Following their early domestication, broomcorn millet and rice (in East Asia) and wheat and barley (in South-west Asia) were subsequently adopted across Eurasia during the Bronze Age/early historic period. The precise timing and dispersal routes for this trans-Eurasian exchange, however, remain unclear. Here, the authors present archaeobotanical evidence from sites on the Caspian Sea's southern coast, demonstrating that broomcorn millet reached West Asia by c. 2050 BC and rice by c. 120 BC. These dispersals relate to two waves of globalisation and were based on two different mechanisms: an ‘infiltration’ model (broomcorn millet) and a ‘leapfrog’ model (rice). The results contribute to our understanding of the continental-scale connectivity of the late prehistoric/early historic periods.

Laboratory studies were conducted to determine the ability of the extenders dietholate and SC-0058 to prevent enhanced biodegradation of EPTC and butylate applied to Wisconsin soils with different histories of carbamothioate herbicide use. Enhanced EPTC and butylate biodegradation occurred in soils previously treated with those herbicides. Enhanced biodegradation of dietholate occurred on soils previously treated with that extender plus either EPTC or butylate. Enhanced dietholate biodegradation was observed when applied alone or in combination with butylate or EPTC. Application with dietholate prevented enhanced biodegradation of butylate but not EPTC even though enhanced biodegradation of dietholate was occurring. Enhanced biodegradation of SC-0058 did not occur. SC-0058 prevented enhanced EPTC and butylate biodegradation even in soils previously treated for three consecutive years with the respective herbicide plus SC-0058. Application of either 1.1 or 2.2 kg/ha SC-0058 plus 6.7 kg/ha EPTC provided equal or better wild proso millet control and sweet corn yields than applications of EPTC alone or EPTC plus dietholate.

Efficacy of primisulfuron and reduced rates of nicosulfuron on woolly cupgrass, wild-proso millet, and giant foxtail in field corn was evaluated in 1994 and 1995. Nicosulfuron was applied at 18, 27, and 36 g ai/ha alone and with 20 g ai/ha primisulfuron. Woolly cupgrass control from nicosulfuron at 18 and 27 g/ha was less than control from 36 g/ha (79% and 84% vs. 90%, respectively). Woolly cupgrass control was 5% greater when primisulfuron was combined with nicosulfuron at 27 and 36 g/ha than when nicosulfuron was applied alone. Corn grain yields were similar among all nicosulfuron plus primisulfuron treatments applied to control woolly cupgrass in 1994. In 1995, corn grain yield was increased 900 kg/ha when primisulfuron was combined with nicosulfuron at 18 g/ha compared to nicosulfuron applied at the same rate alone. Wild-proso millet control 3 WAT with nicosulfuron at 18 g/ha was less than control with nicosulfuron at 36 g/ha. Wild-proso millet control 8 WAT with nicosulfuron alone was greater than when nicosulfuron at any rate was applied with primisulfuron. No differences in corn grain yields were observed across nicosulfuron rates or combinations with primisulfuron to control wild-proso millet either year. Giant foxtail control and corn grain yield were not affected by nicosulfuron rate or combined applications of nicosulfuron and primisulfuron either year.

DPX-79406 was evaluated for POST annual grass weed control in both controlled environment and field experiments. In controlled environment experiments, green foxtail was most susceptible to DPX-79406; whereas yellow foxtail was least susceptible of the species evaluated. DPX-79406 at 12 g/ha completely controlled six leaf black-seeded proso millet, yellow foxtail, green foxtail, and barnyardgrass. In the field, DPX-79406 at 3.0 to 25.0 g/ha effectively controlled annual grass weeds without injury to three- to six-leaf corn. There was more variation in the effectiveness of DPX-79406 applied in the field. Early POST applications provided less weed control than the late application, especially for barnyardgrass, because of weeds emerging after application. As a result, higher doses were sometimes needed for effective control. In weed-free field trials at two sites in 1990 and 1991, corn tolerated doses up to 75 g/ha of DPX-79406 applied at the three- to six-leaf growth stage. However, doses as low as 18.8 g/ha applied at the six- to nine-leaf growth stage reduced grain yield. In 1991, corn tillering increases and height and yield reductions were related linearly to the dose of DPX-79406 applied during later growth stages. DPX-79406 should be applied early POST in order to avoid crop injury while providing effective weed control.

Under simulated no-till conditions in the greenhouse, rye (Secale cereale L. ‘Wheeler’) residues reduced emergence of lettuce (Lactuca sativa L. ‘Ithaca’) and proso millet (Panicum miliaceum L. # PANMI) by 58 and 35%, respectively, over that obtained with a wood shaving control mulch. Rye shoot tissue inhibited lettuce germination 52% more than root tissue. Petri dish bioassays of residue in soil confirmed phytotoxicity on cress (Lepidium sativum L. ‘Curly’), lettuce, barnyardgrass (Echinochloa crus-galli L. Beauv. # ECHCG), and proso millet. Radicle elongation was a more senstive measure of phytotoxicity than germination per se. Phytotoxicity increased as the distance from seeds to rye residues decreased. While rye shoot residues caused 40% greater inhibition of cress radicle elongation in sterile soil than nonsterile soil, barnyardgrass was equally inhibited in both soil regimes. Phytotoxicity decreased in nonsterile soil, indicating that the compounds were degraded by microorganisms. Phytotoxic compounds in shoots were water extractable. Cress and barnyardgrass responded similarly to both aqueous rye extracts and to residues added to soil.

Field studies were conducted at Arlington, WI, in 1996 and 1997 and at Georgetown, DE, and LeSueur, MN, in 1997 to determine weed control efficacy, crop injury, and yield response of PAT-transformed sweet corn to glufosinate-based weed management. Sequential applications of glufosinate 10 to 18 d apart at 0.4 and 0.3 kg ai/ha controlled common lambsquarters, common ragweed, velvetleaf, wild-proso millet, and fall panicum 90% or better at all locations. Weed control varied little among 0.3, 0.4, or 0.3 and 0.3 (sequential) kg/ha glufosinate rates. Glufosinate applied alone, with, or following atrazine controlled velvetleaf 90% or greater but was less consistent on common ragweed and common lambsquarters (73 to 100%). Atrazine plus metolachlor applied preemergence (PRE) and glufosinate applied alone postemergence (POST) provided inconsistent wild-proso millet and fall panicum control (43 to 99%). Metolachlor followed by glufosinate improved consistency of grass control (> 76%). Glufosinate followed by cultivation provided 80% or greater control of velvetleaf and wild-proso millet. Glufosinate did not injure or delay maturity of PAT-transformed sweet corn. Sweet corn treated with glufosinate resulted in yields greater than or equal to the sweet corn that was hand-weeded or received a standard herbicide treatment.

Herbicide efficacy trials in field corn, sweet corn, and soybean were conducted at three locations in Wisconsin over a 6-yr period. Percent weed pressure (WP) was determined by visually estimating the contribution of all weed species present to the total crop and weed volume in each plot. Crop yields in each plot were measured. Percent crop yield reduction (YLDRED) was calculated by comparing mean yields of individual treatments with those of the highest yielding treatment in each trial. Linear regression analyses of YLDRED and WP data from 1640 field corn and 138 sweet corn treatments were significant. Nonlinear regression analysis of YLDRED and WP data from all 1374 soybean treatments was significant; however, a linear regression of those 1154 soybean treatments with WP ratings of 30 or less produced a more easily interpreted regression equation.

Weed seeds were germinated in incubators at constant temperatures ranging from 4 to 32 C increments. Percent germination per day was regressed against temperature with the x-intercept representing the low threshold temperature for development. Low threshold temperatures for development (low TTD's) calculated for wild proso millet (Panicum miliaceum L. # PANMI), barnyardgrass [Echinochloa crus-galli (L.) Beauv. # ECHCG], common lambsquarters (Chenopodium album L. # CHEAL), and redroot pigweed (Amaranthus retroflexus L. # AMARE) were 6.9, 9.7, 6.0, and 10.0 C, respectively.

A four-year experiment was conducted near Scottsbluff, NE to evaluate the effect of different methods of preplant tillage, post-plant cultivation, and herbicides on weed density and grain yield. Ridge-till enhanced the development of kochia and reduced the density of wild proso millet and common lambsquarters. Tandem disking increased longspine sandbur and redroot pigweed density whereas moldboard plowing increased common sunflower density. Cultivation reduced weed density 86% but weeds that remained after cultivation reduced corn grain yield by 40% as compared with a hand-weeded control. Weed control treatments that integrated cultivation and herbicides controlled a broader spectrum of weed species than cultivation or herbicide treatments alone.

The majority of the early crops grown in Europe had their origins in south-west Asia, and were part of a package of domestic plants and animals that were introduced by the first farmers. Broomcorn millet, however, offers a very different narrative, being domesticated first in China, but present in Eastern Europe apparently as early as the sixth millennium BC. Might this be evidence of long-distance contact between east and west, long before there is any other evidence for such connections? Or is the existing chronology faulty in some way? To resolve that question, 10 grains of broomcorn millet were directly dated by AMS, taking advantage of the increasing ability to date smaller and smaller samples. These showed that the millet grains were significantly younger than the contexts in which they had been found, and that the hypothesis of an early transmission of the crop from east to west could not be sustained. The importance of direct dating of crop remains such as these is underlined.

Early farming in northern China featured the cultivation of two species of millet, broomcorn and foxtail. Although previously seen as focused on the Yellow River, the authors show that the earliest agriculture is actually found in the foothills of the neighbouring mountain chains, where drier and better drained locations suited millet cultivation, particularly broomcorn. In this they echo new thoughts on the locale of early agriculture in south-west Asia, on the hilly flanks of the Fertile Crescent rather than in the valleys of the Nile or the Euphrates.

Seed remains of common millet (Panicum miliaceum L.) were excavated from sites of ad 4th-century Darhan (Mongolia), and ad 15th-century Budapest (Hungary). Because the 15th-century medieval grains looked so intact, a germination test was carried out under aseptic conditions, which resulted in swelling of the grains but no cell proliferation or germination. Ancient DNA (aDNA) was extracted from the aseptic grains; analysed for amplified fragment length polymorphisms (AFLP), simple sequence repeats (SSR) and mitochondrial DNA (mtDNA); and compared with the modern millet cultivar ‘Topaz’. AFLP analysis revealed that extensive DNA degradation had occurred in the 4th-century ancient millet, resulting in only 2 (1.2%) AFLP fragments (98.8% degradation) amplified by MseCAA–EcoAGT, compared to the 15th-century medieval millet, with 158 (40%) fragments (60% degradation), and modern millet cultivar ‘Topaz’ with 264 fragments (100%). EcoAGT–MseCAA was found to be the most effective selective-primer combination for the analysis of medieval and modern millet. Eight AFLP fragments were sequenced after re-amplification and cloning. Microsatellite (SSR) analysis at the nuclear gln4, sh1, rps28 and rps15 loci revealed one SNP (single nucleotide polymorphism) at the 29th position (A→G) of rps28 locus, compared to modern millet. An mtDNA fragment (MboI), amplified at the 18S–5S ribosomal DNA (rDNA) locus in the medieval millet, showed no molecular changes compared to modern millet. The results underline the significance of aDNA extraction and analysis of excavated seeds for comparative analysis and molecular reconstruction of ancient and extinct plant genotypes.