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Optimisation of Spodoptera litura Fab. Nucleopolyhedrovirus Production in Homologous Host Larvae

Published online by Cambridge University Press:  19 September 2011

Md. Monobrullah  and
Masao Nagata
Md. Monobrullah
Affiliation:
Department of Agricultural and Environmental Biology, Faculty of Agriculture, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113–8657, Japan
Masao Nagata
Affiliation:
Department of Agricultural and Environmental Biology, Faculty of Agriculture, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113–8657, Japan
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Abstract

The production of the nucleopolyhedrovirus (NPV) of the tobacco cutworm, Spodoptera litura was studied to determine the optimum conditions for in vivo production using homologous host larvae reared on artificial diet. We compared NPV production in 7, 8, 9 and 10-day-old larvae and found that 9-day-old larvae gave the highest virus yield. The optimum dose was determined by infecting 9-day old larvae (weighing 125–155 mg) with a range of doses. The maximum NPV production achieved was 3.91 × 109 polyhedral inclusion bodies (PIBs) per larva using an inoculum of 4.8 × 106 PIBs. A 7-day incubation period was found to be optimum for NPV production. Moribund larvae were found to be more suitable for handling and for the mass production of virus than dead ones.

Résumé

La production du virus à polyhédrose nucléaire (VPN) chez le ver rongeur du tabac, Spodoptera litura, a été étudiée afin de déterminer les conditions optimales de production du virus, in vivo, par utilisation de larves hôtes homologues élevées sur un aliment artifficiel. Nous avons comparé la production du virus sur des larves âgées de 7, 8, 9 et 10 jours, et nous avons trouvé que les larves âgées de 9 jours donnaient plus de virus à polyhédrose nucléaire. Nous avons ensuite déterminé la dose optimale par soumission des larves âgées de 9 jours (avec un poids de 125–155 mg), à une série de doses. Le maximum enregistré de production du virus à polyhédrose nucléaire était de 3,91 × 109 de capsules polyhédriques par larve, partant d'une inoculation initiale de 4,8 106 capsules. Pour la productkwtdu virus, une période d'inoculation de 7 jours s'est montrée optimale. Nous avons trouvé qu'en comparaison des larves déjà mortes, les larves moribondes étaient les plus appropriées pour le mentien et la production en masse du virus.

Keywords

Spodoptera lituranucleopolyhedrovirusin vivo productionbiopesticides optimisationSpodoptera lituravirus à polyhédrose nucléaireproduction in vivooptimisation de biopesticides
Type
Research Articles
Information
International Journal of Tropical Insect Science , Volume 20 , Issue 2 , June 2000 , pp. 157 - 165
Copyright
Copyright © ICIPE 2000

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