Parrots are popular companion animals but show prevalent and at times severe welfare issues. Nonetheless, there are no scientific tools available to assess parrot welfare. The aim of this systematic review was to identify valid and feasible outcome measures that could be used as welfare indicators for companion parrots. From 1,848 peer-reviewed studies retrieved, 98 met our inclusion and exclusion criteria (e.g. experimental studies, captive parrots). For each outcome collected, validity was assessed based on the statistical significance reported by the authors, as other validity parameters were rarely provided for evaluation. Feasibility was assigned by considering the need for specific instruments, veterinary-level expertise or handling the parrot. A total of 1,512 outcomes were evaluated, of which 572 had a significant P-value and were considered feasible. These included changes in behaviour (e.g. activity level, social interactions, exploration), body measurements (e.g. body weight, plumage condition) and abnormal behaviours, amongst others. Many physical and physiological parameters were identified that either require experimental validation, or veterinary-level skills and expertise, limiting their potential use by parrot owners themselves. Moreover, a high risk of bias undermined the internal validity of these outcomes, while a strong taxonomic bias, a predominance of studies on parrots in laboratories, and an underrepresentation of companion parrots jeopardised their external validity. These results provide a promising starting point for validating a set of welfare indicators in parrots.

The modern idea of purebred dogs has come under increasing critical scrutiny over recent decades. In light of this critical focus and other developments in society, some new trends in how companion dogs are bred and acquired have emerged. This means a diminishing influence from traditional kennel clubs with more dogs being sold without a pedigree, stricter legal restrictions on dog breeding, growing popularity of deliberate crosses of established breeds (i.e. so-called designer breeds) and growing hype around the benefits of mixed-breed dogs. We give an overview of these trends and discuss to what extent they will serve to promote dogs that are innately healthy, have good welfare and function well in their various roles in today’s world. We argue that newly invented designer breeds and mixed breeds also have worrying health and behavioural problems, and that the predictability of purebred dogs with respect to body size, basic behaviours, known need for grooming, disorder profiles and other attributes may well offer some benefits for a satisfying human-dog relationship seen from both sides. The optimal future seems to lie in the middle ground, where the future organised dog world (i.e. kennel and breed clubs or their successor organisations) will need to re-open the breed registries, remove wording from breed standards that currently promotes extreme conformation, support selection against disease-predisposing genotypes and phenotypes and refocus dog showing and breeding to promote health and appropriate behaviour.

The capture of wild-living animals can provide valuable information that is critical in developing and implementing effective conservation actions. These capture procedures, however, often require direct handling of individuals by researchers, and conservationists should constantly seek to improve capture methods so that the impacts on animal welfare are minimised. The ngwayir (western ringtail possum; Pseudocheirus occidentalis) is a critically endangered arboreal marsupial in need of effective conservation. It is, however, not amenable to conventional trapping, leading to the use of methods such as nest robbing and tranquilisation using dart guns or pole syringes, which involve potentially serious animal welfare risks and longer exposure of animals to humans as compared to conventional trapping. In pursuit of an improved capture method, we investigated opportunistically whether placing traps above the ground would increase the capture success rate of the species, using wire cage traps baited with universal bait and fruit. Between 2010 and 2019, we deployed trapping grids in Locke Nature Reserve and adjacent campsites near Busselton, WA, Australia, with traps placed on the ground for 1,985 trap nights and traps placed on horizontal tree branches, fallen trees or fences, 1–2 m above ground for 694 trap nights. With the above ground traps we trapped 82 ngwayirs out of 694 trap nights, 27 in autumn and 55 in spring. We also captured eleven common brushtail possums (Trichosurus vulpecula; 1.6% trap success rate), 12 King’s skinks (Egernia kingii; 1.7%) and five black rats (Rattus rattus; 0.7%). Trapping success rate was higher in elevated traps (up to 18.3%) compared to traps on the ground (0.5%) and using fruit as bait increased the trap success rate. These results suggest that using elevated traps baited with fruit is a practical, effective method to capture the ngwayir.

In-farm livestock production vaccinations are commonly delivered intramuscularly using needles. While there are alternative strategies these have been subject to little attention and limited commercialisation. One such alternative is needle-free vaccines and studies have focused on the immune response few have addressed the welfare implications. This study aims to compare the impact of intradermal needle-free vaccination and intramuscular injection in terms of the welfare of the piglets. A total of 179 piglets were divided into two treatments: intradermal needle-free delivery and intramuscular delivery of a vaccine. Measures of health and welfare included, vocalisations, behavioural observations, papule formation, and weight. Piglets vaccinated via the needle-free intradermal route vocalised less and displayed no significant behavioural differences but showed increased weight compared to piglets vaccinated intramuscularly. The use of a needle-free device to deliver a vaccine through an intradermal route revealed no adverse effects on piglet welfare and supports the use of alternative strategies to vaccinate livestock.

The aim of our study was to assess the nest-building behaviour of two mouse (Mus musculus) strains using different nesting materials and examine possible sex- and housing-specific effects. Adult mice of two strains (C57BL/6J; n = 64 and BALB/cAnNCrl; n = 99) were randomly allocated to the following housing groups: single-housed male, single-housed female, pair-housed male and pair-housed female. One of the following nest-building materials was placed in each home-cage in a random order: nestlets (Plexx BV, The Netherlands), cocoons (Carfil, Belgium), wooden wool, crinklets and compact (all three, Safe, Germany). The following day, nests were rated applying a nest-scoring scale ranging from 0 to 10, the nests were removed, and a different nest-building material provided. In both tested strains, nestlets achieved the highest nest-building scores when compared to the other four nest-building materials. All nest-building materials scored higher in BALB/c mice compared to C57BL/6J animals reaching statistical significance in crinklets only. Sex comparison revealed that female C57BL/6J mice only scored significantly higher using crinklets than males and BALB/c female mice were rated significantly higher using wooden wool, cocoons and compact than their male counterparts. While pair-housed C57BL/6J animals built higher-rated nests than single-housed mice in the C57BL/6J strain in all five materials tested, the scores were not significantly different in the BALB/c strain. Results of the present study reveal significant strain-, sex- and housing-related influences on the complexity of nests using different standardised building materials. Such observations need to be taken into account when planning the optimal enrichment programme for laboratory animals.

Animal health is only a part of animal welfare but still a very important part, and one which has been firmly brought home to livestock industries in recent years with the re-emergence of diseases such as avian influenza, foot and mouth and, more recently, bluetongue.

Stunning with carbon dioxide gas (CO2) is used in turkeys (Meleagris gallopavo var domesticus) as an alternative to electrical water bath stunning. Investigations were carried out to assess the behavioural and clinical responses of turkeys stunned in a V-shaped CO2 tunnel while sitting in their transport crates as part of the usual slaughter routine in a commercial abattoir. The CO2 concentration in the tunnel rose from 27% at the first observation point (A) to 74% at point D and the transport time was 180 s. At window 1, 37.1% of the birds showed head shaking, 3.7% deep breaths and 2.9% intensive wing-flapping and at window 2 the respective figures were 2.2, 18.4 and 6.2%. All birds appeared to have lost consciousness at the point of leaving the tunnel. Prior to shackling, 15 s after leaving the tunnel, 230 animals were tested for both interphalangeal reflex and eyelid closure; 20.4% had an incomplete eyelid closure but none displayed an interphalangeal reflex. It would appear that the tunnel system we investigated stuns turkeys effectively within 180 s. However, the initial stunning phase of 40-105 s appears to cause the animals distress, which is demonstrated by head shaking, deep breaths and wing flapping.

To define suitable holding conditions for fish used in experimental studies on food consumption and growth, the effect of cover on growth at two ration levels was quantified for immature individuals of two common, freshwater species: the threespine stickleback (Gasterosteus aculeatus: Gasterosteidae) and the European minnow (Phoxinus phoxinus: Cyprinidae). In the European minnow, the presence of cover significantly increased growth rates and also the rate of food consumption at ad libitum rations; however, cover had no significant effect on the growth of the threespine stickleback. In both species, fish fed ad libitum rations had higher growth rates than fish fed 4% of body weight per day. This study illustrates the need for species specific holding conditions and illustrates an approach to welfare using measurements of traits closely related to fitness.

Many animal preference experiments involve test stimuli that have been chosen by the experimenter to represent different strengths of a single attribute. It is assumed that the animals also scale the test stimuli along a single dimension. This paper shows how it is possible to use the ‘Unfolding Technique' developed by Coombs (1964) to check the validity of this assumption. A simple experiment is described which used Coombs' technique to verify that three visual test stimuli were ranked by laboratory rats along a single dimension. These stimuli were subsequently used in an experiment to see how different housing conditions changed rats' preferences for visual complexity.

For management and/or research purposes, chimpanzee mothers and their offspring are often physically separated from one another at an earlier age than they would be in the wild. Studies of the behavioural and physiological effects of mother-infant separation on infant behaviour have been conducted in both human and nonhuman primates. However, few studies have been conducted to examine the response of the mother to separation from her infant, particularly in great apes. The current study opportunistically examined the response of twelve chimpanzee mothers to separation from 15 of their offspring ranging from 1.8 to 5.4 years of age. Behavioural data (proximity of the mother to other group members, and fifteen behaviours representing six categories [agonistic, prosocial, vocalisations, abnormal, locomotor, inactive]) were collected for three weeks prior to and three to five weeks following the permanent removal of the offspring from their mothers. A repeated measures MANOVA conducted on all subjects revealed no significant change in behaviour following separation. There was a significant increase in inactivity following separation of the offspring (F2.21 = 5.47, P < 0.05) in a subgroup of mothers (n = 8) that was studied more intensively on the first day of separation. Maternal age, infant age, presence of other offspring, and past experience with mother-offspring separation had no effect on response to separation. These results contradict those of mother-infant separation studies in monkeys and indicate that most behavioural indicators of well-being are stable in chimpanzee mothers that remain in their familiar environment and social group following offspring separation.

Post-racing thoroughbreds (TBs) are increasingly being considered for Equine Assisted Services (EAS), but their use has not yet been widely characterised. This study aimed to generate detailed data on TBs and other breeds (OBs) of horses in EAS via an online survey. The survey was completed by 129 EAS practitioners from 15 countries and reported detailed data on 427 EAS equids, including 57 TBs. Most of the EAS horses were housed collectively, had access to free exercise over 12 h per day and had unrestricted access to forage. The most commonly recorded selection criteria for EAS horses consisted of: demonstrating a good personality; enjoying the work; absence of propensity to kick/bite. Detailed data gathered on individual horses showed that in comparison to OBs, TBs were younger, more likely to be a gelding, less likely to be used in ridden programmes, and tended to present more behavioural issues than OBs. The majority of the participants agreed that TBs have specific assets of particular interest to EAS programmes, such as sensitivity, body/movement characteristics or responsiveness/flight response. Finally, a large majority of participants reported that they believe TBs to be suitable for EAS programmes and some would consider working with them. These results showed TBs to already be in use in various EAS programmes and more could be incorporated in the future. In terms of animal welfare and beneficiaries’ safety, a selection process could therefore be designed and implemented to choose the most adapted horses for each EAS centre, according to living conditions and EAS activities practised (ridden or not ridden).