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Physiological and Molecular Characterization of Atrazine Resistance in a Wild Radish (Raphanus raphanistrum) Population

Published online by Cambridge University Press:  20 January 2017

L. J. Shane Friesen  and
Stephen B. Powles
L. J. Shane Friesen
Affiliation:
Western Australian Herbicide Resistance Initiative, The School of Plant Biology, University of Western Australia, 35 Stirling Highway, Crawley, WA, Australia 6907
Stephen B. Powles*
Affiliation:
Western Australian Herbicide Resistance Initiative, The School of Plant Biology, University of Western Australia, 35 Stirling Highway, Crawley, WA, Australia 6907
*
Corresponding author's E-mail: [email protected]
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Abstract

This study documents the physiology and genetics of evolved atrazine resistance in a wild radish population from Western Australia. Plant response to atrazine treatment confirmed a high level of resistance in population WARR5. At 0.25 kg atrazine/ha, all plants from a susceptible population were killed, whereas resistant WARR5 was unaffected at the highest dose tested (4 kg atrazine/ha). Leaf photosynthesis in susceptible plants was inhibited after 1 kg atrazine/ha treatment, whereas leaf photosynthesis in WARR5 plants was unaffected. Furthermore, atrazine resistance was maternally inherited. Sequencing of a psbA gene fragment in resistant WARR5 and susceptible plants revealed a single point mutation resulting in a coding change from Ser264 to Gly of the D1 protein in resistant plants. We are confident that this mutation is the basis of resistance to the photosystem II inhibitors in this wild radish population.

Keywords

Atrazinemetribuzinwild radish, Raphanus raphanistrum L. RAPRAHerbicide resistancepsbA mutationtriazine resistance
Type
Research
Information
Weed Technology , Volume 21 , Issue 4 , December 2007 , pp. 910 - 914
Copyright
Copyright © Weed Science Society of America 

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