We examined the effects of daily positive or negative human handling on the behaviour of Holstein-Friesian dairy calves (n = 20 calves per treatment, five calves per group). The response to humans and indicators of positive emotions were examined at four weeks of age. Calves that received positive handling approached a familiar handler within 1 min in 50% of the handling sessions compared to 17% of the sessions for negatively handled calves but showed no difference when approaching an unfamiliar person. Calves that received positive handling showed less avoidance behaviour in their home pen to an approaching unfamiliar person (score, positive: 3.7, negative: 2.8) but there was no treatment effect on flight distance when tested outside the home pen. Both treatment groups responded similarly to a novel object and performed the same amount of play behaviour. Calves that received positive handling interacted more with cow brushes than calves that received negative handling (positive: 9.9%, negative: 7.9% of the total time). At three months of age, avoidance behaviour was re-tested, this time including 20 control animals of the same breed and age, reared routinely on-farm. Controls showed more avoidance behaviour (positive: 1.5, negative: 1.0, control: 0.3) and had a greater flight distance (positive: 3.3 m, negative: 3.7 m, control: 4.9 m). The results confirm existing literature demonstrating that the quantity and quality of handling influence the response towards humans. Little evidence was found that the type of early handling influences behaviours indicative of positive emotions.

Arguably, grounding animal ethics in traditional moral theories such as utilitarianism or rights-based ethics is impoverished since they emphasise impartiality and abstractness in our ethical deliberations at the expense of giving proper weight to special relationships we have with other individuals. Here, I explore the human-animal bond as a starting point for animal ethics, and focus on the resulting moral implications of this bond on farm animal welfare. The human-animal bond revisits values inherent in the nature of animal husbandry and is also influenced by philosophical ethics of caring. Farmers or stockpersons who form close bonds with their animals make an implicit promise to discharge duties to their animal companions above and beyond respectful treatment as sentient beings. Scientific study suggests that interpersonal human-animal relationships may translate to better care and consideration for farmed animals, promoting both better animal welfare and on-farm productivity. Acknowledging the existence of human-animal bonds on the farm and encouraging farmers and animal handlers not to shy away from forming bonds with their animals is recommended. Farmers, stockpersons, and contract-farmers for agribusinesses should be given an ethical voice to lodge grievances about how farmed animals are treated and be encouraged to participate in discussions on farming practices and animal welfare standards. They should also be educated on gains made through scientific enquiry regarding the capacities and needs of animals as well as on welfare advances.

Biopsy sampling is an effective technique for collecting cetacean skin and blubber samples for various biological studies. However, determining the impact of this research practice is important, as it may vary between sites, species and equipment used. We examined the short-term behavioural reactions of four small (160-278 cm in length) delphinid species (Stenella longirostris, S. attenuata, Tursiops aduncus and Peponocephala electra) to remote biopsy sampling in the vicinity of the island of Mayotte (12°50’S, 45°10’E, SW Indian Ocean). Two scales of behavioural reactions were considered: i) the behavioural reaction of the individual, and ii) the reaction of the focal group to which the targeted individual belonged. Three main categories of behavioural responses were defined on the basis of character and duration: low, moderate and strong. This study underlines that biopsy sampling induces moderate reactions in individuals. No inter-specific variations of responses, at the scale of the individual or focal group, were observed. In other words, smaller delphinids were not more reactive than larger ones. No effect of group size was observed on the strength of behavioural reactions. However, it was clear that biopsy success during sampling sessions was higher in species with large group size. Finally, in the spinner dolphin (S. longirostris), we investigated whether initial behavioural state affected the level of reaction. Resting and socialising groups showed a stronger response than milling and travelling groups. This study confirms the limited impact of remote biopsy sampling in small delphinids, especially in the spinner dolphin. However, as a precautionary approach, wherever possible, biopsy sampling of milling and travelling dolphins may be preferable.

The behavioural development of 90 chimpanzees (Pan troglodytes) was followed. Of these, 65 had been separated from their mothers to prevent casualties or, at a later age, to increase breeding success. Some showed body rocking and others did not. To obtain insight into the causes of the onset and development of body rocking, chimpanzees raised with peers, with their mother, or in a semi-natural group were compared Rocking was never observed in the semi-natural group. It was occasionally seen when with the mother. Separation from the mother soon after birth induced anxiety and rocking developed after sitting upright had developed. Rocking levels of three per cent of the time were still present at seven to nine years of age.

The most probable causes of the development of rocking are frustrating social circumstances and the inability to cope with these. Merging groups, disturbances and the introduction of a fearful object increased rocking in the individuals that had developed the habit. However, rockers reacted less to those circumstances than non-rockers, showing smaller increase in body contact and less reduction of play. This finding suggests that rocking, instead of being a bizarre reaction to unsurmountable stress, could be a behaviour that helps an individual to cope with difficulties and stress. In terms of animal welfare, rocking in chimpanzees housed in laboratory conditions is an indication of a less optimal reaction pattern to frustrating circumstances. The development of rocking may be prevented if the babies are left with the mother and in their social group. Rocking after (late) separation may be prevented when transfer takes place together with familiar peers.

The way in which animals are fed is an important aspect of their welfare. Not only does food provide the energy and nutrients vital for survival, but feeding is also associated with a number of other factors contributing to the well-being of animals. The feeding method can determine the animals’ abilities to fulfil basic behavioural needs, such as foraging. The aim of this paper is to review and discuss the dilemma of choosing between ad libitum feeding (AL) and dietary restriction (DR). AL can produce obese individuals with severe health problems, though it does appear to be compatible with welfare-friendly management systems. On the other hand, DR is often associated with improved physical health and longevity but can leave animals suffering from hunger, frustration or aggression. The species discussed are the laboratory rat, pigs and poultry all of which are omnivores sharing many characteristics in their eating habits. The welfare implications of different feeding methods depend upon the definition of welfare used. Based on a definition of welfare in terms of functioning, DR could be considered the best way to feed animals, because it results in improved physical health and longevity. If welfare is defined in terms of natural living, it is also a requirement for the animal to be able to engage in natural foraging behaviours. From the feelings-based approach, DR can be viewed as preferable only in circumstances when animals are anticipated to live so long that they would otherwise suffer from the negative long-term consequences of AL. It is argued that incentives are needed to make farmers spend resources to ensure that farm animals are allowed to have their foraging-related needs fulfilled. Feeding of laboratory animals creates special dilemmas when it is important either to under- or over-nourish the animals for experimental purposes, in such instances there is a need for Refinement.

White-lipped (Tayassu pecari) and collared (Pecari tajacu) peccaries can be farmed as an alternative to subsistence hunting in Neotropical countries. The animals often show high reactivity to handling, usually involving capture with a net, which is a cause for concern because it poses risks to both animals’ and keepers’ welfare. We aimed to assess the temperament of both peccary species, evaluating animals’ reactions during handling, providing an emotional indicator and a new animal selection criterion for peccary farms. Three indicators were used to assess the temperament of 17 white-lipped and 19 collared peccaries: qualitative behaviour assessment (QBA, by using 12 behavioural adjectives); time to drive each animal through a corridor into a chute (TD, s); and flight speed (FS, m s−1). A Principal Components Analysis was performed for the QBA data to define a temperament index (TI). White-lipped peccaries showed TI scores associated with worse temperament traits than collared peccaries. White-lipped peccaries showed higher TD and FS means than collared peccaries. We found a correlation between TD and FS, but not between TD and TI, nor between FS and TI. The lack of correlation between all temperament indicators occurred, probably, because they measure different aspects of peccaries’ reactions toward humans and the farm environment during handling. A wide phenotypic variability was found among individuals within both species’ populations. The results provide an opportunity to address the role of temperament assessment, improving handling procedures and exploring the possibility of including temperament as a selection criterion in captive breeding programmes.

Leg problems are highly prevalent in modern broiler production and provision of environmental enrichment could be a strategy to improve leg health. Different types of environmental enrichment have undergone evaluation. Our objective was to conduct a systematic review of the effect of environmental enrichment on leg health in broiler chickens. The evaluation of leg health included measures of the entire leg and foot, and behavioural, pathological and physical measures. Six types of environmental enrichment were selected for inclusion: light programme, intensity of light, stocking density, perches, straw bales and separation of resources. For each type, a systematic literature search was performed. The review included 62 studies; 56 randomised trials and six cross-sectional studies. An assessment of the methodological quality of all 56 randomised trials was performed with some reporting deficits regarding occurrence of blinding, randomisation and reliability of measures. Provision of perches and increased intensity of light only displayed limited effectiveness in improving leg health and both mainly affected contact dermatitis. In contrast, there was evidence that a lowered stocking density and a dark/light schedule could improve leg health. Few studies have been carried out on the effect of straw bales and separation of resources. The few studies done have, however, shown that both types of enrichment can be effective in improving leg health. In conclusion, identifying and providing the optimal types of enrichment for broilers will reduce leg problems and increase mobility, thereby improving the welfare of the birds.

Good colostrum management can confer protective immunity to newborn calves, making calves less susceptible to infectious disease, and fundamentally improving both their short- and long-term health, welfare and productivity. Industry recommendations commonly refer to ‘The Three ‘Q's’ of colostrum management: the need for calves to receive sufficient ‘Quantity’ of high ‘Quality’ colostrum ‘Quickly’ after birth; some also include ‘sQueaky clean’ and ‘Quantification of passive transfer’. However, research to date suggests that the failure of passive transfer of colostral antibodies is common on commercial dairy farms, contributing to sub-optimal calf health and mortality. This paper explores why this may be the case by investigating stakeholder perceptions of colostrum management and how these perceptions might affect the practice of ensuring adequate colostrum administration to newborn calves. Calf rearing and youngstock management practices on English dairy farms were investigated using 40 in-depth semi-structured interviews: 26 with dairy farmers and 14 with advisors (including veterinarians, feed and pharmaceutical company representatives). Interviews were audio-recorded, transcribed and thematically coded for analysis. ‘The Three ‘Q's’ were found to act as useful reminders about the goals of colostrum management, and a case can be made for further publicising the inclusion of ‘sQueaky clean’ and ‘Quantification of passive transfer’ as there remains a lack of focus on colostrum hygiene and measurement of successful antibody transfer. Knowledge of the ‘Q's’ did not guarantee implementation, and time and labour constraints alongside farmer misconceptions must be addressed when offering professional advice on improving calf health. Further research to encourage on-farm collection and analysis of monitoring data including rates of passive transfer is particularly needed. Advisors must not overlook the importance of colostrum management when assessing farm practices and ensure that they promote evidence-based recommendations if dairy calf morbidity and mortality is to be reduced.

The resting behaviour of 64 groups of sheep was observed in the lairages of two slaughterhouses after transport for about four hours in a commercial livestock transporter. The sheep were scanned at five minute intervals for the initial three hours in the lairage pen. The median percentage of time spent lying per group was 17 per cent (range 1 to 63). The percentage of time spent lying was not significantly related to the duration of transport, time of arrival or slaughterhouse. There was a significant positive relationship between space allowance (range 0.22 to 0.98 m2 per sheep) and percentage of time spent lying per group (P < 0.001). Increased space allowance also tended to be associated with a decrease in the occurrence of all interactions between the sheep (P = 0.05). Groups penned with two unfamiliar groups tended to spend less time lying than those penned on their own or with one other group (P = 0.07). There was no significant difference in the total percentage of time spent lying by groups penned on slats and those penned on straw. The results indicated that the ability of sheep to rest in the lairage may be compromised by providing low space allowances.

The welfare of farm animals is a policy area that has increased greatly in importance in recent years. When deciding whether a proposed policy should be implemented, it can be useful for policymakers to compare the costs of the proposed improvement with the perceived benefits. The costs are relatively straightforward to calculate but little is known about the benefits. The Contingent Valuation Method (CVM), a direct survey-based method, can be used to shed some light on this. This approach elicits the willingness-to-pay (WTP) for the provision of some public good or service. This paper reports the results of a contingent valuation study of the value of welfare improvements for growing pigs. Attitudes and opinions with regard to farm animal welfare are explored and WTP elicited for various pig welfare improvements including increases in space allowance, environmental enrichment and research into improved pig housing design. The results reveal a positive WTP for these improvements. However, it is also noteworthy that a significant proportion of the general public is willing to pay nothing for these improvements. Overall, the study illustrates the usefulness of the CVM approach as a tool for policymakers in assessing the merits of possible policy initiatives affecting the welfare of animals.

Several studies have shown that finishing bulls kept in housing systems with fully slatted concrete floors are impaired in their lying behaviour and locomotion due to the hardness and slipperiness of the ground. The aim of this study was to investigate the effects of floor quality (straw-bedded lying area, rubber-coated slats, concrete slats) on the lying behaviour of finishing bulls. The floor type did not influence the total lying duration, but the number of lying bouts and the number of short standing periods decreased with increasing hardness of the floor in the lying area. In addition, bulls showed more interrupted lying-down and standing-up movements on concrete and rubber-coated slats than on straw bedding. On the other hand, atypical lying-down and standing-up instances, slipping and falling were reduced on rubber-coated slats and on straw bedding compared to concrete slats. Displacements of lying animals were observed more often on rubber-coated slats than on concrete slats or straw bedding. In conclusion, rubber-coated slats improve traction on the ground but are not able to provide as soft a lying area as straw bedding. Rubber coating can thus serve to improve welfare for finishing bulls kept on concrete slats but cannot be considered equivalent to straw bedding.

A haulage company, transporting pigs for a marketing group, has monitored information about journeys and kept records of deaths in transit (DIT). These data can be used to set guidelines and they highlight those areas of pig transport that need further study.

Factors, other than genetic ones, which may contribute to the rate of DIT were considered. A strict seasonal effect was not apparent, but where conditions were hot and wet there was an increase in DIT. There was also a ‘time of day” effect, with transport in the afternoon showing a higher rate of DIT than transport in the morning. It was observed that if pigs were deemed dirty at loading then DIT were more likely. Clean pigs at loading could indicate that hygienic and environmental conditions in the finishing stage were good. Individual producers showed a wide variation in their rate of DIT, with some producers regularly experiencing a high level Routine monitoring of the rate of DIT and transport conditions could help to pinpoint the source, and possible cause, of the problem.

Information that helps in the understanding of transit deaths is important because if stress in the period from selection to slaughter can be reduced, there should be both economic as well as welfare benefits.

Dehydration is a serious welfare issue for equines working in developing countries. Risk factors such as high ambient temperature, heavy workload and poor water availability are exacerbated by the traditional belief that provision of water to working animals will reduce their strength or cause colic and muscle cramps.

As part of the welfare assessment of 4889 working horses and donkeys during 2002/3, eight observers were trained to perform a standardised skin tent test. The prevalence of a prolonged duration of skin tenting was 50% in horses and 37% in donkeys. Two studies investigated inter-observer repeatability of skin tent test techniques, using a total of 220 horses and donkeys in India and then Egypt: measures of agreement with a ‘gold standard’ observer varied from 40 to 99%. Simplifying the test by reducing the number of possible scores for skin tent from three (immediate return of skin to normal position; delayed return up to three seconds; delayed return more than three seconds) to two (immediate return of skin to normal position; delayed return of any duration) did not improve overall repeatability of the skin tent test. Potential reasons for not achieving high levels of agreement include variations in assessment method, assessors' previous experience, subjective demarcation between score categories and biological variability.

Following the 2012 European ban (1999/74/EC) of conventional battery cages, only furnished cages will be allowed for laying hens. However, even when furnished cages provide a pecking and scratching area most dustbathing occurs on the wire floor. This study aimed to investigate whether laying hens showed a preference for dustbathing on a covered wire floor rather than a conventional wire floor. Eight groups of 10 hens were housed in pairs of adjoining furnished cages. All hens were leg-ringed for individual identification. Each pair of cages consisted of one cage containing a wire floor covered with perforated Astroturf and a second cage containing a conventional wire floor, joined by a pophole through which hens had unrestricted access. Initial scan samples were taken to investigate the general behaviour of the hens on each floor type. Dustbathing was observed more frequently on Astroturf and no other behaviours were affected by the floor type. Scans were subsequently taken at 5 min intervals between 1130h and 1600h for 10 days, identifying the number of hens, and which individual hens, were dustbathing on each floor type. Data were analysed on a per cage basis, using the binomial sign test. A strong preference was found for dustbathing on Astroturf flooring that was apparent in all cages. Furthermore, the distribution of hens indicated this was not attributable to any overall preference for either floor type but was specific to periods when hens were dustbathing. This demonstrates that there is the potential to include Astroturf in the design of furnished cages, as a dustbathing substrate, in order to improve the welfare of laying hens.

The aim of this study was to assess brain activity in lambs during slaughter without stunning and its correlation with heart rate (HR) and the absence of physiological reflexes. The index of consciousness (IoC-view®) assesses consciousness by an algorithm that analyses the EEG and gives an index from 0 (unconscious) to 100 (conscious). Eight lambs (Merino breed) of 20 to 25 kg live weight were individually restrained in a stretcher and three skin electrodes were placed at the level of the frontal bone. The electrodes were connected to the IoC-view®. Two additional electrodes were placed on the chest and the HR was transferred to a watch monitor. Recording time started 2 min prior to sticking to attain the basal IoC and HR value of each animal. During bleeding, presence of corneal reflex and rhythmic breathing were recorded every 10 s until brain death. Rhythmic breathing disappeared at an average time of 44 (± 4.2) s after sticking, ranging between 30 and 60 s. The corneal reflex disappeared at 116 (± 11.01) s, ranging between 80 and 160 s after sticking. Changes in the brain activity occurred between 22 to 82 s after sticking (52 [± 20.2] s). In five out of six animals the HR increased (177 [± 22.7] beats min−1) compared to basal values (139 [± 8.4] beats min−1) at 115 (± 97.5) s after sticking. Both brain activity and physiological reflexes revealed that when bleeding is performed, through a transverse incision across the neck without stunning, the onset of unconsciousness could last 1 min which impaired animal welfare. This prolonged consciousness compared to other authors’ findings may be attributable to inefficient bleeding when lambs are slaughtered without head restraint.

Captive polar bears (Ursus maritimus) are well-documented as being prone to behavioural disorders and, as a result, their welfare is the cause of increasing concern. There is therefore a need for an evidence-based approach to the assessment of the welfare of this species and identification of valid welfare indicators is the first step towards achieving this. To this end, a critical evaluation of peer-reviewed literature was undertaken. Searches of Web of Science and Scopus took place in May 2020 for publications relevant to the welfare of captive polar bears which met inclusion criteria. Further, validity of extracted indicators was assessed via investigation of evidence of content, construct and criterion validity along with strength of evidence at publication-level. Database searches and snowballing unearthed 46 publications included for review. Identified indicators were sorted into nine behavioural, four physiological (based on physiological or biological sampling) and five physical (based on visual inspection) categories. Among behavioural indicators, the strongest evidence of validity was found for abnormal behaviour. For the physiological indicators, validity was only established for faecal glucocorticoid metabolite concentration. Content validity was assumed for all physical indicators. Generalisability and strength of evidence was generally compromised by low sample sizes and experimental limitations, and only a small number of papers investigated welfare indicators directly, resulting in a paucity of validated indicators. Potential welfare indicators that warrant further validation are highlighted. Overall, this review provides an overview of current valid and promising welfare indicators along with identified gaps in knowledge, relevant for the provision of a methodology for assessing and monitoring welfare of captive polar bears.

It is generally accepted that to carry out certain trials or procedures, particularly metabolism or digestibility studies, it is necessary to house animals singly, often in sterile metal cages which differ greatly from the animal's normal living accommodation. The lack of choice, mental and physical stimulation and general ‘creature comforts’ increases the stress of isolation.

The design of the buildings at the Waltham Centre for Pet Nutrition aim to ameliorate these unsatisfactory conditions and provide environmental enrichment, freedom of choice and mental and physical stimulation, in housing as similar as possible to the normal housing of domestic pets.

Attitudes to animal biotechnology are diverse, partly because people have different viewpoints and often do not recognize or acknowledge this to be so. First, people adopt different ethical approaches. If an opponent of genetic manipulation says’ I don't like the idea of altering animals ‘biology’ and a proponent replies’… but it is useful’, they are failing to communicate, because one is asking whether the action is right or wrong, whereas the other emphasizes the consequences. Another approach focuses on the person carrying out the action. Many people have hybrid views combining elements of these different approaches. Second, people ‘s concepts of welfare vary, emphasizing animal minds, bodies or natures - or a combination of these. A proponent who argues that a particular genetic change will not cause suffering is unlikely to reassure an opponent who puts more emphasis on naturalness than on feelings or health. An improved dialogue, in which people attempt to understand one another's viewpoints, may enable common principles to be established and practical measures to be taken that enable more cooperation in attempts to improve both human and animal welfare.

Methods to assess changes in the mental state of animals in response to their environment can be used to provide information to enhance animal welfare. One of the most profound changes of mental state observable in mammals is the change between wakefulness and sleep. Sleeping mammals have characteristics that are similar to one another and are measurable, such as specific behaviours, changes in responsiveness to external stimuli and changes in electrophysiology and neurochemistry. Although sleep is a ubiquitous behaviour in the life of mammals, there has been relatively little research on this topic in domesticated animals. All animals are motivated to sleep and this motivation increases after a prolonged period of wakefulness. In humans, sleep can be affected by what has occurred in the prior period of wakefulness and this has also been demonstrated in some non-human mammals. An important aspect of human sleep medicine is the association between stress and subsequent sleep disturbances. Studying changes in amount, bout length, distribution or type of sleep after exposure to potentially stressful events, could help us understand how animals respond to changes in their environment. It is possible that different types of stressors could affect sleep characteristics in different ways and that monitoring and identifying these changes could be useful in providing an additional way of identifying management procedures that have the potential to affect welfare. Sleep measurement is a potentially valuable tool in studies to assess animal welfare.

Finding a responsible method of population control that does not compromise animal welfare is a pressing problem for zoological institutions and conservation breeding programmes. This is exemplified by the conservation breeding programme of the golden-headed lion tamarin, Leontopithecus chrysomelas. The number of golden-headed lion tamarins in captivity is currently being limited by, among other means, the use of contraception. We have conducted a study on the effects of contraceptive methods used in golden-headed lion tamarins. Data were collected through the distribution of a survey. The use of Melengestrol acetate (MGA) implants in females was by far the most widespread contraceptive method. It was very effective in preventing reproduction, provided that females were not pregnant at the time of implantation. Pregnancies that had commenced before MGA implantation were carried to term and resulted in viable infants, as far as noted. However, the degree of reversibility was very low and, if females did conceive after MGA implantation, infant survival was lower than expected. The widespread use of MGA implants in golden-headed lion tamarins (and probably other species) should be seriously reconsidered. Alternative methods of population control should be investigated. Possible options include the use of other contraceptive methods, limiting the number of offspring through natural factors and the use of euthanasia under very strict conditions. Animal welfare implications associated with the use of euthanasia are discussed.