spinal immobilisation. Immobilisation complicates the transport and the evaluation; potential side effects have been recognized. The aim of this study was to evaluate the effect of mechanism of trauma and age on the rate of cervical injury in a geriatric population. Methods: We conducted a retrospective observational study on patients 65 years and older transported by ambulance to a level-one trauma center from March 2008 to October 2013. The outcome was the rate of clinically important cervical spine injury (CICSI), defined as any fracture, dislocation or ligamentous injury needing treatment or specialised follow up. The rate was calculated in the geriatric population and in the subgroup of patients with minor trauma, defined as a fall from a standing height, a chair or a bed. We then looked at the rate of CICSI based on age to define a subgroup at lower risk of lesion. Results: We included 1221 patients with a mean age of 80 y.o. (SD = 8), 739 women (61%). CICSI was found in 53 patients (4.3%, 95% CI 3.2-5.4). This is similar to the rate found in patients 65 years and older in the NEXUS population (4.6%) and the CCR population (6.0%). The mechanism of injury was a minor trauma for 716 patients (59%). Of those, 24 patients (3.4%, 95% CI 2.1-4.7) had CICSI. The rate increased after 85 y.o in both the overall population (3.4% vs 6.4%) and the minor trauma subgroup (2.6% vs 4.4%). Conclusion: The subgroup of patients 65-84 y.o. with a minor trauma had the lower rate of cervical spine injury (2.6%). In a lot of prehospital systems, those patients are not systematically immobilised for transport. It will be interesting to review the files of all patients with CICSI to identify any possible case that would have been missed without the age criteria.

Background: A randomised study to investigate whether bilateral arm abduction is superior to unilateral abduction with respect to stability using shifts on treatment as the main outcome measure.

Material and Methods: 50 consecutive female patients were randomised to ipsilateral or bilateral arm abduction. Central lung depth (CLD) and cranial caudal depth (CCD) on the simulator image was compared with that featured on three Electronic Portal Images (EPI) captured during treatment for each patient. Systematic and random errors were analysed with respect to the average translational displacement and standard deviation per patient and per population between the planning image and the EPI.

Results: The CLD average translational displacement in the test group was −1.7 mm (95% CI = −5 to 1.6 mm) Σ pop = 2.3 mm, σ pop = 1.6 mm, and in the control group −1.9 mm (95% CI = −6 to 3 mm) Σ pop = 4 mm, σ pop = 2.1 mm. The average translational displacement of CCD in test group was 0 mm (95% CI = − 5.3 to 5.1 mm) Σ pop = 2.2 mm, σ pop = 2.6 mm. CCD translational displacement was greater in the unilateral arm abduction group at − 1.6 mm (95% CI = −6.7 to 3.4 mm) Σ pop = 3.6 mm, σ pop = 2.4mm.

Conclusion: The reduction in systematic error and inter-patient variability observed in the test group is evidence that bilateral arm abduction is a more stable and reproducible position than unilateral arm abduction. The CCD translational data indicates that patients treated with unilateral arm abduction were moving inferiorly on the breast board. These results support the adoption of bilateral arm abduction as a standard technique.

In the prehospital setting, the emergency care provider must anticipate that some patients will manifest with difficult airways. The use of video laryngoscopy to secure an airway in the prehospital setting has not been explored widely, but has the potential to be a useful tool. This article briefly reviews some of the major video laryngoscopes on the market and their usefulness in the prehospital setting. Studies and case reports indicate that the video laryngoscope is a promising device for emergency intubation, and it has been predicted that, in the future, video laryngoscopy will dominate the field of emergency airway management.

Direct laryngoscopy always should be retained as a primary skill; however, the video laryngoscope has the potential to be a good primary choice for the patient with potential cervical spine injuries or limited jaw or spine mobility, and in the difficult-to-access patient.The role of video laryngoscopes in securing an airway in head and neck trauma victims in the prehospital setting has yet to be determined, but offers interesting possibilities. Further clinical studies are necessary to evaluate its role in airway management by prehospital emergency medical services.

Purpose: To compare the Combifix® immobilization device with a conventional double-leg cushion in terms of patient comfort, therapist feedback and systematic/random error outcomes.

Materials and Methods: This prospective block-randomised crossover study enrolled 18 high-risk prostate cancer patients who received whole pelvic plus prostate radiotherapy. Treatment consisted of a prostate boost with one immobilization device followed by whole pelvic radiation using the other device. Our primary endpoints were device ease-of-use and patient comfort. Secondary endpoints included treatment time and systematic/random error assessments.

Results: While our patients found both devices equally comfortable and easy to use, the therapists preferred the leg cushion for ease of set-up (p = 0.04). Patient treatment time was similar for the two devices. In terms of electronic portal imaging (EPID)-based isocentre shifts, statistically, but not clinically, significant differences in systematic and random errors between the two devices exist in the superior–inferior directions (p ≤ 0.05).

Conclusions: No clinically important advantage was seen with the Combifix® device versus our standard double-leg cushion in terms of patient/therapist preference, patient comfort, and bony pelvic immobilization. However, this research project confirmed the feasibility of mounting a small single-institution randomised crossover technology assessment related to a practical radiotherapy issue.

Carbon and nutrient stocks in below-ground biomass have rarely been investigated in tropical montane forests. In the present study, the amounts of carbon, nitrogen, phosphorus, sulphur, potassium, calcium and magnesium in root biomass were determined by soil coring and nutrient analysis in forests at three altitudes (1900, 2400 and 3000 m) in the Ecuadorian Andes. Root biomass increased markedly from 2.8 kg m−2 at 1900 m and 4.0 kg m−2 at 2400 to 6.8 kg m−2 at 3000 m. The contribution of coarse roots (> 2 mm in diameter) to total root biomass increased from about 70% at 1900 m to about 80% at higher altitudes. In fine roots (≤ 2 mm in diameter), concentrations of nutrients except calcium markedly decreased with altitude. Therefore, the nutrient stocks in fine roots were similar at 1900 m and 3000 m for nitrogen and sulphur, and were even lower at higher altitudes for phosphorus, potassium and magnesium. In coarse roots of Graffenrieda emarginata concentrations of nutrients were substantially lower than in fine roots, and were little affected by altitude. The data suggest that the importance of coarse roots for long-term carbon and nutrient accumulation in total plant biomass increases with increasing altitude.