Asian corn borer, Ostrinia furnacalis Guenée (Lepidoptera: Crambidae), is a major pest in corn production, and its management remains a significant challenge. Current control methods, which rely heavily on synthetic chemical pesticides, are environmentally detrimental and unsustainable, necessitating the development of eco-friendly alternatives. This study investigates the potential of the entomopathogenic nematode Steinernema carpocapsae as a biological control agent for O. furnacalis pupae, focusing on its infection efficacy and the factors influencing its performance. We conducted a series of laboratory experiments to evaluate the effects of distance, pupal developmental stage, soil depth, and light conditions on nematode attraction, pupal mortality and sublethal impacts on pupal longevity and oviposition. Results demonstrated that S. carpocapsae exhibited the highest attraction to pupae at a 3 cm distance, with infection declining significantly at greater distances. Younger pupae (<12 h old), were more attractive to nematodes than older pupae, and female pupae were preferred over males. Nematode infection was highest on the head and thorax of pupae, with a significant reduction in infection observed after 24 h. Infection caused 100% mortality in pupae within 2 cm soil depth, though efficacy was reduced under light conditions. Sublethal effects included a significant reduction in the longevity of infected adults and a decrease in the number of eggs laid by infected females compared to controls. These findings underscore the potential of S. carpocapsae as an effective biocontrol agent for sustainable pest management in corn production, offering a viable alternative to chemical pesticides.

The importance of host cues to three species of steinernematid nematodes (Rhabdita: Steinernematidae) with different foraging strategies was compared. We presented host materials to nematodes in series to test responses to combinations of host cues. If a fixed hierarchy of cues is followed during foraging, parasites should respond most strongly to cues offered in natural order. Steinernema carpocapsae, an ambush forager, aggregated at the source of volatile host cues only after attachment to host cuticle. They also parasitized hosts more efficiently after contact with cuticle. Steinernema glaseri, a cruise forager, was unaffected by exposure to combinations of host cues. Steinernema feltiae, a nematode with characteristics of both ambushing and cruising, was affected by cue hierarchies when either contact or volatile cues were presented first. Host-associated materials encountered out of the context may not qualify as host cues for the ambush forager, S. carpocapsae. Perhaps the order in which cues are encountered is more predictable for ambushers than for cruisers. Therefore an ambusher's response to host materials has a more fixed contextual framework.

Third-stage (dauer) juveniles of the entomopathogenic nematode Steinernema feltiae from 3 laboratory populations were tested for infectivity to larvae of the sciarid fly Lycoriellia solani. Bioassay experiments were done to assess the effects of nematode application density, exposure time and host density on the proportion of individuals that established infection. When dauer stages were presented to a host singly, establishment rates of 28, 26 and 18% were obtained for the 3 populations, but the presence of conspecifics consistently increased the rate of establishment to 42, 33 and 21% respectively, at 20 nematodes per host. A modified binomial model was developed to show that nematode establishment was not related to application density in a linear fashion, as initial infection facilitated secondary colonization. The data suggest that 3 subpopulations of nematodes may be distinguished by their infection behaviour: a first group of individuals with the behavioural propensity to initiate infection in unparasitized insects, a second that only invaded infected hosts, and a third group of non-invaders that failed to establish in L. solani.

Based on the ability of bacterial associates of entomopathogenic nematodes to produce antibiotic compounds on artificial media, it has been commonly accepted that Xenorhabdus sp. and Photorhabdus sp. inhibit a wide range of invading microorganisms in insects infected with Steinernema spp. or Heterorhabditis spp. Therefore, the question of whether antibiotic compounds produced by the primary form of bacterial symbionts associated mutualistically with S. carpocapsae and H. bacteriophora explain why insect carcasses do not putrefy but provide nutritional requirements for insect parasitic rhabditoid nematodes to complete their life-cycle was examined. Laboratory bioassays of anti-bacterial activity on nutrient agar and during parasitism in larvae of Galleria mellonella have confirmed earlier observations that in vitro colonies of the primary form of X. nematophilus and P. luminescens produced agar-diffusible antibiotic compounds of a broad spectrum of anti-bacterial activity; their role in parasitism seems doubtful, however. This hypothesis is supported by a low antibiotic potency of a limited spectrum of anti-bacterial activity throughout the life-cycle of the parasites, principally in Galleria infected with S. carpocapsae. Since the lack of putrefaction cannot be explained simply by antibiotic inhibition of contaminating bacterial microflora, other competition mechanisms must be operating in parasitized insects. I postulated that a rapid and massive colonization of the insect body by nematophilic bacteria creates unfavourable conditions for the growth and multiplication of bacterial (proteolytic) contaminators making the insect carcass decay-resistant. In the case of H. bacteriophora, low antibiotic activity at an early stage of parasitism could support the colonization by P. luminescens of the host.

The potential of using the entomopathogenic nematode Steinernema feltiae to control the sweetpotato whitefly Bemisia tabaci (Gennadius) has been established in previous laboratory studies. However, laboratory studies can overestimate the level of control achieved by biocontrol agents in the glasshouse. Glasshouse trials are therefore required to confirm laboratory results before full-scale commercial development is considered. Under both controlled laboratory and glasshouse conditions high mortality of second instar B. tabaci (>90% and >80%, respectively) was recorded after application of S. feltiae. The efficacy of the biocontrol agent at various application rates was also investigated, where halving the rate of S. feltiae application caused no significant reduction in B. tabaci mortality on tomato foliage. Steinernema feltiae has shown much potential for incorporation into integrated pest management strategies for the control of B. tabaci.