Direct accelerator mass spectrometry (AMS) dating is crucial for a correct integration of plant remains in the (pre)history of crops, particularly for those that do not belong to the Neolithic package and are known to arrive in Europe much later. This paper reviews one of the earliest records of rye from Romania. The grains were discovered in the tell settlement of Cunești, which belongs to the Gumelnița communities (ca. 4600–3900 BC). In 1954, due to Danube flooding, a large portion of the south part of the tell collapsed, and between the burnt dwelling visible in the resulting profile, a large number of sherds from three typical Gumelnița pots were identified. According to the excavation’s author, rye grains were found in association with those sherds, and it was assumed that a batch was stored in these Eneolithic vessels. Consequently, the rye was published as belonging to the Gumelnița period. Our reanalysis led to two radiocarbon (14C) dates, from two different laboratories, which indicate that the Cunești rye is not prehistoric but dates to the medieval period. To correct this error concerning this rye batch and the implications for European archaeology, we decided to republish these grains in an updated chronological framework. In addition, we performed stable isotope analyses on the charred grains, confirming they were cultivated on dry land, as well as a 3D morphometric investigation. Our research brings new and original data on rye cultures from the medieval period in southeastern Europe.

Adoption of cover crops in arid agroecosystems has been slow due to concerns regarding limited water resources and possible soil moisture depletion. In irrigated organic systems, potential ecosystem services from cover crops also must be considered in light of the concerns for water conservation. A constructive balance could be achieved with fall-sown small grain cover crops; however, their impacts on irrigated organic systems are poorly understood. Our first objective was to determine the ability of fall-sown small grains [cereal rye (Secale cereale L), winter wheat (Triticum aestivum L.), barley (Hordeum vulgare L.) and oat (Avena sativa L.)] to suppress winter weeds in an irrigated, organic transition field in the southwestern USA. Small grains were planted following the legume sesbania (Sesbania exaltata (Raf.) Rydb. ex A.W. Hill) during Fall 2012 and Fall 2013. In Spring 2013 and 2014, weed densities and biomass were determined within each cover crop treatment and compared against unplanted controls. Results indicated that both barley and oat were effective in suppressing winter weeds. Our second objective was to compare weed suppression and soil moisture levels among seven barley varieties developed in the western United States. Barley varieties (‘Arivat’, ‘Hayes Beardless’, ‘P919’, ‘Robust’, ‘UC603’, ‘UC937’, ‘Washford Beardless’) were fall-sown in replicated strip plots in Fall 2016. Weed densities were measured in Spring 2017 and volumetric soil moisture near the soil surface (5.1 cm depth) was measured at time intervals beginning in December 2016 and ending in March 2017. With the exception of ‘UC937’, barley varieties caused marked reductions in weed density in comparison with the unplanted control. Soil moisture content for the unplanted control was consistently lower than soil moisture contents for barley plots. Barley variety did not influence volumetric soil moisture. During the 2017–2018 growing season, we re-examined three barley varieties considered most amenable to the cropping system requirements (‘Robust’, ‘UC603’, ‘P919’), and these varieties were again found to support few weeds (≤ 5.0 weeds m−2). We conclude that several organically certified barley varieties could fill the need for a ‘non-thirsty’ cover crop that suppresses winter weeds in irrigated organic systems in the southwestern United States.

Cover crop residues are not widely used for weed control because, as a stand-alone tactic, they do not effectively suppress all weeds and their duration of weed control is too short. Field experiments were conducted in 1995 and 1996, under both irrigated and rainfed conditions, to quantify Amaranthus spp., Setaria spp., and soybean emergence and growth in residues of fall-planted, spring-killed barley, rye, triticale, wheat, and hairy vetch. For both weed species, seedling emergence was reduced 3 wk after soybean planting by rye and wheat residues (≥ 2, 170 kg ha−1) in 1996. In 1996, Amaranthus spp. canopy volume was reduced 38 to 71% by residues 3 wk after planting. Likewise, Setaria spp. canopy biomass was reduced 37 to 97% in residues 5 wk after planting over both years. The response comparison index was used to identify frequency by which weed growth was placed at a disadvantage relative to soybean growth. Amaranthus spp. and Setaria spp. growth suppressions 3 to 5 wk after planting indicate potential times for intervention with other integrated weed management tactics such as reduced postemergence herbicide rates and interrow cultivation.

Allelochemicals representing numerous chemical groups have been isolated from over 30 families of terrestrial and aquatic plants. Some of the compounds also have been isolated from soil in quantities sufficient to reduce plant growth. Although selected allelochemicals are believed to influence plant densities and distributions, none isolated from higher plants have been considered active enough for development as commercial herbicidal products. Almost all herbicidal allelochemicals exist in plants in nontoxic, conjugated forms. The toxic moiety may be released upon exposure to stress or upon death of the tissue. The most successful use of allelochemicals in weed control has been management of selectively toxic plant residues. For example, rye residues have controlled weeds effectively in a variety of cropping systems. Several weed species may interfere with crop growth through chemicals released from their residues. A number of noxious perennial species appear to exploit allelochemicals in their interference processes. This review focuses on the more recent chemical discoveries and how they might be exploited for weed control.

Weed management strategies are needed for no-till soybean grown on clay soils. The effect of several weed management strategies on weed biomass, soybean yield, and gross return were evaluated in 1993, 1994, and 1995 on clay soils at two locations in southern Ontario. Soybean seeds were planted in narrow (19 cm) and wide (76 cm) rows with or without a rye cover crop. Herbicide treatments included glyphosate alone, glyphosate followed by imazethapyr + metribuzin applied PRE, and glyphosate followed by acifluorfen + bentazon applied POST. Two additional treatments with interrow cultivation were included in the wide-row soybean plots with glyphosate and glyphosate + PRE treatments. A nontreated check plot without rye was also included. Presence of a cover crop did not affect weed biomass or soybean yield. The glyphosate + broadcast PRE treatment provided the most consistent weed control both in narrow- and wide-row soybean. The weed control in this treatment ranged from 92 to 100%. The other treatments provided variable weed control across years and locations. The narrow- row plots with glyphosate + broadcast PRE treatment provided the most consistent soybean yields that were generally higher than the other treatments and ranged from 2,560 to 3,420 kg/ha. Soybean yields varied across locations and years in other treatments. Similar weed control and soybean yields were obtained with banded PRE herbicide + interrow cultivation and PRE treatments; however, herbicide use was 60% lower in banded PRE herbicide + interrow cultivation treatment. Narrow-row soybean averaged 27% higher gross returns than wide-row soybean for all broadcast herbicide treatments. Narrow-row soybean with PRE herbicide provided the highest gross returns. No-till soybean in narrow rows with preplant glyphosate and broadcast PRE treatment was the most risk-efficient weed management system on clay soils.

A field study was conducted during 1997 to 2001 on a Dundee silt loam soil at Stoneville, MS, to examine the effects of rye and crimson clover residues on weeds, soil properties, soil microbial populations, and soybean yield in conventional tillage (CT) and no-tillage (NT) systems with preemergence (PRE)-only, postemergence (POST)-only, and PRE plus POST herbicide programs. Rye and crimson clover were planted in October, desiccated in April, and tilled (CT plots only) before planting soybean. Both cover-crop residues reduced density of barnyardgrass, broadleaf signalgrass, browntop millet, entireleaf morningglory, and hyssop spurge but did not affect yellow nutsedge at 7 wk after soybean planting (WAP) in the absence of herbicides. Densities of these weed species were generally lower with PRE-only, POST-only, and PRE plus POST applications than with no-herbicide treatment. Total weed dry biomass was lower when comparing CT (1,570 kg ha−1) with NT (1,970 kg ha−1), rye (1,520 kg ha−1) with crimson clover (2,050 kg ha−1), and PRE plus POST (640 kg ha−1) with PRE-only (1,870 kg ha−1) or POST-only (1,130 kg ha−1) treatments at 7 WAP. Soils with crimson clover had higher organic matter, NO3–N, SO4–S, and Mn, and lower pH compared with rye and no–cover crop soils. Total fungi and bacterial populations and fluorescein diacetate hydrolytic activity were higher in soil with crimson clover, followed by rye and no cover crop. Soybean yields were similar between CT (1,830 kg ha−1) and NT (1,960 kg ha−1), no cover crop (2,010 kg ha−1) and rye (1,900 kg ha−1), and rye and crimson clover (1,790 kg ha−1), but they were higher in PRE plus POST (2,260 kg ha−1) than in PRE-only (1,890 kg ha−1) or POST-only (1,970 kg ha−1) treatments.

The inclusion of winter cereals in spring-annual rotations in the northern Great Plains may reduce weed populations and herbicide requirements. A broad range of spring and winter cereals were compared for ability to suppress weeds and maximize grain yield at Lacombe (2002 to 2005) and Lethbridge (2003 to 2005), Alberta, Canada. High seeding rates (≥ 400 seeds/m2) were used in all years to maximize crop competitive ability. Spring cereals achieved high crop-plant densities (> 250 plants/m2) at most sites, but winter cereals had lower plant densities due to winterkill, particularly at Lethbridge in 2004. All winter cereals and spring barley were highly effective at reducing weed biomass at Lacombe for the first 3 yr of the study. Weed suppression was less consistently affected by winter cereals in the last year at Lacombe and at Lethbridge, primarily due to poor winter survival. Grain yields were highest for spring triticale and least for spring wheat at Lacombe, with winter cereals intermediate. At Lethbridge, winter cereals had higher grain yields in 2003 whereas spring cereals had higher yields in 2004 and 2005. Winter cereals were generally more effective at suppressing weed growth than spring cereals if a good crop stand was established, but overlap in weed-competitive ability among cultivars was considerable. This information will be used to enhance the sustainable production of winter and spring cereals in traditional and nontraditional agro-ecological zones.

Differential activities of BOA, DIBOA, and crude water extract of Secale cereale ‘Elbon’ were studied in culture dish bioassays using several vegetable and weed species. On average, DIBOA was about seven times more inhibitory to root growth and four times more inhibitory to shoot growth than BOA. Allelochemicals from S. cereale inhibited shoot more than root elongation of cucurbits Cucumis melo, Cucumis sativus, and Cucurbita pepo. Small-seeded crops Lycopersicon esculentum and Lactuca sativa were sensitive to S. cereale. Large-seeded crops, including the cucurbits and Zea mays var. rogusa, were tolerant. Among the small-seeded weeds Amaranthus palmeri, Digitaria sanguinalis, Echinochloa crus-galli, and Eleusine indica, E. crus-galli was least susceptible. Inhibition of germination by BOA or DIBOA occurred only in small- to medium-seeded species, including A. palmeri, D. sanguinalis, E. indica, L. sativa, L. esculentum, and Sida spinosa. Large-seeded species C. melo, C. sativus, C. melopepo, Z. mays var. rogusa, Ipomoea hederacea var. integriuscula, Ipomoea lacunosa, and Senna obtusifolia were tolerant to allelochemicals from S. cereale. This bioassay indicated a promising potential for controlling small-seeded weeds in large-seeded crops.

Whole-grain rye foods reduce appetite, insulin and sometimes glucose responses. Increased gut fermentation and plant protein may mediate the effect. The aims of the present study were to investigate whether the appetite-suppressing effects of whole-grain rye porridge could be enhanced by replacing part of the rye with fermented dietary fibre and plant protein, and to explore the role of gut fermentation on appetite and metabolic responses over 8 h. We conducted a randomised, cross-over study using two rye porridges (40 and 55 g), three 40-g rye porridges with addition of inulin:gluten (9:3; 6:6; 3:9 g) and a refined wheat bread control (55 g), served as part of complete breakfasts. A standardised lunch and an ad libitum dinner were served 4 and 8 h later, respectively. Appetite, breath hydrogen and methane, glucose, insulin and glucagon-like peptide-1 (GLP-1) responses were measured over 8 h. Twenty-one healthy men and women, aged 23–60 years, with BMI of 21–33 kg/m2 participated in this study. Before lunch, the 55-g rye porridges lowered hunger by 20 % and desire to eat by 22 % and increased fullness by 29 % compared with wheat bread (P<0·05). Breath hydrogen increased proportionally to dietary fibre content (P<0·05). Plasma glucose after lunch was 6 % lower after the 55-g rye porridges compared with wheat bread (P<0·05) and correlated to breath hydrogen (P<0·001). No differences were observed in ad libitum food intake, insulin or GLP-1. We conclude that no further increase in satiety was observed when replacing part of the rye with inulin and gluten compared with plain rye porridges.

A liquid chromatography–MS (LC-MS) metabolomics analysis of plasma from portal–arterial catheterised pigs fed breads prepared with whole-grain rye or wheat flour with added concentrated arabinoxylan (AX) or β-glucan (BG) was conducted. Comparison of the effects of concentrated fibres with whole grains has received little attention. Six female catheterised pigs were given two white wheat breads with wheat AX or oat BG, two rye breads with ground rye (GR) or intact rye kernels (RK), and a control white wheat bread (WF) on separate occasions in a randomised cross-over design. The amount of available carbohydrate was similar for the five breads but varied in the content of protein. Plasma was collected continuously for 4 h after feeding. Glucose levels in the portal vein were reduced postprandially in response to the AX, GR and RK breads that had high contents of AX compared with WF bread (P < 0·03). AX and RK breads further tended to decrease plasma levels of some lysophosphatidylcholine species (P ≤ 0·10). The abundance of amino acids in plasma correlated with the protein contents in the breads and leucine uptake significantly affected insulin secretion in the mesenteric artery. In conclusion, the present study revealed that concentrated AX in wheat bread had similar positive effects as whole-grain rye bread on glucose and lipid metabolism.

Organic cropping systems that utilize winter grown cereal–legume cover crop mixtures can increase plant available nitrogen (N) to a subsequent cash crop, but the rate of N release is uncertain due to variations in residue composition and environmental conditions. A study was conducted to evaluate N availability from rye (Secale cereale L.)–hairy vetch (Vicia villosa Roth) cover crop mixtures and to measure the response of organically grown sweet corn (Zea mays L.) to N provided by cover crop mixtures. Nitrogen availability from pure rye, pure hairy vetch, and rye–vetch mixtures was estimated using laboratory incubation with controlled temperature and soil moisture. Sweet corn N response was determined in a 2-year field experiment in western Washington with three cover crop treatments as main plots (50:50 rye–vetch seed mixture planted mid September, planted early October, and none) and four feather meal N rates as subplots (0, 56, 112 and 168 kg available N ha−1). Pure hairy vetch and a 75% rye–25% hairy vetch biomass mixture (R75V25) released similar amounts of N over 70 days in the laboratory incubation. But, the initial release of N from the (R75V25) treatment was nearly 70% lower, which may result in N release that is better timed with crop uptake. Cover crops in the field were dominated by rye and contained 34–76 kg ha−1 total N with C:N ranging from 18 to 27. Although time of planting and management of cover crop quality improved N uptake in sweet corn, cover crops provided only supplemental plant available N in this system.

Bread is an elementary part of the western diet, and especially rye bread is regarded as an important source of fibre. We investigated the heritability of eating bread in terms of choice of white and rye bread and use-frequency of bread in female and male twins in Denmark and Finland. The study cohorts included 575 Danish (age range 18–67 years) and 2009 Finnish (age range 22–27 years) adult twin pairs. Self-reported frequency of eating bread was obtained by food frequency questionnaires. Univariate models based on linear structural equations for twin data were used to estimate the relative magnitude of the additive genetic, shared environmental and individual environmental effects on bread eating frequency and choice of bread. The analysis of bread intake frequency demonstrated moderate heritability ranging from 37–40% in the Finnish cohort and 23–26% in the Danish cohort. The genetic influence on intake of white bread was moderate (24–31%), while the genetic influence on intake of rye bread was higher in men (41–45%) than in women (24–33%). Environmental influences shared by the twins were not significant. Consumption of bread as well as choice of bread is influenced by genetic predisposition. Environmental factors shared by the co-twins (e.g., childhood environment) seem to have no significant effects on bread consumption and preference in adulthood.

Novel technologies to reduce tillage in organic systems include a no-tillage roller/crimper for terminating cover crops prior to commercial crop planting. The objective of this experiment was to compare: (1) weed management and yield effects of organic tilled and no-tillage systems for corn (Zea mays L.), soybean [Glycine max (L.) Merr.] and irrigated tomato (Lycopersicon esculentum Mill.), using a roller/crimper and two cover crop combinations [hairy vetch/rye (Vicia villosa Roth/Secale cereale L.) and winter wheat/Austrian winter pea (Triticum vulgare L./Pisum sativum L. ssp. arvense (L.) Poir.)]; and (2) the economic performance of each system. Weed management ranged from fair to excellent in the organic no-tillage system for soybean and tomato crops, with the rye/hairy vetch mulch generally providing the most weed suppression. Corn suffered from low rainfall, competition from weeds and hairy vetch re-growth and, potentially, low soil nitrogen (N) from lack of supplemental fertilization and N immobilization during cover crop decomposition. No-tillage corn yields averaged 5618 and 634 kg ha−1 in 2006 and 2007, respectively, which was 42–92% lower than tilled corn. No-tillage soybeans in 2007 averaged 2793 kg ha−1 compared to 3170 kg ha−1 for tilled soybeans, although no-tillage yields were 48% of tilled yields in the dry year of 2006. Irrigated tomato yields averaged 40 t ha−1 in 2006 and 63 t ha−1 in 2007, with no statistical differences among tillage treatments. Economic analysis for the three crops revealed additional cover crop seed and management costs in the no-tillage system. Average organic corn returns to management were US$1028 and US$2466 ha−1 greater in the tilled system compared to the no-tillage system in 2006 and 2007, respectively, which resulted mainly from the dramatically lower no-tillage yields. No-tillage soybean returns to management were negative in 2006, averaging US$ −14 ha−1, compared to US$742 ha−1 for tilled soybeans. However, in 2007, no-tillage soybean returns averaged US$1096 ha−1. The 2007 no-tillage irrigated tomato returns to management averaged US$53,515 compared to US$55,515 in the tilled system. Overall, the organic no-tillage soybean and irrigated tomato system demonstrated some promise for reducing tillage in organic systems, but until economic benefits from soil carbon enhancement can be included for no-tillage systems, soil improvements probably cannot offset the economic losses in no-tillage systems. Irrigation could improve the performance of the no-tillage system in dry years, especially if grain crops are rotated with a high-value irrigated tomato crop.

It has been demonstrated that intact plasma alkylresorcinols (AR) and urinary AR metabolites could be used as biomarkers of whole-grain intake. Thereafter, we developed the method for the plasma AR metabolites, which is more convenient and requires less sample pretreatment than the analysis of intact plasma AR. The aim of the present study is to evaluate whether AR metabolites measured in plasma, in the same population, could also be considered as useful biomarkers of cereal fibre. Fifty-six women were recruited in a cross-sectional and observational study. Dietary intake (5-d record) and plasma AR metabolites (3,5-dihydroxybenzoic acid, DHBA; 3-(3,5-dihydroxyphenyl)-1-propanoic acid, DHPPA) were measured. The relationship between plasma AR metabolites and cereal fibre intake was examined using partial correlation and stepwise regression. Cereal fibre intake correlated significantly with plasma DHBA (r 0·411; P = 0·002) and DHPPA (r 0·463; P = 0·000) even after adjustment for BMI and age. Thus, plasma AR metabolites correlate with cereal fibre intake as noted with plasma intact AR and urinary AR metabolites. We observed that plasma DHPPA was the independent predictor of cereal fibre intake, explaining 18 % of the variance (adjusted r2 0·176; P = 0·002). In epidemiological screening, it might be easier to obtain and to collect plasma than urine samples. In addition, the plasma AR metabolites half-life seems longer than those of intact plasma AR, and their measurements are more convenient, and faster. Thus, sum of plasma AR metabolites and more specifically plasma DHPPA seems to be good and specific biomarkers of cereal fibre intake.

DNA synthesis following the addition of water to excised embryos of non-dormant rye and to embryos of non-dormant and dormant genetic lines of Avena fatua has been examined. All the samples exhibit an early unscheduled DNA synthesis, have similar responses to DNA polymerase inhibitors and a similar increase in DNA ligase function for the first 24 h of imbibition, irrespective of whether they germinate or remain dormant. A β-polymerase-mediated DNA repair activity is indicated immediately upon imbibition with the stable incorporation of 3H-methyl-thymidine into high molecular weight DNA. Following γ-irradiation of dry or imbibed embryos, inhibitor results suggest the appearance of an additional α- or δ-polymerase activity in the ensuing repair. Abscisic acid permits the early unirradiated repair synthesis, but like aphidicolin, it inhibits replicative DNA synthesis and partly inhibits the post-irradiation incorporation of thymidine. DNA synthesis takes place continuously throughout dormancy of imbibed embryos: precursor incorporation into DNA cannot be chased in the short term and occurs in the absence of an S-phase or endoreduplication of nuclear DNA. It is proposed that dormant imbibed embryos maintain the integrity of the genome by a continuous but slow replacement of DNA steered by a non-amplifying and modified form of replicative DNA synthesis and that abscisic acid may play a determining role in this process.

Development of transgenic triticale as a platform for novel bio-industrial products is predicated on an environmental biosafety assessment that quantifies the potential risks associated with its release. Pollen-mediated gene flow to related species and conventional triticale varieties is one pathway for transgene movement. A tier 1 quantification of triticale hybridization was conducted by emasculating and hand pollinating flowers under greenhouse conditions. Approximately 2000 manual pollinations were conducted for each cross and its reciprocal between two triticale genotypes: a modern triticale cultivar (AC Alta) and primary triticale (89TT108), and common wheat, durum wheat and rye. The frequency of outcrossing, hybrid seed appearance and weight, and F1 emergence and fertility were recorded. Outcrossing, F1 emergence and fertility rates were high from crosses between triticale genotypes. Outcrossing in inter-specific crosses was influenced by the species, and the genotype and gender of the triticale parent. In crosses to common and durum wheat where triticale was the male parent, outcrossing was ≥73.0% and ≥69.5%, respectively, but ≤23.9% and ≤3.0% when triticale was the female parent. Overall, outcrossing with rye was lower than with common and durum wheat. F1 hybrid emergence was greater when triticale was the female parent. With the exception of a single seed, all wheat-triticale F1 hybrid seeds were non-viable when triticale was the male parent in the cross. Only seven durum wheat-triticale F1 hybrids emerged from 163 seeds sown, and all were produced with triticale 89TT108 as female parent. With rye, 8 F1 hybrids emerged from 38 seeds sown, and all were produced from crosses to AC Alta; five with AC Alta as the female parent and three as the male. Interspecific F1 hybrids were self-sterile, with the exception of those produced in crosses between common wheat and triticale where triticale was the female parent. Tier 2 hybridization quantification will be conducted under field conditions.

Integration of crops and livestock has the potential to provide a multitude of benefits to soil and water conservation and nutrient cycling efficiency, while reducing economic risk and increasing profitability. We conducted a field study from May 2002 to October 2005 to determine crop and cattle responses to three management factors on a Typic Kanhapludult in Georgia, USA. Summer grain/winter cover [sorghum (Sorghum bicolor L. Moench) or corn (Zea mays L.)/rye (Secale cereale L.)] and winter grain/summer cover [wheat (Triticum aestivum L.)/pearl millet (Pennisetum glaucum L. R. Br.)] were managed with either conventional tillage (CT) or no tillage (NT) and with or without cattle grazing of cover crops. All crops were successfully established, irrespective of tillage and cover crop management. Although pearl millet was often lower in the plant stand with NT than with CT, plants compensated with greater biomass on an area basis. Across years, grain yield of sorghum (1.9 Mg ha−1 during three seasons) and corn (7.3 Mg ha−1 in one season) was 25% greater under NT than under CT when the cover crop was not grazed. Wheat grain yield (2.7 Mg ha−1 during three seasons) was unaffected by tillage and cover crop management. Unharvested stover production of summer grain crops was greater with NT than with CT (6.5 versus 4.1 Mg ha−1; P<0.001). Grazing rye rather than allowing it to accumulate as surface residue reduced summer grain yield 23% and reduced standing grain-crop dry matter 26% under NT, but had no effect under CT. In contrast, grazing pearl millet rather than allowing it to accumulate as surface residue increased wheat standing dry matter yield by 25±14% (mean±standard deviation among 3 years and two tillage systems). Ungrazed cover crop production was greater under NT than under CT for rye (7.0 versus 6.0 Mg ha−1; P=0.03) and pearl millet (10.2 versus 7.6 Mg ha−1; P=0.01). Calf daily gain was either greater or tended to be greater under NT than under CT on rye (2.27 versus 2.09 kg head−1 d−1; P=0.15) and pearl millet (2.05 versus 1.81 kg head−1 d−1; P=0.05). Total cattle gain per grazing season was either greater or tended to be greater with NT than with CT on rye (350 versus 204 kg ha−1; P=0.01) and pearl millet (324 versus 277 kg ha−1; P=0.15). Net return over variable costs was greater with grazing than without grazing of cover crops (US$302 versus −US$63 ha−1; P<0.001). Livestock grazing of cover crops had variable effects on subsequent crop production, but increased economic return and diversity overall. Therefore, an integrated crop–livestock production system with conservation tillage is recommended as a viable option for producers to diversify farming operations to avoid risk, improve ecological production of crops, and potentially avoid environmental damage from soil erosion and nutrient loss.

Integration of rye (Secale cereale L.) cover crops into the corn (Zea mays L.) soybean [(Glycine max (L.) Merr.] rotation of the upper Midwest USA can provide many agronomic and agroecological benefits. Integration is made difficult by short growing seasons, but may be facilitated by management of key agroecological interactions such as those between rye and soil microbiota. Rye growth was measured and colonization by arbuscular-mycorrhizal fungi (AMF) was determined in greenhouse experiments using soils from seven different management systems from a long-term cropping-systems experiment in southwest Minnesota. Microbial effects on rye growth were not evident before vernalization, but at final harvest (4 weeks after vernalization) soil microbial populations reduced rye shoot and root growth, relative to a pasteurized control inoculum. At final harvest, shoot biomass in 2-year rotations was 17% greater than 4-year rotations, indicating that microbial populations selected for by 4-year rotations may be more deleterious or pathogenic than those selected for by 2-year rotations. Growth of three rye cultivars was examined in all inocula; cultivars differed in their mean response to soil microbiota and their ability to host AMF. These findings suggest that management factors affect interactions between rye and soil microbiota resulting in altered rye growth.

Rye bran contains a high content not only of dietary fibre, but also of plant lignans and other bioactive compounds in the so-called dietary fibre complex. Blood concentrations of lignans such as enterolactone have been used as biomarkers of intake of lignan-rich plant food. At present, evidence from studies in human subjects does not warrant the conclusion that rye, whole grains or phyto-oestrogens protect against cancer. Some studies, however, have pointed in that direction, especially in relation to cancers of the upper digestive tract. A number of prospective epidemiological studies have clearly shown a protective effect of whole-grain cereals against myocardial infarctions. A corresponding protective effect against diabetes and ischaemic stroke (brain infarct) has also been demonstrated. It seems reasonable to assume that these protective effects are associated with one or more factors in the dietary fibre complex.

Manufacturing of healthy whole-grain foods demands knowledge of process-induced changes in macro-, micro- and non-nutrients. The high content of dietary fibre is a challenge in relation to good product texture and sensory quality. The stability and bioavailability of bioactive compounds have a marked influence on the health effects of cereal foods. It was confirmed that sterols, folates, tocopherols and tocotrienols, alkylresorcinols, lignans, phenolic acids and total phenolics are concentrated in the bran layers of the rye grain, and are only present at low levels in the flour endosperm. The levels of folate and easily-extractable phenolic compounds increase in germination and sourdough baking, but there are negligible changes in the levels of sterols, lignans and alk(en)ylresorcinols. The levels of tocopherols and tocotrienols are reduced during the sourdough fermentation. In conclusion, many of the bioactive compounds in whole-grain rye are stable during food processing, and their levels can even be increased with suitable processing.