Five groups of four 8-week-old Blackface lambs were used to compare the effects of dietary deficiencies of calcium and/or phosphorus: four were given for 16 weeks a semi-purified diet low in Ca (0·07 %) and P (0·13 %), either unsupplemented (LCaLP) or supplemented with 0·3 % Ca (NCaLP), 0·3 % P (LCaNP) or both (NCaNP), and the remaining group was slaughtered initially.

The only clinical abnormality observed was twisting of the forelegs in group LCaLP. Food intake was depressed by 26 % in LCaNP group, by 41·5 % in NCaLP group but was largely unaffected in group LCaLP. Apparent D.M. digestibility was lower for group NCaLP (57·7 %) than for other groups (61·2 %). Differences in live weight and body fat reflected the differences in food intake between treatments but the weights of fat-free body components were lowest in the groups given low P diets.

The LCaNP diet decreased plasma Ca and increased plasma P but the NCaLP diet had the opposite effects. The LCaLP diet had no effect on plasma Ca and decreased plasma P less than the NCaLP diot.

The three mineral deficient diets caused equally marked decreases in Ca and P retention but did not induce negative balances; they also impaired the quality of bone in the tibia and lumbar vertebra, by reducing both the amount of bone matrix and the degree to which it was mineralized, and produced histological lesions of rickets and osteoporosis. Diets low in P caused a marked fall in Mg content of the skeleton.

Urolithiasis was common in lambs given diets adequate in P.

The Green Revolution era focused on enhancing the production and productivity of rice and wheat. New challenges demand that the issues of efficient resource use and resource conservation receive high priority to ensure that past gains can be sustained and further enhanced to meet the emerging needs. Extending some of the resource-conserving interventions developed for wheat to rice culture is a major challenge for researchers and farmers alike. The present paper shares recent research experiences on resource conservation technologies involving tillage and crop establishment options and associated agronomic practices which enable farmers in reducing production costs, increase profitability and help them move forward in the direction of adopting conservation agriculture.

The effects of water deficit on growth of spring barley were analysed under five irrigation treatments. One crop was irrigated at weekly intervals from emergence throughout the growing season, and one was not irrigated at all after emergence. Soil water deficits in the other treatments were allowed to develop early, intermediate or late in the crop's development.

Weekly irrigation produced a crop with a large leaf area index (maximum value 4) and maintained green leaf and awns throughout the grain-filling period. Early drought decreased leaf area index (maximum value 2) by slowing expansion of main-stem leaves and decreasing the number and growth of tiller leaves. Leaf senescence was also increased with drought. Drought late in the development of ears and leaves and during the grain-filling period caused leaves and awns to senesce so that the total photosynthetic areas decreased faster than with irrigation. Photosynthetic rate per unit leaf area was little affected by drought so total dry-matter production was most affected by differences in leaf area.

Early drought gave fewer tillers (550/m2) and fewer grains per ear (18) than did irrigation (760 tillers/m2 and 21 grains per ear). Late irrigation after drought increased the number of grains per ear slightly but not the number of ears/m2. Thus at the start of the grain-filling period crops which had suffered drought early had fewer grains than irrigated (9·5 and 18·8 × 103/m2 respectively) or crops which suffered drought later in development (14 × 103/m2).

During the first 2 weeks of filling, grains grew at almost the same rate in all treatments. Current assimilate supply was probably insufficient to provide this growth in crops which had suffered drought, and stem reserves were mobilized, as shown by the decrease in stem mass during the period. Grains filled for 8 days longer with irrigation and were heavier (36–38 mg) than without irrigation (29–30 mg). Drought throughout the grainfilling period after irrigation earlier in the season resulted in the smallest grains (29 mg).

Grain yield depended on the number of ears, the number of grains per ear and mass per grain. Early drought decreased tillering and tiller ear production and the number of grains that filled in each ear. Late drought affected grain size via the effects on photosynthetic surface area.

Drought decreased the concentrations of phosphorus, potassium and magnesium in the dry matter of crops, and irrigation after drought increased them. Concentration of nitrogen was little affected by treatment. Possible mechanisms by which water deficits and nutrient supply affect crop growth and yield are discussed.

Five species of rumen ciliate protozoa, isolated from sheep in which they were the only ciliate protozoon, and mixed rumen ciliates were incubated with labelled bacteria and the rate of uptake and digestion of the bacteria was followed. Evidence is presented that mixed rumen bacteria labelled by incubation with 14C-isoleucine, 14C-guanine or 35S-sulphate were engulfed more rapidly by the protozoa than those labelled with 14C-glucose but that, with Entodinium spp. at least, this apparent lower rate of uptake was associated with an increased rate of digestion. The rate of uptake of mixed rumen bacteria (at 109/ml) varied from 230 to 2670 bacteria/protozoon per hour, depending on the protozoal species, and increased with increasing bacterial population density. Starvation of the protozoa decreased the initial rate of uptake of bacteria by up to 60% and the length of time over which the protozoa would engulf bacteria. Entodinium spp. and Epidinium ecaudatum caudatum engulfed fewer bacteria when these bacteria were prepared from the same sheep as the protozoa than when they were prepared from sheep with no or other protozoal populations. Of the protozoa tested, only Entodinium spp. and the mixed large rumen protozoa digested mixed rumen bacteria, Eremoplastron bovisand Epidinium ecaudatum caudatum being inactive. It is suggested that previous estimates of the rate of uptake and digestion of bacteria based on studies in vitro were too high.

The non-rumen bacteria Eschericfoia coli and Klebsiella aerogenes and the rumen bacterium Megasphaera elsdenii were not or only poorly taken up by the protozoa. Entodinium spp. selectively engulfed bacteria of rumen origin and digested two cellulolytic bacteria rapidly. In contrast, Eremplastron bovis engulfed these cellulolytio bacteria more slowly than other bacteria. Entodinium spp. and the large rumen protozoa digested more bacterial species than the other protozoa but Butyrivibrio fibrisolvens was digested more rapidly than other bacteria by all the protozoa.

Blood samples were taken from groups of lactating and non-lactating cows on three occasions from each of 24 commercial dairy herds sampled eight herds at a time at 6-weekly intervals throughout 1972. The blood samples were analysed for packed cell volume, blood glucose and haemoglobin, and for the serum concentrations of albumin, total protein, urea nitrogen, Ca, inorganic phosphate, Mg, K, Na, Cu, and Fe.

Clear seasonal patterns were evident for packed cell volume and the mean concentrations of urea nitrogen and haemoglobin each of which were highest during the summer months. The effect of season on the concentrations of the other constituents was smaller. Mean values of packed cell volume, haemoglobin and Fe were consistently higher in the non-lactating cows than in the lactating cows. Mean concentrations of Mg were lower in the blood of non-lactating cows throughout the year; Cu concentrations followed a similar trend in the autumn and winter.

There was evidence of overall herd differences regardless of season, in particular for Cu, globulin, Fe and urea nitrogen concentrations.

Two-hundred-and-seventy-three Scottish Blackface ewes in three ages (5, 4 and 2 years) were differentially group fed over 6 weeks in such a manner that at 6 weeks before mating there were approximately equal numbers in each of six subjectively assessed body condition groups ranging from score 1 (lean) to score 3½ (moderately fat). Over the 6 weeks prior to mating these groups were either well fed, fed at about maintenance, or underfed so that at mating the maj ority of ewes were in either condition 1½ or 3.

A predetermined random sample of mature ewes from each of the six original condition groups was killed 2–3 days post-mating for ovulation counts. Surviving ewes were fed above maintenance post-mating and the number of lambs recorded at birth. Condition at mating had a significant, positive effect on both ovulation and lambing rates but had no effect on infertility measured as the difference between them.

The level of food intake prior to and at mating had no effect on either ovulation or lambing rates of moderately fat ewes (condition 3) but the results can be interpreted as indicating that the level of food intake had a positive effect on both ovulation and lambing rates of lean ewes (condition 1½).

A three factor experiment with winter wheat (Triticum aestivum L.) was carried out during two years on a coarse sandy soil in Denmark. The factors comprised four irrigation strategies including no irrigation, three nitrogen levels providing 67, 83 or 100% of the recommended nitrogen rate, and two strategies for control of leaf diseases (with and without fungicides). Different varieties were used in the two years, Pepital in the first year and Hussar in the second year. Mildew dominated the trial in the first year, but was almost absent in the second year. Septoria occurred in both years, but most severely in the second year. Irrigation increased grain yield, but there were no significant differences between the three strategies, where irrigation was applied. The effect of irrigation on yield was almost solely via an effect of increased transpiration, whereas water use efficiency and harvest index was unaffected. There was a significant interaction for grain yield between irrigation and nitrogen strategies with higher irrigation effects at higher nitrogen rates. This interaction was absent at high disease levels. Increasing nitrogen rate increased grain yield in the second year, but not in the first year. This was attributed to an increasing mildew incidence with increasing nitrogen rate. Irrigation also increased mildew incidence. This caused significant interactions for grain yield between fungicide application and nitrogen rate and between fungicide application and irrigation strategy. Septoria was also significantly affected by both nitrogen and irrigation strategies, but to a lesser degree and not in a consistent manner. The main effect of disease on grain yield was through a reduction in harvest index and a reduction in grain weight. Increasing nitrogen rates slightly reduced harvest index both on a dry matter and on a nitrogen basis. The interaction effects were smaller than the effects of the main factors, and the effect of changes in irrigation or nitrogen strategy on disease incidence could not substitute for the effective disease control obtained by fungicides. The results do, however, indicate possibilities of improving the efficiency of current decision support systems for both fungicide control by better timing of application and irrigation scheduling by reducing water application.

Mature Merino ewes (n = 320) were fed either maintenance (100% M) or 25% maintenance (25% M) rations from the day after mating until embryos were collected at either day 11 or 21 after mating. On the 4th day after mating 223 ewes were ovariectomized and given 5, 10, 15, 20 or 25 mg progesterone/day (intramuscular) until embryo collection. A further group of sham operated ewes (n = 54) acted as controls. Despite live-weight losses of 10·7 and 15·8% from mating to days 11 and 21 after mating respectively, ewes on 25% M rations showed no evidence that embryonic survival was influenced by undernutrition. The embryos from ewes fed 25 % M rations were significantly (P < 0·05) smaller than those fed 100% M (crown-rump measurements were 5·93 ± 0·139 mm and 6·39 ± 0·116 mm at day 21 after mating in the 25 % M and 100% M groups respectively). Development, assessed by formation of the first pharangeal lobe and the fore- and hind-limb buds, was significantly retarded (P < 0·05) in the 21-day-old embryos from the undernourished ewes. Embryo survival in ovariectomized ewes receiving 5 or 10 mg progesterone/day was significantly (P < 0·05) lower than in ovariectomized ewes receiving 20 and 25 mg progesterone/ day at day 11 after mating. Embryo survival at both days 11 and 21 after mating increased with increasing doses of progesterone. Ewes fed 25% M rations had elevated (P< 0·001) concentrations of peripheral progesterone compared with those fed 100% M. This occurred when the progesterone was derived from either exogenous or endogenous (ovarian) sources, dismissing the possibility that changes in secretion rates could account for these observed differences. Differences in embryo growth and development were not related to these variations in the plasma progesterone concentration. Undernutrition restricted both growth and development to 21 days of pregnancy.

In spite of the entomophilic structure of the rape flower, which is very attractive to nectar- and pollen-gathering bees, the crop gives good yields without insect pollination.

The aim of this study was to determine whether the inclusion of a condensed tannin (quebracho tannin, QT) and/or the elevation of dietary protein could reduce Trichostrongylus colubriformis establishment and existence in the small intestine of lambs. Thirty-six lambs (mean liveweight 32·6±3·9 kg) were randomly allocated to one of six experimental groups, groups 1–5 were parasitized with a trickle infection of 3000 infective Trichostrongylus colubriformis larvae daily, whilst group 6 remained as uninfected controls. Experimental diets were formulated to contain 222 g CP/kg (high protein) or 97 g CP/kg (low protein) with or without the inclusion of 50 g QT/kg. All six animal groups were fed the low protein diet, group 2 fed low protein diet+QT, for one month prior to infection (groups 1–5). Once nematode eggs were observed in the faeces, diets were abruptly changed in three experimental groups. Group 1 remained on the low protein diet, group 2 remained on the low protein+QT diet, group 3 changed to the high protein diet, group 4 changed to the high protein+QT diet, group 5 changed to the low protein+QT diet and group 6 remained uninfected and fed the low protein diet. Production, haematological and parasitological parameters were monitored at regular intervals. Results show that parasitized animals fed the high protein diet achieved growth rates similar to those of uninfected low protein-fed lambs. Inclusion of dietary QT did not depress liveweight gain. Total daily faecal egg counts declined after feeding the high protein diet. Inclusion of QT into the low protein diet also reduced faecal egg counts to similar levels observed in the high protein-fed lambs. The inclusion of QT into the high protein diet did not further reduce faecal egg counts. No significant differences in the haematological parameters measured were observed between infected animals (groups 1–5), suggesting that the beneficial effect of dietary QT in the low protein diet is unlikely to be mediated through an immune response. These data suggest that the inclusion of QT in low protein diets may be an alternative to feeding high protein diets to reduce nematode burden in lambs.

The effect of daily herbage allowance on herbage intakes and growth rates lambs grazing perennial ryegrass and red clover was investigated in two experiments. Herbage allowances defined as g herbage D.M./kg live weight (LW)/day were controlled by varying the areas of plots grazed for 2 days by groups of six lambs.

In the first experiment five herbage allowances in the range 20–120 g D.M./kg LW/day were offered on two areas of a perennial ryegrass (cv. S. 23) sward that received nitrogen fertilizer applications of 39 or 78 kg N/ha/28 days. In the second experiment five herbage allowances in the range 30–160 g D.M./kg LW/day were offered on perennial ryegrass (cv. S. 23) and red clover (cv. Hungaropoly) swards.

Asymptotic curves were fitted to describe the relationship between herbage allowance and daily intake of herbage. In Expt 1 nitrogen fertilizer rates did not affect the yield of herbage or animal performance. In Expt 2 intakes were higher on the clover sward than on the ryegrass sward at the higher herbage allowances.

The asymptotic curves to describe the relationship between herbage allowance and growth rate of lambs differed widely between periods. Growth rate of the lambs increased linearly with increase in digestible organic matter intake. Live-weight gain per unit of digestible organic matter intake was higher on the red clover than on the ryegrass.

The conclusion is drawn that if the herbage present to ground level is not more than three times the daily intake of the animals, intake of herbage of the animals may bo restricted.

The rust diseases have plagued cereal cultivation from the early days of domestication. Biblical accounts report on the plight of Hebrews resulting from severe rust epidemics, while the ancient Greeks and Romans made offerings to the corn god, Robigus in attempts to defeat these devastating diseases (McIntosh et al. 1995). Man's approaches to fighting plant disease have changed since the time of the Romans, but still the fight continues. While we may have won many battles, the war is not yet over.

Multi-environment trials (METs) in West Africa have demonstrated the existence of genotype×environment interactions (G×E), which complicate the selection of superior cultivars and the best testing sites for identifying superior and stable genotypes. Two powerful statistical tools available for MET analysis are the additive main effects and multiplicative interaction (AMMI) and the genotype main effect+G×E (known as GGE) biplot. The objective of the present study was to compare their effectiveness in identifying maize mega-environments and stable and superior maize cultivars with good adaptation to West Africa. Twelve extra-early maturing maize cultivars were evaluated at 17 locations in four countries in West Africa from 2006 to 2009. The effects of genotype (G), environments (E) and G×E were significant (P<0 01) for grain yield. Differences between E accounted for 0 75 of the total variation in the sum of squares for grain yield, whereas the G effects accounted for 0 03 and G×E for 0 22. The GGE biplot explained 0 74 of total variations in the sum of squares for grain yield and revealed three mega-environments and seven cultivar groups. The AMMI graph explained 0 13 and revealed four groups each of environments and cultivars. The two procedures provided similar results in terms of stability and performance of the cultivars. Both methods identified the cultivars 2004 TZEE-W Pop STR C4 and TZEE-W Pop STR C4 as superior across environments. Cultivar 2004 TZEE-W Pop STR C4 was the most stable. The GGE biplot was more versatile and flexible, and provided a better understanding of G×E than the AMMI graph. It identified Zaria, Ilorin, Ikenne, Ejura, Kita, Babile, Ina and Angaredebou as the core testing sites of the three mega-environments for testing the Regional Uniform Variety Trials-extra-early.

Several delignifying agents, used in various paper-making processes, have been compared for their ability to remove lignin and increase the digestibility of several roughages in laboratory experiments using a spray method of treatment recently suggested by Wilson & Pigden (1964). The chemicals were dissolved in small quantities of water which were then used to moisten the roughage. No washing was done after treatment. Sodium hydroxide was the most effective chemical; at a rate of 10 g per 100 g of roughage, the lignin content of ground maize cobs was reduced by 26% and rumen dry-matter digestibility increased by more than 100%. Bleaching powder was as effective as sodium hydroxide in removing lignin, but residual chlorine apparently inhibited rumen microbial digestion.

Maize cobs, wheat and paddy straws, sorghum stover and sugar cane tops, though of widely differing initial digestibility, were brought to an equal level of rumen digestibility if treated with 4% or more of sodium hydroxide. Drying the treated roughage at 100 degrees instead of at room temperature increased digestibility only in the case of maize cobs. The alkali spray treatment was as effective in increasing the digestibility of chaffed as of ground wheat straw. Data are presented which show the amounts of residual alkali in treated roughage as a function of time after treatment and of concentration of alkali used in the treatment. Further, data are presented which show the loss of nutrients from treated roughage if washed as in the traditional method.

In experiments at Sutton Bonington between 1967 and 1970, which tested 0–300 kg N/ha applied in spring, maximum oil yields of both autumn- and spring-sown oilseed rape were obtained by applying 200 kg N/ha. The seed-oil content was reduced by nitrogen application but only seriously when 300 kg/a was applied. Responses to high levels of potassium (180 rather than 60 kg K20/ha) were only obtained at 200 kg N/a. Successive increments of nitrogen from nil to 300 kg/ha improved plant growth, increasing leaf area and the dry weight of leaves and stems. The last increment from 200 to 300 kg N/ha was less effective and either reduced or did not affect pod production.

In one experiment 300 kg N/ha stimulated husk growth, apparently at the expense of seed growth, with 200 kg/ha giving the highest proportion of pod dry matter as seed. More nitrogen always raised number of seeds per pod, but only increased seed weight in one autumn-sown experiment.

Serial harvests of one experiment indicated that the maximum seed and oil yield was obtained by cutting plants before they were fully ripe, thus avoiding seed loss through pod shatter. The oil content of the bulk samples reached a peak about a week before maximum oil yield, probably because later-ripening seed did not reach full maturity. All nitrogen levels delayed flowering, but only the highest delayed maturity in the autumn-sown crop.

Mixed intercropping of spring barley (Hordeum vulgare L.) with field pea (Pisum sativum L.), faba bean (Vicia faba var. minor L.) or narrow-leafed lupin (Lupinus angustifolius L.) was compared with sole cropping in two field experiments at different locations, on a sandy loam soil and a sandy soil, in Denmark in 2001.

Grain legumes were dominant in intercrops on the sandy loam soil, except for lupin, whereas barley was dominant in intercrops on the sandy soil site. Combined intercrop grain yields were comparable to grain yields of the respective sole cropped grain legume or sole cropped, fertilized barley on each soil site. On the sandy loam soil, pea–barley and faba bean–barley intercrops increased the proportion of plant N derived from N2 fixation in grain legumes and increased the barley grain N concentration (from 1·7 to 2·2 mg/g) compared with sole cropping. However, the later maturity of faba bean compared with barley caused problems at harvest. The grain N concentration of intercropped barley was increased where grain legumes were the dominant intercrops and not on the sandy soil site. Lupin-barley intercrops did not show intercropping advantages to the same degree as faba bean and pea, but lupin constituted a more stable yield proportion of the combined intercrop yield over locations.

Furthermore, the study indicated that the natural 15N abundance at certain locations might not always be sufficient to ensure a reliable estimate of N2 fixation using the 15N natural abundance method.

Seeds of oilseed rape (Brassica napus L.) can persist in the soil over several years by becoming secondarily dormant and can then germinate to create volunteer plants in following crops. As well as agricultural impacts caused by volunteers, gene dispersal in time – particularly from genetically modified cultivars – can be another undesirable consequence. Conventionally bred and transgenic seeds were tested in 2001 and 2002 in laboratory experiments, and in a field experiment, by burying seeds in the soil to determine the variation in dormancy and persistence capacity.

In the conventional group of cultivars tested in the laboratory, the level of dormancy was 13–76% in 2001, and 3–76% with an extended group in 2002. The transgenic group of cultivars was 1–31% dormant. In the burial experiments the number of viable seeds recovered in the conventionally bred cultivars ranged from 34–90% in 2001, and 7–68% in 2002. In the same studies the transgenic cultivars developed persistence levels from 12–79% in 2001, and 46–67% in 2002. Since dormancy levels of conventionally bred cultivars from 2 harvest years in the laboratory tests correlated significantly (r=0·71), it appears that there is a genetic background to secondary dormancy. There was also a significant correlation (r=0·61 in 2001 and 0·80 in 2002) between the results from laboratory and burial experiments. This indicates that the laboratory approach can simulate the situation in the field. Ageing over 6 months decreased the capacity for seed persistence to about a fifth of the level shown when freshly harvested. As a consequence of ageing and environmental impacts on persistence, only seeds from the same location and harvest year should be used for testing genetic variability. The high genetic variability among currently available rape seed cultivars gives breeding strategies a good chance of ideotyping low persistence genotypes and minimizing the risk of gene dispersal in time.

Four field experiments were conducted to investigate the effects of seed rate on yield and quality of wheat. Despite some small and inconsistent effects of seed rate on radiation-use efficiency and harvest index, the responses of PAR interception, above-ground biomass and grain yield generally followed similar asymptotic increases as seed rate increased. In one experiment, when nitrogen fertilizer was withheld, biomass and grain yields did not respond to increases in seed rate despite increases in PAR interception. In one experiment, grain yield followed a parabolic response to seed rate with apparent reductions in yield at very high seed rates. Plants compensated for low population densities by increased production and survival of tillers and, to a lesser extent, increased grain numbers per ear. Net tiller production continued until the main stems flowered or later. Effects of seed rate on grain specific weight and thousand grain weight were small and inconsistent. Hagberg falling number increased linearly with seed rate in three experiments, associated with quicker maturation of the crop. Grain protein concentration declined with increase in sowing rate according to linear divided by linear or linear plus exponential models depending on whether the grain yield response was asymptotic or parabolic. Discolouration of the grain with blackpoint increased with seed rate in the most susceptible cultivar, namely Hereward. The economic consequences of these effects on yield and quality are discussed.

Nineteen feedstuffs were evaluated for total digestible nutrients (TDN), digestible energy (DE) and metabolizable energy (ME) using growing pigs. DE values, on a drymatter basis, ranged from 1570 kcal/kg D.M. (grassmeal) to 4890 kcal/kg D.M. (BP Grangemouth protein). ME averaged 97·4% of DE for cereal feeds and 81·9% of DE for protein feeds, the ratio of DE to ME being significantly affected by the crudeprotein content of the feedstuff:

ME/DE × 100 = 99·7–0·18 × crude protein (%): r = –0·94; P < 0·01.

TDN was more closely related to ME than DE:

DE (kcal/kg D.M.) = 45 TDN + 156: r = 0·92; P < 0·01,

ME (kcal/kg D.M.) = 43 TDN + 71: r = 0·98; P < 0·01.

ME values were corrected to both 30% and 0% nitrogen retention. Correction to 30% retention caused a maximum change of 3% in ME value while correction to 0% retention had a variable effect related to protein content of the feedstuff:

(ME(N0)/ME) × 100 = 98·3–0·113 × crude protein (%): r = –0·80; P < 0·01.