Low rates of diphenylether herbicides were previously shown to increase efficacy of glyphosate. Experiments were conducted to determine effect of lactofen on uptake of glyphosate and movement into tissues distal from the treatment area as measured by shikimate accumulation. As expected, glyphosate caused shikimate to accumulate, and sublethal rates of lactofen increased this effect. Cuticle abrasion had no effect on activity of glyphosate, alone or with lactofen, suggesting that the influence of lactofen was not in aiding penetration into the leaf.

Intact wheat plants and wheat calli responded similarly to varying concentrations of imazapyr. Fifty percent growth inhibition of wheat callus occurred with 0.05 μM imazapyr after 70 d. As imazapyr concentration increased from 0 to 10 μM, the free isoleucine, leucine, and valine decreased from 160 to 35, 260 to 49, and 310 to 59 pmol mg−1, respectively. Resistant calli, which had relative growth rates exceeding a calculated upper prediction interval, were obtained by in vitro selection at 2 and 5 μM imazapyr. Resistant calli growing on 2 μM imazapyr had free isoleucine, leucine, and valine concentrations intermediate to the control and susceptible callus.

Uptake, translocation, and metabolism of 14C-terbacil was investigated in 12-leaf (tolerant) and 3-leaf (susceptible) field violet plants. Field violets with 12 leaves absorbed less 14C-terbacil g−1 of fresh weight from solution culture than did plants with three leaves. Plants with three leaves translocated twice as much radioactivity to foliage than did plants with 12 leaves. Most 14C in roots (77%) and foliage (57%) of field violet plants with 12 leaves was in polar metabolites. Metabolism studies indicated that most 14C (79%) in foliage extracts from field violet plants with three leaves was 14C-terbacil. Polar metabolites were not detected in roots of field violet plants with three leaves.

A diclofop-methyl-resistant biotype of Italian ryegrass was characterized to determine the expression and inheritance of herbicide resistance and whether this trait was due to the presence of a diclofop-insensitive form of acetyl-coenzyme A carboxylase (ACCase). At the whole plant level, the resistant biotype was > 93-fold more resistant to diclofop-methyl than the susceptible biotype. Crosses of diclofop-resistant and –susceptible plants were performed to produce F1 plants. No maternal effects were evident in responses of reciprocal F1 plants to diclofop. GR50 diclofop rates determined for resistant, F1, and susceptible plants were 15, 6.3, and 0.16 kg ha−1, respectively. F2 populations treated with a 7.5 kg ha−1 rate of diclofop exhibited three injury response phenotypes 3 wk after treatment: a susceptible (S) phenotype which was killed, an intermediate resistance (I) phenotype with severe injury, and a resistant (R) phenotype with little or no injury. Testcross progeny exhibited only I and S phenotypes. Observed segregation of phenotypes in F2 and testcross populations conformed to segregation ratios predicted for a trait with inheritance controlled by a single partially dominant nuclear gene. ACCase activity determined in crude cell-free extracts of resistant, F1, and susceptible biotypes exhibited I50 values of 50, 20, and 0.7 μM diclofop, respectively. A positive relationship between the injury response phenotype and site of action (ACCase) response to diclofop was evident in both F1 and F2 populations. In extracts from R, I, and S phenotype F2 plants, 20 μM diclofop acid inhibited ACCase-mediated incorporation of 14C by 27.1, 45.1, and 78.9%, respectively. The ACCase data are consistent with the hypothesis that diclofop resistance in Italian ryegrass is conferred by a diclofop-insensitive form of ACCase.

Studies were conducted to compare changes in germinability and/or viability of yellow starthistle achenes buried (in packets) in soil or stored dry in the laboratory, and to determine the rate of achene depletion from a soil seed bank. In one study, after 72 mo, 0 to 96% germinable achenes remained in packets buried 5 cm deep, and a mean above 99% after dry storage. In another study, total live (germinable plus viable) achenes declined from 100% at harvest to 77.6% after 24 mo of burial. Germinable pappus-bearing achenes remaining in packets increased with depth of burial. After 12 mo of burial, maximum survival of achenes occurred at depths of 5 cm or more. The density of yellow starthistle achenes and seedlings in a natural soil seed bank declined, when achene rain was prevented, to 3.9 and 1.1% of initial density, respectively, after 36 mo. Decline was attributed primarily to seedling emergence and achene death. Achene density in the upper 2.5 cm of soil appeared to be a good predictor of seedling emergence after autumn rains.

Effects of environmental factors on germination and emergence of hairy beggarticks were examined in laboratory and greenhouse studies. Optimum temperature range for germination of hairy beggarticks was 25/20 to 35/30 C (day/night, 12/12 h). Germination decreased above or below this range. Temperatures below 15/10 C and above 45/40 C were unfavorable for germination. Hairy beggarticks seed can germinate under both a 12-h photoperiod and a 24-h dark regime. Seed germinated 78 to 90% in buffer solutions of pH 4 to 9. Radicle growth was more sensitive to extreme pH than germination. Osmotic stress up to −0.1 MPa had little effect on germination, but less than 3% of the seed germinated at an osmotic stress of −0.75 MPa. Hairy beggarticks seed (13%) germinated at NaCl concentration of 100 mM but failed to germinate at 200 mM NaCl. Maximum emergence occurred when seed were planted less than 1 cm deep. No seedlings emerged when planted 10 cm deep. Flooding even for a day following planting decreased emergence to 25% compared to no flooding (56%). Seedling emergence decreased sharply with a further increase in duration of flooding, and no seedlings emerged when flooding was maintained up to 28 d after planting.

Induction of germination in dormant barnyardgrass seeds by wounding was investigated. Previous research indicated that a volatile compound was emitted during imbibition of wounded caryopses. When wounded caryopses were submerged in agar, total germination and speed of germination were stimulated, and the stimulation was dependent upon the concentration of agar. A twofold increase in germination occurred in 1% agar versus water, and a fivefold increase with caryopses placed in 5% agar. When wounded caryopses were imbibed, there was a fourfold increase in respiration over that of intact caryopses after 1 h. This increased rate of respiration of wounded caryopses continued for 7 h, while there was a gradual increase in respiration of intact caryopses. Carbon dioxide induced intact dormant caryopses to germinate but was not effective in stimulating germination of the seed (spikelet). High levels of abscisic acid found in the hulls of dormant seeds may have prevented the action of carbon dioxide. These results suggest that increased respiration resulting from wounding provides elevated levels of carbon dioxide in the microenvironment of the seed, thus stimulating germination. Removal of the hulls is necessary for germination even in high levels of carbon dioxide.

A study was conducted under controlled environmental conditions to determine the effect of soil temperature, soil moisture, and depth of seed burial on the emergence of redstem filaree. Emergence occurred from 5 to 30 C but was optimal at 5 to 15 C. Redstem filaree emergence was optimal in moist soil with water contents of −0.03 to −0.28 MPa. Emergence progressively decreased as soil moisture was lowered below these levels, with less than 25% emergence attained at −1.53 MPa regardless of soil temperature. Almost no germination was observed in warm (30 C), dry (-1.03 to −1.53 MPa) soils. Rate of emergence was affected more by soil temperature than by moisture. A decrease in temperature from 20 to 5 C increased the time to reach 50% emergence by 6 to 8 d but a decrease in soil moisture from −0.03 to −1.53 MPa increased the time to reach 50% emergence by only 1 to 2 d. Emergence was greatest at soil depths of 1 cm or less. No emergence occurred at depths of 8 cm or below.

Dormant line M73 wild oat caryopses were utilized to develop a system for the culture of excised embryos, to evaluate whether embryo dormancy exists, and to investigate the physiological basis for breaking dormancy. Dormant embryos cultured on N6 medium solidified with 0.25% Bacto agar displayed 70% germination in 2 d compared with approximately 20% for the other gelling agents. The non-plant-based gelling agent gellum at a concentration of 0.25% was selected for further experiments on breaking dormancy after it was determined that concentrations ≥ 0.5% decreased the rate of germination. Amending N6 medium with concentrations of 0.1 to 10 μM gibberellic acid (GA) increased the rate and extent of germination. Embryos treated with 0 to 0.01 μM GA required 6 d to attain 90% germination. Germination of dormant embryos on N6 medium without GA suggested that either true embryo dormancy did not exist in M73 or some constituent of the N6 medium promoted breaking of dormancy. Subsequent experiments indicated that the 88 mM sucrose was the constituent in the N6 medium responsible for breaking dormancy. Concentrations of sucrose from 40 to 200 mM were effective in breaking dormancy. Ten μM GA increased the rate and extent of germination of embryos cultured with 88 to 200 mM sucrose. At 88 mM, fructose, maltose, glucose, and sucrose all broke embryo dormancy. Fructose was the most active soluble sugar for breaking embryo dormancy, promoting nearly 100% germination in 4 d. As with sucrose, there was an interaction between GA and the soluble sugars in breaking dormancy. Ten μM GA with 88 mM fructose provided nearly 100% germination in 1 d. Amylose, but not amylopectin or pullulan, may substitute for soluble sugars. However, with 10 μM GA amylose, amylopectin and pullulan were equally effective in breaking dormancy. Breaking dormancy of embryos on N6 medium was independent of temperatures from 12 to 24 C in the presence of GA, but in its absence the optimum was 12 C. Application of GA to dormant caryopses significantly increased and decreased the level of glucose and sucrose, respectively, in the embryo. Gibberellic acid had a similar effect on glucose and sucrose in the endosperm tissue, except the differences were not significant at all times after treatment. The change in carbohydrate metabolism, especially in embryo tissue, may be important when considered in context with the observation that soluble sugars and GA act independently in breaking dormancy in excised M73 embryos. Breaking wild oat embryo dormancy with GA may be mainly a substitution for sugar requirement.

Three wild oats phenotypes were grown in wheat stands sown at different dates in greenhouse and field trials. Wild oats growth and seed output, and their effects on wheat biomass were not different among phenotypes when wild oats emerged 2 wk after the wheat. In experiments in which wild oats were planted in germinated wheat, one phenotype was shorter, weighed less, and produced fewer seed than the other phenotypes. Another phenotype reduced wheat biomass more than the other phenotypes. Vernalization increased vegetative growth and reduced spikelet production of one phenotype, but had no effect on its competitiveness with wheat.

The effect of black and eastern black nightshade on the amount of photosynthetically active radiation (PAR) intercepted by a processing tomato canopy was studied along with the correlation between PAR and tomato growth and yield. During 1989 and 1990, black and eastern black nightshade were established at densities of 0 to 4.8 m−2 within rows of transplanted, irrigated processing tomatoes. Increasing the density of either nightshade species decreased the number of tomato fruit; however, eastern black nightshade reduced tomato yield more than black nightshade. Eastern black nightshade was taller than the tomatoes, reducing PAR reaching the top of the tomato canopy. PAR reaching the top of the tomato canopy was positively correlated with yield and negatively correlated with eastern black nightshade density. Eastern black nightshade intraspecific competition decreased both stem and berry weight. Black nightshade was never taller than tomatoes and did not affect PAR reaching the top of the crop canopy. Increasing the density of black nightshade decreased berry dry weight but increased the weight of stems and leaves.

Field experiments were conducted in 1986, 1987, and 1988 to evaluate imazethapyr rate and time of application on postemergence control of 24 weed species. Contour graphs were developed that predicted imazethapyr rates required for various levels of weed control based upon weed leaf number at application. Rates below the labeled rate (70 g ha−1) provided 90% or greater control of common cocklebur, smallflower morningglory, and smooth pigweed if applied to 3 true-leaf or smaller weeds and of barnyardgrass, seedling johnsongrass, and Palmer amaranth if applied while weeds were in the cotyledon or 1 true-leaf stage. A rate of 70 g ha−1 provided 90% control of large crabgrass in the 1 true-leaf stage. Entireleaf morningglory, red rice, pitted morningglory, and velvetleaf are not susceptible enough to imazethapyr for 90% or greater control to be obtained with rates lower than 70 g ha−1 at the 1 true-leaf growth stage. These data demonstrate how control data can be used for developing effective reduced-rate herbicide recommendations based on weed leaf number.

The objective of this field research was to compare relative effectiveness and profitability of alternative herbicides applied to the same plots for four consecutive years for controlling and reducing dense Canada thistle infestations in no-till spring wheat. Chlorsulfuron at 30 g ai ha−1 plus nonionic surfactant or clopyralid plus 2,4-D at 70 plus 280 g ae ha−1, respectively, applied annually for 4 yr controlled Canada thistle and was more effective for gradually reducing Canada thistle stands than 2,4-D at 560 g ae ha−1, MCPA plus bromoxynil at 280 plus 280 g ae ha−1, or tribenuron at 20 g ai ha−1. Chlorsulfuron and clopyralid plus 2,4-D also controlled Canada thistle ≥ 90% earlier (by 2 yr) than other treatments. Stochastic dominance analysis, a form of economic analysis, predicted that either chlorsulfuron or clopyralid plus 2,4-D would be preferred by farmers to the untreated check, MCPA plus bromoxynil, or 2,4-D treatments. Chlorsulfuron also would be preferred to clopyralid plus 2,4-D by risk-neutral farmers, whereas clopyralid plus 2,4-D would be preferred to chlorsulfuron by highly risk-averse farmers, those who are most likely to pick only consistently effective herbicides.

Field research was conducted at Hancock, WI, from 1985 through 1987 to evaluate effects of conventional tillage, chisel plow, ridge tillage, and no-tillage systems on population dynamics and control of annual weed species in corn grown continuously on a loamy sand soil without irrigation. In all years of the study, green foxtail densities were greater in chisel plow and no-tillage than in the conventional tillage system, while ridge tillage had densities lower than all other tillage systems. Common lambsquarters density in the chisel plow system reached nearly 500 plants m−2 compared to less than 75 plants m−2 in the other tillage systems when averaged over years. Average redroot pigweed densities in the no-tillage and chisel plow systems were 307 and 245 plants m−2 compared to less than 25 plants m−2 in the conventional and ridge tillage systems. Horseweed was observed only in no-tillage and ridge tillage plots. Green foxtail and redroot pigweed were more difficult to control in chisel plow and no-tillage than in the conventional and ridge tillage systems with several herbicide treatments. Corn yields were not affected by tillage systems under weed-free conditions. Corn yield differences among tillage systems when the same herbicide treatment was applied appeared to be due to differences in weed control.

Cotton yield response was evaluated in 1987, 1988, and 1989 when weeds were managed with preemergence fluometuron [none (0%), band (50%), or broadcast (100%) surface coverage], cultivation (none, one, two, or three times), and postdirected fluometuron + MSMA. Weed densities (primarily prickly sida, morningglories, and hemp sesbania) varied widely among years and were directly related to early season rainfall. Postdirected herbicide application or cultivation(s) had little effect on weed density. The use of a banded fluometuron application reduced weed biomass 28 to 47%. A further decrease was observed when preemergence fluometuron was increased from banded to broadcast coverage. Seed cotton yields were low with no preemergence fluometuron. Banded fluometuron and at least one cultivation had yields similar to broadcast fluometuron only. Cotton yields were related to weed density and weed biomass in a hyperbolic relationship. Low weed densities caused more yield loss per unit weed density than higher densities.

Postemergence and commonly used preemergence plus postemergence herbicide programs were evaluated for weed control in conventional and no-tillage soybeans double cropped after winter wheat in the northern Mississippi River Delta. Broadleaf weed species present varied with tillage, location, and year. Large crabgrass was present in both tillage systems for all locations and years. Conventional tillage usually resulted in higher densities of large crabgrass, common cocklebur, and smooth pigweed. Soil-applied preemergence herbicides alone did not provide season-long ivyleaf and entireleaf morningglory or common cocklebur control in either tillage system. For weed control and soybean yields comparable to weed-free controls, postemergence broadleaf herbicides were required in both tillage systems following the soil-applied herbicide programs, alachlor plus linuron and alachlor plus metribuzin. Postemergence herbicide programs provided excellent season-long annual grass and broadleaf weed control when sethoxydim was applied separately from the broadleaf herbicides. Antagonism of large crabgrass control resulted when sethoxydim was tank mixed with postemergence broadleaf herbicides, compared to separate applications of the postemergence grass and broadleaf herbicides. In three out of four studies, soybean yields in handweeded controls were similar between tillage systems.

Timing and application rates of herbicides were evaluated for control of duncecap and tall larkspur on mountain rangelands. Picloram, triclopyr, glyphosate, and metsulfuron were applied at three rates during three growth stages (vegetative, bud, and flower) to evaluate the rate by growth stage interaction. Picloram was equally effective over all growth stages when applied at 1.1 or 2.2 kg ae ha−1. Metsulfuron was most effective when applied in the vegetative stage; 0.035 kg ai ha−1 killed 95% of duncecap larkspur, but 0.14 kg ha−1 was required to kill the same percentage of tall larkspur. Glyphosate at ≤ 2.2 kg ai ha−1 was least effective when applied in the flower stage compared to earlier growth stages. Triclopyr showed variable control at rates from 1.1 to 4.5 kg ae ha−1.

Fluorescence spectroscopy offers several advantages over other methods of detection and quantitation of chemical compounds. This technique has been underutilized in detection and quantitation of herbicides. The fluorescence properties of 39 herbicides representing several major types of chemistry were determined. The fluorescence of analytical standards was measured in acetonitrile, acetonitrile + water, and acetonitrile + water + strong acid. Fourteen of the 39 herbicides fluoresced to some extent, and seven (bentazon, chloramben, difenzoquat, fluometuron, imazaquin, MCPA, and norflurazon) were identified as good candidates for further method development. The technical advantages and disadvantages of fluorescence spectroscopy of herbicides are discussed.

Leafy spurge is a serious problem because of the speed with which it spreads and the difficulty of controlling it with available means. A rangeland economics model was developed to estimate the economic impacts of leafy spurge infestation on both ranchers and regional economies. A leafy spurge-induced carrying capacity reduction of about 580 000 animal unit months (AUMs), or enough for 77 000 cows, reduced ranchers' annual net income nearly $9 million. Ranchers did not spend another $14 million on input costs, which reduced regional business activity. The regional impacts are about $75 million in reduced business activity for all sectors. These impacts on rancher incomes and regional economies suggest the potential economic returns of leafy spurge control could be substantial.

It has been suggested that soil treated with a herbicide and subsequently carried by wind and deposited on plant foliage can cause crop injury. This study compared foliar uptake and translocation of herbicides applied to plants as an aqueous solution or in herbicide-treated soil. Leaves of 3-wk-old seedling alfalfa, grape, and pea were treated with 14C-labeled thifensulfuron, chlorsulfuron, glyphosate, 2,4-D, and bromoxynil. Significant amounts of all herbicides were absorbed by pea, alfalfa, and grape from the aqueous solutions, whereas very limited absorption occurred from herbicide-treated soil. Prolonged and multiple exposure to herbicide-treated soil did not increase herbicide uptake. High relative humidity enhanced herbicide absorption from aqueous solutions but not from herbicide-treated soil. All herbicides except bromoxynil were readily translocated in alfalfa, grape, and pea. Limited quantities of herbicides were absorbed from herbicide-treated soil by plant foliage, and this small amount is unlikely to cause crop damage.