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Variation in ‘target’ antigens between appropriate and inappropriate hosts of Taenia saginata metacestodes

Published online by Cambridge University Press:  23 August 2011

L. J. S. Harrison ,
R. M. E. Parkhouse  and
M. M. H. Sewell
L. J. S. Harrison
Affiliation:
University of Edinburgh, Centre for Tropical Veterinary Medicine, Roslin, Midlothian EH 25 9RG
R. M. E. Parkhouse
Affiliation:
National Institute for Medical Research, Mill Hill, London NW7 1AA
M. M. H. Sewell
Affiliation:
University of Edinburgh, Centre for Tropical Veterinary Medicine, Roslin, Midlothian EH 25 9RG
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Summary

The antibody response of the appropriate hosts (cattle) to Taenia saginata larvae was compared with that of an inappropriate host (Balb/c mice) using gel electrophoresis followed by immunoelectrotransfer blot techniques (Western blotting). Three groups of cattle were included, those known to be resistant to challenge infection because of repeated oral challenge with T. saginata eggs over a 1-year period, a previously infected but known susceptible group and a group of uninfected controls. Serum from the mice and the two groups of infected cattle contained antibodies recognizing different ‘target’ antigens, some of which may be related to host resistance. The potential value of the technique of Western blotting in dissecting the humoral response of a particular host species to parasitic infection and in the identification of those antigens suitable for the production of effective vaccines is discussed.

Type
Research Article
Information
Parasitology , Volume 88 , Issue 4: Symposia of the British Society for Parasitology Volume 21: Parasite evasion of the immune response , August 1984 , pp. 659 - 663
Copyright
Copyright © Cambridge University Press 1984

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References

REFERENCES

Gallie, G. J. & Sewell, M. M. H. (1970). A technique for hatching Taenia saginata eggs. Veterinary Record 86, 749.CrossRefGoogle ScholarPubMed
Gallie, G. J. & Sewell, M. M. H. (1972). The survival of Cysticercus bovis in resistant calves. Veterinary Record 91, 481–2.CrossRefGoogle ScholarPubMed
Gallie, G. J. & Sewell, M. M. H. (1974). The serological response of calves infected neonatally with Taenia saginata (Cysticercus bovis). Tropical Animal Health and Production 6, 173–7.CrossRefGoogle Scholar
Geerts, S., Kumar, V. & Brandt, J. R. A. (1982). A preliminary report on Meriones unguiculatus as an experimental host for Taenia saginata metacestodes. Veterinary Research Communications 5, 301–4.CrossRefGoogle ScholarPubMed
Harrison, L. J. S. & Sewell, M. M. H. (1981). A comparison of the enzyme-linked immuno-sorbent assay and the indirect haemagglutination technique applied to sera from cattle experimentally infected with Taenia saginata (Goeze, 1782). Veterinary Immunology and Immunopathology 2, 67–73.CrossRefGoogle Scholar
Mitchell, G. F., Anders, R. F., Brown, G. V., Handeman, E., Roberts-Thomson, I. C., Chapman, C. B., Forsyth, K. P., Kahl, L. P. & Cruise, K. M. (1982). Analysis of infection characteristics and antiparasite immune responses in resistant compared with susceptible hosts. Immunological Reviews 61, 137–88.CrossRefGoogle ScholarPubMed
Rajaskariah, G. R. & Rickard, M. D. (1980). Density gradient separation of Taenia pisiformis oncospheres. Journal of Parasitology 66, 355–6.CrossRefGoogle Scholar
Rickard, M. D. & Williams, J. F. (1982). Hydatidosis/cysticercosis: immune mechanisms and immunization against infection. Advances in Parasitology 21, 230–96.Google ScholarPubMed
Soulsby, E. J. L. & Lloyd, S. (1982). Passive immunization in cysticercosis: characterization of antibodies concerned. In Cysticercosis – Present State of Knowledge and Perspectives, (ed. Flisser, A.Willms, K.Laclette, J. P.Larralde, C.Ridaura, C. and Beltran, F.), pp. 539–574. New York and London: Academic Press.Google Scholar
Towbin, H., Staehelin, T. & Gordon, J. (1979). Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Science USA 76, 4350–4.CrossRefGoogle ScholarPubMed
Tsang, V. C. W., Peralta, J. M. & Simmons, A. R. (1983). Enzyme-linked immunoelectro-transfer blot techniques (EITB) for studying the specificities of antigens and antibodies separated by gel electrophoresis. Methods in Enzymology 92, 377–91.CrossRefGoogle Scholar