The family Streblidae is a significant grouping of dipteran insects within the superfamily Hippoboscoidea, which parasitizes the body surface of bats. With the global spread of bat-related pathogens in recent years, Streblidae has gained increasing attention due to its potential for pathogen transmission. A sample of Brachytarsina amboinensis was sequenced on the B. amboinensis were obtained, compared with available Streblidae mitogenomes, and the phylogeny of Hippoboscoidea was reconstructed. The results indicate that the mitochondrial genome of B. amboinensis exhibits a relatively high degree of conservation, with an identical gene count, arrangement, and orientation as the ancestral insect's genome. Base composition analysis revealed a strong bias towards A and T in the base composition. Selection pressure analysis indicated strong purifying selection acting on cox1. Pairwise genetic distance analysis showed that cox1 evolved at a relatively slow rate. Regarding phylogenetic relationships, the constructed phylogenetic trees using Bayesian inference and Maximum Likelihood methods supported the monophyly of the Hippoboscoidea, Glossinidae, Hippoboscidae, and Nycteribiidae clades, with high nodal support values. Our research confirmed the paraphyly of the families Streblidae. In the familial relations between Nycteribiidae and Streblidae, New World Streblidae share a closer kinship with Nycteribiidae. This contrasts with prior findings which indicated that Old World Streblidae share a closer kinship with Nycteribiidae. This study not only enhances the molecular database for bat flies but also provides a valuable reference for the identification and phylogenetic analysis of Streblidae.

Insects' development can be significantly impacted by various environmental factors, including temperature. Thus, this study aimed to investigate the effect of temperature on the predatory thrips, Scolothrips longicornis Priesner (Thysanoptera: Thripidae), which feeds on the strawberry spider mite, Tetranychus turkestani Ugarov and Nikolski (Trombidiformes: Tetranychidae). Under laboratory conditions (16:8 L:D, 75 ± 5% RH), the impact of various temperature regimens (15–37.5°C) on the development, population parameters, and mass production of S. longicornis was assessed. Additionally, an age-stage, two-sex life table of the species was constructed. The study revealed that S. longicornis' pre-adult developmental period decreased as temperature increased until 35°C, after which the developmental period increased. The longevity of both males and females displayed significant differences across the temperature range, with the longest lifespan observed at 15°C and the shortest at 37.5°C. At 26°C, the mean total fecundity of S. longicornis was significantly higher (53.52 eggs per female) than the fecundity observed at other temperature regimens. The intrinsic rate of increase (r) and finite rate of increase (λ) demonstrated the highest values at 35°C. While R0 increased as the temperature rose from 15 to 30°C, it rapidly decreased at 35 and 37.5°C. The findings of this study suggest that temperature plays a crucial role in enhancing the rate of development and reproduction of S. longicornis, and a temperature range of 26–30°C could be considered optimal for rearing and mass production of S. longicornis.

The generalist predatory mite, Neoseiulus cucumeris (Oudemans) (Acari: Phytoseiidae) is one of the most effective biocontrol agents to control the pests of many crops in indoor cultivations. In this study, the effects of the enriched diets of the second trophic level, i.e. the stored-product mite, Tyrophagus putrescentiae (Schrank) on the performance of N. cucumeris as the third trophic level was determined in a tritrophic system. In the first step, different pollens including almond, maize, date palm, castor bean, saffron, and cattail or different legume flours including pinto bean, lentil, black-eyed pea, chickpea, mung bean and broad bean as enrichment additives were added to the basic diet, i.e. a mixture of wheat bran and flour as a basic diet of T. putrescentiae. In the second step, to reveal the effects of the mentioned additives on the performance of N. cucumeris, the demographic parameters of the predator were determined when it was fed with the prey enriched with the additives. Our results indicated that N. cucumeris had higher performance by feeding on the prey reared on diets enriched by either pollens or legumes compared with the basic diet. Overall, there was no significant difference between pollen grains and some legume flours when the predatory mite was fed with them through its prey. Since legumes are more available and cost-effective food sources than pollens, they can be affordable supplementary diets for the mass rearing of N. cucumeris.

In December 2017 the Venetian Region (local Authority), financed the creation of the Operational Group (OG) ‘Serinnovation’, within the framework of the Rural Development Plan supported by the European Community. The OG aims at coordinating and spreading innovation in sericulture through mechanisation of processes and centralisation of some rearing steps, the use of waste as by-products and traceability to promote local productions. The project acts on perceived quality by increasing the added value, through production cost efficiency, and on the recovery of the waste material for further applications (circular economy). The final target was to develop a niche-process to obtain traceable ‘Made-in-Italy’ silk for the luxury market and non-textile applications. A first strategy to increase the efficiency of the process was to build an automatic leaf cutting machine to prepare the feed for the first three instars of the silkworm (Bombyx mori Linnaeus). This new machine – based on a patent – was validated through several tests and compared with the cutting system previously used. The study was completed by a bioassay of production and survival rate associated with the introduction of this innovation. The results showed that labour saving is in the order of 10% compared to a semi-manual process, the leaf quality is not affected, survival of larvae and silk production are not significantly different from the control. This methodology is proposed as a study case for other similar mechanisation processes in entomological production, as the impact of innovations on insect physiology should be carefully considered.

One of the most prominent problems related to biological invasions is the variation of local species composition, which often leads to ex novo interspecific interactions. Here, we explored and analysed the native species composition of gall inducers and their associated parasitoids and inquilines in Spanish areas invaded by Dryocosmus kuriphilus Yasumatsu 1951 (Hymenoptera: Cynipidae), an invasive pest of chestnut trees. After a quantitative description of these species' assemblages, we analysed through bipartite networks the level of the trophic specialisation of parasitoids and inquilines when considering either the host taxonomic identity, the host plant species or the host gall morphological type. We sampled galls of D. kuriphilus and native species of Cynipidae in different Spanish areas, including those where the exotic parasitoid Torymus sinensis Kamijo 1982 (Hymenoptera: Torymidae) had been released for D. kuriphilus biological control. The results indicate that the native parasitoids recruited by D. kuriphilus come almost exclusively from native communities on Quercus galls, except for one species from Rosa. Galls of D. kuriphilus had the second most diverse species composition; despite this species assemblage arose ex novo in less than a decade. The bipartite networks resulted more specialised when considering host plant taxa than when gall types and the host taxa were accounted. In such trophic webs, there were few parasitoid/inquiline specialist and many generalist species, which agrees with the rapid recruitment by D. kuriphilus. Higher parasitoid species richness in D. kuriphilus galls is likely due to their being a largely unexploited available resource for the native natural enemies of cynipid wasps.

Cadra cautella is a serious insect pest of stored figs and dates. The irradiation sensitivity of different development stages of C. cautella and large-scale testing of the proposed irradiation quarantine doses (50–500 Gy), were investigated. The impact of a PI dose of 400 Gy on the physiochemical and microbiological quality of dry dates (Bartamoda cv.) stored at room temperature was also investigated. An irradiation dose of 100 Gy prevented egg hatching in the F1 generation when 1–3 days old eggs were irradiated. Irradiation doses of 200 and 300 Gy prevented adult emergence when 2nd and 4th instar larvae were irradiated. When the pupae stage was irradiated, an irradiation dose of 400 Gy prevented the hatchability of F1 generation, indicating that this stage was the most radio-tolerant. The results of large-scale testing of the proposed phytosanitary irradiation dose (400 Gy) applied to 18, 0000 pupae resulted in no reproduction (zero hatching of F1 generation). There were no significant differences in the physiochemical properties of stored dates during the storage period at room temperature. Stable ESR signal intensity was recorded for 6 months in all parts of the irradiated fruits, and the intensity was highest in the kernel. The PI dose of 400 Gy also slightly reduced all microorganisms' counts. In conclusion, the dose level of 400 Gy stopped the reproduction potential of C. cautella. and they maintained the quality characteristics of dry date Bartamoda fruits during storage at room temperature for 6 months in tightly closed packages.

Triatomines (Hemiptera: Heteroptera: Reduviidae) are hematophagous insects, well-known for their vectorial role in transmitting Trypanosoma cruzi Chagas (Kinetoplastida: Trypanosomatidae) parasites, the etiological agent of Chagas disease. Trapping these insects would limit human-triatomine interaction and, thus, control the disease. In this context, there is a critical need for effective lures to control triatomines. Through double-choice bioassays, we investigated the preference of Triatoma infestans Klug, T. pallidipennis Stal, and Rhodnius prolixus Stal triatomines for: (a) volatiles from fermented products (various fermentation types and substrates) and (b) commercial insect lures. Furthermore, we identified the chemical composition of these volatiles through headspace collection using Solid Phase Micro Extraction coupled with Gas Chromatograph-Mass Spectrometer (HS-SPME-GC-MS). Volatiles from lactic fermentation and certain fermented fruits, along with commercial lures, attracted triatomines, while other products exhibited possible repellent or dislodging properties. These findings hold promise for the control of triatomines and, ultimately, Chagas disease.

Phytoplasmas are phloem-limited bacteria that are primarily transmitted by hemipteran insects and are emerging threats to Camptotheca acuminata Decne plants due to their associations with a witches’ broom disease. Despite numerous studies, there has been no report on insect transmission of phytoplasma among C. acuminata. Here, transmission characteristics of the leafhopper, Empoasca paraparvipenis Zhang and Liu, 2008 and the phytoplasma in plant leaves through PCR quantification are described. The interaction between C. acuminata-phytoplasma and insect vectors was examined by analysing the impact on the life characteristics and progeny population in a temperature-dependent manner. Phytoplasma-infected C. acuminata plant exhibited symptoms including shorter internodes, weak and clustered branches, shrunken and yellowed leaves, and red leaf margins. The acquisition and transmission time of bacterial-infected third-instar nymphs of insect vectors were 10 (11.11%) and 30 min (33.33%), respectively. A single insect vector can infect a plant after 72 h of feeding, and the incidence rate of disease increases with the number of insects following 11–100% from single to 20 insects. The development time of the infected insect vectors (1–3 instars) was significantly shorter than that of the healthy insects, and the development duration of instar individuals was longer. In progeny populations, the higher the phytoplasma concentration (88–0% for 1–5 instars nymph, female and male adults), the shorter the development time and the longer the adult lifetime (both male and female). These findings provided research evidence of phytoplasma transmission by insect vectors; however, further investigation of the mechanisms for prevention and management of phytoplasma diseases is needed.

There is a need for comprehensive research on the species structure and the population dynamics of the most common aphidophagous species. A critical factor of the effectiveness of aphid biocontrol is the ratio of beneficial polyphagous (generalist) to oligo- or monophagous (specialist) species within the various trophic groups. Aphids' population density and environmental conditions influence the development and potential feeding of useful insects. The present study aimed to determine the community structure, relationships and diversity between aphids and their aphidophagous species in alfalfa fields using the following methods: sweeping with an entomological net, the quadratic method, coloured sticky board method, route survey method and visual observations. Research on the structure of the aphid–aphidophagous community revealed that aphidophagous species belong to three groups: (1) polyphagous predatory bugs from the families Anthocoridae and Nabidae, (2) oligophagous and polyphagous predators from the families Coccinellidae, Syrphidae and Chrysopidae; and (3) monophagous and oligophagous parasitoids, primarily from the families Aphidiidae and Ichneumonidae. From mid-May to June, there was a sufficiently large potential for aphidophagous species (Coccinellidae, Syrphidae, Chrysopidae, Anthocoridae and Nabidae) to control aphids, while in September, predatory ladybirds from the Coccinellidae family were the main biological control agents. Coccinellidae (Coleoptera) exhibited the highest values of diversity, dominance and richness indices among insect groups in the aphid–aphidophagous community. The existence of diverse aphidophagous species in alfalfa fields suggests that these predators can complement each other, leading to effective biological control of aphids. The synergy among different predator species holds promise for enhancing the overall efficacy of integrated pest management strategies.

Negative relationships between the parental age and offspring life history traits have been widely observed across diverse animal taxa. However, there is a lack of studies examining the influence of parental age on offspring performance using mites, particularly phytoseiid predators as subjects. This study explored the influence of maternal age on offspring life history traits in Amblyseius herbicolus (Chant) (Acari: Mesostigmata), a phytoseiid predatory mite reproducing through thelytokous parthenogenesis. We hypothesised that increased maternal age negatively impacts offspring traits, including developmental duration, body size, fecundity and lifespan. Amblyseius herbicolus was reared under controlled laboratory conditions, and the life history traits of offspring from mothers of varying ages were analysed using linear mixed-effect models. Our results showed that the increase in maternal age significantly reduced individual egg volume, but did not significantly affect offspring developmental duration, body size, fecundity or lifespan. These findings indicate that while older A. herbicolus females produced smaller eggs, the subsequent performance (i.e. body size, fecundity and lifespan) of offspring remained largely unaffected, suggesting possible compensatory mechanisms in the offspring or alternative maternal provisioning strategies. The results of this study offer useful insights into the reproductive strategies of phytoseiid predators and asexually reproducing species, enhancing our understanding of how maternal age affects offspring fitness. Further studies can examine how offspring of A. herbicolus from mothers of different ages perform under adverse environmental conditions.

The fruit fly Ceratitis capitata is a successful generalist due to the trade-off between different variables in its life history traits. The present study aimed to assess some life history traits of C. capitata recovered from peach, plum and fig, three key host species, under the climatic conditions of an irrigation oasis in the arid province of San Juan, Argentina. Pupal abundance, sex ratio and morphometric parameters such as pupal and adult weight, pupal volume, wingspan and head-caudal length were influenced by the host in which the larvae were reared. Sexual maturity, measured as the peak of calling (pheromone emission), was homogeneous on the sixth and seventh days after emergence, but males recovered from fig showed early calling activity on the third day after emergence. The plasticity of C. capitata to adapt its life history traits to specific nutrient variations within the larval environment allows it to colonise and establish populations where key hosts, as those studied here, are available. The knowledge of the interactions between host fruit trees and C. capitata populations provides helpful information for planning the management of local orchards and urban fruit plantations within irrigation oases, taking into consideration the concept of key hosts as peach and fig.

Invasive species threaten biodiversity on a global scale. The success of invasions depends on the species' adaptation to the different environmental conditions of new territories. Studies show that invasive insects present evolutionary changes in wing morphology in areas they are introduced to in response to abiotic conditions. In the last decade, the Asian Drosophila nasuta fly invaded and spread widely throughout Brazil. This insect has preferences for conserved environments and is related to the likely reduction in the abundance of native drosophilids in the Atlantic Forest. Ecological niche modelling analyses showed that rainfall and temperature are the main factors which delimit the geographic distribution of this species. Herein, we verified the existence of significant differences in the wing sizes of D. nasuta in Brazil and evaluated the influence of abiotic factors (rainfall and temperature) on the observed patterns. We conducted 11 measurements on the right-side wings of 240 D. nasuta males collected in the Amazon Forest, Caatinga, Cerrado and Atlantic Forest. Statistical analyses revealed the existence of two groups: one with larger wings, which brought together samples from locations with the lowest temperatures; and one with smaller wings, which corresponded to places with a hotter climate. One explanation for this result is the fact that large wings favour greater heat capture by flies in colder climates, increasing their survival chances in these environments. These rapid evolutionary changes in D. nasuta in this first decade of invasion in Brazil reveal the enormous adaptive potential of this species in this megadiverse country.

Paranosema locustae is an environmentally friendly parasitic predator with promising applications in locust control. In this study, transcriptome sequencing was conducted on gonadal tissues of Locusta migratoria males and females infected and uninfected with P. locustae at different developmental stages. A total of 18,635 differentially expressed genes (DEGs) were identified in female ovary tissue transcriptomes, with the highest number of DEGs observed at 1 day post-eclosion (7141). In male testis tissue transcriptomes, a total of 32,954 DEGs were identified, with the highest number observed at 9 days post-eclosion (11,245). Venn analysis revealed 25 common DEGs among female groups and 205 common DEGs among male groups. Gene ontology and Kyoto Encyclopaedia of Genes and Genome analyses indicated that DEGs were mainly enriched in basic metabolism such as amino acid metabolism, carbohydrate metabolism, lipid metabolism, and immune response processes. Protein–protein interaction analysis results indicated that L. migratoria regulates the expression of immune- and reproductive-related genes to meet the body's demands in different developmental stages after P. locustae infection. Immune- and reproductive-related genes in L. migratoria gonadal tissue were screened based on database annotation information and relevant literature. Genes such as Tsf, Hex1, Apolp-III, Serpin, Defense, Hsp70, Hsp90, JHBP, JHE, JHEH1, JHAMT, and VgR play important roles in the balance between immune response and reproduction in gonadal tissues. For transcriptome validation, Tsf, Hex1, and ApoLp-III were selected and verified by quantitative real-time polymerase chain reaction (qRT-PCR). Correlation analysis revealed that the qRT-PCR expression patterns were consistent with the RNA-Seq results. These findings contribute to further understanding the interaction mechanisms between locusts and P. locustae.

Ectropis grisescens Warren and Ectropis obliqua (Prout) are two morphologically similar sibling species with overlapping ranges. In this study, manipulative laboratory experiments were conducted to examine the possibility of reproductive interference in sympatric populations of E. grisescens and E. obliqua and the potential consequences of the mating interaction. Our results showed that the presence of males or females of different species could incur mating interference and significant reduction of F1 offspring. The reduction was not significant relevant to the initial relative abundance of E. grisescens and E. obliqua. Detailed observations of mating opportunity showed that female mating frequencies of both species were not significantly affected by the absolute species density, but the mating success of E. obliqua females with conspecific males depended on species ratio. In addition, adding males to the other species resulted in lower number of offspring suggesting that the males’ behaviour might be linked with mating interference. Males of both E. grisescens and E. obliqua could interfere the intraspecific mating of the other species, but the impact of the mating interference differed. These combined data indicated that asymmetric reproductive interference existed in E. grisescens and E. obliqua under laboratory conditions, and the offspring of the mixed species were significantly reduced. The long term outcome of this effect is yet to be determined since additional reproductive factors such as oviposition rate and progeny survival to adulthood may reduce the probability of demographic displacement of one species by the other in overlapping niches.