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Lactoperoxidase immobilization on silver nanoparticles enhances its antimicrobial activity

Published online by Cambridge University Press:  23 August 2018

Ishfaq Ahmad Sheikh ,
Muhammad Yasir ,
Imran Khan ,
Sher Bahadur Khan ,
Naveed Azum ,
Essam Hussain Jiffri ,
Mohammad Amjad Kamal ,
Ghulam Md Ashraf  and
Mohd Amin Beg
Ishfaq Ahmad Sheikh*
Affiliation:
King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia
Muhammad Yasir
Affiliation:
King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia
Imran Khan
Affiliation:
King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia
Sher Bahadur Khan
Affiliation:
Department of Chemistry, Faculty of Science, Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia
Naveed Azum
Affiliation:
Department of Chemistry, Faculty of Science, Center of Excellence for Advanced Materials Research, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia
Essam Hussain Jiffri
Affiliation:
Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia
Mohammad Amjad Kamal
Affiliation:
King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia
Ghulam Md Ashraf
Affiliation:
King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia
Mohd Amin Beg
Affiliation:
King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia
*
*For correspondence; e-mail: [email protected]
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Abstract

Lactoperoxidase (LPO) is an antimicrobial protein present in milk that plays an important role in natural defence mechanisms during neonatal and adult life. The antimicrobial activity of LPO has been commercially adapted for increasing the shelf life of dairy products. Immobilization of LPO on silver nanoparticles (AgNPs) is a promising way to enhance the antimicrobial activity of LPO. In the current study, LPO was immobilized on AgNPs to form LPO/AgNP conjugate. The immobilized LPO/AgNP conjugate was characterized by various biophysical techniques. The enhanced antibacterial activity of the conjugate was tested against E. coli in culture at 2 h intervals for 10 h. The results showed successful synthesis of spherical AgNPs. LPO was immobilized on AgNPs with agglomerate sizes averaging approximately 50 nm. The immobilized conjugate exhibited stronger antibacterial activity against E. coli in comparison to free LPO. This study may help in increasing the efficiency of lactoperoxidase system and will assist in identifying novel avenues to enhance the stability and antimicrobial function of LPO system in dairy and other industries.

Keywords

Antimicrobial activityimmobilizationlactoperoxidasesilver nanoparticles
Type
Research Article
Information
Journal of Dairy Research , Volume 85 , Issue 4 , November 2018 , pp. 460 - 464
Copyright
Copyright © Hannah Dairy Research Foundation 2018 

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