Dutch Friesian purebred and Holstein Friesian-Dutch Friesian crossbred veal calves were used to study effects of genotype and feeding level on carcass composition. Carcass analyses were carried out on a total of 56 calves of two genotypes. Calves were reared for 22 weeks and given food dependent on age. From 1 to 16 weeks calves were given the same amount of milk replacer. At week 16, calves were assigned to either a high or low feeding level. Total energy intakes for the two groups were 5062 MJ metabolizable energy (ME) and 5408 MJ ME, respectively. Carcass weight and carcass composition traits showed no significant interaction between genotype and feeding level. Dutch Friesians had higher lean: bone ratios than Holstein Friesian crossbreds (3·85 v. 3·67). Differences between the two genotypes for fat and lean proportions in the carcass were not significant. Average proportions for lean and fat were 653 and 134 g/kg.

Differences between the two feeding levels for carcass composition were significant. Calves on the low feeding level had 655 g lean and 131 g fat per kg, while calves on the high level had 645 g lean and 143 g fat per kg.

The influence of the dissection technique on the differences in lean and muscle proportion between groups was studied on 18 carcasses using a standardized butcher's method (the Institute of Animal Husbandry (IVO) standard method) and the European Economic Community (EEC) reference method for beef. Average proportion of lean was 640 g/kg by the IVO method and of muscle was 557 g/kg by the EEC method. The difference between the two genotypes for lean proportion was smaller by the' EEC method due to the more complete separation between muscle and fatty tissue. Dutch Friesians had more intermuscular fat than Holstein Friesian crossbreds. Results of the EEC reference method indicate differences between the two feeding levels for fat as well as for protein deposition.

An investigation was made of the association of transferrin types with birth weight, weaning weight and average daily gain from birth to weaning in three breeds of Iranian sheep. Transferrin type was not a significant source of variation in weight.

In 1968-69 for a mean period of 101 days before lambing, 72 cross-bred ewes, and in 1969-70 for a mean period of 88 days, 107 ewes were given roughage daily in the form of (a) 0·91 kg field-cured hay (b) 0·45 kg of hay plus 0·23 kg dried sugar-beet pulp nuts, or (c) 0·45 kg of pulp nuts. Each diet was supplemented by a concentrate allowance. From the 6th week before lambing was due to start, this allowance was progressively and uniformly increased.

There was no evidence that the dried sugar-beet pulp had adversely affected performance in terms of ewe body-weight change, lamb numbers, litter weight at birth or 4 weeks of age, fleece weight or general health. It is therefore concluded that dried sugar-beet pulp can be used safely as a replacement for hay in supplying up to 67% of the energy needed for maintenance by ewes.

A by-product of the distilling industry designated ‘distillers’ grains plus solubles' was evaluated using forty-eight growing pigs. The product was included at 0, 14·7 and 25% in three diets in which the proportion of other constituents was adjusted to give similar concentrations of TDN, dry matter and crude protein.

The performance and carcass quality of pigs receiving 0% and 14·7% of the by-product in the diet was similar, but inclusion at 25% significantly reduced growth rate (g/day) from 632 for the 0% diet to 578.

Two experiments were conducted to investigate the requirement for dietary crude protein (CP) in growing blue-breasted quail (BBQ). In Experiment 1, 300 1-day-old quails were randomly assigned to 10 groups according to a 2 × 5 factorial arrangement of treatments with two metabolisable energy (ME) levels (12.13 and 13.39 MJ/kg) and five CP concentrations (160, 190, 220, 250 and 280 g/kg) for 8 weeks. In Experiment 2, 300 1-day-old quails were subjected to a different factorial arrangement of treatments with two ME levels (11.51 and 12.13 MJ/kg) and five CP concentrations (210, 220, 230, 240 and 250 g/kg) for 28 days. Experiment 1 revealed that an interaction existed in weight gain between ME and CP levels in weeks 1 to 4. In both ME groups, quails receiving CP of 160 g/kg showed the least weight gains (P < 0.05). No differences (P > 0.05) existed in weight gain between the ME groups in which quails ingested CP of 250 and 280 g/kg, whereas quails consuming CP of 220 g/kg with an ME of 13.39 MJ/kg had smaller weight gain than did those ingesting higher CP concentrations (P < 0.05). Of main effects for weeks 1–4, quails treated with an ME of 12.13 MJ/kg consumed more feed than did those receiving another ME level, whereas quails in both ME treatments showed similar feed efficiencies. For weeks 5 to 8, no difference (P > 0.05) in weight gain, feed intake and feed efficiency was seen regardless of ME levels, and no interaction existed between ME and CP levels. In Experiment 2, the best weight gain and feed efficiency were achieved when the dietary CP concentration was more than 210 g/kg, and quails treated with 11.51 MJ/kg showed better weight gain and feed efficiency (P < 0.05) than did those that received 12.13 MJ/kg. Furthermore, the weight gains and protein intakes on the basis of per MJ from the two experiments were pooled together to estimate the protein intake necessary for the best growth performance by two mathematic models; they were then converted to dietary CP concentrations of 204 (minimum) and 233 g/kg (maximum) when ME was 11.51 MJ/kg. In conclusion, BBQ will achieve good growth performance with dietary CP of more than 204 g/kg on the basis of an ME of 11.51 MJ/kg in weeks 1 to 4.

Two pairs of uniformly treated identical twins were weighed four times daily for 16 days. In addition, daily growth rate was analysed for 10 pairs of identical twins kept on high and low planes of nutrition over a 16-week period.

Identical twins fluctuated in body weight from hour to hour and day to day in a significantly more similar manner than did unrelated animals. The greater similarity found when all animals were uniformly treated still persisted when identical twins were placed on different planes of nutrition.

The Slaughter of Animals (Humane conditions) Act 1990 prohibits killing animals within sight of another. There is a need to produce scientific evidence to show whether the sight of sticking (exsanguination) is stressful to animals that happen to observe this procedure. This has important consequences on the design of stunning and sticking areas in the abattoir. Sticking in a separate are can lead to a delay between stunning and sticking and increase the chance of animals regaining consciousness.

In this study experiments were carried out to determine whether sheep did responded in a way which could suggest they found the experience of witnessing sticking distressing.

The clearance rate of digesta from the reticulo-rumen may limit feed intake and thus production in ruminants. The longer the retention time in the rumen the better the cell wall carbohydrates are digested. However, a fast rate of passage of indigestible feed particles to the lower digestive tract is essential for maximal feed intake. The feeding level, type of diet and the physical characteristics of the feed particles affect the rate of passage from the rumen. The rate of passage has mostly been determined by external marker techniques. Because of physico-chemical characteristics these markers do not always accurately represent the behaviour of feed.particles. An alternative method, the rumen evacuation technique, may describe better the kinetic characteristics of cell walls. In this study marker and rumen evacuation techniques were compared in the determination of the passage rate and retention times.

Pregnant sows in poor body condition require a high feed intake to restore body fat reserves. However, a high intake in the period following conception may increase embryo losses. The first objective of this experiment was to determine whether a high feed allowance after conception has any effect on litter size compared with a normal allowance. The second objective was to investigate the effects of body condition at conception and feed rate in early pregnancy on the components of maternal gain.

Multiparous cross-bred sows of Large White and Landrace lineage were classified subjectively at farrowing according to body condition as either fat (F) or thin (T). A single diet containing 12.5 MJ DE/kg and 150 g CP/kg was fed throughout the experiment. The fat sows were fed more generously than the thin sows during the 28 day lactation with mean intakes of 5.3 and 4.8 kg/day. This was to maintain the differential in body condition between the two groups. After weaning all sows were fed 3 kg per day until mating. Within the fat and thin groups sows were allocated at random to either 4 or 2 kg of feed per day for 30 days after mating.

In a paper of this kind it would be very easy to be over ambitious and therefore be tempted to go too far in an effort to establish relationships between the physical and the other factors which have influenced the livestock policy of this region. Apart from the complexities of these relationships which make it difficult to determine their influence, there is generally a large gap in our knowledge of the physical data relating to livestock farming. For example it is only fairly recently that we have come to realise the subtle influence that certain “trace elements” have on the health of ruminant livestock, and also the marked differences that exist in the feeding value of grass of varied species according to the type of farming practised.

Man-animal relationships are involved in the process of fox domestication. Handling being an important part of man-animal contacts, causes stress responses in farm-bred blue foxes. The purpose of this study was to determine how prenatal stress induced by handling pregnant vixens influences certain morphometric and hormonal parameters of adrenocortical and gonadal function in the prepubertal offspring. Blue fox females were subjected to daily handling sessions, each of 1 min, in the last trimester of pregnancy (term = 52 days). Plasma concentrations of ACTH, cortisol, progesterone, oestradiol and testosterone, as well as the in vitro adrenal and gonadal production of steroids were measured by radio-immunoassay in control (C, no. = 56) and prenatally stressed (PS, no. = 56) blue fox cubs of both sexes at the age of 6 to 7 months. Prenatal stress decreased plasma concentration of cortisol (C: 31·0 (s.e. 4·3) v. PS: 22·7(s.e. 1·6) ng/ml, P < 0·05) as well as progesterone (C: 1·00(s.e. 0·10) v. PS: 0·65(s.e. 0·05) ng/ml, P < 0·05) in female cubs. Prenatal stress did not cause any changes in adrenal or gonadal weights, plasma concentrations of testosterone or oestradiol, or in vitro adrenal or gonadal steroid production, in either sex. It is concluded that persistent handling of pregnant blue foxes did not affect the prepubertal development of the reproductive system but resulted in disregulation in the hypothalamic-pituitary-adrenal axis in the female offspring.

Conditioned taste aversion (CTA) is a useful tool to modify animal feed preferences, allowing the implementation of selective grazing to control weeds in tree orchards without damaging the trees or affecting fruit production. LiCl is commonly used for inducing CTA. However, studies investigating the long-term persistence of CTA by LiCl in small ruminants are scarce. With this aim, we evaluated the efficiency of two LiCl doses (AV1 and AV2, 175 and 200 mg/kg BW, respectively) and a control (C, 0 mg/kg BW) for averting non-lactating dairy goats (n=15) to olive tree leaves. Aversion induction was reinforced on day 9 in those goats that consumed >10 g of olive leaves. Mid-term aversion effectiveness was assessed by five double-choice feeding tests (days 16, 24, 31, 38 and 53) of 30 min each, where 100 g of olive leaves were offered side-by-side with 390 g of Italian rye-grass (as-fed). Long-term aversion effectiveness was assessed in C, AV1 and AV2 goats by grazing for 30 min in paddocks with a simulated olive tree (days 59, 90, 121, 182 and 420). Moreover, C and AV2 goats were compared under on-field conditions (days 143, 211 and 363) in a commercial olive grove also for 30 min. The CTA proved to be established with a single LiCl dose in all goats and persisted for 4 and 55 days in AV1 and AV2 goats, respectively (P<0.001). However, 80% AV1 and 20% AV2 goats needed to be reinforced at day 9. When grazing under simulated olive tree and commercial olive grove conditions, the CTA goats, especially AV2 group, avoided the contact with the olive trees and minimally used a bipedal stance to feed leaves, than control goats. On average, time proportion spent consuming olive leaves and sprouts was much greater (P<0.05) for C (50.7±9.1%) than for AV1 (14.4±3.9%) and AV2 (3.1±0.9%). In conclusion, the 200 mg LiCl/kg BW dose was more effective than the 175 mg LiCl/kg BW dose for inducing an effective long-term CTA to olive tree leaves in goats.

The origins and phylogeny of different sheep breeds has been widely studied using polymorphisms within the mitochondrial hypervariable region. However, little is known about the mitochondrial DNA (mtDNA) content and phylogeny based on mtDNA protein-coding genes. In this study, we assessed the phylogeny and copy number of the mtDNA in eight indigenous (population size, n=184) and three introduced (n=66) sheep breeds in China based on five mitochondrial coding genes (COX1, COX2, ATP8, ATP6 and COX3). The mean haplotype and nucleotide diversities were 0.944 and 0.00322, respectively. We identified a correlation between the lineages distribution and the genetic distance, whereby Valley-type Tibetan sheep had a closer genetic relationship with introduced breeds (Dorper, Poll Dorset and Suffolk) than with other indigenous breeds. Similarly, the Median-joining profile of haplotypes revealed the distribution of clusters according to genetic differences. Moreover, copy number analysis based on the five mitochondrial coding genes was affected by the genetic distance combining with genetic phylogeny; we also identified obvious non-synonymous mutations in ATP6 between the different levels of copy number expressions. These results imply that differences in mitogenomic compositions resulting from geographical separation lead to differences in mitochondrial function.

Eight wether lambs were used to determine effects of an oestradiol-17β (E2β) implant on metabolic responses to an epinephrine challenge. Blood was sampled before and after an epinephrine challenge, on days 0 and 22 of E2p implantation.Oestradiol-17 ft implants increased pre-injection (basal) serum insulin (INS), growth hormone and free fatty acids (FFA), and decreased basal serum urea nitrogen. After 21 days of E2Pimplantation, total concentrations of FFA decreased and INS increased in response to the epinephrine challenge compared with pre-implantation values. It is concluded that E2/3 implants alter metabolic responses to an epinephrine challenge in wether lambs.

Two field trials were conducted with 138 and 123 bacon-weight pigs respectively from one farm to determine the effect on bacon yield of feeding the animals sugar solutions prior to slaughter. Three treatments were tested: immediate slaughter on arrival at the abattoir; lairage overnight with access to water only (15 h); and overnight lairage with access to a sugar solution (6 h) followed by water only (9 h) prior to slaughter. A glucose syrup solution was used in one trial and a sucrose solution of similar hexose-equivalent concentration (550 g/kg) in the other. Access to either of the sugar solutions, relative to water only or immediate slaughter, increased bacon yield. This was entirely due to glucose-fed pigs having a higher carcass yield (2·3 % and 1·5 % respectively) and a trend in this direction for the sucrose-fed animals (0·9% and 1·0% respectively). Carcasses of glucose-fed pigs actually gained less weight than those of their counterparts during the curing process, but this did not occur with the sucrose solution. Access to sucrose relative to water only and immediate slaughter increased liver weights by 32% and 42% respectively. Lower hepatic responses were obtained with glucose syrup (18% and 12%). Both sugar solutions reduced muscle ultimate pH (0·1 to 0·2 unit) relative to water only in overnight lairage.

Few data are available to evaluate the Metabolisable Protein (MP) System (AFRC, 1992) as an aid to ration formulation for dairy cows. Responses of lactating dairy cows to MP were evaluated in two experiments.

In Experiment One, groups of 10 Holstein cows were offered grass silage ad libitum and 7kg DM/d of one of six (treatments 1-6) compound foods from 39d after calving, for eight weeks. All rations were isoenergetic, assuming silage intake of 11kg DM/d (typical for the herd). Ratios between MP supply (calculated from in situ protein degradability measurements) and requirement (calculated for a standard cow: 565kg live weight, 281 milk/d, 40g/kg butterfat, 33g/kg milk protein, liveweight change =0kg/d) were 1.00, 1.02, 1.06, 1.06, 1.1 and 1.17 for treatments 1-6, respectively.

It has previously been shown (Robinson et al., 1985) that the normal breeding season of Greyface and Scottish Blackface ewes can be advanced by the daily administration of the indoleamine melatonin; the administration of the melatonin beginning midway during the anoestrous period. If melatonin is administered towards the end of the normal breeding season, oestrous activity can be extended, but only for a period of about six weeks until the ewe becomes refractory to the melatonin treatment (Nett and Niswender, 1981). There is no information on the administration of melatonin at the beginning of the anoestrous period.

In the following experiments we report results for the influence on the subsequent breeding activity of Greyface ewes of the oral administration of melatonin at the beginning of their normal period of anoestrus. We also report the results of an investigation on the effects of melatonin in advancing the breeding season of Scottish Blackface ewes maintained on either a high or low plane of nutrition. The experiments were carried out under natural daylength conditions at 57°N.