Parrots (Psittaciformes) are widely kept in captivity, yet their welfare is under-researched in comparison to other captive species. This study aimed to determine key welfare issues affecting parrots through a modified Delphi approach. Twenty-eight welfare issues were first compiled via a preliminary literature review. Parrot welfare experts and sector professionals (n = 26) were then recruited to participate in an online survey to rank the identified welfare issues on a six-point scale according to severity, duration and prevalence of each issue. Participants could provide commentary on their ranking and propose additional welfare issues of concern. Items with a mean score of 4 or above progressed to a second survey, where participants (n = 14) indicated whether they agreed or disagreed with the current ranking of the welfare issue. Finally, two online workshops were held, where participants (n = 7) discussed the rankings from the second survey and sought to establish a consensus on the top ten welfare issues in each category and overall. Six of the seven final participants agreed with the final rankings, achieving a consensus rate of 86%. The top welfare issues overall were lack of owner knowledge and support; social isolation; housing; environmental opportunity to express behaviours; nutrition; development of normal behaviour; lack of a ‘life plan’ for birds; abnormal behaviours; lack of parrot-specific veterinary training; and insufficient application and enforcement of legislation. It is hoped that identification and recognition of these priority areas will be useful in directing future efforts in research, owner and veterinary education, and policy initiatives to improve parrot welfare.

Finding effective ways to monitor laying hen welfare is challenging as UK flock sizes can reach 16,000 birds. Eggs provide potential for welfare monitoring, as they are a daily output with previous evidence of links to stress. We explored the associations between stressors and eggs using two complementary studies. In Study 1, hens experienced social or heat stressors and eggs were scored daily for defects in shell characteristics. All eggs were scored on a three-point scale: 1 (no defect); 2 (minor defects); or 3 (unsuitable for whole egg sale in the UK). Texture defects were higher after stress treatments and were explored further as a promising proxy measure of welfare. In Study 2, eggshell texture from five commercial flocks was scored before versus at the onset of an avian influenza-enforced indoor housing, and scores were correlated with industry data for egg quality. Eggs were more likely to have texture defects after the enforced indoor housing, and manually scored texture correlated significantly with shell strength and shell colour during automated grading. Shell strength was weaker immediately after the enforced indoor housing and eggs were darker. We suggest that eggshell texture could be a useful addition to assessing changes or stresses in a hen’s environment for both research and commercial purposes, but further validation is needed to understand the generalisability of these results to other stressors. Additionally, data already collected in factories, such as shell strength and colour, may provide information on stress and could be valorised for understanding hen welfare.

Behavioural complexity is likely to reflect how animals cope with their environment. A large behavioural repertoire and the ability to flexibly apply these behaviours provide an individual with a greater likelihood of adapting to its (captive) environment. Here, we developed a procedure to aggregate different behavioural measures into a condensed measure of behavioural complexity based on 14 features, which were previously proposed (e.g. number of behaviours, Shannon diversity index) as well as some new features of behavioural complexity (e.g. variances of within and between transition durations). To test the measure, artificial behavioural sequences with potentially varying complexity were created using an individual-based modelling approach. With a Principal Component Analysis, the features extracted from these sequences could be reduced to two components (‘general complexity’ and ‘state variability’) explaining 59.64 and 27.68% of the total variance, respectively. The effect of the aspects of the artificial behavioural sequences on ‘general complexity’ and ‘transitions variability’ were analysed using linear mixed-effects models. The number of behavioural categories and the proportion of short behavioural states had the largest effect on the components. Both components were increasing with a greater number of behavioural categories, whereas the proportion of short behavioural states the opposite effect on the components. We also tested the approach on real data-sets. The principle components were not identical to the ones from the simulation. Yet, we found consistencies and similarities in the loadings. The approach for an aggregated measure of behavioural complexity developed here could form the basis of an individual-based animal welfare indicator for intensively kept animals.

Many companion kittens entering shelters are fostered by volunteer community members during the sensitive period for socialisation (~2 to 9 weeks of age) when early experiences are critical to behavioural development. Using a mixed-method survey, we explored current fostering practices relevant to kitten behavioural development and welfare. Foster caretaker participants (n = 487) described their fostering practices and reported providing kittens with a majority of recommended socialisation experiences, such as handling and exposure to various toys and exploratory items. In open-ended text responses, foster caretakers described how they adapted socialisation practices for fearful kittens and the supports and challenges they perceived to impact their ability to properly socialise kittens. Some non-recommended techniques (e.g. flooding) were reported for socialising fearful kittens, with a decreased odds of reporting non-recommended techniques for participants with a higher level of agreeableness personality trait and an increased odds of reporting if fostering practices had been impacted by the COVID-19 pandemic. Foster caretakers reported feeling supported through shelter-supplied resources, personal knowledge, external support, and having access to socialisation opportunities; however, faced personal (e.g. time constraints), shelter-specific (e.g. lack of shelter support), and kitten-specific challenges (e.g. kitten illness). This study highlights the perspectives of foster caretakers as related to optimal socialisation, behavioural development, and welfare. To identify opportunities for improvement it is important to investigate the socialisation guidelines provided to foster caretakers, with the ultimate goal of enhancing kitten behavioural development for improved welfare, long-term adoption, and caretaker satisfaction.

Horses employ a range of subtle to overt behaviours to communicate their current affective state. Humans who are more cognisant of their own bodily sensations may be more attuned to recognising affective states in horses (Equus caballus) thereby promoting positive human-horse interactions. This study investigated human ability to categorise human-horse interactions depicted in media relative to equine behaviour experts and compared participant scores to their level of interoception. Using an online survey, participants (n = 534) categorised 31 photographs and videos as (overt) positive, likely (subtle) positive, neutral, likely (subtle) negative or (overt) negative human-horse interactions from the horse’s point of view and completed the Multidimensional Assessment of Interoceptive Awareness questionnaire (MAIA-2) to assess their level of interoception. Demographic information was also collected (age, gender, education, level of experience with horses, location). Participants differed from expert categorisations of horse affective states across all categories, exactly matching experts only 52.5% of the time and approximately matching experts for positive and negative valence 78.5% of the time. The MAIA-2 did not predict participant ability to accurately categorise human-horse interactions. Women outperformed men in categorising overt positive, overt negative and subtle negative human-horse interactions. Increased levels of education and greater experience with horses were associated with improved categorisation of certain human-horse interactions. More training or awareness is needed to recognise behavioural indicators of horse affect to guide appropriate human-horse activities that impact horse welfare.

The activity level and specific behaviours exhibited by captive animals are crucial indicators of welfare. Stereotypies, or repetitive behaviours that have no apparent function or goal, are performed by animals experiencing poor conditions in their environment and indicate welfare concerns. Changes in the housing environment in particular may have critical influences on behaviour and welfare. Here, we measured behavioural changes in a captive pinyon jay (Gymnorhinus cyanocephalus) population (n = 10) associated with a shift from single to pair housing. Using automated video processing, we show that pair housing greatly reduced overall activity levels in these birds. The stark reduction in activity was surprising, as we expected that social housing would increase interactions between birds, thus increasing activity levels. Upon further analysis, however, we found that stereotypic behaviours, such as beak scraping, jumping, pecking, and route tracing decreased after pair housing, whereas the positive welfare behaviours of perching and preening increased. Our results indicate that pair housing may reduce overall activity in pinyon jays; however, this reduction is primarily in stereotypic behaviours.

The emotional valence hypothesis suggests an increased left-brain hemisphere activation during positive situations and vice versa during negative situations. Since facial thermal asymmetries may reflect lateralised brain activity, we investigated this in dairy calves subjected to hot-iron disbudding (Disbudded; n = 12) as a model of negative affective states. As affective responses can vary due to previous experiences, we examined whether calves that had (ExpObs; n = 12) and had not (InexObs; n = 12) experienced disbudding differed in their thermal response to a conspecific being disbudded, and whether calf response to the researcher (approaching, moving away, not moving) was associated with thermal asymmetries. We made thermographic recordings of each calf on three days: Day before disbudding (D1); Disbudding day (D2); and Day after disbudding (D3), and at two different times: Disbudding time/1400(T1) and Afternoon/1700(T2). Data were analysed using multilevel models. Calves had warmer left ears on D2 compared to higher temperatures on the right ear on D1, suggesting higher right-hemisphere activity on D2. ExpObs calves had higher left-eye temperatures when observing a conspecific being disbudded (D2×T1) than InexObs calves that had warmer right eyes, but this reversed on the following day (D3×T1). Calves avoiding the researcher had warmer left eyes whereas those approaching him had warmer right eyes, suggesting greater activation of the right hemisphere in the former. This study provides initial evidence of temperature asymmetries when observing or experiencing a negative event. Further work is required to confirm and build upon these early findings. The study highlights the potential for future development of methods using infrared thermography as a proxy measure of affective valence.

Pain, a multifaceted condition associated with actual or potential tissue damage, transcends nociception and is characterised as a subjective, sensory, and emotional experience. Extensive literature describing the adverse effects of untreated post-surgical pain emphasises the necessity of a comprehensive pain management protocol, incorporating both pharmacological and non-pharmacological strategies to ensure successful patient outcomes. The present study aimed to determine whether a positive dog-owner interaction influences post-operative pain perception and stress (POPPS), as well as behavioural inactive rate variability in bitches that underwent elective surgery. Randomly selected bitches (n = 18) underwent ovariohysterectomy. Eight bitches experienced a 45-min visit post-surgery (VPS) characterised by positive dog-owner interaction, while the remaining ten did not (NVPS). Utilising the validated Short Form of the Glasgow Composite Measure Pain Scale (CMPS-SF) to assess acute pain in dogs via stress-related behaviours, a significant decrease in POPPS was evident in the VPS group after the 45-min dog-owner interaction at T3 (1 h after post-sedation recovery), in contrast to the NVPS group. CMPS-SF-associated descriptive items ‘Nervous/Anxious/Fearful’ and ‘Happy Content or Happy and Bouncy’ decreased and increased, respectively, with dog-owner positive interaction in the VPS group. The inactivity rate was significantly lower in VPS bitches after the post-surgery 45-min dog-owner interaction than in NVPS bitches. This preliminary study suggests that the owner’s presence reduces POPPS and may improve the dogs’ welfare while undergoing routine surgeries.

Post-racing thoroughbreds (TBs) are increasingly being considered for Equine Assisted Services (EAS), but their use has not yet been widely characterised. This study aimed to generate detailed data on TBs and other breeds (OBs) of horses in EAS via an online survey. The survey was completed by 129 EAS practitioners from 15 countries and reported detailed data on 427 EAS equids, including 57 TBs. Most of the EAS horses were housed collectively, had access to free exercise over 12 h per day and had unrestricted access to forage. The most commonly recorded selection criteria for EAS horses consisted of: demonstrating a good personality; enjoying the work; absence of propensity to kick/bite. Detailed data gathered on individual horses showed that in comparison to OBs, TBs were younger, more likely to be a gelding, less likely to be used in ridden programmes, and tended to present more behavioural issues than OBs. The majority of the participants agreed that TBs have specific assets of particular interest to EAS programmes, such as sensitivity, body/movement characteristics or responsiveness/flight response. Finally, a large majority of participants reported that they believe TBs to be suitable for EAS programmes and some would consider working with them. These results showed TBs to already be in use in various EAS programmes and more could be incorporated in the future. In terms of animal welfare and beneficiaries’ safety, a selection process could therefore be designed and implemented to choose the most adapted horses for each EAS centre, according to living conditions and EAS activities practised (ridden or not ridden).

In-farm livestock production vaccinations are commonly delivered intramuscularly using needles. While there are alternative strategies these have been subject to little attention and limited commercialisation. One such alternative is needle-free vaccines and studies have focused on the immune response few have addressed the welfare implications. This study aims to compare the impact of intradermal needle-free vaccination and intramuscular injection in terms of the welfare of the piglets. A total of 179 piglets were divided into two treatments: intradermal needle-free delivery and intramuscular delivery of a vaccine. Measures of health and welfare included, vocalisations, behavioural observations, papule formation, and weight. Piglets vaccinated via the needle-free intradermal route vocalised less and displayed no significant behavioural differences but showed increased weight compared to piglets vaccinated intramuscularly. The use of a needle-free device to deliver a vaccine through an intradermal route revealed no adverse effects on piglet welfare and supports the use of alternative strategies to vaccinate livestock.

Both the nature of the human-animal relationship (HAR) and housing conditions significantly impact the welfare of farmed animals. To evaluate the influence of HAR on the behaviour, emotions and stress of rabbits (Oryctolagus cuniculus) in two distinct outdoor living environments, we allocated 144 young rabbits to four groups (CPX-H, CPX-N, SPL-H, SPL-N) differing in the living environments (CPX for complex, and SPL for simple). The treatment by human (H) involved daily provision of additional food resources and stroking (thoughtful farmer). It commenced at 49 days of age and lasted for 16 days. N groups did not receive the treatment (distant farmer). The rabbits were observed between 48 and 73 days of age. Their behavioural responses to human presence were evaluated at 48 and 68 days using Qualitative Behaviour Assessment (QBA) and scan sampling. A set of tests was conducted at 68 days of age to assess their reactions to a novel object and human presence. Stress levels were measured by analysing corticosterone concentrations in their hair. Rabbits in the SPL environment spent significantly more time near the novel object than those in the CPX environment (24.7 vs 17.2%). Additionally, rabbits in the H treatment group spent more time near the human than those in the N treatment group (28.2 vs 17.1%) and accepted more strokes (90.2 vs 45.9%). Following the HAR treatment, rabbits in the H group were significantly more likely to be described as ‘Affectionate/Interested’ than those in the N treatment. Rabbits in the N treatment were described as ‘Indifferent’ significantly more in the SPL environment. However, there were no significant differences in hair corticosterone concentrations between the groups. These findings indicate that rabbits’ responses are influenced by both their living environment and the quality of their relationship with humans. Encouraging positive interactions with animals may enhance their welfare and facilitate daily care from farmers.

Signs of distress in dogs during veterinary visits are often normalised rather than viewed as welfare concerns. Interventions designed to reduce fear during veterinary visits were evaluated to see if they affected dogs’ behaviours compared to dogs without interventions. Twenty-eight dogs were examined at four visits across eight weeks. Dogs were randomised into intervention (distress reduction/adaptive care) and control groups (standard care) and evaluated via the Working Dog Questionnaire – Pet Dog Version (WDQ-Pet). At visit 1 (baseline) all dogs received the control protocol. Homework was assigned following visit 1 to practice collaborative examination (intervention) or to pet the dog (control) for the same allotted time. At each visit, behaviours were scored (clinical stress score) via video and in-person observations when dogs entered the hospital, stepped onto a scale to be weighed, entered the exam room, at the beginning and end of examination, and after venipuncture. There were no differences between groups at visit 1, or across visits entering the hospital or exam room. At visit 4, intervention scores either decreased or remained low when weighed, and at the beginning and end of the physical exam. Control scores were significantly higher than the intervention scores during these periods. Reduced clinical stress scores indicate intervention dogs had improved care experience compared to the control. The study results highlight the value of applying simple and adaptable interventions, ultimately leading to improved animal care and welfare.

Working equids support the livelihoods of millions of low-income households worldwide and face several welfare challenges. Although equipment-related wounds are common, little is known about specific risk factors. This cross-sectional study surveyed equids used for cart-work in three Ethiopian towns. Number, size, severity and location of wounds were recorded for each animal, as well as work equipment characteristics and indicators of equipment fit and assembly. Questionnaires were conducted with each cart-driver focusing on equipment practices and attitudes. Logistic regression models were generated to investigate associations between equipment-related wounds (outcome) and equipment, work, driver and animal-related factors (predictors). In total, 369 equids and cart-drivers were surveyed. The prevalence of equipment-related wounds was 72.6% (268/369) with girth wounds being the predominant wound type in horses (50%; 122/244) while donkeys had predominantly shaft-related wounds (59%; 72/122). Donkeys were two times more likely to have equipment-related wounds than horses. The presence of equipment-related wounds was associated with factors such as previously having wounds, inadequate collar positioning and using purchased equipment compared to partly home-made equipment. Characteristics of specific equipment components were also associated with certain wound types, such as narrow saddle pressure points and saddle wounds. Equipment-related wounds are highly prevalent in working equids, representing a serious welfare concern. Factors relating to equipment design, fit and assembly were associated with the presence of wounds. Importantly, species differences require tailoring of preventive approaches amongst working equids. A better understanding of work equipment should therefore be promoted as part of wound prevention and animal welfare strategies.

One of the major challenges in lameness management is prompt detection, especially before visible gait disturbance. This scoping review describes the potential biomarkers for lameness in dairy cows reported in the literature, their relevance in lameness diagnosis, identifying cows at risk of developing claw lesions and monitoring recovery after treatment. Using specific keywords, a comprehensive literature search was performed in three databases: PubMed, Google Scholar and ScienceDirect to retrieve relevant articles published between 2010 and 2022. A total of 31 articles fulfilling the inclusion criteria were analysed. The categories of potential markers for lameness reported in the literature included acute phase proteins (APPs), nociceptive neuropeptides, stress hormones, proteomes, inflammatory cytokines and metabolites in serum, urine and milk. Cortisol, APPs (serum amyloid A and haptoglobin) and serum, urinary and milk metabolites were the most studied biomarkers for lameness in dairy cows. While APPs, nociceptive neuropeptides and blood cortisol analyses assisted in elucidating the pain and stress experienced by lame cows during diagnosis and after treatment, evidence-based data are lacking to support their use in identifying susceptible animals. Meanwhile, metabolomic techniques revealed promising results in assessing metabolic alterations occurring before, during and after lameness onset. Several metabolites in serum, urinary and milk were reported that could be used to identify susceptible cows even before the onset of clinical signs. Nevertheless, further research is required employing metabolomic techniques to advance our knowledge of claw horn lesions and the discovery of novel biomarkers for identifying susceptible cows. The applicability of these biomarkers is challenging, particularly in the field, as they often require invasive procedures.

Quality of life is dependent upon the extent to which behavioural needs are met, and the balance between pleasant and unpleasant lifetime experiences. In Part II of this systematic review, articles (n = 109) relating to horse-human interactions were reviewed to identify behavioural evidence of their positive or negative impact on the horse. The number of articles (n = 22) relating to the recognition of pain in horses, indicated the importance of identifying health issues, which are also likely to increase the aversiveness of interactions. These and articles relating to emotional reactivity testing in horses (n = 19), the behaviour of horses during handling and management procedures (n = 17), behaviour of the horse when ridden (n = 17), non-procedural horse-human interactions (n = 13), horse behaviour during transportation (n = 12) and behaviour during training other than when ridden (n = 9) were reviewed. During most interactions, horse behaviour is controlled and/or restricted by the human, masking negative or positive signs, and may be confounded by factors including fear and individual differences. In situations involving freedom of movement, positive experiences of horses with humans were associated with approach behaviour, negative ones with avoidance, but training could affect both. Undoubtedly, change is needed to reduce the extent to which interactions with humans are unpleasant for the horse. Only when the needs of the horse are fulfilled and interactions with humans are predominantly pleasurable will their quality of life improve.

The welfare of horses involved in sport and leisure activities has come under increasing scrutiny, both from within the equine sector and from the public. A systematic review of scientific evidence was conducted to derive observable, evidence-based behavioural measures of positive or negative affective state and factors relating to quality of life in the domestic horse (Equus caballus). Two separate searches (including the terms ‘emotion/affect’, or ‘stress’) were conducted, with 179 articles being retained. In Part I of this two-part review (companion paper published simultaneously), articles relating to the extent to which the species-specific needs of the horse are fulfilled in the home environment (n = 21), in relation to social grouping (n = 20), and during weaning (n = 14) were reviewed. Experimental tests of affective state in relation to housing and management (n = 8), and studies relating to stereotypical behaviour (n = 7) were also included. Opportunities for behavioural expression were dependent upon the provision of social and physical resources. Withdrawn or agitated behaviours in situ, avoidance behaviour during handling and agonistic intra-species interactions were indicative of negative affective state. Affiliative intra-specific social interactions, and forage ingestion were considered positive signs. For horses to live a good life, their need for space, companionship, and forage must be prioritised from birth and throughout their lives.

According to the Bayesian brain hypothesis, the brain can be viewed as a predictive machine, such that predictions (or expectations) affect how sensory inputs are integrated. This means that in many cases, affective responses may depend more on the subject’s perception of the experience (driven by expectations built on past experiences) rather than on the situation itself. Little research to date has applied this concept to affective states in animals. The aim of this paper is to explore how the Bayesian brain hypothesis can be used to understand the affective experiences of animals and to develop a basis for novel predictions regarding animal welfare. Drawing from the literature illustrating how predictive processes are important to human well-being, and are often impaired in affective disorders, we explore whether the Bayesian brain theories may help understanding animals’ affective responses and whether deficits in predictive processes may lead to previously unconsidered welfare consequences. We conclude that considering animals as predictive entities can improve our understanding of their affective responses, with implications for basic research and for how to provide animals a better life.

The use of animals as scientific models is argued to be crucial for producing new scientific and medical knowledge and clinical treatments. However, animal research continues to raise socio-ethical concerns. In recent years, there has been a push for openness amongst the life science community, with the aim of increasing the transparency of animal research to wider publics. Yet, how this push for openness is experienced by those responsible for the care and welfare of research animals requires further study. This paper draws upon qualitative interviews with Named Veterinary Surgeons (NVS) in the UK and explores how they practise openness, avoid openness, and, at times, challenge the way their role is represented within openness agendas. Overall, this social scientific analysis reveals that the current openness agenda has the potential to create tensions for professionals, as they seek to manage regulatory and public imaginaries of the veterinary identity alongside the animal research controversy. The paper concludes by arguing for a culture of dialogue, where openness includes allowing those with responsibilities for animal welfare to express ambivalence or concern about their own role. Finally, the paper calls for sustained academic work on relations between the veterinary profession and wider society, particularly areas that involve contested practices in which care and harm may coincide.

Russell and Burch’s 1959 original definitions of the 3Rs (replacement, reduction and refinement) are widely used today as standards for the ethical use of non-human animals in research, although they have a number of limitations. Authors and institutions around the world have addressed some of these, coming up in certain cases with more accurate, functional, and up-to-date definitions. However, not only do there still remain limitations needing to be addressed, but some that have been addressed resulted in discrepancies, contradictions, and general confusion as to how best apply the 3Rs in practice. In order to clarify the meaning of the 3Rs and enable more optimal implementation of these principles in animal experimentation, this article provides a theoretical discussion for revised definitions of the original 3Rs via examination of some of their main limitations and inconsistencies. First, we offer up the original definitions as presented in the context of Russell and Burch’s book The Principles of Humane Experimental Technique. Then, we examine the main limitations and present clear specifications and requirements for such revised definitions. After presenting our revised definitions, we conclude with various implications for animal welfare within the context of experimentation.

An animal welfare education community of practice (AWECoP) for those teaching animal welfare science, applied ethology, and/or animal ethics was created to develop a dialogue amongst educators within the field of animal welfare science. The purpose of this paper is to describe the history, objectives, and members’ experiences within this community. AWECoP hosts 6–8 meetings annually for members to discuss topics relevant to our community and exchange teaching resources; within its first two years, the community has grown to 121 members representing approximately 70 institutions across six continents. A 12-question, mixed-method survey was distributed to capture member demographics, engagement with AWECoP, motivations for joining, and self-evaluation of AWECoP’s impacts. Quantitative data from the survey are presented descriptively, while reflexive thematic analysis was applied to the qualitative data. Survey respondents (n = 54) felt that AWECoP is a vital community and safe space for members to share their ideas and receive feedback, inspiration, information, and resources regarding subject-specific and broader pedagogical topics. As a result, a majority experienced professional (e.g. development of new contacts) and personal (e.g. increased feeling of belonging in their field) benefits, as well as impacts realised in their teaching practice. We conclude with an examination of challenges faced in ensuring AWECoP remains accessible to a growing membership and offer recommendations for facilitating similar communities to support fellowship and training in the teaching of animal welfare and related disciplines.