Exotic plants have the ability to modify soil seed banks in habitats they invade, but little is known about the legacy of invasion on seed banks once an exotic plant has successfully been controlled. Natural areas previously invaded by leafy spurge in the northern Great Plains typically have one of two fates following its removal: a return of native plants, or a secondary invasion of other exotic plants. It is unknown, however, if this difference in plant communities following leafy spurge control is due to seed bank differences. To answer this question, we monitored seed banks and standing vegetation for 2 yr in mixed-grass prairies that were previously invaded by leafy spurge but controlled within 5 yr of our study. We found that native plant seed banks were largely intact in areas previously invaded by leafy spurge, regardless of the current living plant community, and leafy spurge invasion history had a larger impact on cover and diversity of the vegetation than on the seed banks. Differences in plant communities following leafy spurge control do not appear to be related to the seed banks, and soil conditions may be more important in determining trajectories of these postinvasion communities.

Developing management strategies for invasive plant species requires identifying effective treatment methods (e.g., physical or chemical treatments) and optimally timing their application. For invasive annual plants, effects of treatments on seed production and germinability are paramount to reduce seed banks and subsequent plant establishment. We compared effects of a range of physical and chemical treatments applied to plants containing seeds at different developmental stages at field sites in the Mojave Desert (Nevada and Arizona) for managing the exotic annual forb, African mustard. Effectiveness of physical treatments (hand pulling entire plants, breaking plants, or separating siliques from plants) for reducing African mustard seed size and germination varied by treatment and seed maturity stage at which treatments were applied. With all treated plant material remaining in the field (i.e., not bagged and transported off site), hand pulling or breaking plants resulted in 90 to 100% of developing and developed seeds still able to germinate. Separating siliques from plants, however, resulted in 0% germination of undeveloped and developing seeds. All three tested herbicides (glyphosate, 2,4-D, and metsulfuron) reduced germination to zero or near zero across all seed development stages. Results suggest that physical treatments are most effective at early stages of seed development, whereas several different herbicides are effective across seed developmental stages. Results also highlight the importance of considering the potential for continued seed development and germinability on treated plant material when choosing invasive plant treatment types and timing.

Downy brome (cheatgrass) is a highly successful, exotic, winter annual invader in semi-arid western North America, forming near-monocultures across many landscapes. A frequent but poorly understood phenomenon in these heavily invaded areas is periodic ‘die-off’ or complete stand failure. The fungal pathogen Pyrenophora semeniperda is abundant in cheatgrass seed banks and causes high mortality. To determine whether this pathogen could be responsible for stand failure, we quantified late spring seed banks in die-off areas and adjacent cheatgrass stands at nine sites. Seed bank analysis showed that this pathogen was not a die-off causal agent at those sites. We determined that seed bank sampling and litter data could be used to estimate time since die-off. Seed bank patterns in our recent die-offs indicated that the die-off causal agent does not significantly impact seeds in the persistent seed bank.

Old World climbing fern (OWCF) is a highly invasive plant that is spreading rapidly and disrupting natural areas in Florida, with the potential to spread by wind-blown spores into Central and South America. Experiments were performed using gametophytes to determine the dose response of OWCF to metsulfuron methyl (metsulfuron). OWCF gametophytes were highly susceptible to metsulfuron with ≤ 1.4% survival at concentrations ≥ 27 mg ai L−1. Metsulfuron OWCF gametophyte 50 and 95% inhibition (I50 and I95) values were 6.1 and 26.4 mg ai L−1, respectively. Survival of treated gametophytes that developed into sporophytes was ≤ 0.014% at concentrations ≥ 27 mg ai L−1, but no sporophytes developed at concentrations ≥ 432 mg ai L−1. Metsulfuron OWCF sporophyte inhibition I50 and I95 values were 5.6 and 24.1 mg ai L−1, respectively. At 216 mg ai L−1 metsulfuron, half of the standard operational use rate, 1.1 × 10−8% of the treated gametophytes developed into sporophytes. The results indicate that the potential for tolerant, and potentially resistant, OWCF populations is higher using lower concentrations of metsulfuron as no gametophytes developed into sporophytes at the standard field treatment concentration of 432 mg ai L−1.

Predicting site vulnerability to nonnative plant establishment remains a difficult goal. Seedling survival is an important component of population dynamics and can affect the success of control strategies. Field manipulations allow potential causal mechanisms of site vulnerability to be evaluated under realistic environmental conditions. We conducted field studies to determine the effects of plant competition and differing precipitation regimes on spotted knapweed seedling survival. We also examined the effect of herbivory on rosette survival and growth. Seeds were sown into plots with vegetation intact or removed at three sites. Seeds were also sown into plots where plant competition and precipitation were manipulated in a factorial design at a single site. Field studies demonstrated that site accounted for much of the variation in emergence rate, while herbivory and plant competition affected seedling survival rates. We observed a wide range in emergence rates, with site averages ranging from 13.1 to 42.5%. Survival the following year ranged from 0.5 to 9.4% of sown seeds. Rosette survival was significantly higher when herbivores were excluded from plots. Below average precipitation reduced seedling survival; however, even with supplemental water, dry-down of exposed sites resulted in low seedling survival. Of the 8,000 seeds added to plots in one study, by autumn, only eight plants resulted, seven of which survived in watered plots with intact vegetation. Collectively, these results show that seedling survival is a critical phase in spotted knapweed population dynamics and can vary among habitats on the basis of plant competition and precipitation. Furthermore, herbivory affects all stages of the lifecycle from the seedling onward. The observed differences help explain the reported variability in seedling survival in the literature and inform efforts to control spotted knapweed using plant competition and biological controls.

Privet has escaped from cultivation and is invading natural areas throughout eastern North America. Understanding the pattern of invasion over time could help us develop more efficient management strategies. We studied the invasion history and spatial distribution pattern of privet by mapping age and spatial data for established patches in a 132-ha (326 ac) forested natural area in northeast Ohio. We determined the age of 331 geo-referenced patches by counting annual rings, and mapped them with corresponding land habitat. Age distribution and cumulative number of privet patches over about 40 yr showed three phases of invasion. The initial 19-yr lag phase was characterized as a dispersed spatial pattern (based on nearest neighbor analysis), with patches located mostly at edges of different habitats and open places. In a second phase of about 15 yr, an average of 19 patches were initiated yearly, in a pattern that trended towards clustered. The final phase began around 2007, as the rate of new patch establishment declined, possibly because of saturation of the suitable habitat. Establishment of new patches was not associated with specific habitats. Aggregation of patches with similar ages increased after 1998 and became significantly clustered. Mapping of clusters of old and young patches identified invasion hot spots and barriers. Results affirmed that the best time for invasive control is during the lag phase. By monitoring edge habitats associated with early establishment, managers might detect and control early invaders and delay the onset of the expansion phase.

Seed dispersal is a crucial process in most plant invasions, but is notoriously difficult to study. One technique to identify the maternal source of dispersed seeds and newly established seedlings is labeling with a stable isotope. We tested whether foliar application of 15N-labeled urea would result in sufficient 15N enrichment to discriminate among seeds and seedlings grown from those seeds of the invasive shrub Lonicera maackii (Amur honeysuckle). We subjected mature L. maackii to all combinations of three concentrations of 15N-labeled urea (0.025 g L−1 [0.003 oz gal−1], 0.20 g L−1, and a 0 g L−1 control) and three temporal treatments (one application in August, one application in September, and five applications spaced every three weeks from June through August). Seeds were collected September to November; some of these were analyzed for %15N and others allowed to germinate and grow into seedlings under two treatments (in potting mix in greenhouse and in woodlot soil outdoors). Seedlings were harvested midway through the next growing season. We found that seeds from plants subjected to the three different concentrations had significantly different %15N levels, and there was a significant interaction between concentration and temporal treatment: the highest seed %15N levels were from plants sprayed five times with 15N-labeled urea, and the second highest from plants sprayed once in September. Similar patterns in %15N levels were found in seedlings, except that those from the 0.025 g L−1 spray treatment were only distinguishable from controls for seedlings grown outdoors in woodlot soil. These findings demonstrate that a single foliar application of 15N in early September is sufficient to label both seeds and seedlings of this invasive shrub, enabling one to identify the source of field-collected seeds or seedlings. This provides a tool for studying patterns and processes in seed dispersal of Amur honeysuckle and potentially other invasive plants.

We assessed pre- and posteradication nitrogen and phosphorus dynamics in longleaf pine sandhill stands severely affected by cogongrass. Across a 7-yr posteradication (glyphosate + imazapyr) “recovery chronosequence,” which included untreated cogongrass, uninvaded reference, and treated plots, we analyzed soils for total N, potentially available P (Mehlich-1 [M1]), pH, and organic matter content. We also used resin bags to assess fluxes of plant available N and P in the soil solution. Additionally, we used litterbags to monitor the decomposition and nutrient mineralization patterns of dead rhizome and foliage tissue. Our results indicate similar total N and M1-P contents in both cogongrass-invaded and uninvaded reference plots, with levels of M1-P being lower than in cogongrass plots for 5 yr after eradication. Soil organic matter did not differ between treatments. Resin bag analyses suggest that cogongrass invasion did not affect soil nitrate availability, although a pulse of NO2 + NO3 occurred in the first 3 yr after eradication. No such trends were observed for ammonium. Resin-adsorbed PO4 was lowest 3 yr after eradication, and pH was highest 5 yr after eradication. Our litterbag study showed that approximately 55% of foliar biomass and 23% of rhizome tissue biomass remained 18 mo after herbicide treatment. Substantial N immobilization was observed in rhizomes for the first 12 mo, with slow mineralization occurring thereafter. Rapid P mineralization occurred, with 15.4 and 20.5% of initial P remaining after 18 mo in rhizomes and foliage, respectively. Overall, our findings indicate that cogongrass invasion has little to no effect on soil nutrient cycling processes, although some significant—but ephemeral—alterations develop after eradication.

To investigate the impacts of long-term targeted invasive plant stewardship projects on students' subsequent stewardship attitudes, an experiment using pre and post-tests to understand program effects and using post-test only comparisons to understand school effects was conducted. The resulting scores from two science classes that participated in year-long invasive plant and restoration activities were compared with those from three comparable classes at a linked school that did not participate in any of these activities. Students in the experimental classes showed overall significantly higher scores compared with the control classes. These attitude scores were then divided into two indexes: sense of personal effectiveness and attitudes of caring for particular places. Students in the experimental group showed increases in both, as compared with the controls. Parent and student focus groups were conducted at the end of the academic year. The resulting comments provide evidence for actual behavior change outside of the school environment. Analysis showed that any student, especially those in the control classes in the traditional middle school, indicating they had prior exposure to nature stewardship projects showed significantly higher scores than students who did not. The results underscore the value of having students involved in real-world stewardship projects, especially those of a long-term nature.

The presence of landscape disturbances increases the establishment of exotic plants inside natural areas. Here, we examine the effect of human disturbances to prairie landscapes on the distribution and abundance of Kentucky bluegrass and smooth brome, exotic grasses that threaten the integrity of prairie ecosystems throughout the northern Great Plains. Using plant inventory data from Riding Mountain National Park in Manitoba, Canada, we investigated how roads, trails, and the intensity of historic livestock grazing affect the distribution and abundance of exotic grasses. On the basis of our results, both Kentucky bluegrass and smooth brome were more abundant in areas closer to roads. Kentucky bluegrass was also more abundant in areas farther from trails and those historically grazed by cattle. Our research demonstrates that the effect of landscape disturbances on exotic grasses varies between species and suggests that patterns of invasion may reflect different introduction histories. Given our findings, conserving the integrity of northern fescue prairies should account for human disturbances of landscapes and their effects on the proliferation of exotic plants into areas of native prairie.

Desiccation following prolonged air exposure challenges survival of aquatic plants during droughts, water drawdowns, and overland dispersal. To improve predictions of plant response to air exposure, we observed the viability of vegetative fragments of 10 aquatic plant species (Cabomba caroliniana, Ceratophyllum demersum, Elodea canadensis, Egeria densa, Myriophyllum aquaticum, Myriophyllum heterophyllum, Myriophyllum spicatum, Potamogeton crispus, Potamogeton richardsonii, and Hydrilla verticillata) following desiccation. We recorded mass loss, desiccation rate, and plant fragment survival across a range of air exposures. Mass loss accurately predicted viability of aquatic plant fragments upon reintroduction to water. However, similar periods of air exposure differentially affected viability between species. Understanding viability following desiccation can contribute to predicting dispersal, improving eradication protocols, and disposing of aquatic plants following removal from invaded lakes or contaminated equipment.

The continued threat of an invasive, annual brome (Bromus) species in the western United States has created the need for integrated approaches to postfire restoration. Additionally, the high germination rate, high seed production, and seed bank carryover of annual bromes points to the need to assay soil seed banks as part of monitoring programs. We sampled the soil seed bank to help assess the effectiveness of treatments utilizing the herbicide Plateau® (imazapic) and a perennial native seed mix to control annual Bromus species and enhance perennial native plant establishment following a wildfire in Zion National Park, Utah. This study is one of few that have monitored the effects of imazapic and native seeding on a soil seed bank community and the only one that we know of that has done so in a pinyon–juniper woodland. The study made use of untreated, replicated controls, which is not common for seed bank studies. One year posttreatment, Bromus was significantly reduced in plots sprayed with herbicide. By the second year posttreatment, the effects of imazapic were less evident and convergence with the controls was evident. Emergence of seeded species was low for the duration of the study. Dry conditions and possible interactions with imazapic probably contributed to the lack of emergence of seeded native species. The perennial grass sand dropseed outperformed the other species included in the seed mix. We also examined how the treatments affected the soil seed bank community as a whole. We found evidence that the herbicide was reducing several native annual forbs and one nonnative annual forb. However, overall effects on the community were not significant. The results of our study were similar to what others have found in that imazapic is effective in providing a short-term reduction in Bromus density, although it can impact emergence of nontarget species.

Understanding the ecological distribution range of exotic trees in an arboreal ecosystem is essential to managing natural forest resources sustainably. Forest resource mapping can be applied as a powerful tool in the identification of forest resource threat patterns, and in monitoring ongoing changes associated with a landscape. This study offers an insight on Russian-olive and its impact on a spatially bound ecosystem, namely, Bismarck–Mandan Wildland–Urban Interface (BMWUI). Data from the National Agricultural Imagery Program collected in 2005 and 2010 and in situ reference data were used to estimate the potential habitat of Russian-olive using ArcGIS ArcInfo® 9.3 (ESRI, Redlands, CA). Russian-olive plants are discernible on aerial photographs with a fine spatial resolution because of silvery gray-green leaves in the upper strata of their canopies. Results showed that Russian-olive occupied 110 ha (272 acres) in BMWUI in 2005 and of that, 13 ha (12%) was in inundated habitats. In addition, Russian-olive in 2010 covered 125 ha within the BMWUI and of that, 25 ha (20%) was in inundated habitats. Russian-olive showed a close association with the silt loam and silty clay soil type, which occurs along the Missouri River floodplain. Our findings revealed that the species is well established in riparian habitats and other open habitats such as roadside and agricultural lands. There is a greater likelihood of lateral spread of Russian-olive throughout the BMWUI that may require active management to avert undesirable conservation impacts.

The common reed (Phragmites australis) is one of the most invasive vascular plants in northeastern North America. A competitive genotype from Eurasia has recently invaded road and agricultural ditches, which facilitate the dispersal of the plant over long distances. However, large tracts of roadsides—apparently propitious for the establishment of the plant—are not invaded by the grass. We hypothesized that the absence of the invader is associated with physical and biological characteristics of roadsides. To test this hypothesis, we collected field data and developed two statistical models to explain the presence or absence of the common reed along a highway of southern Quebec highly invaded by the plant but with contrasting patterns of common reed distribution. The models explained 23 to 30% of the total variance and correctly predicted the presence or absence of common reed 71% of the time. The models suggest that a dense woody plant cover over a drainage ditch limits the establishment and/or expansion of the common reed, probably by competition for light and space. Also, shaded ditches are not subjected to a frequent maintenance, and are therefore less disturbed, probably further reducing common reed invasion because the germination of their seeds is less likely without soil disturbance. This study yields insights on the potential of woody plants for controlling the expansion of invasive grasses, and could help to justify the preservation of dense shrubs and tree hedges along right-of-ways.

Desert grasslands of the southwestern United States have experienced an increase in the abundance and distribution of woody plant species over the past century. Shrub encroachment has caused a substantial loss of grasslands in the northern Chihuahuan Desert. The Chihuahuan Desert has also been invaded by Lehmann lovegrass, a fire-adapted species from southern Africa. In 1999, the U.S. Department of Agriculture–Agricultural Research Service burned a remnant desert grassland to determine the effects of prescribed fire on shrub–perennial grass dynamics. The grassland also contained the nonnative perennial grass Lehmann lovegrass. I am reporting on a study that was initiated to determine whether prescribed burning would alter the vegetative community within and proximal to a Lehmann lovegrass–dominated patch. Cover of Lehmann lovegrass showed no significant response to the burn treatment. Of the dominant native species, only black grama and broom snakeweed had a significant year by treatment interaction. No species or growth form had a significant vegetation type by year by treatment interaction. After 6 yr, differences between burned and unburned transects were not significant for any species or growth form.

Common reed (Phragmites australis) is a nonnative invasive perennial grass that is problematic in aquatic and riparian environments across the United States. Common reed often forms monotypic stands that displace native vegetation which provide food and cover for wildlife. To help maintain native habitats and manage populations of common reed in the United States, an understanding of its life history and starch allocation patterns are needed. Monthly biomass samples were harvested from sites throughout the Mobile River delta in southern Alabama, USA from January 2006 to December 2007 to quantify seasonal biomass and starch allocation patterns. Total biomass of common reed throughout the study was between 1375 and 3718 g m−2 depending on the season. Maximum aboveground biomass was 2200 ± 220 g m−2 in October of 2006 and 1302 ± 88 g m−2 in December of 2007. Maximum belowground biomass was seen in November of 2006 and 2007 with 1602 ± 233 and 1610 ± 517 g m−2 respectively. Biomass was related to ambient temperature, in that, as temperature decreased aboveground biomass (p = 0.05) decreased. Decreases in aboveground biomass were followed by an increase in belowground biomass (p < 0.01). Starch comprised 1 to 10% of aboveground biomass with peak temporary storage occurring in July and August 2006 and September to November of 2007. Belowground tissues stored the majority of starch for common reed regardless of the time of year. Overall, belowground tissues stored 5 to 20% of total starch for common reed with peak storage occurring in December 2006 and October 2007. Starch allocation to belowground tissues increased as temperatures decreased. Understanding seasonal life history patterns can provide information to guide management strategies by identifying the vulnerable points in biomass and starch reserves in common reed.