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Development of a new set of phages as an epidemiological marker in Staphylococcus epidermidis causing nosocomial infections

Published online by Cambridge University Press:  15 May 2009

M. M. Martίn-De-Nicolás ,
A. Vindel  and
J. A. Sáez-Nieto
M. M. Martίn-De-Nicolás
Affiliation:
Laboratorio de Referenda de fagotipia de Staphylococcus aureus, Servicio de Bacteriología, Centro National de Microbiología, Virología e Inmunología, Sanitarias, 28220 Majadahonda, Madrid, Spain.
A. Vindel
Affiliation:
Laboratorio de Referenda de fagotipia de Staphylococcus aureus, Servicio de Bacteriología, Centro National de Microbiología, Virología e Inmunología, Sanitarias, 28220 Majadahonda, Madrid, Spain.
J. A. Sáez-Nieto*
Affiliation:
Laboratorio de Referenda de fagotipia de Staphylococcus aureus, Servicio de Bacteriología, Centro National de Microbiología, Virología e Inmunología, Sanitarias, 28220 Majadahonda, Madrid, Spain.
*
*Author for correspondence.
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Summary

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We describe the isolation of a new set of phages for typing Staphylococcus epidermidis. One hundred and eighty-two phages were obtained from S. epidermidis strains of human origin. Twelve phages were selected according to their potency and their lytic activity studied. Twenty phages of the Dean and Williams' set were also studied.

Phage-typing was undertaken at 100 × RTD, 1000 × RTD and after heat treatment at 48 °C. When the two sets of phages were compared separately similar figures were obtained. When the two typing sets wrere combined, the percentage of typability for the 182 bacterial strains increased to 29⋅1% using 1000 x RTD and to 75⋅3% after heat treatment.

Type
Research Article
Information
Epidemiology & Infection , Volume 104 , Issue 1 , February 1990 , pp. 111 - 118
Copyright
Copyright © Cambridge University Press 1990

References

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