Gene expression studies in organisms are often conducted using reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR), and the accuracy of RT-qPCR results relies on the stability of reference genes. We examined ten candidate reference genes in Sclerodermus guani, a parasitoid wasp that is a natural enemy of long-horned beetle pests in forestry, including ACT, EF1α, Hsc70, Hsp70, SRSF7, α-tubulin, RPL7A, 18S, 28S, and SOD1, regarding variable biotic and abiotic factors such as body part, life stage, hormone, diet, and temperature. Data were analysed using four dedicated algorithms (ΔCt, BestKeeper, NormFinder, and geNorm) and one comparative tool (RefFinder). Our results showed that the most stable reference genes were RPL7A and EF1α regarding the body part, SRSF7 and Hsc70 regarding the diet, RPL7A and α-tubulin regarding the hormone, SRSF7 and RPL7A regarding the life stage, and SRSF7 and α-tubulin regarding temperature. To ascertain the applicability of specific reference genes, the expression level of the target gene (ACPase) was estimated regarding the body part using the most stable reference genes, RPL7A and EF1α, and the least stable one, SOD1. The highest expression level of ACPase was observed in the abdomen, and the validity of RPL7A and EF1α was confirmed. This study provides, for the first time, an extensive list of reliable reference genes for molecular biology studies in S. guani.

This paper proves normalisation theorems for intuitionist and classical negative free logic, without and with the operator for definite descriptions. Rules specific to free logic give rise to new kinds of maximal formulas additional to those familiar from standard intuitionist and classical logic. When is added it must be ensured that reduction procedures involving replacements of parameters by terms do not introduce new maximal formulas of higher degree than the ones removed. The problem is solved by a rule that permits restricting these terms in the rules for $\forall $, $\exists $ and to parameters or constants. A restricted subformula property for deductions in systems without is considered. It is improved upon by an alternative formalisation of free logic building on an idea of Jaśkowski’s. In the classical system the rules for require treatment known from normalisation for classical logic with $\lor $ or $\exists $. The philosophical significance of the results is also indicated.

Since the inauguration of Mexican democracy in 2000, organised criminal violence had been leaking into the political arena. Yet, it escalated in the 2018 elections, when dozens of local candidates were killed. In most of these cases, the concrete perpetrators and motives remained in the dark. How did Mexican society make sense of this opaque, unprecedented wave of electoral violence? On the basis of a qualitative content analysis of over 1,200 news reports, I examine the structuring power of a shared narrative: the frame of organised crime. By conceiving candidate killings as economic violence within the criminal community, this commonsensical frame of interpretation permitted Mexican society to ‘normalise’ these killings as ‘business as usual’ by criminal organisations.

The line connecting rare earth elements (REE) in chondrite-normalised plots can be represented by a smooth polynomial function using λ shape coefficients as described by O'Neill (2016). In this study, computationally generated λ combinations are used to construct artificial chondrite-normalised REE patterns that encompass most REE patterns likely to occur in natural materials. The dominant REE per pattern is identified, which would lead to its inclusion in a hypothetical mineral suffix, had this mineral contained essential REE. Furthermore, negative Ce and Y anomalies, common in natural minerals, are considered in the modelled REE patterns to investigate the effect of their exclusion on the relative abundance of the remainder REE. The dominant REE in a mineral results from distinct pattern shapes requiring specific fractionation processes, thus providing information on its genesis. Minerals dominated by heavy lanthanides are rare or non-existent, even though the present analysis shows that REE patterns dominated by Gd, Dy, Er and Yb are geologically plausible. This discrepancy is caused by the inclusion of Y, which dominates heavy REE budgets, in mineral name suffixes. The focus on Y obscures heavy lanthanide mineral diversity and can lead to various fractionation processes to be overlooked. Samarium dominant minerals are known, even though deemed unlikely by the computational model, suggesting additional fractionation processes that are not well described by λ shape coefficients. Positive Eu anomalies only need to be moderate in minerals depleted in the light REE for Eu to be the dominant REE, thus identifying candidate rocks in which the first Eu dominant mineral might be found. Here, I present an online tool, called ALambdaR that allows interactive control of λ shape coefficients and visualisation of resulting REE patterns.

L’objet du travail est de montrer l’intérêt d’une application à la psychiatrie du concept de normativité, développé initialement dans le cadre général de la médecine par G. Canguilhem. Méthodologiquement, c’est une recherche de philosophie appliquée. La médecine recourt à diverses disciplines pour produire des déterminations à sa pratique, – d’où la terminologie plurielle et équivoque de « sciences médicales ». Il n’est donc pas illégitime, à certaines conditions, de produire une recherche philosophique sur la médecine. À partir d’une lecture de Canguilhem, nous avons tâché d’expliciter le concept de normativité en ce qui concerne la maladie dans sa dimension organique. Ce concept détermine la nature de la différence axiologique entre le normal et le pathologique. En introduisant les notions de valeur et de finalité, il se montre fécond à orienter la pratique clinique médicale, par-delà les objectivations scientifiques et technologiques. Nous avons proposé d’appliquer ce concept à la notion de maladie mentale. Les résultats de cette application montrent que la maladie mentale relève d’une même logique que celle de la maladie organique, ce qui permet de garantir la place de la psychiatrie dans le champ de la médecine. Néanmoins, cette logique n’opère pas dans le même contexte. À partir d’une même normativité, organisme et psychisme apparaîssent comme les deux modes d’individualisation de l’être humain : d’abord dans l’environnement naturel (champ de lois) par le caractère générique d’espèce, ensuite, dans un monde social et culturel (champ de normes) par le caractère particulier de la personnalité. De cette double détermination impliquant les rapports, répulsifs ou propulsifs, de l’individu normatif au milieu, il découle une définition plurivoque mais synthétique de la maladie et de la santé. La spécificité de la psychiatrie peut être alors précisée. Dans cette perspective, se dégage un programme de philosophie appliquée à la psychiatrie, à la médecine et à leurs rapports.

Part-of-speech (PoS) tagging of non-standard language with models developed for standard language is known to suffer from a significant decrease in accuracy. Two methods are typically used to improve it: word normalisation, which decreases the out-of-vocabulary rate of the PoS tagger, and domain adaptation where the tagger is made aware of the non-standard language variation, either through supervision via non-standard data being added to the tagger’s training set, or via distributional information calculated from raw texts. This paper investigates the two approaches, normalisation and domain adaptation, on carefully constructed data sets encompassing historical and user-generated Slovene texts, in particular focusing on the amount of labour necessary to produce the manually annotated data sets for each approach and comparing the resulting PoS accuracy. We give quantitative as well as qualitative analyses of the tagger performance in various settings, showing that on our data set closed and open class words exhibit significantly different behaviours, and that even small inconsistencies in the PoS tags in the data have an impact on the accuracy. We also show that to improve tagging accuracy, it is best to concentrate on obtaining manually annotated normalisation training data for short annotation campaigns, while manually producing in-domain training sets for PoS tagging is better when a more substantial annotation campaign can be undertaken. Finally, unsupervised adaptation via Brown clustering is similarly useful regardless of the size of the training data available, but improvements tend to be bigger when adaptation is performed via in-domain tagging data.

This article assesses how social innovations in the field of local domiciliary long-term care are shaped and implemented. It proposes a mapping of innovations in terms of two structuring discourses that inform welfare state reforms: a libertarian and a neo-liberal discourse. It then provides an analysis of the concrete trajectories of three local innovations for elderly people in Hamburg (Germany), Edinburgh (Scotland) and Geneva (Switzerland). Theoretically, social innovation is considered as a discursive process of public problem redefinition and institutionalisation. New coalitions of new actors are formed along this double process, and these transform the original discourse of innovation. The comparative analysis of the three processes of institutionalisation of local innovation shows that, in the context of local policy making, social innovations inspired by a libertarian critique of the welfare state undergo differentiated processes of normalisation.

This study was aimed at providing normative data for the Mini-Mental State Examination (MMSE). The norms were built from a sample (n = 2409) of community-dwelling French speaking residents from Québec aged 65 and older. The analyses indicated that socio-demographic variables such as education level, age, and gender of individuals influenced significantly the scores of older adults on the MMSE. More precisely, MMSE scores increased with education level and decreased with age. Moreover, women had significantly higher scores than men. On this basis, distinct tables of normative data were produced for women and men. In each table, the MMSE scores corresponding to percentiles 5, 10, 15 and 50 were identified according to four age categories and three education levels. Overall, the use of the present normative data by clinicians will improve their accuracy in detecting cognitive impairment in older adults from Québec.

People with learning difficulties are now surviving into old age and more and more of them are being resettled from long-stay hospitals. The main purpose of this article is to examine some of the key barriers facing, and dilemmas involved in, the provision of community-based services to this newly emerging user group. The findings of some recent field research on older people with learning difficulties who have been resettled into the community are used to illustrate the challenges facing service providers. Particular reference is made to the impact of age discrimination and traditional differences between service specialisms. The article shows that both official definitions of need in old age and the aspirations of the service responses to those needs have been artificially constructed in very narrow terms, especially when compared with the principled approach to service provision for younger people with learning difficulties. In conclusion, therefore, it is argued that the principles underpinning services for people with learning difficulties, such as normalisation, should be applied to older people as well.