Hostname: page-component-78c5997874-8bhkd Total loading time: 0 Render date: 2024-11-02T16:12:25.094Z Has data issue: false hasContentIssue false
  • English
  • Français

Detection of Herbicides Using Fluorescence Spectroscopy

Published online by Cambridge University Press:  12 June 2017

Thomas C. Mueller ,
Thomas B. Moorman  and
Martin A. Locke
Thomas C. Mueller
Affiliation:
USDA-ARS, South. Weed Sci. Lab. Stoneville, MS 38776
Thomas B. Moorman
Affiliation:
USDA-ARS, South. Weed Sci. Lab. Stoneville, MS 38776
Martin A. Locke
Affiliation:
USDA-ARS, South. Weed Sci. Lab. Stoneville, MS 38776
Get access Rights & Permissions [Opens in a new window]

Abstract

Fluorescence spectroscopy offers several advantages over other methods of detection and quantitation of chemical compounds. This technique has been underutilized in detection and quantitation of herbicides. The fluorescence properties of 39 herbicides representing several major types of chemistry were determined. The fluorescence of analytical standards was measured in acetonitrile, acetonitrile + water, and acetonitrile + water + strong acid. Fourteen of the 39 herbicides fluoresced to some extent, and seven (bentazon, chloramben, difenzoquat, fluometuron, imazaquin, MCPA, and norflurazon) were identified as good candidates for further method development. The technical advantages and disadvantages of fluorescence spectroscopy of herbicides are discussed.

Keywords

Bentazon, 3-(1-methylethyl)-(1H-2,1,3-benzothiadiazin-4(3H)-one 2,2-dioxidechloramben, 3-amino-2,5-dichlorobenzoic aciddifenzoquat, 1,2-dimethyl-3,5-diphenyl-1H-pyrazoliumfluometuron, N,N-dimethyl-N′-[3-(trifluoromethyl)phenyl]ureaimazaquin, 2-[4,5-dihydro-4-methyl-4-(1-methylethyl)-5-oxo-1H-imidazol-2-yl]-3-quinolinecarboxylic acidMCPA, (4-chloro-2-methylphenoxy) acetic acidnorflurazon, 4-chloro-5-(methylamino)-2-(3-(trifluoromethyl)phenyl)-3(2H)-pyridazinoneChemical analysisherbicide detectionHPLCpesticidefluorescence detection
Type
Soil, Air, and Water
Information
Weed Science , Volume 40 , Issue 2 , June 1992 , pp. 270 - 274
Copyright
Copyright © 1992 by the Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

1. Dornseiffen, J. W. and Verwaal, W. 1985. Determination of asulam residues in spinach by HPLC after application of asulam against weeds. Int. Symp. on Crop Prot. Conf. 47:867–876.Google Scholar
2. Duke, S. O., Duke, M. V., Sherman, T. D., and Nandihalli, U. B. Spectrophotometric and Spectrofluorometric Methods in Weed Science. Weed Sci. 39:505–513.Google Scholar
3. Garcia-Sanchez, F. and Cruces-Blanco, C. 1988. Spectrofluorometric determination of pesticide residue mixtures by isodifferential derivative spectroscopy. Anal. Chem. 60:323–328.Google Scholar
4. Goewie, C. E. and Hogendoorn, E. A. 1987. Pre-column clean-up and liquid chromatographic determination of residues of N-methylcarbamate pesticides in extracts of total diet. J. Chromatogr. 404:352–358.Google Scholar
5. Miano, T. M., Sposito, G., and Martin, J. P. 1988. Fluorescence spectroscopy of humic substances. Soil Sci. Soc. Am. J. 52:1016–1019.Google Scholar
6. Miles, C. J., Wallace, L. R., and Moye, H. A. 1986. Determination of glyphosate herbicide and (aminomethyl)phosphonic acid in natural waters by liquid chromatography using pre-column fluoregenic labeling with 9-fluorenylmethyl chloroformate. J. Assoc. Off. Anal. Chem. 69:458–461.Google Scholar
7. Mueller, T. C. and Moorman, T. B. 1991. Liquid chromatographic determination of fluometuron and metabolites in soil. J. Assoc. Off. Anal. Chem. 74:671–673.Google Scholar
8. Schüssler, W. 1990. Automatic measurement of bentazone and phenoxy acid herbicides by HPLC with three different detectors. Chromatographia 29:24–30.Google Scholar
9. Shaw, D. R., Peeper, T. F., and Nofziger, D. L. 1986. Evaluation of chlorophyll fluorescence parameters for an intact-plant herbicide bioassay. Crop Sci. 26:756–760.CrossRefGoogle Scholar
10. Shaw, D. R., Peeper, T. F., and Nofziger, D. L. 1985. Comparison of chlorophyll fluorescence and fresh weight as herbicide bioassay techniques. Weed Sci. 33:29–33.Google Scholar
11. Snyder, L. R. and Kirkland, J. J. 1979. Fluorometers. Pages 145–147 in Snyder, L. R. and Kirkland, J. J. Introduction to Modern Liquid Chromatography. John Wiley and Sons, New York.Google Scholar
12. Snyder, L. R. and Kirkland, J. J. 1979. Derivatizing agents. Pages 733–742 in Snyder, L. R. and Kirkland, J. J. Introduction to Modern Liquid Chromatography. John Wiley and Sons, New York.Google Scholar
13. Spittler, T. D., Marafioti, R. A., Helfman, G. W., and Morse, R. A. 1986. Determination of carbaryl in honeybees and pollen by high-performance liquid chromatography. J. Chromatogr. 352:439–443.Google Scholar