A growth monitoring study (0–7 day of age) was conducted involving 87, one-day old Ross 308 male broilers to evaluate organ weights, bone parameters and ileal transcriptomic profile of broiler chicks as influenced by day 7 bodyweight (BW) grouping. The chicks were raised in a deep-litter house under common controlled environmental conditions and commercial starter diet. Chicks were grouped on day 7 into two distinct BW, super performer (SP) and under performer (UP) with bodyweights >260, and <200 g respectively. Results revealed that the SP chicks had significantly higher bone ash, sodium (Na), phosphorus (P) and rubidium (Rb) concentrations compared to the UP chicks on D7. In contrast, the UP chicks had significantly higher tibial cadmium (Cd), caesium (Cs) and lead (Pb) compared to the SP group; the UP chicks also had proportionally heavier relative gizzard weight than the SP chicks. The ileal transcriptomic data revealed differentially expressed genes (DEG) between the two groups of chicks, with 150 upregulated and 83 down-regulated genes with a fold change of ≥1.25 or ≤ 1.25 in the SP chicks relative to the UP chicks. Furthermore, functional annotation and pathway analysis revealed that some of these DEG were involved in various pathways including calcium signalling, Wnt signalling, cytokine-cytokine receptor interaction and mucin type O-glycan biosynthesis. This study revealed that chicks of the same breed and of uniform environmental and diet management exhibited differences in digestive organ weights, tibial bone characteristics and ileal gene expression that may be related to BW.

Postprandially, amino acids and di/tripeptides are thought to be primarily absorbed in the proximal small intestine. However, there have been no in vivo demonstrations of regional differences in amino acid transport dynamics between the proximal and distal small intestines. We monitored plasma amino acid responses in the jejunal and ileal mesenteric veins of rats after oral administration of a diet or an amino acid mixture (Experiment 1), and in the portal vein after direct administration of the amino acid mixture into the lumen of the jejunum or ileum (Experiment 2). In experiment 1, the total and some amino acid concentrations in the jejunal mesenteric vein were slightly higher than those in the ileal mesenteric vein after oral administration of the amino acid mixture, suggesting that the ileum actively transports luminal amino acids to the basolateral side, comparable to the jejunum. In experiment 2, portal amino acid concentrations were elevated to a greater extent after direct administration of the amino acid mixture into the ileal lumen than into the jejunal lumen. These results demonstrate regional differences in amino acid transport dynamics in vivo and suggest that the ileum has a higher capacity for transporting amino acids than the jejunum. Our findings highlight the importance of the ileum in postprandial amino acid absorption and metabolism.

This study investigated the effects of Lacticaseibacillus rhamnosus HN001 supplementation on the architecture and gene expression in small intestinal tissues of piglets used as an animal model for infant humans. Twenty-four 10-d-old entire male piglets (4·3 (sd 0·59) kg body weight) were fed an infant formula (IF) (control) or IF supplemented with 1·3 × 105 (low dose) or 7·9 × 106 (high dose) colony-forming units HN001 per ml of reconstituted formula (n 8 piglets/treatment). After 24 d, piglets were euthanised. Samples were collected to analyse the histology and gene expression (RNAseq and qPCR) in the jejunal and ileal tissues, blood cytokine concentrations, and blood and faecal calprotectin concentrations. HN001 consumption altered (false discovery rate < 0·05) gene expression (RNAseq) in jejunal tissues but not in ileal tissues. The number of ileal goblet cells and crypt surface area increased quadratically (P < 0·05) as dietary HN001 levels increased, but no increase was observed in the jejunal tissues. Similarly, blood plasma concentrations of IL-10 and calprotectin increased linearly (P < 0·05) as dietary HN001 levels increased. In conclusion, supplementation of IF with HN001 affected the architecture and gene expression of small intestine tissue, blood cytokine concentration and frequencies, and blood calprotectin concentrations, indicating that HN001 modulated small intestinal tissue maturation and immunity in the piglet model.

Excess iodine intake by the pregnant dam reduces lamb serum antibody concentration, specifically immunoglobulin G (IgG). An experiment was conducted to investigate the mechanisms under pinning the reduced serum IgG concentration at 24 h postpartum in the progeny of iodine supplemented dams. Forty-five mature twin bearing ewes (n=15/treatment) were allocated to one of three dietary treatments as follows: basal diet (Control); basal diet plus 26.6 mg of iodine per ewe per day as calcium iodate (CaIO3); or potassium iodide (KI). Ewes were individually housed and fed from d 119 of gestation until parturition. All lambs received colostrum at 1, 10 and 18 h postpartum via stomach tube. At 1 h postpartum lambs from the control and an iodine supplemented treatment (n=10 per treatment from control and CaIO3) were euthanised before colostrum consumption and ileal segments isolated to determine the gene expression profile of a panel of genes identified as having a role in antibody transfer. Preceding euthanasia, lambs were blood sampled for determination of serum IgG, total thyroxine and free tri-iodothyronine concentrations. Progeny of CaIO3 supplemented dams had lower tri-iodothyronine concentrations (P<0.01) at 1 h postpartum and lower serum IgG concentrations (P<0.001) at 24 h postpartum when compared with the progeny of control dams. Iodine (CaIO3) supplementation of the dam increased the relative expression (P<0.05) of the B2M, PIGR and MYC genes in the ileum of the lamb, before colostrum consumption; while the expression of THRB declined when compared with the progeny of C dams (P<0.01). In conclusion, the results of this study show that it is the actual inclusion of excess iodine in the diet of the ewe, regardless of the carrier element, that negatively affects passive transfer in the newborn lamb. This study presents novel data describing the relationship between maternal iodine nutrition and its effect on the thyroid hormone status and subsequent gene expression in the newborn lamb; which results in a failure of passive transfer and a decline in serum IgG concentration.

Bumblebees need to keep bodily homeostasis and for that have an efficient system of excretion formed by the Malpighian tubules, ileum, and rectum. We analyzed the excretory organs of Bombus morio, a bee without rectal pads. In addition, we analyzed the rectal epithelium of Melipona quadrifasciata anthidioides which has rectal pads. The Malpighian tubules exhibited two cell types and the ileum four types. However, comparative analysis of the rectum showed that only cells of the anterior region of the rectal epithelium of B. morio are structurally distinct. We suggest that cells of the Malpighian tubules of B. morio have an excretory feature and that cells of ileum have different functions, such as ion absorption and water, organic compound, and protein secretion. In addition, only the anterior region of the rectum of B. morio showed characteristic absorption. We suggest that Malpighian tubules participate in the excretion of solutes and that the ileum and rectal epithelium are responsible for homeostasis of water and solutes, compensating for the absence of rectal papillae. These results contribute to our understanding of the morphophysiology of the excretory organs of bees without rectal pads.

An adequate immune system is required to prevent diarrhoea in neonates, and IgA provides protection against microbial antigens on mucosal surfaces. Although β-carotene supplementation has been expected to enhance the retinoic acid (RA)-mediated immune response in neonates, the exact mechanism of the enhancement of mucosal IgA production in the small intestine by β-carotene is still unclear. In the present study, we investigated the effect of supplemental β-carotene on the concentrations of IgA, the numbers of IgA antibody-secreting cells (ASC) and the mRNA expressions of IgA C-region, CCL25, retinoid X receptor (RXR) α, retinoic acid receptor (RAR) α and RARγ in the jejunum and ileum of weanling mice. Weanling mice were fed rodent feed or 50 mg/kg β-carotene-supplemented rodent feed for 7, 14 or 21 d. The concentrations of IgA and the numbers of IgA ASC in the jejunum and ileum of mice increased markedly with age, and supplemental β-carotene increased the concentrations of IgA, the numbers of IgA ASC and the mRNA expressions of IgA C-region, CCL25 and RARγ in the jejunum after 14 and 21 d of treatment. Supplemental β-carotene increased the numbers of IgA ASC in the ileum after 14 and 21 d of treatment, but the concentrations of IgA in the ileum were not affected by β-carotene supplementation. The mRNA expressions of RXRα and RARα in the jejunum and those of RXRα and RARγ in the ileum after 21 d of treatment were enhanced by β-carotene supplementation. These results indicate that β-carotene supplementation in weanling mice is effective to enhance mucosal IgA induction in the jejunum or ileum and that the effects are mainly due to the RA-mediated immune response.

The aim of the present study was to establish the optimum inclusion level of laminarin derived from Laminaria digitata on selected microbial populations, intestinal fermentation, cytokine and mucin gene expression in the porcine ileum and colon. A total of twenty-one pigs (mean body weight 17·9 kg) were randomly assigned to one of three dietary treatments: T1 – basal (control) diet, T2 and T3 – basal diets supplemented with laminarin included at 300 and 600 parts per million (ppm), respectively. Selected intestinal bacterial populations and volatile fatty acid (VFA) concentrations were measured in the ileum and colon. Relative gene expression levels for specific cytokine and mucin genes were investigated in ileal and colonic tissue in the absence and presence of a lipopolysaccharide (LPS) challenge. There was an up-regulation of MUC2 gene expression at the 300 ppm inclusion level in the ileum. In the colon, there was a significant reduction in the enterobacteriaceae population at the 300 ppm inclusion level (P = 0·0421). Dietary supplementation of 600 ppm laminarin led to a significant increase in MUC2 (P = 0·0365) and MUC4 (P = 0·0401) expression in the colon, and in the total VFA concentration in the caecum (P = 0·0489). A significant increase was also recorded in IL-6 (P = 0·0289) and IL-8 gene expression (P = 0·0245) in LPS-challenged colonic tissue at both laminarin inclusion levels. In conclusion, dietary inclusion of 300 ppm laminarin appears to be the optimum dose in the present study due to the reduction in the enterobacteriaceae populations and enhanced IL-6 and IL-8 cytokine expression in response to an ex vivo LPS challenge.

These studies were conducted to determine whether dietary homoarginine, derived from guanidination of synthetic lysine, affects the measurement of apparent and true ileal amino acid digestibility in the pig and to determine the optimal ratio of o-methylisourea (OMIU) to free synthetic lysine in the guanidination reaction that produces homoarginine. In two preliminary experiments, specific amounts of lysine were weighed and added to a solution of 0·6 mol/l OMIU to produce OMIU to free synthetic lysine ratios of 2:1, 1·5:1, 1·4:1, 1·3:1, 1·2:1, 1·1:1 and 1·0:1. The mixture was incubated at a pH of 10·5 and a temperature of 20±1°C for 6 days. The highest yield of homoarginine (proportionately 0·995) was produced with an OMIU to free synthetic lysine ratio of 1·5:1. The serum concentrations of homoarginine and lysine were measured in two test pigs at various times after consuming a single meal containing either homoarginine or lysine. Blood samples were taken before and 0·5, 1·5, 2·5, 4·5, 6·0, 7·5, and 11·5 h after feeding. Thirty minutes after feeding the homoarginine-containing diet, the homoarginine concentration in serum was 0·013 mmol/dl, and increased to a maximum of 0·031 mmol/dl 1·5 h after feeding. Thirty minutes after feeding the lysine-containing diet, the serum lysine concentration was 0·011 mmol/dl and also reached its maximum concentration of 0·025 mmol/dl 1·5 h after feeding. The timing of the changes in concentration of homoarginine and lysine related to feeding were similar, which suggests that homoarginine is absorbed and metabolized in a similar manner to lysine. Six Large White×Landrace barrows, weighing 68·5±3·5 kg and fitted with a simple T-cannula at the distal ileum as well as a jugular vein catheter, were used to determine the effects of homoarginine on endogenous amino acid flows and apparent and true ileal amino acid digestibility. The experimental design was a doubly replicated 3×3 Latin-square design with the three treatments consisting of a lysine-free, zein-based diet and jugular infusion of a lysine solution; a lysine-free, zein-based diet supplemented with homoarginine; and a lysine-free, zein-based diet supplemented with l-lysine. There were no significant differences in endogenous ileal lysine flow for pigs receiving the different treatments (396·6, 407·6, and 360·4 mg/kg DMI, respectively, P=0·71). In addition, with the exception of phenylalanine (P=0·04), there were no significant differences (P>0·10) in the apparent and true ileal digestibility of crude protein and amino acids among the three treatments. Therefore, it would appear that the presence of dietary homoarginine, derived from guanidination of synthetic lysine, does not affect endogenous amino acid losses or the digestibility of crude protein as well as most amino acids. These results are therefore supportive of the use of the homoarginine method as a means of measuring true ileal lysine digestibility.

Five castrated male pigs (100±2 kg mean live weight) fitted with T-shaped ileal cannulae were used to determine ileal digestibility of raw or autoclaved (136°C, 3 min, 2·3 bar) kidney-bean seed meals, which were included (536 g/kg) in the diet as the only protein source. A protein-free and a diet based on hydrolysed casein were used to estimate endogenous protein secretion. Apparent ileal digestibility of dry matter was lower (P < 0·05) for raw compared to autoclaved kidney-bean meal. Apparent and true ileal digestibilities of raw kidney-bean meal were not only lower (P < 0·05) than those of autoclaved kidney beans, but negative or close to zero for N and some amino acids. Ileal N (36·9 g/kg food ingested) and sialic acid (3·5 g/kg food ingested) flows in pigs given the raw kidney-bean-based diet were higher (P < 0·05) than those of pigs given the autoclaved kidney-bean diet (respectively 8·9 and 1·4 g/kg food ingested). Autoclaved kidney beans contained 364 and 143 g/kg of starch and non-starch polysaccharides respectively, the ileal digestibilities of which were 0·79 and 0·26. Among individual non-starch polysaccharide sugars, apparent ileal digestibility values were between 0 (rhamnose) and 0·38 (arabinose). Ileal apparent digestibility of total oligosaccharides in the autoclaved kidney bean diet was 0·52, and total amounts of oligosaccharides digested was 18·6 g/kg food. Among individual short-chain fatty acids in ileal contents, only propionate values were signifi cantly (P < 0·05) different between pigs given raw or autoclaved kidney-bean diets. Lactate concentration in ileal contents was higher (P < 0·05) in pigs given autoclaved compared with raw kidney beans. Ileal digestibility of autoclaved kidney-bean meal in the 10-kg pigs was poorer than that of other raw legume-seed meals such as lupins and chickpeas in other studies. The potential health implications of these results are also discussed.

The apparent ileal digestibility coefficients of amino acids in 107 samples representing 22 food ingredients were determined using 6-week-old broiler chickens. The ingredients assayed included five cereals (barley, maize, sorghum, triticale and wheat), two cereal by-products (rice polishings and wheat middlings), four oilseed meals (canola, cottonseed, soya-bean and sunflower meals), full-fat canola, maize gluten meal, four grain legumes (chickpeas, faba beans, field peas and lupins) and five animal protein sources (blood, feather, fish, meat and meat and bone meals). The mean ileal digestibility coefficients of amino acids in wheat and maize were higher than those in sorghum, triticale and barley. However, variations observed in individual amino acid digestibilities among samples within cereal type were greater than those determined between cereals. Threonine and lysine were the least digestible indispensable amino acids in the five cereals evaluated. The most digestible indispensable amino acid was phenylalanine in wheat and, leucine in maize and sorghum. In the case of the wheat middlings and rice polishings, threonine was the least digestible indispensable amino acid and arginine was the best digested.

In the oilseed meals assayed, amino acid digestibility was highest for soya-bean and sunflower meals, intermediate for canola meal and lowest for cottonseed meal. Ileal digestibility coefficients of amino acids in lupins were found to be slightly lower than those in soya-bean meal. The amino acid digestibilities of field peas, faba beans and chickpeas were considerably lower than those of lupins. Digestibility of arginine was the highest and that of threonine was the lowest of the indispensable amino acids in oilseed meals and grain legumes, except in cottonseed meal. Lysine was the least digestible amino acid in cottonseed meal.

In the animal protein sources assayed, digestibility coefficients of amino acids in blood meal were high, intermediate in fish meal, and low in meat meal, meat and bone meal and feather meal. Variation in amino acid digestibility coefficients determined for blood meal samples was small. However, wide variations in amino acid digestibilities were observed for other animal protein sources, highlighting significant batch-to-batch differences. In particular, marked variations were determined for meat meal and meat and bone meal samples. Cystine was the least digested amino acid in animal protein meals, with the exception of blood meal in which isoleucine had the lowest digestibility. The limitations of using apparent digestibility values in diet formulations and the concept of the standardized digestibility system to overcome these limitations are discussed.

Endogenous flows of nitrogen and amino acids at the terminal ileum of broilers (6 weeks old), layers (70 weeks old) and adult roosters (70 weeks old) were determined using the peptide alimentation method. The ileal endogenous output of nitrogen and total amino acids in broilers, layers and roosters, expressed as mg/kg dry matter intake, were similar (F > 0-05). Endogenous flows were similar (F > 0-05) for nine of the 17 amino acids analysed, but the flows of serine, glutamic acid, proline, alanine, isoleucine, tyrosine, arginine and methionine differed (P < 0-05) among the classes of chickens. The amino acid profile of endogenous protein, expressed asg/100 g crude protein, did not differ (F > 0-05) between the three classes of chickens, except for serine, glutamic acid, proline and isoleucine. The concentrations of proline were higher (F < 0-05) in broilers, compared with the other two groups. The concentrations of glutamic acid in layers were lower (F < 0-05) than the other two groups. The concentrations of serine and isoleucine were higher (F < 0-05) in roosters than the other two groups. In all three groups, the most abundant amino acid in the ileal endogenous protein was glutamic acid, followed by aspartic acid, proline, serine, glycine and threonine. The present study provides estimates for endogenous amino acid flow at the terminal ileum in broilers, layers and adult roosters under normal physiological conditions.

The effect of including purified jack bean lectin (concanavalin A) in the diet of grower pigs on endogenous ileal nitrogen (N) and amino acid (AA) losses, and apparent and true ileal AA digestibility was investigated in a study with four growing pigs (average initial live weight (LW) 25 kg) fitted with simple T-cannulae at the terminal ileum. Both the homo-arginine (HA) method and peptide-alimentation method were used to estimate endogenous gut AA losses. The animals were offered four diets :(1) an enzymatically hydrolysed casein (EHC) containing diet, (2) an EHC containing diet supplemented with 266 mg/kg jack bean lectin, (3) a normal casein (NC) containing diet, and (4) a NC containing diet supplemented with 266 mg/kg lectin. For the estimation of ileal endogenous AA flows in pigs given NC diets, a subsample ofNC was guanidinated to convert lysine to HA. The experiment was conducted using a 4X4 Latin-square design. In each experimental period, pigs were adjusted to the experimental diets for 5 days. On day 6, ileal digesta was collected continuously for 24 hfor determination of apparent ileal digestibility. At 08:30 h of day 8 the HA NC diets were offered to pigs on NC treatments and ileal digesta were again collected for 24 h from all pigs. There were no interactive effects of casein type and added dietary lectin on apparent and true ileal AA digestibility and endogenous ileal AA flows (P > 0-05). The addition of lectin to the diet did not influence (P > 0-05) the apparent ileal digestibility of dry matter (DM), crude protein (CP) and AA. Casein type did not affect ileal DM digestibility (P > 0-05). Pigs given the NC-based diets had higher (P < 0-05) apparent ileal AA digestibility than pigs given the EHC-based diets. Addition of lectin to the diet did not influence (P > 0-05) endogenous ileal CP and AA flows. Endogenous ileal AA losses were generally higher (P < 0-05) in pigs given EHC diets than in pigs given NC diets. Neither casein type nor diet lectin level influenced (P > 0-05) the true ileal digestibility ofCP and AA; values ranged for most AA between 0-98 and 1-00. These data suggest that including purified jack bean lectin at the level of 266 mg/kg in a casein-based diet did not influence the ileal endogenous CP and AAflow, nor the apparent and the true ileal digestibility ofCP and AA, in growing pigs between 25 and 50 kg LW. Compared with the HA method, the peptide-alimentation method yielded higher estimates of ileal endogenous CP and AA acid losses in pigs given casein-based diets, although these two methods yielded similar estimates of true ileal AA and CP digestibility.

Endogenous ileal amino acid flows were determined in pigs fitted with simple T-cannulas using either the regression method (meat and bone meal (MBM) as the protein source at five levels of inclusion) or following protein-free alimentation. Amino acid flows were compared with those determined by feeding animals a diet the sole nitrogen source of which was enzyme-hydrolysed casein (EHC), followed by centrifugation and ultrafiltration of the ileal digesta. The EHC was a mixture of free amino acids and oligopeptides (molecular weight 5000 Da). For the EHC treatment, the ileal digesta precipitate plus retentate was used to determine the endogenous flows. The ultrafiltration step excludes unabsorbed dietary amino acids from the measure of endogenous loss. Chromium III oxide was the reference marker in all diets. Estimates of endogenous nitrogen and amino acid flows determined under protein-free alimentation and the comparable flows determined using the regression method were similar. However, endogenous flows of amino acids for the EHC-fed pigs were generally significantly higher (F < 0·01) than values found for pigs on the protein-free diet and were higher than values obtained after extrapolation for pigs given the MBM-based diets. Mean endogenous ileal nitrogen flow for the EHC-fed animals was 2526 (s.e. 33.9) compared with 1711 (s.e. 25.5) mg/kg dry-matter intake for pigs receiving the protein-free diet.

Previous studies on ageing animal and human subjects have demonstrated a significant overall decline in neuronal numbers in the myenteric plexus of the enteric nervous system (ENS). Our study aimed to confirm this observation by counting myenteric neurons stained with the panneuronal markers PGP 9.5 and NADH-diaphorase. We also wished to examine the possibility that particular subpopulations of neurons are vulnerable. Therefore, we have immunostained and counted a number of nerve cell groups within the myenteric plexus of old and young Sprague Dawley rats using markers which reflect some of the neuronal phenotypes present, including ChAT and VIP. The number of neurons demonstrating NADH-diaphorase activity was significantly reduced (P<0.05) by approximately 15% in old rats. However, the number of neurons stained for PGP 9.5 immunohistochemistry was not reduced and demonstrated larger numbers of neurons than the NADH-diaphorase method. None of the other neuronal markers studied showed any significant reductions with age. In contrast to previous work, this study has gathered little evidence for extensive cell loss in the myenteric plexus of the aged rat, either in overall populations, or in any of the principal functional groups of neurons.

An unusual case of laryngeal carcinoma metastasizing to the small intestine is reported. The patient was admitted to our hospital due to hoarseness, sore throat and dyspnoea. Pathological examination on the laryngeal mass revealed it to be moderately differentiated squamous cell carcinoma, staged as T4N0M0. A total laryngectomy and right modified neck dissection were performed. Over two years after the operation a small intestinal tumour was found, with a resultant resection of the ileum and sigmoid colon. An infiltration of the cell nests of squamous cell carcinoma were present at the submucosal regions of the ileum. These findings indicated that the laryngeal cancer had already metastasized to the small intestine at the time of the initial treatment.

The effect of condensed tannins (CT) from heated and unheated cottonseed on the apparent ileal digestibility of amino acids for the growing rat and pig was determined. In Expt 1, twenty-four rats were allocated to four semi-purified diets which contained cottonseed kernel and hulls as the only protein source. Two of the diets contained unheated solvent-extracted cottonseed kernel and hulls, while the remaining two diets contained similar material but which had been heat-treated by autoclaving at 110° for 120 min. In Expt 2, twelve rats and twelve pigs were fed on four semi-purified diets containing commercial cottonseed meal (CSM) as the only protein source. Cr2O3, was added to all diets as an indigestible marker. For each pair of diets in both experiments, PEG was either included or excluded. The effect of CT was assessed by comparing control animals (-PEG; CT acting) with PEG supplemented animals (+ PEG; CT inactivated). Ileal contents from the terminal 150 and 450 mm of ileum were collected at slaughter, 7 h from the start of feeding, for the rats and pigs respectively. Apparent ileal amino acid digestibility for rats fed on the diet containing cottonseed kernel and hulls was significantly depressed by the heat treatment, particularly for lysine and threonine. On average, apparent ileal amino acid digestibility in the diets without PEG was decreased from 0·80 to 0·70 by heat treatment. Dietary cottonseed CT depressed apparent ileal protein digestibility in the pig and in the rat. The addition of PEG to the diets significantly increased the apparent ileal digestibility of N and some amino acids for the pigs and the rats. The mean increase in apparent ileal digestibility due to PEG addition for the fourteen amino acids was 2 percentage units in both species fed on the commercial CSM diets, and 2 or 4 percentage units in rats fed on the unheated or the heated cottonseed kernel and hull diets respectively. The effect of PEG was similar in the heated and unheated cottonseed kernel and hulls for most amino acids, but apparent ileal digestibilities of threonine, tyrosine and lysine were increased more by PEG in heated than in unheated CSM. Apparent ileal N digestibility was lower in the pig than in the rat. For several of the amino acids there were significant animal species differences in apparent ileal digestibility. Studies into the effects of cottonseed CT should be carried out in the target animal species. The commercial CSM had a low apparent ileal amino acid digestibility overall, particularly for the essential amino acids lysine and threonine. It was concluded that effects of heating did not eliminate the reversible reactivity of cottonseed CT on amino acid digestion in rats and pigs but rather appeared to increase it for threoniw, tyrosine and lysine in Expt 1, causing large reductions in apparent ileal digestibility of these amino acids.

The effect of fibre level in protein-free diets on the recovery and amino acid composition of endogenous protein collected from the ileum in pigs was studied. Although the Heal nitrogen excretion increased slightly with dietary fibre level, no significant difference was observed on the amino acid recovery between diets containing 30, 60,90 or 120 g wood cellulose per kg.

Water balance, electrolyte and acid-base status of a group of five South African Mutton Merino wethers given chopped lucerne hay ad libitum was determined before and after ileorectal anastomosis. In general, the sheep recovered rapidly from surgery, resuming, within 10 days, a level of dry matter intake only slightly less than pre-operative levels (1323 (s.e. 147) and 1419 (s.e. 196) g/day respectively, P > 0·05). A new water balance was established at about the same time, when sheep with an ileorectal anastomosis drank about 2500 ml/day more water, while losing about 2400 ml/day more water in the faeces and about 365 ml/day less water in the urine than did the control sheep. Most of the electrolyte and acid-base parameters tested were not significantly affected by the anastomosis but the concentrations of oxygen and potassium in the blood of sheep with an anastomosis were significantly lower (P < 0·001), while those of chloride (P < 0·001) and bicarbonate (P < 0·05) were significantly higher, than before surgery. These observations, together with normal blood pH values, suggest that bypassing the large intestine caused a compensated metabolic alkalosis.

Energy balance was determined on the five sheep with an ileorectal anastomosis as well as five control animals in an indirect, open-circuit calorimeter. Despite a significantly lower (P < 0·05) loss of nitrogen and energy via the urine (due to a lower output of urine) in sheep with an ileorectal anastomosis, total heat loss, whether absolute or relative to energy intake, did not differ from control values and therefore indicated that basal metabolic rate was not significantly affected.

From post-mortem examinations, anastomoses appear to have induced a marked atrophy of the bypassed caecum and colon, whilst the mass of the large intestine was significantly less than in control animals, despite the significant enlargement of the rectum. On the other hand, the small intestine appeared unaffected, either macroscopically or histologically.

The most significant physiological changes that occurred on bypassing the large intestine were related to loss of water reabsorbtion. However, good adaptation to the increased turn-over of water was evident, providing free access to water and a salt lick was allowed.