Greenhouse experiments were conducted to determine effects of watering frequency, drought stress, and a combination of watering frequency and glyphosate on growth of field bindweed. Watering daily, twice weekly, or weekly to field capacity affected field bindweed grown from seed differently than plants grown from root runner segments. Shoot and root dry matter of plants grown from seed increased when plants were watered frequently but dry matter allocated to root runners did not change when plants were watered less frequently. Plants grown from root runner segments increased their shoot and root runner dry matter when watered frequently, but root dry matter was not affected by watering frequency. Plants grown from seed had highest regrowth when watered weekly and plants grown from root runner segments had higher regrowth when watered daily. Drought reduced survival rate and dry matter of surviving plants. Frequency of watering and whether plants began from seeds or root runner segments influenced glyphosate action on field bindweed. No regrowth was observed 6 months after 1.5 kg ai/ha of glyphosate was applied to plants that had been grown for 8 weeks under three different watering frequencies.

The uptake of 2-chloro-4-(ethylamino)-6-(isopropylamino)-s-triazine (atrazine) was studied in sudangrass [Sorghum sudanense (Piper) Stapf, var. Piper], grain sorghum [Sorghum bicolor (L.) Moench], and corn (Zea mays L.) to find whether differences in atrazine uptake exist among these species. The uptake of atrazine followed closely the growth patterns of corn, sorghum, and sudangrass during the first 5 weeks of growth. Concentration of 14C from ring-labeled 14C-atrazine in the soil reached a peak in corn, sorghum, and sudangrass plants after 2 weeks of growth and then declined. The 14C concentrations were two to three times greater in sorghum and sudangrass than in corn throughout the 5-week period. Atrazine uptake per gram of plant growth by these crops was directly proportional to the concentration of atrazine in the soil and the proportionality factors were in the order: sudangrass < sorghum ≫ corn. Total uptake and the total growth were in order: corn ≫ sorghum = sudangrass. In a study where relative humidity was a variable, the amount of atrazine absorbed per ml of water was inversely related to total water usage.

Fluazifop-butyl {(±) butyl 2-[4-[[5-(trifluoromethyl)-2-pyridinyl] oxy] phenoxy] propanoic acid}, haloxyfop-methyl {methyl-2-[4-[[3-chloro-5-(trifluoromethyl-2-pyridinyl] oxy] phenoxy] propanoic acid}, and sethoxydim {2-[1-(ethoxyimino)-butyl]-5-[2-ethylthio)propyl-3-hydroxy-2-cyclohexen-1-one}exhibited soil herbicidal activity to annual grass species in greenhouse and field experiments. Control of forage sorghum [Sorghum bicolor (L.) Moench. ‘Rox Orange’] with all three herbicides was greater when seeds were planted at or near the soil surface than when planted 2, 4, or 6 cm deep. Imbibition of the herbicides by forage sorghum and soybean [Glycine max (L.) Merr.] seeds did not reduce germination at concentrations of 10-3 M or lower, but forage sorghum seedling survival and vigor were reduced by herbicide concentrations as low as 10-7 M. Fluazifop-butyl, haloxyfop-methyl, and sethoxydim were more phytotoxic when applied to the root zone than the shoot zone. Haloxyfop-methyl exhibited the longest soil residual control in the field followed by fluazifop-butyl and sethoxydim. Haloxyfop-methyl and fluazifop-butyl controlled simulated shattercane [Sorghum bicolor (L.) Moench. ♯3 SORVU] in soybeans with preemergence and early postemergence applications, whereas control from similar applications of sethoxydim was limited and variable.

A study was conducted to examine the effects of residence time and temperature on atrazine extractability and degradation in a sediment generated from Emporia sandy loam soil submerged under a water column. Sediments amended with 2.8 μg of atrazine were incubated at two temperatures (5 and 24 C), sampled at 0, 14, 28, 112, 168, 252, and 336 d, and then sequentially extracted once with 80 ml 0.01 M CaCl2 aqueous solution, three times with 25 ml of methanol/water mixture (4:1, v/v), and three times with 25 ml of methanol/water/formic acid (20:5:1, v/v pH 3.2) for a total of seven times. Extracts were analyzed for atrazine and metabolites using reverse phase high-performance liquid chromatography. The rate of bound residue formation was greater at 24 than at 5 C. About 30 and 60% of the 14C was bound (nonextractable) to sediments at 5 and 24 C, respectively. Water was less effective in extracting 14C residues from sediments incubated at 24 C than at 5 C. Acidification of the methanol/water mixture with formic acid during the last extraction step improved the removal of hydroxyatrazine from sediment. Atrazine degraded primarily to hydroxyatrazine (HA) and to a lesser extent to deethylatrazine (DEA). Although no DEA was found, HA at 43 μg kg−1 of sediment was detected 112 d after treatment at 5 C, indicating that chemical degradation could occur at a low temperature if sediments are incubated for extended periods of time. At 24 C, HA was found within 28 d and reached the maximum level at day 252. The results suggested that HA is the major metabolite of atrazine in the sediment; a significant fraction of the residue was unavailable for extraction, and this unextractable portion increased with residence time.

Two-level fractional factorial design experiments were conducted in growth chambers to determine the influence of environmental factors and adjuvants on the foliar penetration of the sodium salt of acifluorfen in velvetleaf. Relative humidity (RH) was found to have the greatest effect on penetration. Temperature was the second most important factor. Although soil moisture significantly affected penetration, this effect was much less than that produced by RH and temperature. Diammonium phosphate (DAP) was the most effective adjuvant tested. The use of DAP at 1.12 kg/ha significantly increased penetration. The effect of DAP was less than either RH or temperature. Oxysorbic (5 POE) surfactant also significantly increased penetration. Glycerol and oxtoxynol (7 to 8 POE) surfactant had no effect on penetration. In separate experiments, acifluorfen penetration at low and high RH and with the addition of DAP was measured to verify these results. Acifluorfen penetration into velvetleaf increased approximately ninefold with changes in RH from low to high. Penetration increased sevenfold when DAP was added. DAP was by far the most effective adjuvant increasing acifluorfen penetration into velvetleaf under conditions of low RH. Oxysorbic (5 POE) surfactant slightly increased penetration under these conditions.

Weedy rice (Oryza sativa L.) is among the most problematic weeds in rice (Oryza sativa L.) production. The commercialization of herbicide-resistant (HR) rice nearly two decades ago provided an effective tool to manage weedy rice; however, resistance evolution and volunteer HR hybrid rice kept weedy rice at the forefront of rice weed control needs. This research aimed to assess the prevalence and severity of weedy rice infestations, identify production practices that may have contributed to an increase in weedy rice, and determine control strategies that may still be effective on weedy rice across Arkansas and adjacent U.S. Midsouth locales. Two questionnaires, one for rice growers and consultants and one for County Extension agents (CEAs), were distributed through email and physical copies in 2020. Thirty-three respondents returned the rice grower (25) and consultant (8) survey, representing 26 and 7 counties in Arkansas and the Missouri Bootheel area, respectively, as well as four parishes in northeast Louisiana. Eighteen respondents returned the CEA survey. Respondents ranked weedy rice the third most problematic weed in rice, behind Echinochloa spp. and Cyperus spp. The most common infestation levels reported in 78% of fields was less than 12 m−2. Crop rotation (64% growers/consultants, 50% CEAs) and HR rice technology (27% growers/consultants, 50% CEAs) were the top two most-effective methods for weedy rice management, respectively. Tillage and crop rotation practices significantly influenced weedy rice infestation. Rice–soybean [Glycine max (L.) Merr.] rotation had the lowest weedy rice infestation compared with rice monoculture and other crop rotation practices. Crop rotation was not practiced on 26% of reported fields, primarily due to poor drainage. The imidazolinone (IMI)-resistant rice technology was still effective (>70% control) in 60% of fields, but quizalofop-resistant rice is needed to control IMI-resistant weedy rice. Overall, weedy rice remains a challenging weed in rice production.

On three soils, 3-amino-2,5-dichlorobenzoic acid (amiben) at 1.5 and 15 ppmw did not inhibit the nitrifying microflora, but 150 and 1500 ppmw significantly reduced the nitrate nitrogen produced by the soil microflora. No breakdown of amiben in liquid media was detected during 70 days. Amiben had a net minus charge in solution, and traveled at about the same rate on paper strips in electrophoresis as the dye brilliant blue. The organic matter content of soil was the property most closely associated with adsorption of 14C-labeled amiben. Only kaolinite, of four clays studied, adsorbed amiben significantly. There was no volatilization of amiben from stainless steel planchets stored at temperatures of 15, 25 or 35 C and relative humidities of 0, 33, 66, or 100 per cent.

Preemergence control of itchgrass in sugarcane with nonincorporated pendimethalin and prodiamine increased as rate for each herbicide increased from 2.2 to 3.4 kg ai ha-1. Itchgrass control at 3.4 kg ha-1 was similar for both herbicides, ranging from 78 to 94% (86% mean) for pendimethalin in six field experiments and 83 to 91% (86% mean) for prodiamine in three experiments. Incorporated trifluralin at 2.2 kg ha-1, the standard, gave 99% mean control of itchgrass. None of the nonincorporated treatments with pendimethalin or prodiamine consistently prevented a decrease in the sugarcane stalk population at harvest. Asulam applied postemergence at 3.7 kg ae ha-1 controlled itchgrass that survived the preemergence herbicides, and sugarcane stalk populations were maintained. Preemergence control of johnsongrass with nonincorporated treatments of pendimethalin or prodiamine at 2.8 kg ha-1, in a mixture with atrazine at 2.2 kg ai ha-1, ranged from 86 to 95% (89% mean) for pendimethalin and 80 to 95% (88% mean) for prodiamine. Metribuzin at 2.2 kg ha-1, the standard, gave 97% mean control of johnsongrass. Mixtures of pendimethalin with metribuzin or terbacil generally gave more effective control of johnsongrass than mixtures with atrazine. When used in conjunction with other herbicides, nonincorporated pendimethalin and prodiamine were effective alternatives to soil-incorporated treatments for weed control in sugarcane.

Prodiamine is a mitotic inhibiting herbicide regularly used to control annual bluegrass PRE. A population of annual bluegrass not controlled by prodiamine at 1,120 g a.i. ha−1 was identified on a golf course in Alcoa, TN, in 2012. A whole-plant hydroponics bioassay was used to screen this biotype for prodiamine resistance (PR) compared with a known susceptible population (SS). Multitiller (i.e., > 4 tillers) PR and SS annual bluegrass plants were established in hydroponic culture and exposed to 0, 0.001, 0.01, 0.10, 1.0, and 10.0 mM prodiamine. Exposure to prodiamine at 0.001 mM reduced root growth of the SS biotype to 26% of the nontreated check (i.e., 0 mM prodiamine) but had no effect on the PR biotype. When exposed to 10 mM prodiamine, root growth of the PR biotype was reduced to 24% of the nontreated check compared with 9% for the SS biotype. I 50 values for the PR and SS biotypes were 0.04 and 2.8 × 10−6 mM prodiamine, respectively. The PR biotype measured lower in plant height and leaf width than the SS population. In field trials, prodiamine at 560, 840, 1,120, and 1,400 g ha−1 only controlled the PR biotype 0 to 22%. PRE applications of the cellulose biosynthesis inhibitor indaziflam at 35, 52.5, and 70 g a.i. ha−1 controlled this PR biotype 70 to 97%. This marks the second instance of annual bluegrass developing resistance to prodiamine in Tennessee during the past 5 yr. Future research should evaluate indaziflam efficacy for control of other prodiamine-resistant biotypes of annual bluegrass as well as annual bluegrass biotypes resistant to herbicidal inhibitors of 5-enolpyruvylshikimic acid-3-phosphate synthase, acetolactate synthase, and photosystem II.

Greenhouse and laboratory studies were conducted to examine the effects of site of plant exposure to glyphosate spray on efficacy, absorption, and translocation in pitted morningglory. Absorption of 14C-glyphosate in four-leaf pitted morningglory gradually increased with time from 19% at 1 h after treatment (HAT) to 44% at 192 HAT. The amount of 14C translocated with time ranged from 0.4% at 1 HAT to 25% at 192 HAT. Vining 1-m tall plants were controlled 75 to 100% when the top-, middle-, bottom one-third, or entire plant was treated with 1.38 or 2.76 kg ha−1 glyphosate, with control affected more by glyphosate rate than plant section exposed to glyphosate spray. Absorption of 14C-glyphosate at 96 HAT was similar whether it was applied to the top-, middle-, bottom one-third, or entire plant of 1-m tall pitted morningglory. The amount of 14C translocated out of the treated area (5 to 6%) did not differ whether it was applied to top-, middle-, or bottom one-third plant section. Results indicate that absorption and translocation of 14C-glyphosate in pitted morningglory was rapid and increased with time. Treating any one-third section of pitted morningglory plants was as effective as entire plant exposure, and control with glyphosate is more affected by rate than the degree of plant exposure to glyphosate.

The intensification of farming practices has reduced weed infestations, but it has also led to a reduction in weed diversity and changes in species composition. These effects are well described for aboveground flora; however, it is less clear how these effects might be expressed in the soil weed seedbank. We evaluated the effects of different long-term farm management strategies on the weed seedbank abundance, diversity, and community composition in the DOK (bioDynamic, bioOrganic, and Konventionell) field trial established in 1978 at Therwil, Switzerland. The trial compares biodynamic, organic, and conventional farming systems, which mainly differ in fertilization, weed control strategies, and pest control. The species richness and seed abundance of the weed seedbank were higher in the organic and biodynamic systems compared with the conventional ones. The different farming systems favored shifts in species assemblages, because specific management practices, such as herbicide application and type of fertilization, acted as filters that selected against certain species but promoted others that were more adapted.

Three-quarter inch seedlings of northern nutsedge (Cyperus esculentus L.) were transplanted into the field where numbers of plants, inflorescences and the rate of spread were recorded. Seed was collected from each plot and germination tests conducted. During one growing season, a single seedling developed into a stand of plants that produced a yield of 90,000 seeds with a germination of 51%.

Cotton plants were treated with C14-ring-labeled 2-methylmercapto-4,6-bis(isopropylamino)-s-triazine (prometryne) which was soil-incorporated or placed in solution cultures. C14-labeled material accumulated in the lysigenous glands of roots, stems and leaves. No evolution of C14-carbon dioxide could be detected. Unaltered C14-prometryne was present in stems and leaves 23 weeks after treatment, but no radioactive materials could be detected in the seed. Small amounts of hydroxypropazine were isolated from the stems and leaves of plants grown in soil, but none was detected in plants grown in solution culture. Both water-soluble and acetone-insoluble radioactive materials accumulated in the leaves, stems and roots of all treated plants.

Yellow nutsedge (Cyperus esculentus L. # CYPES) was hoed in two cotton (Gossypium hirsutum L. ‘Acala SJ-2′) fields each year (1979 to 1981) for 2 to 12 weeks after crop emergence to evaluate the influence of several nutsedge-free periods on the growth and reproduction of nutsedge and yields of seed cotton. Compared to the control (plots cultivated but not hoed), a 2-week nutsedge-free period reduced the average number of nutsedge shoots at harvest by 52% (61 vs 29 shoots/m2). Additional hoeings for 4 to 12 weeks further reduced populations by 67 to 87%. Compared to tubers counted in the spring, populations of tubers in control plots increased 3- to 12-fold by harvest. However, compared to control plots, two weekly hoeings reduced tuber populations at harvest by 70 to 90% (treatment average of 884 vs 221 tubers/m2), and additional hoeings tended to reduce, or reduced, tuber populations further (75 to 98% reduction). When cotton yields were reduced in control plots in 1979 and 1980, a 2- to 6-week nutsedge-free period substantially decreased yield losses. Averaged over six locations, yields in plots with a 4- to 12-week nutsedge-free period were greater than yields of control plots.

Eleven herbicide systems were evaluated from 1981 to 1983 for cotton (Gossypium hirsutum L. ‘Stoneville 825’) planted no-till directly into cover crops or winter fallow and for cotton planted following conventional tillage. Herbicide systems consisted of paraquat (1,1’-dimethyl-4,4’-bipyridinium ion) or glyphosate [N-(phosphonomethyl)glycine] and/or residual herbicides applied prior to crop emergence. Some systems also included early postemergence or postemergence-directed applications. Cover crops were crimson clover (Trifolium incarnatum L.), hairy vetch (Vicia villosa Roth.), and rye (Secale cereale L.). Soil cover in fallow treatments was comprised mainly of cotton stalk residue. Conventional tillage and seedbed preparation included fall moldboard plowing and spring disking/smoothing. Treatments were maintained in the same site each year. Order of cover crop susceptibility to herbicides applied prior to crop emergence was rye>clover>vetch. In 1981, all residual treatments except cyanazine {2-[[4-chloro-6-(ethylamino)-1,3,5-triazin-2-yl]amino]-2-methylpropanenitrile} alone prior to crop emergence provided better than 80% control of annual weeds. In subsequent years only systems that included applications prior to crop emergence and postemergence-directed herbicides provided acceptable control. From 1981 to 1983, annual grasses increased 20- to 100-fold for systems in which control was poor. Weed control was generally superior in conventional tillage. Vetch adversely affected cotton stands in all 3 yr. Also, in 1982, reduced cotton stands resulted from cyanazine treatments applied prior to crop emergence in clover, vetch, and fallow. Cotton yields were affected by cover dessication, annual weed control, and cotton stands. Buildup of annual weeds reduced yields to near zero for some treatments in 1983.

Sequential application of cycloate (S-ethyl N-ethylthiocyclohexanecarbamate) at 3.4 kg/ha, phenmedipham (methyl m-hydroxycarbanilate m-methylcarbanilate) at 1.1 kg/ha, and trifluralin (α,α,α-trifluoro-2,6-dinitro-N,N-dipropyl-p-toluidine) at 0.6 or 0.8 kg/ha, plus certain mechanical measures, controlled weeds during all periods of the growing season without significant injury to sugarbeets (Beta vulgaris L.). Early-season weed control was complete enough that the new selective mechanical thinners could have replaced labor for thinning. The additional labor required to bring the crop to harvest free of weeds was limited to one late-season hoeing, which required less than 10% of the total labor required in traditional weed control schedules without herbicides.

The influence of ultraviolet-B (UV-B) radiation on seedling growth and leaf surface characteristics of important rangeland weeds Cynoglossum officinale L. (houndstongue), Centaurea diffusa Lam. (diffuse knapweed), and Tragopogon pratensis L. (meadow salsify) was investigated in a greenhouse by exposing seedlings to 0, 7, or 11 kJ m−2 d−1 of biologically effective UV-B radiation. UV-B radiation reduced leaf, stem, and root fresh weights, leaf area, and leaf: shoot ratio and increased shoot dry matter content, specific leaf weight, and leaf greenness of C. officinale seedlings. Leaf, stem, and root fresh weight, and leaf area of C. diffusa seedlings decreased, whereas shoot dry matter content, specific leaf weight, leaf: shoot ratio, and leaf greenness were unaffected. T. pratensis seedlings exposed to UV-B radiation had diminished leaf greenness, but no change was observed in leaf or stem fresh weight, leaf area, or leaf: shoot ratio. Uniseriate trichome abundance in C. officinale seedlings increased and trichome orientation changed in response to UV-B exposure. UV-B treatments decreased uniseriate trichome abundance in C. diffusa; glandular trichome abundance and the ratio of glandular: uniseriate trichomes were not affected. T. pratensis seedlings showed no change in the amount of epicuticular wax on the adaxial leaf surface in response to UV-B irradiation. Shoot dry weight was not influenced by removal of epicuticular wax prior to UV-B exposure. Results suggest that epicuticular wax is not an important factor in acclimation of T. pratensis seedlings to UV-B radiation. Susceptibility to UV-B–induced damage was greatest for C. officinale seedlings and least for T. pratensis seedlings. Modification of plant morphology or reduction in growth induced by enhanced UV-B radiation may influence competitive relationships between rangeland weeds and their associated forage species.

Laboratory experiments were conducted to determine the physiological mechanism of tall morningglory resistance to the experimental cotton herbicide DPX-PE350. Tall morningglory, a resistant species, was compared with entireleaf morningglory, a sensitive species, to evaluate inhibition at the site of action, the acetolactate synthase (ALS) enzyme (E.C.4.1.3.18), by DPX-PE350 as well as uptake, translocation, and metabolism of DPX-PE350. No differences were found between species in the concentration required to inhibit the ALS enzyme by 50% (I50), or in uptake and translocation of the herbicide. Tall morningglory metabolized the herbicide more rapidly than did entireleaf morningglory. Tall morningglory contained 3.6 and 1.4 times more metabolites of DPX-PE350 than did entireleaf morningglory 6 and 24 h after treatment, respectively. Tall morningglory produced an O-desmethyl metabolite from the 3,5-dimethoxypyrimidine moiety of DPX PE350 that was not found in entireleaf morningglory. These data suggest that the ability of tall morningglory to more rapidly metabolize DPX-PE350, possibly through the production of the pyrimidinyldesmethyl metabolite, may be the mechanism of resistance to DPX-PE350.

Thirty-six collections of foxtail barley (Hordeum jubatum L.) from North Dakota, South Dakota, Minnesota, Wisconsin, and Montana varied in susceptibility to 1-(2-methylcyclohexyl)-3-phenylurea (siduron) as determined from radicle elongation of treated seeds. Data from F2 seedlings of crosses between tolerant and susceptible selections indicated that three complementary dominant factors control the inheritance of siduron tolerance in foxtail barley. Evolution of siduron-tolerant foxtail barley could occur from selection pressure.

Simazine (2-chloro-4,6-bis(ethylamino)-s-triazine) at 0.12 to 1.0 ppmw in nutrient cultures of common duckweed (Lemna minor L.), elodea (Elodea canadensis Michx.), and parrotfeather (Myriophyllum brasiliense Camb.) inhibited oxygen evolution within 24 hr. Of the plants studied, the submersed form of parrotfeather exhibited the greatest reduction in apparent photosynthesis as measured by dissolved oxygen in the water. Simazine in nutrient culture without plants remained relatively stable during the treatment period; a slight, but not significant, diminution of chemical was detected after 4 days. Each species, elodea or emersed parrotfeather, reduced the concentration of simazine in solution within 48 hr after treatment.