Herbicide carrier water hardness and pH can be variable depending on the source and geographic location. Herbicide efficacy can be affected by the pH and hardness of water used for spray solution. Field and greenhouse studies were conducted to evaluate the effect of carrier water pH and hardness on premixed dicamba and glyphosate efficacy. Treatments were combinations of water pH at 4, 6.5, or 9; and water hardness at 0 (deionized water), 400, or 800 mg L−1 of CaCO3 equivalent. In the field study, dicamba and glyphosate were applied at 0.55 and 1.11 kg ae ha−1, respectively, and half of these rates were applied in the greenhouse study. There was no interaction between carrier water pH and hardness on dicamba and glyphosate efficacy; however, the main effects of carrier water pH and hardness were significant. Herbicide efficacy was reduced with carrier water at pH 9 compared with pH 4. In the field study, common lambsquarters, common ragweed, horseweed, or Palmer amaranth control was improved 6% or more at carrier water at pH 4 compared with pH 9. Similar results were observed with water pH for giant ragweed, Palmer amaranth, or pitted morningglory control in the greenhouse study. Carrier water hardness at 400 or 800 mg L−1 reduced common ragweed, giant ragweed, or horseweed control compared with 0 mg L−1. Similarly, common lambsquarters, Palmer amaranth, or pitted morningglory control was reduced at least 10% with carrier water hardness at 800 mg L−1 compared with 0 mg L−1. These results indicate carrier water at acidic pH and of no hardness is critical for dicamba and glyphosate application, and spray solution needs to be amended appropriately for an optimum efficacy.

Evolution of glyphosate-resistant (GR) weeds, such as horseweed, presents major challenges in no-till soybean production systems. Effective GR horseweed control with preplant burndown applications is necessary to prevent potential soybean yield losses due to competition and to manage the soil weed seedbank. Halauxifen-methyl is a new synthetic auxin herbicide for broadleaf weed control in preplant burndown applications for soybean and other crops at low use rates (5 g ae ha–1). Experiments were conducted to evaluate the efficacy of herbicide treatments containing halauxifen-methyl for control of GR horseweed in comparison to existing herbicide treatments utilized in no-till GR soybean systems. Glyphosate alone controlled horseweed 33%. Herbicide treatments that included halauxifen-methyl, dicamba, or saflufenacil in combination with glyphosate controlled horseweed 87% to 96%, 89%, and 93%, respectively, 35 d after burndown application (DAB). Horseweed control, horseweed density reduction, and ground cover reduction by halauxifen-methyl plus glyphosate was similar to dicamba plus glyphosate. Horseweed control was greater for halauxifen-methyl plus glyphosate than for 2,4-D plus glyphosate. Cloransulam, cloransulam plus flumioxazin, and cloransulam plus sulfentrazone added to halauxifen-methyl plus glyphosate increased horseweed control and reduced horseweed density. No herbicide injury or soybean yield reduction was observed for treatments containing halauxifen-methyl.

Synthetic auxin herbicides such as 2,4-D and dicamba are often utilized to control broadleaf weeds in preplant burndown applications to soybean. Halauxifen-methyl is a new synthetic auxin herbicide for broadleaf weed control in preplant burndown applications to corn, cotton, and soybean at low use rates (5 g ae ha–1). Field experiments were conducted to evaluate efficacy and weed control spectrum of halauxifen-methyl applied alone and in mixtures with 2,4-D (560 g ae ha–1), dicamba (280 g ae ha–1), and glyphosate (560 g ae ha–1). Glyphosate-resistant (GR) horseweed was controlled with halauxifen-methyl applied alone (90% control) and in mixtures (87% to 97% control) 35 d after treatment (DAT). Common ragweed was controlled 93% with halauxifen-methyl applied alone and 91% to 97% in mixtures 35 DAT. Halauxifen-methyl applied alone resulted in poor giant ragweed control 21 DAT (73% control); however, mixtures of halauxifen-methyl with 2,4-D, dicamba, or glyphosate controlled giant ragweed (86% to 98% control). Halauxifen-methyl alone resulted in poor redroot pigweed control (62% control) 21 DAT; however, mixtures of halauxifen-methyl with dicamba, 2,4-D, or glyphosate controlled redroot pigweed (89% to 98% control). Halauxifen-methyl controls GR horseweed and common ragweed applied alone and in mixtures with other synthetic auxin herbicides and glyphosate. Furthermore, mixing 2,4-D or dicamba with halauxifen-methyl can increase the weed control spectrum in preplant burndown applications.

The introduction of 2,4-D–resistant soybean will provide an additional POST herbicide site of action for control of herbicide-resistant broadleaf weeds. The introduction of this technology also brings concern of off-site movement of 2,4-D onto susceptible crops such as sensitive soybean and tomato. The 2,4-D formulation approved for use in 2,4-D–resistant soybean restricts application of the herbicide to nozzles that produce very coarse to ultra-coarse droplet spectrums. The use of larger droplet spectrums for broadcast applications can reduce herbicide deposition onto target weeds and thus influence herbicide efficacy. Field experiments were conducted to evaluate the influence of nozzle design on herbicide deposition onto target plants and the resulting efficacy of a POST application of 280 g ha−1 glyphosate plus 280 g ha−1 2,4-D. The TTI11004 nozzle produced an ultra-coarse droplet spectrum and reduced coverage and deposition density on spray cards as compared with the XR11004 and TT11004 nozzles that produced medium droplet spectrums. The AIXR11004 nozzle also reduced deposition density on spray cards but did not reduce coverage. Herbicide solution deposition onto glyphosate-resistant Palmer amaranth, tall waterhemp, giant ragweed, and horseweed ranged from 0.28 to 0.72 µl cm−2 and was not influenced by nozzle design. Herbicide efficacy was reduced by the TTI11004 nozzle on Palmer amaranth and horseweed compared with the AIXR11004, TT11004, and XR11004 nozzles when applications were made to either high densities of plants or plants exceeding the labeled height. The use of the AIXR11004 and TTI11004 nozzles that are listed as approved nozzles for glyphosate plus 2,4-D applications on 2,4-D–resistant soybean did not reduce herbicide deposition onto four of the most troublesome broadleaves and did not reduce herbicide efficacy when applied in conjunction with lower weed densities and smaller weeds.

Dicamba-resistant soybean technology provides an additional site of action for POST control of herbicide-resistant broadleaf weeds in soybean but also raises concern of off-site movement and damage to sensitive crops in adjacent fields. Dicamba formulations approved for use on dicamba-resistant soybean require applicators to use nozzles producing large droplets to reduce the risk of spray-particle drift. The use of nozzles with relatively larger droplet spectra can reduce herbicide deposition on target weeds, especially if a filtering effect from the crop canopy occurs. Experiments were conducted to evaluate the influence of broadcast nozzle design on the deposition and efficacy of 280 g ha−1 glyphosate plus 140 g ha−1 dicamba applied POST to four herbicide-resistant weed species. The TTI11004 nozzle, the original nozzle labeled for dicamba applications on dicamba-resistant soybean, reduced deposition coverage and density on spray cards compared with the TT11004 and XR11004 nozzle. The AIXR11004 nozzle produces a very coarse droplet spectrum and did not reduce coverage on spray cards, though it did reduce deposition density. Herbicide solution deposition onto Palmer amaranth, tall waterhemp, giant ragweed, and horseweed ranged from 0.41 to 0.52, 0.55 to 0.87, 0.49 to 0.58, and 0.38 to 0.41 µl cm−2, respectively. Nozzle design and droplet spectrum did not influence the deposition of herbicide solution onto the target weed, as all nozzles were equivalent for all species and site-years. Herbicide efficacy was not influenced by nozzle design, as weed control and plant height reduction were similar for all species. The results of this experiment show that the use of the TTI11004 nozzle for dicamba applications to dicamba-resistant soybean will provide acceptable herbicide deposition and efficacy when applied under the label requirements of weed height and carrier volume.

Populations of horseweed and Virginia pepperweed resistant to the herbicide paraquat were found in several fruit orchards in Essex County, Ontario, Canada, where paraquat had been applied four to five times per year for at least 10 yr. Dose–response experiments were conducted in the greenhouse for each species, using 2-to 4-mo-old plants from populations that had received repeated exposure to paraquat and those that had not. The paraquat dose that reduced shoot dry weight by 50% (ED 50) was estimated for each population. Resistant populations of horseweed had ED 50 values 25 to 35 times higher than susceptible populations. A resistant population of Virginia pepperweed had an ED 50 10 times higher than susceptible populations.

Spray water quality is an important consideration for optimizing herbicide efficacy. Hard water cations in the carrier water can reduce herbicide performance. Greenhouse studies were conducted to evaluate the influence of hard water cations and the use of ammonium sulfate (AMS) on the efficacy of 2,4-D choline and premixed 2,4-D choline plus glyphosate for giant ragweed, horseweed, and Palmer amaranth control. Carrier water hardness was established at 0, 200, 400, 600, 800, or 1,000 mg L−1 using CaCl2 and MgSO4, and each hardness level consisted of without or with AMS at 10.2 g L−1. One-third of the proposed use rates of 2,4-D choline at 280 g ae ha−1 and 2,4-D choline plus glyphosate at 266 plus 283 g ae ha−1, respectively, were applied in the study. An increase in carrier water hardness showed a linear trend for reducing 2,4-D choline and 2,4-D choline plus glyphosate efficacy on all weed species evaluated in both studies. The increase in water hardness level reduced giant ragweed control with 2,4-D choline and the premix formulation of 2,4-D choline plus glyphosate to a greater extent without AMS than it did with AMS in the spray solution. Increases in water hardness from 0 to 1,000 mg L−1 reduced weed control 20% or greater with 2,4-D choline. Likewise, the efficacy of the premixed 2,4-D choline plus glyphosate was reduced 21% or greater with increased water hardness from 0 to 1,000 mg L−1. The addition of AMS improved giant ragweed, horseweed, and Palmer amaranth control ≥ 17% and ≥ 10% for 2,4-D choline and 2,4-D choline plus glyphosate application, respectively. The biomass of all weed species was reduced by ≥ 8% and ≥ 5% with 2,4-D choline and 2,4-D choline plus glyphosate application, respectively, when AMS was added to hard water.

Carrier water quality is an important consideration for herbicide efficacy. Effect of carrier water pH (4, 6.5, or 9) and coapplied Zn or Mn foliar fertilizer was evaluated on glufosinate efficacy for horseweed and Palmer amaranth control in the field. Greenhouse studies were conducted to evaluate the effect of: (1) carrier water pH, foliar fertilizer (Zn, Mn, or without fertilizer), and ammonium sulfate (AMS) (at 0 or 2.5% v/v); and (2) carrier water hardness (0 to 1,000 mg L−1) and AMS (at 0 or 2.5% v/v) on glufosinate efficacy for giant ragweed, horseweed, and Palmer amaranth control. In a 2014 field study, control, plant density reduction, and biomass reduction were at least 8% greater for horseweed and at least 14% greater for Palmer amaranth when glufosinate was applied at carrier water pH 4 compared with pH 9. Glufosinate efficacy was at least 10 and 17% greater for giant ragweed and Palmer amaranth control, respectively, with carrier water pH 4 compared with pH 9 in the greenhouse. In the greenhouse studies, coapplied Zn or Mn fertilizer had no effect on glufosinate efficacy. Increased carrier water hardness from 0 to 1,000 mg L−1 negatively influenced glufosinate efficacy and resulted in 20 and 17% lesser control and biomass reduction, respectively, on giant ragweed or Palmer amaranth. Use of AMS enhanced glufosinate efficacy on giant ragweed control in both greenhouse studies, whereas only the Palmer amaranth control was enhanced in the water hardness study. Horseweed control with glufosinate as affected by carrier water pH, hardness, or AMS remained unaffected in both greenhouse studies. Carrier water at alkaline pH or hardness > 200 mg L−1 has potential to reduce glufosinate efficacy. Therefore, carrier water free of hardness cations and at acidic condition (pH = 4 to 6.5) should be considered for optimum glufosinate efficacy.

Herbicide-resistant weeds have impacted crop production throughout the United States, but the effect they have on extension programming has not been evaluated. In June 2007, 38 extension weed specialists throughout the United States, responded to a survey on herbicide-resistant (HR) weeds and the impact they are having on extension education programming. Survey results revealed that HR weeds have had a significant impact on extension programming particularly for agronomic crops. In the last 10 yr, agronomic weed specialists' extension programming was almost twice as likely to be impacted by the presence of HR weeds as compared to horticultural programming. In the next 5 yr, agronomic extension programming is twice as likely to be altered. Of 37 weed species reported, seven genera or species of weeds represented 80% of the major HR biotypes reported. These include Amaranthus species, horseweed, Setaria species, common lambsquarters, kochia, giant ragweed, and Lolium species. Five weed species (common ragweed, common lambsquarters, horseweed, kochia, and three foxtail species) exhibited weed by mode of action (MOA) interactions when evaluated as major or minor problems. Herbicide resistance problem severity differed for weed species, herbicide MOA, and crops. The results of this survey of university extension personnel confirm that HR weeds have impacted extension programming and will continue to impact programming in the future.

Certified Crop Advisors of Arkansas and members of the Arkansas Crop Consultants Association were surveyed in Fall 2006 through direct mail to assess current weed management practices and needs in cotton from both a research and educational perspective. Consultants reported scouting 162,300 of the possible 473,700 ha of cotton grown in Arkansas. Collectively, glyphosate-resistant and enhanced glyphosate-resistant cultivars were reported grown on 98% of the cotton hectares. Ninety-five percent of the consultants believe the planting of enhanced glyphosate-resistant cultivars will increase over the next 5 yr. All consultants indicated a “moderate” to “high” level of concern with herbicide-resistant weeds in cotton, and 79% of the consultants suspect herbicide resistance in the fields they scout, predominately glyphosate-resistant horseweed. Horseweed, Palmer amaranth, and morningglories were the three most problematic weeds in cotton. A continued focus on resistant weed management was the most frequent research and educational request by consultants.

The influence of environmental factors on germination and emergence of horseweed was examined in growth chamber experiments. Germination was highest (61%) under 24/20 C day/night temperature under light. Horseweed seed germination was observed under both light (13 h photoperiod) and complete darkness (24 h), but germination under continuous darkness was only 0 to 15% compared with 0 to 61% under light. All other experiments were conducted under 24/20 C and 13-h light conditions. Germination was 19 to 36% over a pH range from 4 to 10, with a trend toward higher germination under neutral-to-alkaline conditions. Horseweed germination was > 20% at < 40 mM NaCl concentration and lowest (4%) at 160 mM NaCl. These data suggest that even at high soil salinity conditions, horseweed can germinate. Germination of horseweed decreased from 25% to 2% as osmotic potential increased from 0 (distilled water) to −0.8 MPa, indicating that germination can still occur under moderate water stress conditions. Horseweed seedling emergence was at its maximum on the soil surface, and no seedlings emerged from seeds placed at a depth of 0.5 cm or higher.

Root colonization by soil microorganisms has been shown to increase the activity of glyphosate in resistant and susceptible biotypes of giant ragweed and a susceptible common lambsquarters biotype, but not in horseweed biotypes. The objective of this study was to investigate the colonization of roots in glyphosate-resistant and -susceptible giant ragweed and horseweed biotypes, and glyphosate-tolerant and -susceptible biotypes of common lambsquarters after a sublethal glyphosate application. The three weed species were grown separately in sterile and unsterile field soil and treated with glyphosate at two sublethal rates. Soil microbes were isolated from the roots onto sterile media 3 d after the glyphosate treatment. The susceptible biotypes of giant ragweed and horseweed grown in unsterile soil were colonized by more soil microbes at the higher rate of glyphosate, compared to the resistant biotype grown in unsterile soil. Oomycetes were isolated separately on a selective media and they were also more prevalent in the roots of the susceptible biotypes of each weed species grown in the unsterile soil when glyphosate was applied at the highest rate. Therefore, the ability of these three weed species to tolerate a glyphosate application may involve differences in the susceptibility to soil microbial colonization, especially oomycetes.

The growth regulator herbicides 2,4-D and dicamba are used to control glyphosate-resistant horseweed before crops are planted. With the impending release of 2,4-D–resistant and dicamba-resistant crops, use of these growth regulator herbicides postemergence will likely increase. The objective of this study was to determine the effectiveness of various growth regulators on Indiana horseweed populations. A greenhouse dose–response study was conducted to evaluate the effectiveness of 2,4-D ester, diglycolamine salt of dicamba, and dimethylamine salt of dicamba on control of four populations of horseweed in the greenhouse. Population 66 expressed twofold levels of tolerance to 2,4-D ester and diglycolamine salt of dicamba. Population 43 expressed an enhanced level of tolerance to diglycolamine salt of dicamba but not to the other herbicides. Diglycolamine salt of dicamba provided the best overall control of populations 3 and 34. Additionally, a field study was conducted to evaluate standard use rates of 2,4-D amine, 2,4-D ester, diglycolamine salt of dicamba, and dimethylamine salt of dicamba on control of various sized glyphosate-resistant horseweed plants. Control of plants 30 cm or less in height was 90% or greater for all four herbicides. On plants greater than 30 cm tall, diglycolamine salt of dicamba provided 97% control while 2,4-D amine provided 81% control. Diglycolamine salt of dicamba provided the highest level of control of glyphosate-resistant horseweed, followed by dimethylamine salt of dicamba, 2,4-D ester and 2,4-D amine, respectively. This research demonstrates that horseweed populations respond differently to the various salts of 2,4-D and dicamba, and it will be important to determine the appropriate use rates of each salt to control glyphosate-resistant horseweed.

Water is the primary carrier for herbicide application, and carrier-water–related factors can influence herbicide performance. In a greenhouse study, premixed formulation of glyphosate plus dicamba was mixed in deionized (DI) water at 5, 18, 31, 44, or 57 C and applied immediately. In a companion study, glyphosate and dicamba formulation was mixed in DI water at temperatures of 5, 22, 39, or 56 C and sprayed after the herbicide solution was left at the respective temperatures for 0, 6, or 24 h. In both studies, glyphosate plus dicamba was applied at 0.275 plus 0.137 kg ae ha−1 (low rate), and 0.55 plus 0.275 kg ha−1 (high rate), respectively, to giant ragweed, horseweed, Palmer amaranth, and pitted morningglory. Glyphosate plus dicamba applied at a low rate with solution temperature of 31 C provided 14% and 26% greater control of giant ragweed and pitted morningglory, respectively, compared to application at solution temperature of 5 C. At both rates of glyphosate and dicamba formulation, giant ragweed and pitted morningglory control was 15% or greater at solution temperature of 44 C compared to 5 C. Weed control was not affected with premixture of glyphosate and dicamba applied ≤ 24 h after mixing herbicide. When considering solution temperature, glyphosate and dicamba applied at low rate provided 13 and 6% greater control of Palmer amaranth and pitted morningglory, respectively, with solution temperature of 22 C compared to 5 C. Similarly, giant ragweed control was 8% greater with solution temperature of 39 C compared to 5 C. Glyphosate and dicamba applied at high rate provided 8% greater control of giant ragweed at solution temperature of 22 or 39 C compared to 5 C. Therefore, activity of premixed glyphosate and dicamba could be reduced with spray solution at lower temperature; however, the result is dependent on weed species.

Two rapid, nondestructive assays were developed and tested for their potential in differentiating glyphosate-resistant from glyphosate-susceptible biotypes of horseweed. In one assay, leaves of glyphosate-resistant and -susceptible corn, cotton, and soybean plants as well as glyphosate-resistant and -susceptible horseweed plants were dipped in solutions of 0, 300, 600, and 1200 mg ae L−1 glyphosate for 3 d and subsequent injury was evaluated. In the second assay, plant sensitivity to glyphosate was evaluated in vivo by incubating excised leaf disc tissue from the same plants used in the first assay in 0.7, 1.3, 2.6, 5.3, 10.6, 21.1, 42.3, and 84.5 mg ae L−1 glyphosate solutions for 16 h and measuring shikimate levels with a spectrophotometer. The leaf-dip assay differentiated between glyphosate-resistant and -susceptible crops and horseweed biotypes. The 600 mg L−1 rate of glyphosate was more consistent in differentiating resistant and susceptible plants compared with the 300 and 1,200 mg L−1 rates. The in vivo assay detected significant differences between susceptible and glyphosate-resistant plants of all species. Shikimate accumulated in a glyphosate dose-dependent manner in leaf discs from susceptible crops, but shikimate did not accumulate in leaf discs from resistant crops and levels were similar to nontreated leaf discs. Shikimate accumulated at high (≥ 21.1 mg ae L−1) concentrations of glyphosate in leaf discs from all horseweed biotypes. Shikimate accumulated at low glyphosate concentrations (≤ 10.6 mg L−1) in leaf discs from susceptible horseweed biotypes but not in resistant biotypes. Both assays were able to differentiate resistant from susceptible biotypes of horseweed and might have utility for screening other weed populations for resistance to glyphosate.

Diclosulam is registered for residual and postemergence control of several broadleaf weeds and suppression of annual sedges in peanut in the southeastern United States. Many producers apply herbicides and other pesticides simultaneously to increase the spectrum of pest control or to increase efficiency of operations. However, compatibility of coapplication of pesticides is a concern. Field trials were conducted to evaluate the compatibility of diclosulam with other herbicides and fungicides. Horseweed control by diclosulam in combination with glyphosate, sulfosate, or paraquat was compared to combinations of these herbicides with flumioxazin, tribenuron plus thifensulfuron, or 2,4-D. All treatments that contained diclosulam controlled horseweed at least 86%. Broadleaf signalgrass control by clethodim and sethoxydim was not affected by diclosulam; however, large crabgrass control was reduced when graminicides were coapplied with diclosulam. Common ragweed control was reduced when diclosulam was applied with chlorothalonil and pyraclostrobin but not by azoxystrobin or tebuconazole.

Managing glyphosate-resistant (GR) horseweed in no-till cotton continues to be a serious challenge for midsouthern producers. Field studies were conducted in 2008 and 2009 to evaluate spring burndown applications of saflufenacil on GR horseweed prior to planting cotton. Saflufenacil controlled GR horseweed at least 94% up to 7 d before planting (DBP) without causing significant cotton injury. Saflufenacil applied at 7 or 14 DBP controlled GR horseweed while still providing residual control until planting. Moreover, saflufenacil, on silt loam soil evaluated in this study, showed no more injury than dicamba applied 7 or more DBP. Results indicated that saflufenacil is an option in cotton for controlling GR horseweed much closer to cotton planting than 42 DBP (current saflufenacil label). At 25 g ha−1, which is the standard labeled rate in cotton, saflufenacil provided > 90% control of GR horseweed. Saflufenacil as a GR horseweed burndown, could replace the current dicamba standard every other year to reduce the probability of horseweed developing resistance to dicamba or salflufenacil.

The mechanism of glyphosate resistance in horseweed was investigated. Eleven biotypes of putative sensitive (S) and resistant (R) horseweed were obtained from regions across the United States and examined for foliar retention, absorption, translocation, and metabolism of glyphosate. Initial studies used spray application of 14C-glyphosate to simulate field application. When S and R biotypes were compared in the absence of toxicity at a sublethal dose, we observed comparable retention and absorption but reduced root translocation in the R biotypes. S and R biotypes from Delaware were further examined at field use rates and results confirmed similar retention and absorption but reduced root translocation in the R biotypes. Application of 14C-glyphosate to a single leaf demonstrated reduced export out of the treated leaf and lower glyphosate import into other leaves, the roots, and the crown in R relative to S biotypes. Examination of the treated leaf by autoradiography showed that glyphosate loading into the apoplast and phloem was delayed and reduced in the R biotype. Our results consistently showed a strong correlation between impaired glyphosate translocation and resistance. Tissues from both S and R biotypes showed elevated levels of shikimate suggesting that 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) remained sensitive to glyphosate. Analysis of tissue shikimate levels demonstrated reduced efficiency in EPSPS inhibition in the R biotypes. Our results suggest that resistance is likely due to altered cellular distribution that impaired phloem loading and plastidic import of glyphosate resulting in reduced overall translocation as well as inhibition of EPSPS.

Dicamba or 2,4-D will be used POST for the control of weeds in soybean when dicamba- or 2,4-D-resistant soybean are commercialized. The active ingredients of both herbicides are weak acids in solution and may bind to cations present from hard water used as herbicide carrier or from foliar fertilizers added to spray solutions. The objectives of this research were (1) to determine if the efficacy of dicamba or 2,4-D are influenced by divalent cations, namely calcium (Ca), magnesium (Mg), manganese (Mn), and zinc (Zn), in the spray solution, and (2) to determine if adding ammonium sulfate (AMS) to the spray solution can overcome antagonism. The factorial study included five cation solutions (deionized water [dH2O], Ca at 590 mg L−1, Mg at 630 mg L−1, Mn at 4.97 L ha−1, and Zn at 2.33 L ha−1), two herbicide treatments (dicamba or 2,4-D), and two water conditioner treatments (without or with AMS at 20.37 g L−1). Treatments were applied to common lambsquarters, horseweed, and redroot pigweed. Control of horseweed and redroot pigweed increased when AMS was added to the 2,4-D treatments, irrespective of cation solution. Control of common lambsquarters was increased when AMS was added to 2,4-D for only the Ca and Mn cation solution. In contrast to the results obtained with 2,4-D, control of horseweed with dicamba was not influenced by cation solution. Tank-mixing AMS with dicamba increased control of both redroot pigweed and common lambsquarters in the dH2O, Mg, and Mn solutions.

Since the first identification of glyphosate resistance in horseweed in California in 2005, the glyphosate-sensitive (GS) biotype has become rare, whereas the glyphosate-resistant (GR) biotype has become dominant in the eastern San Joaquin Valley (SJV). This is an area exposed to regular usage of glyphosate and to high levels of ambient ground-level ozone (O3). A previous study showed that SJV biotypes of GR are more robust than GS in the absence of ozone. This advantage was reduced, though not eliminated, at elevated O3. This suggests that the rapid evolution of resistance to glyphosate was not linked to evolution of resistance to O3. In this study, we contrasted these responses to O3 in the presence of concurrent glyphosate pressure. The GR and GS biotypes differed in growth and injury, reflecting their known differential sensitivities to glyphosate, but responded similarly to O3 with no O3 × biotype interaction. Ozone imposed an unexpected, but ecologically important, impact that enhanced the performance advantage of GR over GS. In the presence of the combination of glyphosate and O3 the biomass of GR was reduced to low but viable levels, whereas the biomass of GS was reduced to nonviable levels that effectively removed it from the population. These data do not support a genetic linkage between resistance to glyphosate and to O3, but suggest that air pollution may have accelerated the fixation of glyphosate-resistance alleles in California horseweed populations.