Native Cirsium species play an important role in landscapes across North America. Hadroplontus litura (F.) (formerly Ceutorhynchus litura), the stem-mining weevil and biological control agent of Canada thistle [Cirsium arvense (L.) Scop.] can complete its life cycle on five Cirsium species native to the upper Midwest. Although these five Cirsium species are within the fundamental host range of H. litura, as determined by host-range tests, we wanted to explore whether phenological differences among Cirsium species help define the field ecological host range of H. litura. The objective of this study was to determine the phenology of Cirsium species native to the upper Midwest in relation to C. arvense and H. litura. Our goal was to explore whether shoots of native Cirsium species could escape H. litura shoot oviposition in spring due to delayed shoot emergence relative to C. arvense. Soil cumulative growing degree days (GDD) were a superior predictor of shoot emergence for perennial Cirsium species or initiation of leaves in biennial Cirsium species, with a 2.4 times larger effect on time to emergence relative to air GDD. All native Cirsium species initiated new leaves or shoots before C. arvense shoot emergence, even when native Cirsium species growth was delayed in the spring. In turn, C. arvense shoots emerged approximately 1 to 3 wk before female H. litura began to lay eggs. As such, all native Cirsium plants had shoots available for H. litura oviposition. There was no phenological separation between native Cirsium and C. arvense shoot emergence or initiation that would render native Cirsium species safe from H. litura attack. Based on the phenology of shoot emergence or initiation in the spring, all tested Cirsium species native to the upper Midwest would be within the ecological host range of H. litura.

We examined effects of supplementary nitrogen on young Canada thistle stands in Alberta, Canada. Urea applied at 70 or 100 kg N ha–1 increased shoot population densities, mostly through increased root growth in the top 20 cm of soil rather than through released root bud dormancy. Nitrogen fertilization, therefore, may increase the severity of a Canada thistle infestation. In 1-yr-old stands, the number of emerged and unemerged root buds/m of root was higher near the soil surface in fertilized plots than in unfertilized plots, and no underground shoots were detected at the time of sampling. In 2-yr-old stands, more unemerged root buds/m of root were found at depths below 20 cm in fertilized plots than in unfertilized plots, and twice as many underground shoots/m of root occurred between 20 and 40 cm in fertilized plots as in unfertilized plots. However, no effect of nitrogen on the number of emerged root buds was detected. Nitrogen treatment increased shoot production only for those replanted root fragments from deeper than 60 cm in 1-yr-old stands and deeper than 160 cm in 2-yr-old stands.

Under field conditions, more photoassimilate moved to roots of Canada thistle at the bolt than at the bud, flower, or postflower stages. Similarly, greater photoassimilate accumulated in roots of Canada thistle in the greenhouse at the rosette and bolt than at the flower bud stage. Growth chamber experiments indicated that environmental conditions typical of fall, and possibly early spring, favored photoassimilate movement to the root and superseded growth stage control of assimilate partitioning. Allocation of assimilate within the root was strongly influenced by growth stage, with most assimilate being utilized for growth at the rosette stage and for fructan reserves in bolt and flower bud stages.

Early detection of an invading nonindigenous plant species (NIS) may be critical for efficient and effective management. Adaptive survey sampling methods may provide unbiased sampling for best estimates of distribution of rare and spatially clustered populations of plants in the early stages of invasion. However, there are few examples of these methods being used for nonnative plant surveys in which travelling distances away from an initial or source patch, or away from a road or trail, can be time consuming due to the topography and vegetation. Nor is there guidance as to which of the many adaptive methods would be most appropriate as a basis for invasive plant mapping and subsequent management. Here we used an empirical complete census of four invader species in early to middle stages of invasion in a management area to assess the effectiveness and efficiency of three nonadaptive methods, four adaptive cluster methods, and four adaptive web sampling methods that all originated from transects. The adaptive methods generally sampled more NIS-occupied cells and patches than standard transect approaches. Sampling along roads only was time-efficient and effective, but only for species with restricted distribution along the roads. When populations were more patchy and dispersed over the landscape the adaptive cluster starting at the road generally proved to be the most time-efficient and effective NIS detection method.

Invasive species such as Canada thistle pose a significant threat to ecosystems. The risk of introducing invasive species has increased with human activities, and the effects of such events have economic and aesthetic impacts. Native to Europe, Canada thistle is now established throughout temperate North America. Although there is documentation of early occurrences to North America, little is known on how it has become established in diverse habitats or how it continues to spread. We examined genetic diversity within and among nearly 1,700 Canada thistle individuals from 85 North American locations with the use of seven microsatellite markers in order to address these questions. PAUP and STRUCTURE programs were used to assess genetic diversity and relationships within and between populations. Populations exhibited greater within-population diversity (> 60%) than expected for a reported clonally reproducing species. Total diversity of sampled locations in North America (0.183) was less than previously reported for European locations (0.715), but the greater mean difference between North American populations (0.264 relative to 0.246 from England) suggests strong founder effects or restriction of gene flow influencing individual populations. Furthermore, analyses identified numerous instances where individuals from geographically distant regions clustered together, indicating long-distance translocation of propogules. However, isolation by distance analysis showed significant correlation between location and population genetic distances (r = 0.1917, P = 0.006). Within populations, nearly 92% of individuals sampled harbored unique multilocus genotypes, strongly suggesting that sexual reproduction is common. Within populations, analysis of genetic structure indicated significant admixture of genotypes throughout the invasive range in North America. The recurrent distribution of seed throughout North America has led to a highly diverse gene pool and increased the adaptive success Canada thistle to a wide variety of habitats. Future technologies developed for control of Canada thistle should consider this diversity.

Three strategies for controlling Cirsium arvense including (i) repeated stubble tillage with subsequent forage crop cultivation, (ii) repeated mowing of a ryegrass–clover ley and (iii) forage crop cultivation following a ryegrass–clover ley ploughed in May/June were investigated in field experiments over 3 years at the Experimental Farm for Organic Agriculture ‘Wiesengut’ in North-Rhine Westphalia, Germany. The development of C. arvense (shoot density, shoot size and ground cover) was regularly assessed on fixed standardized subplots. In the medium-term (9 months), repeated stubble tillage (i) decreased shoot density and regrowth capacity of C. arvense more effectively than a mowed ryegrass–clover ley (ii and iii). However, after 22 months, strategies (i) and (ii) resulted in a similar strong reduction of C. arvense shoot density of 95 and 97%, respectively. At this time, the efficacy of strategy (iii) (89%) was not significantly different to that of strategies (i) and (ii). After 26 months, the effect of all strategies was still apparent; however, the efficacy of strategy (iii) was significantly lower than that of strategy (ii). Generally, the different strategies showed only minor differences, thus delivering options for optimal strategies of thistle control under given specific conditions of sites and cropping systems.