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The engulfment and digestion of mixed rumen bacteria and individual bacterial species by single and mixed species of rumen ciliate protozoa grown in vivo

Published online by Cambridge University Press:  27 March 2009

G. S. Coleman  and
Deborah C. Sandford
G. S. Coleman
Affiliation:
Biochemistry Department, A.R.C. Institute of Animal Physiology, Babraham, Cambridge CB2 4AT
Deborah C. Sandford
Affiliation:
Biochemistry Department, A.R.C. Institute of Animal Physiology, Babraham, Cambridge CB2 4AT
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Summary

Five species of rumen ciliate protozoa, isolated from sheep in which they were the only ciliate protozoon, and mixed rumen ciliates were incubated with labelled bacteria and the rate of uptake and digestion of the bacteria was followed. Evidence is presented that mixed rumen bacteria labelled by incubation with 14C-isoleucine, 14C-guanine or 35S-sulphate were engulfed more rapidly by the protozoa than those labelled with 14C-glucose but that, with Entodinium spp. at least, this apparent lower rate of uptake was associated with an increased rate of digestion. The rate of uptake of mixed rumen bacteria (at 109/ml) varied from 230 to 2670 bacteria/protozoon per hour, depending on the protozoal species, and increased with increasing bacterial population density. Starvation of the protozoa decreased the initial rate of uptake of bacteria by up to 60% and the length of time over which the protozoa would engulf bacteria. Entodinium spp. and Epidinium ecaudatum caudatum engulfed fewer bacteria when these bacteria were prepared from the same sheep as the protozoa than when they were prepared from sheep with no or other protozoal populations. Of the protozoa tested, only Entodinium spp. and the mixed large rumen protozoa digested mixed rumen bacteria, Eremoplastron bovisand Epidinium ecaudatum caudatum being inactive. It is suggested that previous estimates of the rate of uptake and digestion of bacteria based on studies in vitro were too high.

The non-rumen bacteria Eschericfoia coli and Klebsiella aerogenes and the rumen bacterium Megasphaera elsdenii were not or only poorly taken up by the protozoa. Entodinium spp. selectively engulfed bacteria of rumen origin and digested two cellulolytic bacteria rapidly. In contrast, Eremplastron bovis engulfed these cellulolytio bacteria more slowly than other bacteria. Entodinium spp. and the large rumen protozoa digested more bacterial species than the other protozoa but Butyrivibrio fibrisolvens was digested more rapidly than other bacteria by all the protozoa.

Type
Research Article
Information
The Journal of Agricultural Science , Volume 92 , Issue 3 , June 1979 , pp. 729 - 742
Copyright
Copyright © Cambridge University Press 1979

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References

REFERENCES

Abou Akkada, A. R., Bartley, E. E., Berube, R., Fina, L. B., Meyer, R. M., Hendricks, D. & Julius, F. (1968). Simple method to remove completely ciliate protozoa of adult ruminants. Applied Microbiology 16, 1475–1477.CrossRefGoogle Scholar
Coleman, G. S. (1958). Maintenance of oligotrich protozoa from the sheep rumen in vitro. Nature, London 182, 1104–1105.CrossRefGoogle ScholarPubMed
Coleman, G. S. (1964). The metabolism of Escherichia coli and other bacteria by Entodinium caudatum. Journal of General Microbiology 37, 209–223.Google Scholar
Coleman, G. S. (1967a).The metabolism of free amino acids by washed suspensions of the rumen ciliate Entodinium caudatum. Journal of General Microbiology 47, 433–447.Google Scholar
Coleman, G. S. (1967b). The metabolism of the amino acids of Escherichia coli and other bacteria by the rumen ciliate Entodinium caudatum. Journal of General Microbiology 47, 449–464.Google Scholar
Coleman, G. S. (1972). The metabolism of starch, glucose, amino acids, purines, pyrimidines and bacteria by the rumen ciliate Entodinium simplex. Journal of General Microbiology 71, 117–131.Google Scholar
Coleman, G. S. (1975). The interrelationships between rumen ciliate protozoa and bacteria. In Digestion and Metabolism in the Ruminant (ed. McDonald, I. W. and Warner, A. C. I.), pp. 150–164. Sydney: The University of New England Publishing Unit.Google Scholar
Coleman, G. S. (1978). The cultivation of rumen Entodiniomorphid protozoa. In Methods of Cultivating Parasites in vitro (ed. Baker, J. R. and Taylor, A. E. R.), pp. 39–54. London: Academic Press.Google Scholar
Coleman, G. S. & Laurie, J. I. (1974). The metabolism of starch, glucose, amino acids, purines, pyrimidines and bacteria by three Epidinium spp. isolated from the rumen. Journal of General Microbiology 85, 244–256.CrossRefGoogle Scholar
Coleman, G. S. & Laurie, J. I. (1977). The metabolism of starch, glucose, amino acids, purines, pyrimidines and bacteria by the rumen ciliate Polyplastron multivesiculatum. Journal of General Microbiology 98, 29–37.CrossRefGoogle Scholar
Eadie, J. M. (1962). Inter-relationships between certain ciliate protozoa. Journal of General Microbiology 29, 579–588.CrossRefGoogle Scholar
Eadie, J. M. (1967). Studies on the ecology of certain rumen ciliate protozoa. Journal of General Microbiology 49, 175–194.CrossRefGoogle ScholarPubMed
Eadie, J. M., Hyldegaard-Jensen, J., Mann, S. O., Reid, R. S. & Whitelaw, F. G. (1970). Observations on the microbiology and biochemistry of the rumen of cattle given different quantities of a pelleted barley ration. British Journal of Nutrition 24, 157–176.Google ScholarPubMed
Hobson, P. N. (1969). Rumen bacteria. Methods in Microbiology 3B, 133–149.CrossRefGoogle Scholar
Kurihara, Y., Takechi, T. & Shibata, F. (1978). Relationship between bacteria and ciliate protozoa in the rumen of a sheep fed a purified diet. Journal of Agricultural Science, Cambridge 90, 373–382.CrossRefGoogle Scholar
Warner, A. C. I. (1962). Enumeration of rumen microorganisms. Journal of General Microbiology 28, 119–128.CrossRefGoogle Scholar
White, R. W. (1969). Viable bacteria inside the rumen ciliate Entodinium caudatum. Journal of General Microbiology 56, 403–408.CrossRefGoogle ScholarPubMed
White, R. W. (1977). Microbial ethanol, yeasts and coliform bacteria in the stomachs of milk fed calves. Journal of Applied Bacteriology 42, 423–427.CrossRefGoogle ScholarPubMed