Pitfall-trap sampling was used to determine the activity (numbers collected as determined by locomotor activity and density) of epigaeic (soil-surface dwelling) arthropods in rice cultivated at various toposequence sites on the slope of an inland valley in Bouaké, Côte d'Ivoire, West Africa in 1994–1996. The major arthropod groups collected were ants, spiders, crickets, carabids and grasshoppers. Ants and spiders were the most active. Arthropod activity changed in response to crop growth stage with activity generally increasing towards harvest. Ant activity differed significantly between toposequence sites and was highest in the upper portion of the slope. The pattern for spider activity in relation to toposequence site varied among years. Agronomic practices that would enhance the populations of ground-dwelling predaceous arthropods are an important consideration when developing cultural practices for rice production on hillsides of inland valleys in West Africa.

Studies on the population dynamics of Aphis gossypii Glover carried out on cotton in the Tadla area over a 3-year period showed two periods of rapid resurgence: June-July and September, with the highest density reached in the latter month accompanied by considerable indirect economic loss. The resurgence of Aphis gossypii populations on cotton depends mainly on the date and the density of contamination flights. The evolution and other factors responsible for the variations in the population dynamics are discussed.

The biology, insect density-yield loss relationships, genotype reactions, and consumption and utilisation of food by the legume pod borer, Maruca vitrata (Fabricius) on pigeonpea (Cajanus cajan) and Adzuki bean (Phaseolus angularis) were studied. Post-embryonic development was completed in 20.2 to 22.6 days. Grain yield in plants infested with 2 and 4 larvae was the same as that in uninfested controls, while plants infested with 8 and 16 larvae suffered between 50–68% grain yield loss in pigeonpea and 61–71% in Adzuki bean. Food consumption by third instars was highest on ICPL 90011. This genotype had the lowest approximate digestibility (AD) and efficiency of conversion of ingested food (ECI). The significance of these results in relation to economic damage thresholds is discussed.

The life cycle and development of Zonocerus variegatus (variegated grasshopper) from southern Benin was studied at the International Institute of Tropical Agriculture (IITA) Benin Station, from March 1992 to August 1994. Hoppers of the 1st to the 5th stage were collected from uncultivated fields in the Atlantic Province and placed in cages and their development evaluated by counting the number of insects in each stage every two days until the adult stage. Mating and the number of egg pods were also recorded. The study showed that Z. variegatus has only one generation in southern Benin, running from September/October to July/August. Hatching occurs between September/ October and February/March. Zonocerus variegatus females can oviposit up to five times and the interval between ovipositions varies from 14 to 32 days. The variegated grasshopper has an egg diapause and egg hatching is not synchronised. These observations explain the almost perennial presence of the variegated grasshopper in southern Benin.

Preferences of Maruca vitrata (Fabricius) (Lepidoptera: Pyralidae) larvae to flowers, flower buds and pods of three different varieties of cowpea (IT 82E-666, IT 82D-716 and Botokpoyi) were studied in the laboratory using a four-branched olfactometer. For each plant part, fourth instars of M. vitrata were individually exposed to a set of three odours obtained from the cowpea varieties with pure air serving as the control. Movements of the larvae in the exposure chamber were followed at two levels of choice. Results from the first level of choice showed that the attractiveness of a particular plant part to M. vitrata larvae was not significantly influenced by variety. At the second level of choice a weakly significant preference was found for flowers (P = 0.1218) compared to pods (P = 0.7530) and flower buds (P = 0.6172). In all cases, no significant difference was found between the control (pure air) and the varieties.

The volatility and mosquito repellency of the essential oil of Hemizygia welwitschii Rolfe (Laminaceae) and its prototype pharmaceutical formulations were examined and compared with those of a commercial mosquito repellent. At ambient temperature (28°C), neat Hemizygia oil had a volatility of 4.47±1.02 mg/cm2/ h for 15 hours, after which the rate declined. The oil evaporated at 200 μg/ cm2/ h from 10% w/w liquid paraffin solutions, and at 60 μg/ cm2/ h from soft paraffin during the first 20 hours, after which the decline in evaporation rate was generally bi-phasic. The oil demonstrated concentration-dependent repellency against the mosquito Aedes aegypti. Its liquid paraffin solutions (5% to 15% concentration) prevented up to 50% of starved mosquitoes from feeding on a blood meal for 6 hours. Lower concentrations were less effective and inhibited feeding for only 1 hour. A 15% w/w oil-in-water emulsion formulation of Hemizygia oil produced 55% feeding deterrence in the mosquitoes for 2 hours. Neither the solution nor the emulsion formulations of the oil provoked any adverse reaction on human skin.

To study the effect of temperature on the development and predatory behaviour of Culex (Lutzia) tigripes, immature stages of the predatory mosquito Culex (Lutzia) tigripes were reared at temperatures ranging from 12–37°C and provided Culex quinquefasciatus larvae as prey. Larval developmental duration decreased with an increase in rearing temperature until an optimum temperature of 30°C was reached. Above this temperature, the immatures developed slower. In contrast, pupal developmental duration decreased at temperatures above 30°C, being shortest at 32°C. The lower and upper threshold temperatures for larval development were around 12°C and 34°C respectively. Fourth instars were the most voracious and had the longest developmental time. The daily rate of predation increased with temperature, with more prey larvae being consumed at 30°C. Although they consumed more prey, Cx. tigripes larvae and pupae were smaller at high temperature. The increase in prey consumption during the entire larval developmental period at 20°C was attributed to a longer larval duration at this temperature. Developmental period and growth of male and female larvae reared at a particular temperature were not significantly different. Depending on temperature, each larva consumed between 160 and 229 Cx. quinquefasciatus larvae during its development. The implications of these findings to the possible use of Cx. tigripes for mosquito control are discussed.

A study was conducted to elucidate the effects of protein fractions isolated from maize (Zea mays L.) seeds on the development and survival of the Mexican bean weevil, Zabrotes subfasciatus (Boh.) (Coleoptera: Bruchidae). Maize seeds were fractionated into albumins, globulins, prolamins, glutelins and a residue and the bruchids reared on artificial seeds composed of susceptible background bean flour enriched with the different protein fractions. Survival (as indicated by LD50) and development (as indicated by WD50) of 20-day-old larvae of the bruchid were negatively affected by most of the fractions. The albumins and globulins were the most detrimental to Z. subfasciatus, significantly affecting both its survival and development.

Changes in the pattern of haemolymph polypeptides were studied to map the innate immune response of the malaria vector Anopheles stephensi (Diptera: Culicidae) to infection by the parasite Plasmodium yoelii yoelii. In addition, changes in the ontogenetic pattern due to glucose feeding, age, sex and gonotrophic cycle-related proteins were analysed. Six polypeptides were induced and 22 polypeptides disappeared due to glucose feeding during various stages of the mosquito's adult life. Five polypeptide patterns (91, 100, 108, 133 & 145 KDa) were found exclusively in female haemolymph. The changes in these polypeptide patterns have been correlated with sexual dimorphism in their feeding behaviour.

In total, 18 polypeptides were induced in the haemolymph of parasitised mosquitoes during different stages of development. Most of the polypeptides were induced in the early stages of infection, i.e. immediately after a bloodmeal. One polypeptide (25 KDa) was induced in all the stages. The expression of two polypeptides (32.5 and 70 KDa) on day 9 and one polypeptide (42 KDa) on day 15 was also enhanced following parasitism. The different induced proteins may help mosquitoes of different ages cope with parasite infection. The parasite evidently elicits immune responses in multiple tissues of the mosquito, including two epithelia which the parasite must traverse to complete its development. The mechanism of these responses and their significance in malaria transmission are discussed.

The effect of potassium application to maize plants on the development, survival and fecundity as well as larval and pupal weight of Sesamia calamistis and Eldana saccharina were studied in the laboratory. Six different potassium treatments corresponding to 0, 30, 60, 60, 90, 120 and 150 kg K2O/ha were used. Potassium had no influence on larval and pupal developmental time or longevity of adult S. calamistis. The highest larval mortality was observed at 0 and at 150 kg K2O/ha. Increased doses (> 60 kg K2O/ha.) tended to reduce pupal survival. The lowest pupal weight was obtained at 150 kg/ha whereas fecundity was negatively related with potassium. The highest values of intrinsic rate of increase (rm) and net reproductive rate (Ro) were obtained with 60 kg K2O/ha and lowest with 150 kg K2O/ha. For E. saccharina, potassium had no significant effect on larval and pupal developmental time, larval survival, and pupal weight. The highest larval weight, pupal survival and fecundity as well as rm and Ro values were observed at 90 kg K2O/ha.

The production of the nucleopolyhedrovirus (NPV) of the tobacco cutworm, Spodoptera litura was studied to determine the optimum conditions for in vivo production using homologous host larvae reared on artificial diet. We compared NPV production in 7, 8, 9 and 10-day-old larvae and found that 9-day-old larvae gave the highest virus yield. The optimum dose was determined by infecting 9-day old larvae (weighing 125–155 mg) with a range of doses. The maximum NPV production achieved was 3.91 × 109 polyhedral inclusion bodies (PIBs) per larva using an inoculum of 4.8 × 106 PIBs. A 7-day incubation period was found to be optimum for NPV production. Moribund larvae were found to be more suitable for handling and for the mass production of virus than dead ones.

The effectiveness of aqueous neem leaf and seed extracts for the control of the diamondback moth (DBM), Plutella xylostella (Linnaeus) (Lepidoptera: Plutellidae) on cabbage was evaluated in field tests at Chokwe, Mozambique and compared with parallel dimethoate + cypermethrin treatments. All the neem treatments recorded significantly higher yield of marketable heads of cabbage and significantly better control of diamondback moth than the commonly used mixture of pyrethroids or the untreated control. The neem seed extracts provided the best control of DBM. The possibility of using neem extracts as part of an integrated pest management system for DBM control in smallholder farms is discussed.