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Development of a new set of phages as an epidemiological marker in Staphylococcus epidermidis causing nosocomial infections

Published online by Cambridge University Press:  15 May 2009

M. M. Martίn-De-Nicolás ,
A. Vindel  and
J. A. Sáez-Nieto
M. M. Martίn-De-Nicolás
Affiliation:
Laboratorio de Referenda de fagotipia de Staphylococcus aureus, Servicio de Bacteriología, Centro National de Microbiología, Virología e Inmunología, Sanitarias, 28220 Majadahonda, Madrid, Spain.
A. Vindel
Affiliation:
Laboratorio de Referenda de fagotipia de Staphylococcus aureus, Servicio de Bacteriología, Centro National de Microbiología, Virología e Inmunología, Sanitarias, 28220 Majadahonda, Madrid, Spain.
J. A. Sáez-Nieto*
Affiliation:
Laboratorio de Referenda de fagotipia de Staphylococcus aureus, Servicio de Bacteriología, Centro National de Microbiología, Virología e Inmunología, Sanitarias, 28220 Majadahonda, Madrid, Spain.
*
*Author for correspondence.
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Summary

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We describe the isolation of a new set of phages for typing Staphylococcus epidermidis. One hundred and eighty-two phages were obtained from S. epidermidis strains of human origin. Twelve phages were selected according to their potency and their lytic activity studied. Twenty phages of the Dean and Williams' set were also studied.

Phage-typing was undertaken at 100 × RTD, 1000 × RTD and after heat treatment at 48 °C. When the two sets of phages were compared separately similar figures were obtained. When the two typing sets wrere combined, the percentage of typability for the 182 bacterial strains increased to 29⋅1% using 1000 x RTD and to 75⋅3% after heat treatment.

Type
Research Article
Information
Epidemiology & Infection , Volume 104 , Issue 1 , February 1990 , pp. 111 - 118
Copyright
Copyright © Cambridge University Press 1990

References

REFERENCES

1.Price, EH.Staphylococcus epidermidis infections of eerebrospinal fluid shunts. J Hosp Infect 1984; 5: 7–17.CrossRefGoogle ScholarPubMed
2.Parker, MT. Session on application of phage-typing to the epidemiology and ecology of coagulase-negative staphyloeoeei. In: Pulverer, G.Hecko, PB.Peters, G, eds. Phage-typing of coagulase-negative staphyloeoeei. Stuttgart: Fisher-Verlag, 1979: 165–7.Google Scholar
3.Kloos, WE, Jorgensen, JH. Staphyloeoeei. In: Lennette, EH, Balows, A, Hausler, WJ, Shadomy, JH, eds. Manual of Clinical Microbiology. 4th ed.Washington: American Society for Microbiology, 1985: 143–53.Google Scholar
4.Kloos, WE.Coagulase-negative staphyloeoeei. Clin Microbiol Newsl 1982; 4: 75–9.CrossRefGoogle Scholar
5.Kloos, WE, Sehleifer, KH.Simplified scheme for routine identification of human Staphylococcus species. J. Clin Microbiol 1975; 1: 82–8.CrossRefGoogle ScholarPubMed
6.De Saxe, MS, Notley, CM. Experiences with typing of coagulase negative staphyloeoeei and mierococci. Tn: Pulverer, G, Hecko, PB, Peters, G, eds. Phage typing of coagulase-negative staphyloeoeei. Stuttgart: Fisher-Verlag, 1979: 46–59.Google Scholar
7.Blair, JE, Williams, REO.Phage typing of staphyloeoeei. Bull WHO 1961; 24: 771–84.Google Scholar
8.Dean, BA, Williams, REO.Hall, F.Corse, J.Phage typing of coagulase-negative staphyloeoeei and microeoeci. J Hyg 1973; 71: 261–70.CrossRefGoogle Scholar
9.Verhoef, J, VanBoven, CPA.Winkler, KC.Characters of phages from coagulase-negative staphyloeoeei. J Med Microbiol 1971, 4: 413–24.CrossRefGoogle Scholar
10.Dowsett, EG.Petts, DX.Baker, SL, et al. , Analysis of an outbreak of staphylococcal scalded skin syndrome. J Hosp Infect 1984; 5: 391–7.CrossRefGoogle ScholarPubMed
11.Vindel, A.Martín-Bourgon, C, Sáez-Nieto, JA.Characterization of non-typable strains of Staphylococcus aureun from cases of hospital infection.Epidemiol Infect 1987; 99: 191–200.CrossRefGoogle ScholarPubMed
12.Lacey, RW, Keyword, X.Lincoln, C.Staphvlococci in the UK: a review. J Antimierob Cheniother 1984; 14 Suppl D: 19–25.Google ScholarPubMed
13.Lorian, V, Zak, O, Suter, J, Breucher, C.Staphylococci ‘in vivo’ and ‘in vitro’. Diag Microbiol Infect Dis 1985; 3: 433–44.CrossRefGoogle Scholar
14.Sompolinsky, D, Samra, S, Karakawa, WW, et al. , Encapsulation and capsular types in isolates of S. aureus from different sources and relationship to phage types. J Clin Microbiol 1985; 22: 828–34.CrossRefGoogle ScholarPubMed
15.Parisi, JT, Talbot, HW, Shakan, JM. Development of Phage-Typing Set for Staphylococcus epidermidis in the United States. In: Pulverer, G, Hecko, PB, Peters, G, eds. Phage-typing of coagulase-negative staphylococci. Stuttgart: Fisher–Verlag, 1979: 60–7.Google Scholar
16.Schmidt, WC, Jeffries, CD.Bacteriophage typing of gram negative rod–shaped bacteria. CRC Crit Rev Clin Lab Sci 1975; 6: 201–46.CrossRefGoogle ScholarPubMed
17.Richardson, JF, Marples, RR. Phage typability of coagulase–negative staphylococci in relation to antibiotic resistance. In: Pulverer, G, Quie, PG, Peters, G, eds. Pathogenicity and clinical significance of coagulase-negative staphylococci. Stuttgart: Fisher–Verlag, 1987: 235–46.Google Scholar