Field studies were conducted near Oxnard, CA, to assess propagule survivability of two annual and two perennial weed species in 7.6 m3 static piles of fresh or aged yard waste mulch over a 56 d period. Mulch temperatures > 60 C generated at depths > 30 cm in fresh mulch caused all weed propagule mortality, whereas sublethal temperatures at shallower depths in fresh, and at all depths in aged piles allowed propagule survival. A controlled environment laboratory experiment suggested ≥ 1 d exposure provides 100% mortality of seed of little mallow at 72 C, California burclover seed (without burs) at 85 C, rhizomes of bermudagrass at 69 C, and nutlets of yellow nutsedge at 70 C. These studies indicate that reinfested, aged mulch does not produce sufficient heat to destroy weed propagules and, therefore, may become a weed-carrying substrate. Fresh mulch should be mixed to expose surviving weed propagules at shallow depths to lethal temperatures found at 30 cm or deeper in fresh mulch piles.

A field trial was conducted for 6 yr (1998 through 2003) at Scottsbluff, NE, to measure weed shifts following multiple applications of two rates of glyphosate or alternating glyphosate with nonglyphosate treatments in continuous corn or in a crop rotation of corn, sugarbeet, and spring wheat with all three crops resistant to glyphosate. After 6 yr, plant densities of common lambsquarters, redroot pigweed, hairy nightshade, and common purslane increased in the crop-rotation treatment compared with continuous corn. There were four weed control subplot treatments consisting of two in-crop applications of glyphosate at 0.4 or 0.8 kg ae/ha each spring, alternating two applications of glyphosate at 0.8 kg/ha one year with a nonglyphosate treatment the next year, or a nonglyphosate treatment each year. The composition of the weed population averaged across all four treatments shifted from kochia and wild proso millet to predominately common lambsquarters. After 3 yr of using glyphosate at 0.4 kg/ha twice each year, common lambsquarters density increased compared with that in the 0.8 kg/ha rate of glyphosate or alternating glyphosate treatments. By the sixth year, the density of common lambsquarters in the glyphosate at 0.4 kg/ha treatment had increased to the extent that corn grain yield was reduced 43% compared with corn grain yield in the 0.8 kg/ha glyphosate treatment. Using glyphosate at either rate for 6 yr decreased the densities of kochia, wild proso millet, and longspine sandbur, did not alter densities of redroot pigweed and green foxtail, and increased the density of hairy nightshade. In the low-rate treatment of glyphosate, the number of common lambsquarters seeds in the seed bank were 134 seeds/kg soil in 1998, declined to 15 seeds/kg by 2002, but began to increase in 2003 as the densities of plants not controlled by glyphosate increased.

Studies were conducted to evaluate density-dependent effects of Palmer amaranth on weed and peanut growth and peanut yield. Palmer amaranth remained taller than peanut throughout the growing season and decreased peanut canopy diameter, although Palmer amaranth density did not affect peanut height. The rapid increase in Palmer amaranth height at Goldsboro correspondingly reduced the maximum peanut canopy diameter at that location, although the growth trends for peanut canopy diameter were similar for both locations. Palmer amaranth biomass was affected by weed density when grown with peanut. Peanut pod weight decreased linearly 2.89 kg/ha with each gram of increase in Palmer amaranth biomass per meter of crop row. Predicted peanut yield loss from season-long interference of one Palmer amaranth plant per meter of crop row was 28%. Palmer amaranth seed production was also described by the rectangular hyperbola model. At the highest density of 5.2 Palmer amaranth plants/m crop row, 1.2 billion Palmer amaranth seed/ha were produced.

Experiments were conducted at the Upper Coastal Plain Research Station near Rocky Mount and at the Peanut Belt Research Station near Lewiston-Woodville in 2002 and 2003. Peanut injury was minimal (< 5%) with all soil-applied programs. S-Metolachlor PRE alone or in mixture with sulfentrazone, diclosulam, or flumioxazin controlled annual grasses similarly (66 to 87%). The addition of imazapic plus 2,4-DB POST increased annual grass control (> 93%). Sulfentrazone or diclosulam in mixture with S-metolachlor were the best PRE options, with 94% and 92% control of yellow and purple nutsedge, respectively, with flumioxazin being least effective at 70%. Diclosulam and flumioxazin in mixture with S-metolachlor were the best PRE options, with 99% and 93%, respectively for common ragweed control, whereas sulfentrazone was the least effective at 65%. S-Metolachlor in mixture with sulfentrazone, diclosulam, or flumioxazin PRE were similar (87 to 90%) for common lambsquarters control. S-Metolachlor in mixture with sulfentrazone, diclosulam, or flumioxazin provided similar levels of entireleaf, ivyleaf, pitted, and tall morningglory control (87, 86, and 87%, respectively) and better than S-metolachlor alone at 64%. Flumioxazin in mixture with S-metolachlor was the best PRE option for control of Palmer amaranth at 96%, whereas diclosulam with S-metolachlor was the best PRE option for control of eclipta at 100%. The prepackaged mixture of acifluorfen and bentazon plus 2,4-DB POST and imazapic plus 2,4-DB POST were similar for all morningglory species (> 96%) and Palmer amaranth control (93 and 97%, respectively). Peanut treated with S-metolachlor plus diclosulam PRE numerically yielded the highest at 3,210 kg/ha, but were statistically equivalent to S-metolachlor plus flumioxazin PRE at 3,040 kg/ha. Peanut treated with imazapic plus 2,4-DB POST yielded the most at 3,400 kg/ha, while peanut treated with a prepackaged mixture of acifluorfen and bentazon plus 2,4-DB POST yielded less (3,070 kg/ha).

Field research was conducted in southern New Mexico to determine the effect of halosulfuron rate, timing of application, and adjuvant on purple nutsedge suppression and chile pepper injury under cultivated conditions. POST-directed treatments of 36 or 53 g ai/ha were applied 2 or 4 wk after thinning direct-seeded chile pepper. Herbicide treatments included a nonionic surfactant or methylated seed oil plus ammonium sulfate plus polyacrylamide polymer. Herbicide rate, adjuvant, and timing of the application had little effect on overall nutsedge suppression; however, herbicide treatment proved to be an important component for managing purple nutsedge in this experiment. Yields were higher with halosulfuron treatment compared with the weedy control. Whereas halosulfuron treatment with 53 g/ha increased chile yield relative to the weedy control, injury and yield results comparing 36 and 53 g/ha rates consistently suggested that halosulfuron should be applied at rates ≤ 36 g/ha in a single application to avoid possible injury. Halosulfuron treatment combined with cultivation and effective crop rotation may reduce the impact of purple nutsedge interference in chile pepper.

This study documents the physiology and genetics of evolved atrazine resistance in a wild radish population from Western Australia. Plant response to atrazine treatment confirmed a high level of resistance in population WARR5. At 0.25 kg atrazine/ha, all plants from a susceptible population were killed, whereas resistant WARR5 was unaffected at the highest dose tested (4 kg atrazine/ha). Leaf photosynthesis in susceptible plants was inhibited after 1 kg atrazine/ha treatment, whereas leaf photosynthesis in WARR5 plants was unaffected. Furthermore, atrazine resistance was maternally inherited. Sequencing of a psbA gene fragment in resistant WARR5 and susceptible plants revealed a single point mutation resulting in a coding change from Ser264 to Gly of the D1 protein in resistant plants. We are confident that this mutation is the basis of resistance to the photosystem II inhibitors in this wild radish population.

Studies were conducted at three locations in North Carolina in 2004 to evaluate density-dependent effects of glyphosate-resistant (GR) corn on GR cotton growth and lint yield. GR corn was taller than GR cotton as early as 25 d after planting, depending on location. A GR corn density of 5.25 plant/m of crop row reduced late season cotton height by 49, 24, and 28% at Clayton, Lewiston–Woodville, and Rocky Mount, respectively, compared to weed-free cotton height. At Clayton, GR corn dry biomass per m crop row and GR corn seed biomass per m of crop row decreased linearly with increasing corn density. The relationship between GR corn and GR cotton yield loss was described by the rectangular hyperbola model with the asymptote (a) constrained to 100% maximum yield loss. The estimated coefficient i (yield loss per unit density as density approaches zero) was 9, 5, and 5 at Clayton, Lewiston–Woodville, and Rocky Mount, respectively. The examined GR corn densities had a significant effect on cotton yield, but not as significant as many other problematic grass and broadleaf weeds.

Invasive annual grasses, such as medusahead, can reduce forage production capacity and interfere with revegetation projects in California rangelands. Because of the taxonomic similarity to other more desirable grasses, achieving selective control of invasive annual grasses can be difficult. In selectivity trials conducted in Yolo and Siskiyou counties, CA, the herbicide imazapic gave control of many nonnative annual grasses yet provided some level of selectivity to specific perennial grasses used in revegetation projects throughout the western United States. The selectivity difference between newly seeded perennial and annual grasses was greater with PRE applications than with POST treatments. Both perennial and annual grasses within the tribe Hordeae were more tolerant to imazapic than other grass species. In addition, field experiments were conducted at three sites in northern California (Yuba, Yolo, and Lassen counties) and one in southern Oregon (Lake County) to test the response of imazapic to varying management conditions. Imazapic was applied PRE in fall (and also spring in Lake County) at rates from 35 to 210 g/ha on undisturbed rangeland, in comparison with rangeland cleared of standing plant material and thatch by either tillage, mowing and raking, or burning. Imazapic generally showed enhanced weed control when applied following disturbance. Rates as low as 70 g/ha, if combined with thatch removal, provided significant suppression of medusahead. In addition, disturbance alone generally reduced medusahead cover in the following year. Although imazapic showed potential for control of medusahead and other annual grasses, its selectivity window was relatively narrow.

Field experiments were conducted in Alabama during 1999 and 2000 to test the hypothesis that any glyphosate-induced yield suppression in glyphosate-resistant cotton would be less with irrigation than without irrigation. Yield compensation was monitored by observing alterations in plant growth and fruiting patterns. Glyphosate treatments included a nontreated control, 1.12 kg ai/ha applied POST at the 4-leaf stage, 1.12 kg/ha applied DIR at the prebloom stage, and 1.12 kg/ha applied POST at 4-leaf and postemergence directed (DIR) at the prebloom cotton stages. The second variable, irrigation treatment, was established by irrigating plots individually with overhead sprinklers or maintaining them under dryland, nonirrigated conditions. Cotton yield and all measured parameters including lint quality were positively affected by irrigation. Irrigation increased yield 52% compared to nonirrigated cotton. Yield and fiber quality effects were independent of glyphosate treatments. Neither yield nor any of the measured variables that reflected whole plant response were influenced by glyphosate treatment or by a glyphosate by irrigation interaction.

Field experiments were conducted to evaluate weed control and cotton response to glyphosate or glufosinate applied alone or with residual herbicides applied in the last POST-directed application (LAYBY) in glyphosate- and glufosinate-resistant cotton. Glyphosate (0.86 kg ae/ha) or glufosinate (0.47 kg ai/ha) were applied alone over the top of glyphosate- or glufosinate-resistant cotton early POST (EPOST) followed by (fb) late POST (LPOST) fb one of the herbicides applied either alone or with a residual herbicide at LAYBY. Glyphosate- and glufosinate-based treatments were applied only to glyphosate- and glufosinate-resistant cotton, respectively. Residual herbicides evaluated included prometryn (1.12 kg ai/ha), fluometuron (1.12 kg ai/ha), diuron (1.12 kg ai/ha), oxyfluorfen (1.12 kg ai/ha), pendimethalin (1.0 kg ai/ha), prometryn + trifloxysulfuron (1.33 kg ai/ha + 12 g ai/ha), or linuron + diuron (0.56 + 0.56 kg ai/ha). Glyphosate-and glufosinate-based weed management systems with and without residual LAYBY herbicides resulted in little to no injury to cotton. Three applications of glyphosate or glufosinate alone provided better full-season control of most species when compared to two applications of either herbicide. The addition of a residual herbicide to glyphosate or glufosinate at LAYBY did not improve cotton yields, but did improve overall control of barnyardgrass and yellow nutsedge and reduced weed dry biomass present at time of cotton harvest when compared to three applications of glyphosate or glufosinate alone.

Producers in the Great Plains are exploring alternative crop rotations with the goal of reducing the use of fallow. In 1990, a study was established with no-till practices to compare eight rotations comprising various combinations of winter wheat (W), spring wheat (SW), corn (C), chickpea (CP), dry pea (Pea), soybean (SB), or fallow (F). After 12 yr, we characterized weed communities by recording seedling emergence in each rotation. Downy brome, cheat, redroot pigweed, and green foxtail were the most common weeds observed. Weed community density was highest for W–CP, being 13-fold greater than with Pea–W–C–SB. Downy brome and cheat were rarely observed in rotations where winter wheat was grown only once every 3 or 4 yr; in contrast, density of the brome species was 75-fold greater in W–CP. Warm-season weeds were also affected by rotation design; density of redroot pigweed and green foxtail was sixfold greater in W–C–CP compared with Pea–W–C–SB or W–F. One rotation design that was especially favorable for low weed density was arranging crops in a cycle of four, with two cool-season crops followed by two warm-season crops.

Field experiments were conducted at five sites in Nebraska in 2000 and 2001 to determine the effect of planting depth and isoxaflutole rate on the response of an isoxaflutole-sensitive corn hybrid, ‘Pioneer 33-G’ across variable environments. Corn was planted at depths of 2.5 and 5.0 cm, and isoxaflutole was applied PRE at the recommended (1×) and twice the recommended (2×) rate. The effects of planting depth and herbicide rate on injury varied considerably across site–years. When injury was evident, it was generally greater at the high rate of isoxaflutole (2×) and at the shallow planting depth (2.5 cm). In most site–years, corn recovered from early season injury, and yields were not reduced, except at Scottsbluff, NE, and North Platte, NE, where soils were lower in organic matter and higher in pH. Isoxaflutole rates should be carefully selected for soils with low organic matter and high pH.

Bamboos are grass species that can escape cultivation and invade lawns, landscapes, and other areas. Limited information is available on ways to control invasive bamboo species. Greenhouse and field studies were initiated to determine the level of bamboo control provided by a single application of selected PRE and POST herbicides. Bamboo species included in the study were golden bamboo in greenhouse experiments and red-margined bamboo in field experiments. In greenhouse trials, MSMA, quinclorac, dithiopyr, clethodim, fenoxaprop, and sethoxydim did not control either species. Glyphosate, glufosinate, and fluazifop significantly reduced bamboo-shoot fresh weight, although regrowth occurred after a single application. In field trials, bamboo control with dichlobenil in the 2002 and 2004 experiments was less than 23%. For the study initiated in 2002, glyphosate and imazapyr provided 76% and 98% bamboo control, respectively, at 58 wk after treatment (WAT). By 161 WAT (approximately 3 yr after treatment), bamboo-control ratings were 40% with glyphosate and 85% with imazapyr. For the study initiated in 2004, at 61 WAT, glyphosate and imazapyr provided 46 and 88% control of bamboo, respectively.

Three studies were conducted to develop pollen tests for the screening of acetyl coenzyme-A carboxylase (ACCase) target-site resistance in a biotype of johnsongrass. The assays were based on germination of johnsongrass pollen in media supplemented with clethodim. Two different methods were used to evaluate pollen germination—a visual assessment and a spectrophotometric assay. The response of pollen to the germination media was linear for 16 h. At 6 h after treatment, absorbance at 500 nm was nearly 0.5; consequently, 6 h was chosen to conduct the pollen assays using the spectrophotometer. Both assessment methods differentiated the susceptible (S) and resistant (R) biotypes. Pollen from the susceptible biotype of johnsongrass was strongly inhibited by increasing concentrations of clethodim, with a GR50 of 25.8 ± 0.6 (SE) µM and GR50 of 16.4 ± 1.7 (SE) µM clethodim by visual assessment and spectrophotometric assessment, respectively. Minimum R/S values were > 3.9 by visual assessment and > 6.1 by spectrophotometric assessment. ACCase target-site resistance is expressed in johnsongrass pollen.

A field study was conducted at two sites in southwestern Ontario, Canada, from 2002 to 2005 to assess the cumulative effects of different weed management strategies applied to the same plots over time in a corn–soybean rotation. These strategies included a POST broad-spectrum herbicide applied at recommended or reduced rates and use of a computer decision aid to select the POST herbicide having the greatest net return (economic threshold approach) based on weed populations before treatment or seed-bank estimates. Four years after study initiation, increased weed populations were observed only in the nontreated control. The reduced rate and economic threshold approaches did not lead to increased weed seed banks compared with the recommended rate of a broad-spectrum herbicide. Reductions in herbicide rate were more effective than the use of economic thresholds for reducing the risk of environmental impact in corn and soybeans. Weed populations in this study were generally above threshold levels, and herbicides selected by the computer decision aid tended to be older, less-expensive herbicides with high use rates. Recommendations by the computer decision aid were generally more effective and more profitable when based on weed populations before treatment than when based on seed-bank estimates.

Field observations of morningglory (Ipomoea spp.) showed that many plants grew out from places of comparable competitive advantage (alleys in field experiments with little or no vegetation) into neighboring plants or structures that provided climbing support. Of 223 native morningglory plants growing in rows and row middles in a 121-m2 area within established corn research plots that contained no other weeds, 68% of the mature plants climbed up corn. More significant, of the 152 climbing morningglory plants, 96% grew toward and climbed the row in its closest proximity instead of growing across the row middle. Greenhouse and field experiments were initiated to determine whether morningglory grew preferentially toward certain colored structures or corn plants. Greenhouse-grown ivyleaf morningglory displayed varying frequency in locating and climbing toward black (17%), blue (58%), red (58%), white (67%), green (75%), and yellow (75%) stakes or corn (92%). Pots containing black stakes had the fewest climbing morningglory plants. In the field study, fewer ivyleaf morningglories climbed black structures compared with white- or green-colored structures or corn. The morningglory initial planting distance from colored structures or corn was also significant in the percentage of ivyleaf morningglories that exhibited climbing growth and in its final weight; morningglories that successfully located and climbed structures or corn weighed more and produced more seed than morningglories that remained on the ground. Ivyleaf morningglory appears to respond to spatial distribution of surrounding objects and possibly uses reflectance to preferentially project its stems toward a likely prospective structure for climbing.

A reason given by cash-grain farmers for not using manure from neighboring livestock operations is that manure may cause greater field weediness. To address this concern, trials were established in corn on 11 cash-grain farms, in which manure from six nearby dairy farms was spread for the first time in at least 10 yr. A split-plot design was used in which manured and nonmanured treatments were established as whole-plots, and split-plot treatments were either with or without the farmer's regular weed control. In the multisite analysis, weed seedling density at the time of corn emergence was not greater in the manured vs. nonmanured treatments. At 7 to 8 wk following planting, weed density was not greater in the manured plots. Just before corn canopy closure, weed biomass also did not differ between manured and nonmanured treatments. Although neither weed species richness nor species diversity differed significantly between manured and nonmanured treatments, these measures did have significant environment-by-manure interactions, indicating that weed species distributions responded differently to manure across the different trial environments. However, farmers' weed control practices were highly successful in both the manured and nonmanured plots. Large portions (280 m2) of all whole plots were visually inspected for introduced weed species after all weed control practices had been completed. The manured treatments did not differ significantly in the set of species observed, suggesting that manure did not introduce new weed species. Thus, this exploratory study showed that, contrary to some farmers' concerns, an application of dairy manure neither increased field weediness nor required alterations in the farmers' weed control programs.

Proso and foxtail millets are regionally important dryland crops for the semiarid portions of the Central Great Plains. However, few herbicides are registered for use in either crop. The efficacy of carfentrazone was studied in proso millet from 2003 through 2005 at the University of Nebraska High Plains Agricultural Laboratory located near Sidney, NE, and in foxtail millet in 2004 and 2005 at the University of Wyoming Sustainable Agriculture Research and Extension Center near Lingle, WY. Carfentrazone was applied POST at 9.0, 13.5, and 18.0 gai/ha with combinations of 2,4-D amine, prosulfuron, and dicamba. Although leaves of treated plants exhibited localized necrosis, leaves emerging after treatment were healthy. Grain and forage yields were not affected by the application of carfentrazone. Dicamba and 2,4-D amine provided visual control of 30% or less for buffalobur. Adding carfentrazone to one or both of these herbicides improved buffalobur control to 85% or greater. Carfentrazone applied at 18.0 g/ha improved Russian thistle, kochia, and volunteer sunflower control in 2003, when plants were drought-stressed, but did not help with these and other weeds during wetter years. Carfentrazone provides proso millet producers with a way to selectively control buffalobur, a noxious weed in several western states. In foxtail millet, carfentrazone provides POST broadleaf weed control with little risk for serious crop injury. Crop injury has been a concern with 2,4-D, which is currently the only other herbicide registered for use in foxtail millet.

Field studies were conducted from 2000 to 2002 to determine whether glyphosate applied during periods of low temperature (< 10 C) provides effective control of garlic mustard without injury to nontarget native herbs. A 1% glyphosate solution was applied on three dates between November and March in 2000 to 2001 and 2001 to 2002, when average daily temperatures ranged from −4.2 to 7 C. Glyphosate reduced the population density of prereproductive springtime garlic mustard infestations, regardless of application timing. During the primary bolting period (April to June), mortality of garlic mustard rosettes in sprayed plots was 87 to 94%, whereas mortality in nontreated plots was 12% in the first year and 41% in the second. Nontarget native herbaceous species were not injured by the cold-weather herbicide applications and exhibited higher springtime densities than in the nontreated plots. By targeting garlic mustard rosettes during the part of the year when most other plant species are dormant, managers can selectively control garlic mustard without damage to native herbs and, thereby, increase forest restoration success.

An experiment was conducted at two locations in 2003 and 2004 to determine the timing and rate of thifensulfuron that is safe to use on sweetpotato. Thifensulfuron was applied 1, 2, and 4 wk after transplanting (WAP) in 2003 and 4, 6, and 8 WAP in 2004. Within each timing, thifensulfuron treatments were 1.1, 2.1, 3.2, 4.3, and 8.5 g ai/ha plus a weed-free control. The 1 and 2 WAP timings of thifensulfuron reduced the yield of number 1 roots greater than 25%. The 4, 6, and 8 WAP timings had less than 15% reduction in yield, with the 6 WAP timing reducing number 1 roots and total yield 10% or less. When 4.3 g/ha of thifensulfuron was applied 4 WAP, total yield was reduced 13%. The 6 and 8 WAP timings had little yield reduction, with no rate response observed. Application of 4.3 g/ha of thifensulfuron at 6 WAP would allow for control of problematic weed species while limiting potential yield loss. Yield loss from a 4 WAP application of thifensulfuron may in fact be a delay in crop maturity that could be recovered if the sweetpotato harvest was delayed to allow for the optimal amount of number 1 grade roots to be produced.