In view of the sporadic nature of outbreaks of Gryllus bimaculatus Deg., all stages of this cricket were reared in Israel at various temperatures in order to find the effect on its development and fecundity. Eggs were incubated at constant temperatures and also at fluctuating room temperatures. The mean incubation period varied from 7·5 days at 34°C. to 75 days at a mean of 16·4°C.; at 26°C., at which the percentage hatching was greatest, it was 14 days; and the threshold of development was calculated to be about 15°C. For larvae reared at constant temperatures on a diet of bread and fishmeal, the development period varied from about 32 days at 34°C. to 130 days at 22°C.; the threshold was calculated to be 19°C. For adults maintained at constant temperatures, the mean life at temperatures of 26 to 34°C. usually exceeded one month, the preoviposition period varied from 4 days at 34°C. to about 15 at 22°C., and the threshold of reproduction was calculated as 19°C. Of 16 females tested that had been reared and maintained at near optimal temperatures (30–34°C.), more than half gave rise to upwards of 500 larvae each, and two to over 3,000 each. The reproductive capacity was affected by the temperature at which the larva was reared, the number of offspring produced being greatest from pairs of adults reared at 26°, and nil from females reared at 37°C. Light-trap catches during three years showed a peak in midsummer or autumn.

Of 26 amino-acids tested against a solvent control for their attractiveness to females of Aedes aegypti (L.), lysine was the most attractive and six others (threonine, 1-methylhistidine, arginine, tyrosine, glutamine and alanine) were significantly attractive; nine amino-acids were significantly repellent. Lysine converted to the monopicrate, then to the dihydrochloride and then back to the free base was attractive. The degree of attractiveness of lysine was in direct proportion to the amount of uncombined CO2 it inevitably adsorbs. It is also proportional to the amount of carbamino CO2 combined in the molecule. Some commercial samples of lysine contain repellent principles removable by distillation. It is concluded that the attractiveness of lysine to A. aegypti is due to its ability to act as a vehicle for CO2, both adsorbed and in carbamino combination.

Fifty-two standardised weekly collections of female mosquitos were made by human catchers at ground-level in lake-side forest near Entebbe, Uganda. The numbers of nullipars and pars in each sample were recorded, in order to determine whether the capacity of these mosquito populations to transmit virus varied seasonally. Methods are described for handling large catches for the purpose of identifying and age-grading. The total sample comprised 31,978 mosquitos belonging to 37 or more species. Results for 15 species are presented in detail.

The nulliparous rate (i.e., the number of nullipars as a percentage of total females) differs markedly between species, but relatively little within species. Certain species of Mansonia (Coquillettidia), mainly swamp breeders, have the highest nulliparous rate, and species of Aedes and Eretmapodites, which breed in small containers, have the lowest. In some species, biting females show a lower nulliparous rate than those caught resting.

Culex annulioris Theo. and species of Mansonia, which are swamp breeders, have a main peak of abundance between May and July, during the main rains and when lake-level is highest. Species of Aedes (Stegomyia), which are tree-hole or small-container breeders, show small, irregular, short-term fluctuations in numbers.

The number of pars, an epidemiologically important parameter, is absolutely highest at or soon after the time of greatest abundance. For certain bird-biting mosquitos, namely, Culex annulioris and species of Mansonia (Coquillettidia), most pars are found during the main rains, when susceptible hosts are also most numerous. Implications of these findings on virus-isolation programmes are briefly discussed.

Series of 24-hour catches at different levels were made at Zika and Lunyo in the Entebbe area, Uganda, using fowls (White Leghorn cocks) as bait, and some comparative catches were made with man as bait.

Thirty-seven different mosquito species (including five species groups) were taken on the avian bait.

The vertical distributions and biting cycles of each of nine species (including one species group), Anopheles implexus (Theo.), Mansonia (Coquillettidia) aurites (Theo.) gp., M. (C.) fuscopennata (Theo.), M. (Mansonioides) africana (Theo.), Hodgesia cyptopus Theo., Aedes africanus (Theo.), A. apicoargenteus (Theo.), A. ingrami Edw. and Culex annulioris Theo. were similar on human and avian bait.

At Lunyo both A. africanus and C. annulioris had a marked diurnal peak, which had not been described previously.

Comparison of the numbers coming to avian and human bait indicate that species of Culex, H. cyptopus and most of the species of Mansonia (Coquillettidia) have a preference for birds.

Decorticated groundnuts (Arachis) are stored in bags in pyramid-shaped stacks in Northern Nigeria over the period November to October. In Kano the dry season extends from October until March and is followed by a short hot season preceding the rainy season, which usually commences during May.

The seasonal distribution within such pyramids of the various insect species infesting groundnuts is considered in some detail, with particular reference to the primary pest Tribolium castaneum (Hbst.). This distribution is discussed in relation to temperature and moisture content of the kernels and to the effect on the environment of covering pyramids with tarpaulins, the normal practice for protection during the rainy season. T. castaneum is the only species present on groundnuts initially and throughout the storage season. It is concluded that the elimination, during storage, of other species also present initially, i.e., Cadra cautella (Wlk.), Plodia interpunctella (Hb.), Corcyra cephalonica (Stnt.) and Oryzaephihis mercator (Fauv.), and the ultimate concentration of infestation by T. castaneum within peripheral bags is the result of changes in physical conditions. It would appear that Cryptolestes ferrugineus (Steph.), which was not found until late in the storage season, is a pest of secondary importance.

The significance of the results obtained in relation to control measures is discussed, bearing in mind that all but one of the pests considered were found to be present on groundnuts when received at the Kano central storage area from buying stations. It is concluded that a programme of surface spraying of pyramids with insecticides is of little value and recommendations for the introduction of initial fumigation of pyramids have been put forward.

Brief notes are given on the distribution and habits of 42 species of Scolytidae and 34 of Platypodidae that occur as common forest insects in Ghana. A considerable number of these can be considered as beneficial insects because they do little damage but assist in the rapid break-down of dead trees and felling slash. Others are injurious mainly as timber borers, shoot borers or, generally to a lesser extent, as bark beetles and seed insects. Trachyostus ghanaensis Schedl and Doliopygus dubius (Samps.) are of outstanding importance in their constant or frequent habit of attacking the wood of living trees of commercial value; Platypus hintzi Schanf., Doliopygus conradti (Strohm.) and D. brevis (Strohm.) are probably the most important ambrosia beetle borers of newly felled timber; and Xyleborus semiopacus Eichh. has been known to destroy whole plantations of young forest trees.

Ten nominal species of DELPHACIDAE have hitherto been regarded as synonymous with Sogata, furcifera (Horv.), which is notorious as a pest of Graminaceous crops in eastern Asia. In a recent study it was established that two members of this complex represented distinct species, and it was accordingly considered necessary to re-examine the status of the remainder: the results of such a study are reported in this paper. The members of this complex now recognised as distinct species number more than twenty: some are restored from synonymy and others are described as new, and all, as appropriate, are referred to one of three genera, Matutinus Distant, Sogatella Fennah (here accorded generic status) and a new genus, Sogatodes. Most of the species in the complex are redescribed and a key is given for the separation of all.

The effect of DDT applied to cotton plants four times at 10-day intervals at a rate of 1 lb. active ingredient per acre per application against Hemitarsonemus latus Banks was studied at Serere Eesearch Station, Uganda. DDT applied as a 10 per cent, dust, or in a spray from a 50 per cent, wettable powder increased the damage caused by the mite, while DDT applied in a spray from a 25 per cent. miscible liquid decreased it.

Another trial using a DDT miscible-liquid spray applied at intervals of 10, 15 and 20 days at 1 lb. active ingredient per acre per application demonstrated partial control of the mite. However, when spraying ceased, tea-mite damage increased. At the final count all spray intervals showed significantly less damage than the control and there was an over-all significant difference between spray treatments and the control. In both trials the number of mite-infested plants increased in an exponential manner with time.

It is not considered that the present recommended practice of applying DDT four times, at 15-day intervals, in a spray from 25 per cent, miscible liquid at 1 lb. active ingredient per acre will cause an increase of the mite on peasant-grown cotton in Uganda, although it will only partially control any infestation of it that is present.

Dispersion behaviour of the adult of the lesser grain borer, Rhyzopertlia dominica (F.), was investigated under controlled conditions by observing the numbers occurring on the surface, or at various depths below it, in small bulks of grain. The effects of population density, temperature and moisture content of the grain on numbers at the surface were studied by using groups of insects in 500 g. wheat, and on dispersion in the vertical plane by confining beetles within a monogranular plate of wheat held between vertical glass sheets, through which the positions of individuals could be observed.

In grain at 25°C. and 14 per cent, moisture content (70 per cent. R.H.), the proportion of a group on the surface averaged approximately six per cent, for densities of 5–100 insects in 500 g., and the proportion was higher than in grain at the same temperature and 9 or 17 per cent, moisture, or at the same moisture content and 15 or 35°C.

In grain of 14 per cent, moisture content, the level of individual locomotory activity increased from 15 to 35°C. At 25°C., locomotory activity was depressed in grain of 9 and 14 as compared with 17 per cent, moisture content. These results are discussed in relation to group formation and dispersal of R. dominica and its contact with Sitophilus granarius (L.) if both species are present in the same grain bulk.

Several species of Dynastid beetles are serious pests of yam (Dioscorea) in Nigeria, the adults attacking the setts and new tubers underground. The base of the yam vine and the roots are not generally damaged. The most important species is Heteroligus meles (Billb.).

H. meles does not breed in the yam fields, and the adults fly to them from breeding grounds that are distinct, and to which they return later.

The cultivation and growth of the yam plant in Nigeria are briefly described. Small tubers, or cut pieces of tubers (setts) are planted. The earlier the date of planting and of emergence of the vine from the soil, the heavier the vine and the new tuber.

An account is given of observations on the infestation, in relation to crop growth, of a plot of yellow yams (D. cayenensis) planted near Benin, in Western Nigeria on 29th March 1958.

Adults of H. meles were taken, from 3rd April to 26th June and again from 8th to 21st November, between 7 and 9 p.m., at a light-trap placed at a distance of ¼ mile from the plot.

Beetles entered the soil of yam hills within six inches of the vine, usually before 7 a.m., on mornings following evening flights, the entry holes usually being identifiable. The first vines were seen in the experimental plot on 19th April. Beetles did not enter a yam hill unless the vine had emerged.

The longer the exposure to flying beetles, the more likely were plants to be attacked, and bigger vines attracted more beetles than did smaller ones. Later planting decreased beetle damage, but a lower yield resulted.

At first the beetles fed on the sett, making and increasing the size of fewer lesions than later on the new yam. About the time that the flying period ended, the setts were starting to rot, and the beetles left the immediate vicinity of the yam plant, as no acceptable food was available. Most moved deeper into the soil, but a few left the plant altogether and flew away. After the new tubers had begun to develop, the beetles returned in early August from their positions deeper in the soil to feed on them, constantly making new lesions, more numerous but smaller than those made in the setts, averaging about 4 g. of yam eaten.

During late August and again in late September, a few beetles moved underground from the original plants attacked to others. In mid-November, the beetles left the yam fields altogether, presumably returning to the breeding area.

The reasons why the beetles fly away from the yam fields remain uncertain, but they may include the seasonal decline in rainfall, the maturation of the yam tuber and the development of the gonads.

Factors that can affect the rate of attack are the dates of planting and harvesting in relation to the period during which the beetles fly, the method of cultivation and the variety of yam planted.

A technique is described for exposing insects to insecticidal deposits on detached foliage. Treated leaves are laid horizontally on boards with their petioles projecting through holes into water. Each insect is confined to the leaf surface by a small glass ring coated on its inner side with polytetrafluoroethylene and surrounded by a heavy metal ring to hold the leaf flat. This method is satisfactory for insects that do not spin webs and are unable to take off at a steep angle.

Populations of the wingless form of Myzus persicae (Sulz.), infected with severe yellows (SBYV), mild yellows (SBMYV), and both viruses, were introduced to single sugar-beet plants which were growing in a normal crop.

The aphids and their progeny walked readily from plant to plant, both along and across the rows, during the following eight weeks. Although they were heavily attacked by ladybirds, their mobility saved the population from total destruction. They carried and transmitted the viruses; within a month patches of sugar beet more than 11 feet in diameter had become infected, and within two months the diameter had extended to about 20 feet.

SBMYV spread to more than twice as many plants as SBYV. When introduced alone, either virus spread to twice as many plants as when the two viruses were introduced together. The rate of increase of the infection fell off during the season; it was estimated as eight- to sixteen-fold per month in June—July, about three-fold in August, and 1·5- to 1·9-fold in September. Natural infections-appear to behave similarly.

Apterae of M. persicae are considered to be predominantly responsible for yellows virus transmission within the crop, and their mobility, in contrast to the immobility of those of A. fabae Scop., is considered to be the principal reason why M. persicae is a much more important vector than A. fabae.

The extent of spread of virus by apterae shown to occur in the root crop must inevitably be much greater in the seed crop, in which aphid populations are much higher. This consideration serves to explain why existing control measures in the seed crop do not protect the root crop from infection. The risk from the seed crop, which is the reservoir of the disease, would be countered if seed crops were grown only in alternate years.

Experiments in south-east England are described in which insecticides were tested against the Carabid beetles Harpahis rufipcs (Deg.) and Feronia melanaria(111.), which cause damage to strawberry fruits.

In preliminary screening tests, the mortality of H. rufipex was low when adults were caged with fruits or straw treated with rotenone at 0·004 per cent, or with carbaryl, DDT, dimethoate, malathion, mevinphos, nicotine, TEPP, toxaphene and trichlorphon at one or more concentrations in the range 0–06–0–2 per cent. In small-scale field trials, γ BHC at 0·026 per cent, gave poor results but chlordane and dieldrin exhibited persistent toxicity.

In further tests with fruits, field-weathered deposits from sprays containing 0·1 or 0·2 per cent, dieldrin caused over 90 per cent, mortality for 7 and 21 days, respectively, but left residues at harvest of 0·5 p.p.m., which were considered too high for consumer safety.

Dieldrin applied to soil at 5 lb. per acre resulted in 100 per cent, mortality for 28 days in 1957, but gave very variable results in 1958. Conversely, aldrin at 5 lb. gave effective mortality for only 14 days in 1957 but for the maximum test period of 89 days in 1958; no satisfactory explanation was found for the seasonal differences. Aldrin at 10 lb. per acre gave 100 per cent, mortality for 77 and 89 days, respectively, in the two years. Tests of soils into which measured quantities of insecticide had been incorporated, resulted in 100 per cent, mortality, after 4 days' exposure, with aldrin and dieldrin at levels of 2–4 and 16–32 p.p.m., respectively; endrin at 64 p.p.m. was only slightly toxic.

In field trials in 1959–60, application of aldrin at 10 lb. per acre to the surface of the soil around the plants in sprays in late April immediately before flowering, or at 5 lb. per acre in late May before laying straw below the fruit, reduced fruit damage due to H. rufipes or F. melanaria by 66–94 per cent. Residues at harvest were of the order of 0·03 p.p.m., and no effect on flavour was detected in processed fruit from plots treated at either time of application.

Dispersion behaviour in adults of the saw-toothed grain beetle, Oryzaephilus surinamensis (L.), was studied under controlled laboratory conditions. The effect of density, temperature and moisture content on surface dispersion was studied using groups of insects in jars of 500 g. grain and vertical dispersion by using groups of insects in thin vertical plates of wheat held between two sheets of glass. The ethological basis of group behaviour was investigated by using isolated individuals.

Over the range of conditions used, 1–10 per cent, of the individuals comprising the groups studied appeared on the surface of the grain. In grain at 14 per cent, moisture content, at 25°C., 3·4 per cent, of the group appeared on the surface. A greater proportion was found on the surface of grain at 9 per cent, moisture content than at higher moisture contents at all temperatures tested (15–35°C.). At all moisture contents, surface numbers were relatively high at 15°C.

In grain at 14 per cent, moisture content and 25°C., individuals moved vertically and laterally at random but were more active in the evening than in the morning. In grain at 9 per cent, moisture, there was marked irritability, and at 15°C. and 14 per cent, moisture, there was continual but slow movement without any detectable diurnal pattern of activity. Activity was reduced at 30°C., and at 25°C. it was reduced in grain of 17 per cent, moisture content.

The significance of these results is discussed in relation to the detection of populations, group formation and the initiation of foci of heating in patches of damp grain.

The genetics of diazinon resistance in an Australian strain of Musca domestica L. was investigated by means of two series of repeated back-crosses of the hybrid resistant form to the susceptible. Resistance was maintained through nine and eleven generations in the separate studies. The ratio of susceptible to resistant hybrid flies found in the progeny of each cross was approximately 1:1.

In the second series, the frequency data were supported by dosage/response information on the relationship between dosage of insecticide and mortality. Together with the result of a previous study, the data furnish strong evidence that a fully dominant factor is responsible for the diazinon resistance in the Perth strain of house-flies resistant to organic phosphate insecticides.

A technique is described whereby a small bulk of grain can be broken down, all insects infesting it recovered and their positions recorded. A Perspex box, 12 inches cube, is loaded with 64 three-inch-cube bags of Terylene net (10 meshes to the inch) each holding 390 g. of Manitoba wheat, and the test insects (adult grain beetles) released on the surface, after which the box is sealed and left in darkness for the required time. The bags are then removed, the insects sieved out, and their numbers and positions recorded, thus enabling the pattern of group dispersion to be plotted. The net used did not appear seriously to impede the movement of the adults of Sitophilus granarius (L.), the largest species used in the study. Temperature gradients may be introduced and measured by thermocouples built into the bulk.

An operation is described in which approximately 100 sq. miles of heavily infested tsetse country in Southern Rhodesia, supporting both Glossina morsitans Westw. and G. pallidipes Aust. and an abundant and varied game population and formerly an area of endemic sleeping sickness attributable to Trypanosoma rhodesicnse, received six applications of 4 per cent, γ BHC in diesolene, discharged from aircraft in the form of a coarse aerosol, between May and September 1957. The area was a valley bounded by an escarpment, which provided conditions of relative isolation.

Fly-round catches of G. morsitans taken before, during and after the operation indicated that in those parts of the area that had received uniform treatment an estimated reduction in population, after the final application, of between 96·6 and 100 per cent, had been achieved, but where only the vegetation forming the riverine fringes had been treated the reduction was less. Quantitative data on the effect of the treatment on G. pallidipes were not collected, but the species ceased to be found in an investigation on sampling techniques that had been in progress in the area.

The area had been chosen for a resettlement scheme of indigenous people that was due to begin before October 1957, and the purpose of the spraying operation was to reduce the population of tsetses to a level that would permit the introduction of the people and their stock with comparative safety from trypanosomiasis. It was hoped that the number of people to be resettled would be sufficient to clear most of the tsetse habitat in their text-abstract agricultural activities and that the tsetses would be permanently excluded from the valley. Four to five years after the start of resettlement there were still several uncleared areas remaining, but the situation was continually improving, and estimates in 1962 indicated that the population of G. morsitans had remained at a low level, showing a reduction of between 81 and 100 per cent, as compared with that prevailing before the spraying operation. The stock introduced in 1957 were maintained under drug treatment, and the rate of infection with T. congolense was kept below a 3 per cent, level; no cattle died from trypanosomiasis, and their numbers had more than doubled by 1962. No case of sleeping sickness occurred.

The work described here showed that, under conditions of relative isolation, G. morsitans can be sufficiently reduced in numbers by means of an insecticidal aerosol discharged from aircraft to permit the successful introduction of people and stock, provided that adequate veterinary supervision is given, and that under these conditions settlement can prevent the recovery of a population of this species.

The dispersion behaviour of Tribolium castaneum (Hbst.) and Cryptolestes ferrugineus (Steph.) was studied under controlled laboratory conditions. The effect of density, temperature and moisture content of grain on surface numbers was studied using groups of insects initially placed centrally in jars containing 500 g. wheat and vertical dispersion was studied by observing groups of insects in a thin plate of grain held vertically between two glass sheets. The underlying behaviour of group dispersion was studied, using isolated individuals. Over the range of conditions used (15–35°C, and 9–17 per cent. moisture content), 14–49 per cent. of groups of Tribolium and 0·2–4·0 per cent. of Cryptolestes appeared on the surface. Surface numbers in both species were depressed at each end of the temperature range. In the middle of the temperature range, more individuals of Tribolium were found on the surface in grain at 17 per cent. moisture content than at 9 per cent., the reverse being found with Cryptolestes.

Analyses of locomotory activity in Tribolium showed that in all conditions females moved around less than males. For both sexes, activity was relatively depressed in the driest grain and at the lowest temperatures. Change in position within the grain was almost totally absent at 35°C. although locomotory activity remained relatively high. In Cryptolestes, locomotory activity was likewise depressed at the lowest temperature but both sexes moved around apparently at random in grain at 14 per cent. moisture and 30°C. In grain at 9 per cent. moisture, nearly all individuals became inactive within 24 hours. These results are discussed in relation to the onset and detection of infestation in grain and to the associations of grain-infesting species.

Observations were made in Fiji on the ecology and control of two Delphacid planthoppers on rice, Sogatella furcifera (Horv.) and Nilaparvata lugens (Stål), the latter species having apparently been responsible for a devastating outbreak in 1959. The studies covered three rice seasons, 1961, 1962, and 1963, most of the research being done at Koronivia in the south-eastern part of Viti Levu. Samples were taken of planthoppers and their natural enemies in dryland (drilled) and wetland (transplanted) rice, as well as in rice seed-beds and areas with grass or mixed rice and grass. Insecticides applied at standard dosages were tested against the planthoppers.

It was shown that S. furcifera was most common on young rice and that N. lugens predominated on older rice. Sogatella kolophon (Kirk.), long placed in the genus Sogata and confused with Sogatella furcifera, was abundant only in grassy areas. Populations of N. lugens and, to a lesser extent, of S. furcifera, were apt to reach damaging levels on transplanted rice growing in pools of standing water after a dry period. In drilled fields, the predatory Mirid Cyrtorhinus lividipennis vitiensis Usinger provided effective control by destroying eggs of N. lugens, and, by the time the rice was six months old, it usually outnumbered the females of N. lugens. Parasitism of planthopper eggs by Mymarids, Trichogrammatids, and Eulophids was very low. Parasitism of adults of S. furcifera and S. kolophon by Elenchid Strepsipterons averaged about ten per cent, and that by Dryinid wasps less than one per cent. Adults of N. lugens were virtually free from parasitism but were sometimes infected by fungi. Factors influencing outbreaks are discussed. These include the amount of rainfall, the type of cultivation, the age of the rice crop, the species composition of the planthopper populations, and the relative abundance of Cyrtorhinus.

Insecticidal concentrates tested against both S. furcifera and N. lugens included malathion 50 per cent, emulsifiable concentrate, diazinon 20 per cent, e.c., Bogor 30 per cent, e.c., DDT 20 per cent, e.c., dieldrin 15 per cent, e.c., and Sevin 80 per cent, wettable powder. Dieldrin-resistant strains of N. lugens appeared during 1961, the third season in which dieldrin and BHC had been widely used. The other five materials gave good results, DDT being the least expensive and the most persistent, although it took more than 24 hours to reduce the plant-hopper populations. It was concluded that rice should be closely watched from its third to the end of its sixth month and that 50 oz. of DDT 20 per cent. e.c. in 50 gallons of water per acre, or an equivalent formulation of some other insecticide, should be sprayed on the rice stems if the planthopper density should exceed 10 per stem. Other possible measures for controlling rice planthoppers would include the introduction of Dryinid wasps parasitising N. lugens, the development of rice varieties resistant to planthopper attacks, and the use of controlled flooding in high-bunded fields, but on present evidence the first two appear to hold little promise of success.

Glossina, palpalis (R.-D.) was studied in three localities near Voinjama, in a forest region in north-west Liberia, where human trypanosomiasis persists only as sporadic cases. Sites were chosen with different degrees of contact between man, fly and game, in transitional vegetation on the Zeliba River, and in primary forest on the Lofa and Lawa Rivers. The general features, fauna and climate of these areas are described. Catching by nets was found to be preferable to the use of traps. Trap catches averaged about one-quarter of the corresponding net catches. The theoretical basis of ‘animal’ traps is too uncertain for their use in quantitative studies, and under forest conditions there are practical difficulties in siting them in optimum positions; they failed to catch at all at very low fly densities. Heavy rain and the dry season were unfavourable to the fly, particularly outside the forest canopy. A wet-season resting site on horizontal twigs near to the ground is described. The percentage of females in the total catch differed significantly between different habitats, but apparently was not indicative of the degree of hunger in the population. There were no great differences between the sexes in activity patterns, and activity was greatest during the apparently unfavourable climatic conditions of early afternoon. The willingness of the fly to penetrate unfavourable surroundings suggests that the clearing of forest at potential places of transmission is unlikely to afford an effective means of protection. There is some evidence that breeding was curtailed during the rains. Very few engorged flies were found. Fly numbers and distribution were not affected by normal game movements. There is no evidence that the fly was ever dependent on man for food. Where game was driven away the fly tended to follow it. Occasional human foci did not affect the number or distribution of the fly, and fly activity was not correlated with human activity. Intimate and prolonged contact between man and fly did not occur.