The effect of fish-meal supplementation or cimaterol administration on the composition, cellularity and metabolism of subcutaneous and perirenal adipose tissue was examined in young Friesian steers given grass silage. Animals (approx. 117 kg live weight) received either silage alone (group C; no. = 6) or supplemented with fish meal (150 g/kg silage dry matter; group FM; no. = 6) or cimaterol (0·06 mg/kg body weight per day) administered via osmotic minipumps (group CIM; no. = 6) for a period of 6 weeks. Samples of perirenal and subcutaneous adipose tissue obtained at slaughter were used for determination of tissue composition and in vitro rates of lipogenesis, lipolysis and substrate oxidation. FM animals showed significantly greater live-weight gains (0·61 kg/day) compared with C (0·21 kg/day, P < 0·001) while CIM animals had significantly lower rates of gain (0·04 kg/day, P < 0·05). This was considered to be related to increased heat loss from the β-agonist-treated animals as a result of very cold weather encountered during the experimental period. CIM administration reduced adipocyte size suggesting an increased number of cells per g tissue. Both lipolytic and lipogenic rates were reduced by cimaterol and there was some evidence that the response to insulin was diminished, at least with respect to substrate oxidation. FM increased lipogenesis from acetate and acetate oxidation rates in subcutaneous adipose tissue with similar trends in perirenal tissue. The results indicate that both fish-meal supplementation and cimaterol administration caused substantial changes in adipose tissue metabolism as determined using in vitro procedures, even though the magnitude and direction of the changes were not in strict accord with estimates of net fat accretion from related in vivo studies.

The effects of breed and feeding on the prediction of carcass fat and carcass muscle using rump ‘P8’ subcutaneous fat thickness were investigated in heavy-weight beef carcasses (277 to 512 kg). Hot side weight, eye muscle area and their combination added to fat thickness were evaluated as additional predictors. The predictions of carcass composition from grass-fed steers were validated on data from grain-fed steer carcasses. There were differences between breeds and feeding regimes in the level of tissue proportions at a given fat thickness, but no differences in regression slopes. Fat thickness alone was, therefore, not an accurate indicator of the proportions of side fat or side muscle in heavy-weight carcasses. In the grain-fed steers, the correlations between P8 and tissue proportions were relatively strong, and the addition of hot side weight and eye muscle area, alone or in combination, did not significantly improve the predictions. Conversely, in grass-fed steers where the relationships between carcass composition and fat thickness were weaker, the addition of hot side weight and eye muscle area made a significant contribution to prediction. In the predictions of tissue weights, hot side weight and eye muscle area when added to fat thickness, significantly contributed to the prediction of side muscle weight for both feeding systems. These three variables did not account for the observed differences between breeds or feeding regimes in the proportion or weight of fat, nor in the proportion of muscle; but they effectively eliminated these groups' differences in the estimation of muscle weight.

Twelve Suffolk-Finn Dorset lambs were reared from 25 to 40 or 25 to 55 kg body weight on either pelleted dried grass or a ration of pelleted grass plus barley (ratio 1:1) in a comparative slaughter experiment designed to determine the amounts of total nitrogen and individual amino acids accreted in different body components during growth. Nitrogen (N) balance measurements were determined frequently during this growth phase and accumulated N retentions were compared with the total N accretion determined by comparative slaughter. Total N and individual amino acids accumulated in carcass, wool, skin, offal and blood, head and feet, gastro-intestinal tract and liver were linearly related to body weight in all cases other than for cysteine in carcass. At 25 kg live weight, proportionately 0·52 of total body N was in carcass components, 0·115 in wool, 0·08 in skin, 0·10 in offal and blood, 0·095 in head and feet, 0·06 in the gastro-intestinal tract and 0·02 in liver. However as the animals grew from 25 to 55 kg, 0·256 of the total N accretion was in wool, which was rich in cysteine (98 g/kg total amino acid). Carcass accretion represented only 0·449 of total body N accretion. The N balance technique overestimated net protein accretion by 0·24 (s.e. 0·036).

Two experiments were designed to examine the effects of ammonia treatment of barley straw on rumen environmental conditions for fibre degradation, and to determine the effect of treatment on the soluble and insoluble fractions of straw. In experiment 1, rumen pH, and ammonia-nitrogen and volatile fatty acid concentrations in the rumen of sheep receiving untreated (US1) or ammonia-treated (TS1) straw were recorded. Also, both straws were incubated in the rumen of both groups of animals. There were no significant differences (P > 0·05) within rumen parameters, except for butyrate proportion, which was higher (P < 0·01) with TS1. Dry matter and neutraldetergent fibre disappearances of TS1 were higher (P < 0·001) than those of US1 at all the incubation times. Degradation of both straws in US1-fed sheep was not smaller, and in some periods of incubation was even greater, than in TS1-fed animals. In experiment 2, samples of untreated (US2) and treated (TS2) straw were incubated in wethers offered a mixture (50:50) of alfalfa hay and straw. Samples of US2 and TS2 were soaked in autoclaved rumen fluid to measure their solubility. It was clear that, although treatment increased the soluble fraction of US2 and TS2 by 52 and 65 g/kg respectively, from 0 to 72 h of incubation, increasing the differences between straws from 45 to 58 g/kg in that period, actual microbial action on the treated roughage was the more important factor, accounting for a difference between US2 and TS2 of 131 g/kg after 72 h incubation.

Genetic parameters for birth and 240-day weaning weights in Gudali and Wakwa cattle breeds in a tropical environment in Cameroon were estimated by derivative-free restricted maximum likelihood procedure using singletrait animal models with maternal effects. Performance data covered the period 1971 through 1985. Heritability estimates for direct effects of live weights at birth and weaning were 0·39 (s.e. 0·10) and 0·27 (s.e. 0·09) in Gudali cattle and 0·65 (s.e. 0·15) and 0·29 (s.e. 0·13) in Wakwa cattle, respectively. Corresponding heritability estimates for maternal effects were 0·06 (s.e. 0·06) and 0·20 (s.e. 0·09) in Gudali cattle and 0·22 (s.e. 0·08) and 0·27 (s.e. 0·12) in Wakwa cattle. Estimates of genetic correlations between direct and maternal effects were negative and substantial for birth weight in Gudali (0·86) and Wakwa (-0·93) and for weaning weight in Gudali (-0·68). The estimate for weaning weight in Wakwa (-0·39) was lower. It was evident from the results that selection on individual performance was likely to yield substantial genetic progress in these traits. Although heritability estimates for maternal effects were low, the negative direct-maternal genetic relationships indicated that selection for these traits should not ignore the maternal component.

Twenty-five male goats weighing 16 (s.e. 1-5) kg and aged 15 months were used to measure the dry matter (DM) intake of five browse species namely Acacia albida, Tamarindus indica, Etanda africana, Anogeissus leiocarpus and Sterculia setigera in a growth trial lasting 16 weeks. This was followed by a digestion trial with five goats in a 5 × 5 Latin square with 10 days adaptation and a 5-day measurement period. The degradation characteristics of the browse were measured by incubating samples in nylon bags for 6, 24, 48 and 96 h in the rumens of three sheep fitted with rumen cannulae and given hay plus grass nuts. The exponential model P = a +b(l — ect) was fitted to the data. Rumen fluid from the sheep was also used as an inoculum to incubate the samples in vitro for 3, 6,12, 24, 48, 72 and 96 h. Nylon bag degradability results were compared with in vivo results and in vitro gas production. The mean DM intakes, apparent digestible DM intakes and growth rates were 0·60, 0·62, 0·55, 0·53 and 0·65 kg/day, 0·43, 0·43, 0·35, 0·34 and 0·49 kg/day and 55, 60, 49, 42 and 62 glday for A. albida, T. indica, E. africana, A. leiocarpus and S. setigera respectively. Using the degradation characteristics A, B and c in a multiple regression analysis, the correlation coefficients with DM intake, apparent DM digestibility, apparent digestible DM intake and growth rate were 0·99, 0·88, 0·92 and 0·99 respectively. The inclusion of a lag phase (L) instead of A in the regression analysis improved the prediction of apparent DM digestibility and apparent digestible DM intake. The correlation coefficients between DM loss in nylon bags and in vitro gas production at 6, 24 and 48 h incubation were 0·84, 0·83 and 0·90 respectively. The results indicate that it may be possible to predict DM and apparent digestible DM intakes of browse by goats from the rumen degradation characteristics.

Six ewes were given a white clover/grass silage with a high content of cyanogenetic glycosides during the last 69 to 97 days of pregnancy (group CN+), while the five ewes of the control group (group CN−) received a white clover/grass silage with a low content of cyanogenetic glycosides during the last 67 to 92 days of pregnancy. Uniodized salt was the only mineral supplement offered. The daily intake of cyanide (in the form of cyanogenetic glycosides) and of selenium per animal was 1000 and 60 mg of cyanide and 30 and 23 μg of selenium in group CN+ and CN− respectively. Initial erythrocyte glutathione peroxidase activity (GPx) was identical in both groups. Two weeks before parturition GPx activity was significantly lower in the ewes of group CN+ than in the ewes of group CN−. The newborn lambs of the group CN+ had significantly lower erythrocyte GPx activities (P < 0·01) and tended to have higher plasma creatine kinase activities (P = 0·07) than the lambs of the group CN−. The results show that the intake of cyanogenetic glycosides affects the selenium status of sheep and increases the susceptibility of lambs to nutritional myopathy if the selenium content of the ration is marginal.

Plasma thyroxine concentrations in the ewes in group CN+ were not reduced but their lambs tended to have slightly lower levels immediately after birth than the lambs of group CN− (P = 0·25). The intake of cyanogenetic glycosides presumably had no or at most only a slight effect on thyroid function, presumably because the body iodine reserves of the ewes were sufficient to offset the goitrogenic effect of thiocyanate.

The effect of grazing clover on ewes and lambs was studied using nitrogen-fertilized grass (G) and grass/white clover pastures (GC) containing proportionately 0·18 clover. Carcasses of lambs grazed on GC were significantly heavier than carcasses of lambs grazed on G pastures. An interaction with weaning occurred. Carcasses from lambs grazed on GC for 53 days post weaning were 2·3 kg heavier (F < 0·01) but lambs slaughtered at weaning had similar carcass weights. Diet had no effect on relative joint components of carcasses. The effect of clover in the diet on carcass weight was attributed to higher levels of protein retention.

Analysis of the fatty acid composition of intramuscular and subcutaneous and perirenal lipids indicated small but significant effects of diet. Lean tissue lipids of lambs grazing clover showed significant increases in C18:2 and reduced C20: 5 fatty acids. In tissue fats there were small increases in C14:0, C16:0 and C18:2 with reduced C18:1 content.

Twin suckled lambs grazing GC for 94 days from turn-out in early April showed increased live-weight gain over lambs on G of 336 v. 287 g/day (s.e.d. 84; P < 0.001). Post weaning live-weight gain of lambs was 173 and 221 g/day on G and GC respectively. Sward height was maintained at 5.18 and 5.24 (s.e.d. 0.075) cm on G and GC paddocks by adjusting ewe numbers. GC pastures carried proportionately 0.82 of the stock on G pastures but output of lamb was similar at 1289 and 1247 kg/ha for G and GC respectively.

Results showed that the production penalty of lower stocking rate associated with grass/clover v. grass fertilized with 190 kg nitrogen per ha was ameliorated by higher lamb live-weight gain and carcass weight without change in joint composition or nutritionally significant change in fatty acid composition of carcass tissues.

An experiment was designed to examine the interaction between birth status and plane of nutrition of calves in relation to postnatal and lifetime performance. The experiment was a 2 × 2 × 2 factorial arrangement of birth status (single and twin), plane of nutrition from birth to 16 weeks (period 1) and plane of nutrition from 16 weeks to slaughter at a mean live weight of 530·4 (s.e. 0·13) kg (period 2). The planes of nutrition were control and low with metabolizable energy (ME) intakes of 32·1 and 21·4 (s.e. 0·46) MJ/day from birth to 16 weeks, and 73·9 and 64·1 (s.e. 0·95) from 16 weeks until slaughter. The birth weights of the single and twin-born calves were 47·2 and 39·7 (s.e. 0·90) kg respectively. There was no interaction between birth status and plane of nutrition in either main period and by 40 weeks of age the live weights of the twin calves were similar to those of the single-born calves. For period 1 plus period 2 the live-weight gains per unit of ME intake were 12·4 and 13·1 (s.e. 0·28) g/Mf for single and twin-born calves respectively. Although twins had slightly poorer carcass conformation and higher proportion of bone in the carcass, the concentrations of saleable meat and high-priced joints in the carcass were similar. Birth status had no effect on the ultimate pH of muscle. It is concluded that viable twins have similar beef producing potential to single-born calves.

The control and low planes of nutrition imposed during the periods from birth to 16 weeks and 16 weeks to slaughter produced live-weight gains of 822 and 573 (s.e. 31·0) g/day, and 857 and 690 (s.e. 12·6) g/day respectively. Calves which had been on the low plane during the initial period had a compensation index of 0·30 by 64 weeks of age. Carcass measurements were not affected by the plane of nutrition during either period. Steers grew faster than heifers, had lower area o/m. longissimus dorsi but less separable fat and more bone in the forerib joint than heifers at equal carcass weight.

Six rumen cannulated sheep (26·1 (s.d. 2·24) kg live weight) given maize stover ad libitum were supplemented with either sunflower (Helianthus annus) cake (SFC), decorticated cottonseed (CSC) or noug (Guizotia abyssinica) cakes (NGC) with or without crushed maize grain. The 6×6 Latin-square experiment studied the degradation properties of the oilseed cakes and their effects and that of maize on the degradation of maize, maize stover and the composite diet.

Seedcakes differed in the degradability characteristics of dry matter and nitrogen: intercept (a) (P < 0·001); potentially degradable fraction (b) (P < 0·001); rate of degradation (c) (P < 0·001); and the effective degradability (P < 0·0001). The pooled sum of the degradabilities of the individual ingredients were significantly lower than that of mixed ingredients (composite diet) (P < 0·001).

The main effects of protein and maize were not significant on the degradation of maize stover. Maize had the tendency to depress the rates of degradation of stover by 0·52 with SFC.

Although the protein × maize interaction was not significant on maize stover degradability, it is important to note that maize had the effect of increasing stover degradation rate with CSC and NGC, but a depressing effect with SFC. Hence it was concluded that the carbohydrate effect on roughage degradation rate may vary as a function of the difference between the degradation rate of the energy and protein supplements.

Thirty-six male Ethiopian Menz sheep (9 to 22 months old, average live weight 15·8 (s.d. 1·84) kg), given maize stover (1·5 times ad libitum) supplemented with either 75 g cottonseed cake (CSC), 114 g noug cake (NGC; Guizotia abyssinica) or 112 g sunflower cake (SFC) with or without maize grain, were used in an 88-day study comprising growth and balance trials. The trials were undertaken according to a randomized-block design with a 2 × 3 factorial arrangement.

There were no significant interactions (P > 0·05). Sheep consumed significantly more stover when supplemented with CSC compared with NGC and SFC (P < 0·05). Maize grain significantly increased organic matter intake (P < 0·001). Although CSC tended to support lower live-weight gains, the effect of protein was not significant. Maize grain increased live-weight gains (P < 0·01).

Urinary nitrogen (N) excretions were similar between CSC and SFC but about 0·22 higher with NGC (P > 0·05). The faecal N output was 0·33 (P < 0·01) and 0·18 (P < 0·05) higher with CSC than with NGC and SFC respectively. Maize grain had no effect on any of the N-balance measurements.

Cottonseed cake supported lower daily production of purine derivatives (PD) (P < 0·01), microbial purine (P < 0·01) and microbial protein (P < 0·01) than either NGC or SFC. Maize grain increased the daily excretion of total PD (P < 0·05), microbial purine (P < 0·05) and microbial protein (P < 0·05). Neither the protein source nor maize grain affected the efficiency of microbial protein synthesis. It was concluded that SFC was utilized more effectively both in terms of rumen microbial N synthesis, N retention and growth. The addition of a small amount of energy as crushed maize grain increased microbial N synthesis, N retention and live-weight gain.

The performance of British Saanen, Boer × British Saanen and Anglo-Nubian kids from weaning to slaughter at 28, 33 or 38 kg live weight (LW) was examined in a 3 × 3 factorial experiment. Kids were reared on a concentrate and hay ration, after weaning at 8 weeks of age. Intakes were measured individually. At slaughter, carcasses and body fractions were weighed, left half-carcasses were dissected into muscle, fat and bone, and the carcass and non-carcass fractions were analysed chemically. There ivas no significant effect of breed type on mean daily intakes, but there was a significant breed-type effect on mean daily LW gains in the order British Saanen > Boer × British Saanen > Anglo-Nubian kids. Compared with the pure British Saanen kids, the Anglo-Nubian kids produced heavier carcasses yielding more muscle and less subcutaneous and intermuscular fat at each slaughter weight. By contrast, the Boer × British Saanen carcasses contained proportionately more fat, mainly intermuscular fat, than the British Saanen when slaughtered at 28 or 33 kg LW. When taken on to slaughter at 38 kg LW, the Boer × British Saanen kids showed little further increase in fat deposition at the internal and intermuscular sites compared with the British Saanens, resulting in carcasses with slightly more subcutaneous fat but lower overall carcass fat content.

A survey of dairy herds was undertaken to establish an empirical model for the goat lactation curve. The adopted model will be used to predict milk output from commercially kept dairy goats. It is also desirable for the model parameters to be easily interpretable in terms of shape and scale of the curve.

Records for 152 goat lactations from a Research Institute and 255 lactations in five commercial herds were analysed. Three groups of equations were fitted to individual goats from the Research Institute herd. The most suitable model was selected after study of residuals and consideration of the model parameters.

The adopted model was of the form: y = A exp(B(1 + n '/2)n' + Cn ‘2 – 1·01‘sol;'n) where y = daily yield in kg; n = day of lactation (post parturition); and n' = (n - 150)/100.

This model was then validated with lactation data from the five commercial herds. There was no evidence to reject the adopted model.

The effects of melatonin implants, administered during anoestrus, on the time of onset of oestrus, conception rate and litter size in indigenous (Capra prisca) and crossbred dairy goats were studied on commercial farms in a hill region of northern Greece. In the 1st year, 192 indigenous and 86 crossbred does on one farm (farm 1) were each separated into treated (T) and control (C) groups. The T groups from each breed type were given implants of melatonin on 17 April and 6 May, respectively. Bucks were re-introduced to both T and C groups 5 weeks after implantation. Mean mating dates for indigenous goats were 6 July (s.e. 0·15) (T) v. 13 July (s.e. 0·24) (C). Corresponding dates for crossbreds were 31 July (s.e. 0·61) (T) v. 14 August (s.e. 0·87) (C). Treatment did not significantly affect conception rate. Mean kidding dates (from first service) were 6 December (s.e. 0·18) (T) v. 14 December (s.e. 0·28) (C) for indigenous and 31 December (s.e. 0·64) (T) v. 13 January (s.e. 0·82) (C) for crossbred goats. Litter size was higher in both T groups but the difference was not significant. In the 2nd year the indigenous flock on farm 1 was supplemented by two similar neighbouring farms. Farm 1 had both treated does and control does; farm 2 were all treated and farm 3 were untreated control does. In this year all bucks used on treated does were also implanted. All implants were given on 21 April. Mean mating dates were 21 June (s.e. 0·23) (1/T) v. 9 July (s.e. 0·36) (1/C), and 28 June (s.e. 0·09) (2/T) v. 19 July (s.e. 0·15) (3/C). Conception rate was high in all groups. Corresponding mean kidding dates were 22 November (1/T) v. 11 December (1/C) and 29 November (2/T) v. 22 December (3/C). Litter size was higher (F > 0·05) in T than in C groups.

Seventy-six Large White × Landrace sows from parities 2 to 6 were allocated to one of four treatments at day 110 of gestation on a parity and live-weight basis. The four treatments involved food levels of either 3 or 6 kg/day during a 28-day lactation followed by either 1·75 or 3·50 kg/day during the first 28 days of gestation. Litter size suckled was standardized at 10 piglets wherever possible. Live weight and backfat changes were monitored in all sows and litter growth and survival rates were recorded. Post weaning all sows were given daily boar contact and mated twice at the first observed oestrus. A single blood sample was collected from each sow at days 7, 14, 21 and 28 of gestation for determination of plasma progesterone concentration. Data on litter size and piglet birth weights were collected at the subsequent parturition. Sows low-fed during lactation lost significantly more live weight (31·2 v. 5·8 kg, P < 0·01) and P2 backfat (3·6 v. 1·9 mm, P < 0·05) in lactation than did high-fed sows. Underfeeding the lactating sow also significantly reduced the weaning weight of the litter (62·3 v. 68·7 kg for low- v. high-fed sows, P < 0·05), but did not significantly change either creep food intake by the litter or pre-weaning mortality rate. Parity 2 sows weaned significantly heavier piglets and litters than did sows from parities 3 to 6 (7·48 and 74·9 kg v. 6·72 and 63·8 kg respectively, P < 0·01). Food level in lactation did not significantly influence the length of the rebreeding interval (6·3 v. 7·3 days for high- v. low-fed sows) and although there was a two-fold difference in the proportion of sows failing to exhibit oestrus post weaning (0·08 v. 0·16 for high- v. low-fed sows) this difference was also not significant. Lactation food level did not significantly influence either sow live-weight or backfat change in gestation, plasma progesterone concentrations during early gestation or subsequent litter size. However, sows low-fed in lactation did tend to produce fewer piglets in the subsequent litter (9·54 v. 10·75 piglets born alive for low- and high-fed sows respectively, P = 0·062). Post-weaning reproductive performance was significantly affected by sow live weight (P < 0·05) and backfat (P < 0·01) at parturition, and live weight (P < 0·01) and backfat (P < 0·05) at weaning, but not by changes in these parameters during lactation. Food level in early gestation did not significantly influence either plasma progesterone concentrations, the size of the subsequent litter (10·09 v. 20·29 piglets born alive for high- and low-fed sows respectively) or mean piglet birth weight (1·27 v. 2·22 kg for high- and low-fed sows respectively). It is concluded that (1) both lactation food level and parity may alter pre-weaning piglet growth, and (2) the effects of lactation food level on the subsequent reproductive performance of mature sows appear to be different from those seen in parity 1 sows.

The following goat lactation model was fitted (using non-linear regression) to 407 lactations from five commercial goat dairies and one Research Institute goat herd: y = A exp (B(l + n'/2)n' + Cn' 2 - 1·01/n) where y = daily yield in kg; n = day of lactation (post parturition); and n' = (n -150)1100.

Influence of farm, parity and season on the parameter estimates for 376 individual lactations was studied, using multiple linear regression. The models adopted were of the form: A = 1·366 + 1·122 × parity - 0·137 × parity2; ln(-B) = - 1·711 + 0·107 × parity + 0·512 season one; C = 0·037, with a standard deviation for A of 0·658, for ln(-B) of 0·636 and for C of 0·127.

Influence of litter size on parameters was investigated for the Research Institute herd. There was no evidence of an effect on any of the model parameters.

Ten hays harvested at three stages (early bloom MB, mid bloom MB or in seed) made from lucerne (Medicago sativaj, sweet clover (Melilotus segetalis), Persian clover (Trifolium resupinatum) and pre-bloom (PB) Italian ryegrass (Lolium multiflorum var.), were offered ad libitum to four Merino male sheep and daily intake (g dry matter (DM) per kg M0·75) and DM apparent digestibility (DMD) were measured. In sacco DM degradation (g per 100 g DM), gas production (ml per 200 mg DM), in vitro digestibility and fibre composition (g/kg DM) of the hays were also studied. Gas production or DM degradation were calculated at 6, 12, 24, 48, 72 or 96 h and their kinetics were described using the equation p = a + b(1 - e-ct). Intake and in vivo DMD of the hays were variable (P < 0·01). Lucerne EB and Persian clover (all stages) had the highest nutritive value, whereas sweet clover (all stages) had the lowest. Apart from neutral-detergent fibre, which was only related to intake (r = -0·68; P < 0·05), chemical components and in vitro digestibility were poorly (P > 0·05) related to animal performance. Between 12 and 96 h incubation, intake and in vivo DMD were better related to DM degradation (r = 0·79 to 0·83; r = 0·61 to 0·77) than to gas production (r = 0·73 to 0·80; r = 0·58 to 0·78). Prediction of intake and in vivo DMD from the (a + b) values did not provide a great advantage over using some of the static values of gas production or DM degradation.

Multiple regression using separated kinetics of degradation resulted in highest accuracy for predicting intake and apparent digestibility from gas production (R2 = 0·63; R2 = 0·78) and nylon bag degradability (R2 = 0·77; R2 = 0·89). It was concluded that the gas test has good potentiality as it was capable of predicting not only apparent digestibility, but also intake to a level close to that of the nylon bag technique.

A theoretical function is proposed which describes the individual weight-age relationship for foetuses in pigs. The function is based on total growth of the litter. A maximum uterus capacity, dependent on insemination weight of the sow, and a limitation of this capacity, dependent on litter size, is assumed. A modified Michaelis-Menten function is used as a basic growth function. The model was tested against experimental data and compared with four models from the literature. Goodness of fit, extrapolation outside the range of data, and biological interpretation of the parameters from the model were checked. A maximum uterus capacity of 20·3 kg for the average insemination weight of 119·0 kg was found. The inflexion point of the model was significantly (P < 0·05) dependent on litter size.

An investigation was conducted to compare, the LH, prolactin and progesterone profiles of ewe lambs with ewes during oestrus and early pregnancy. Peak LH concentration was significantly lower (P < 0·05) and duration of release tended to be shorter during oestrus in ewe lambs. Furthermore, this smaller LH peak tended to be associated with a lower ovulation rate. LH pulse rate, amplitude and concentration were similar in both groups during day 13 of pregnancy. However, LH concentration was higher in ewe lambs compared with ewes on day 28 of pregnancy. Peak prolactin concentration was lower during oestrus in ewe lambs (P < 0·05) but there was no difference between the two groups during early pregnancy. Plasma progesterone concentrations were similar in both groups until day 13 of pregnancy. Thereafter, mean progesterone concentration was lower, from day 14 to 30, in the ewe lambs (P < 0·05). These results indicate that in ewe lambs and ewes maintained under identical conditions plasma hormone profiles differ during oestrus and early pregnancy. Moreover, these differences probably contribute towards the lower reproductive performance of ewe lambs.

Estimations of purine derivatives excretion and urinary-nitrogen loss were used to test the response of rumen fermentation to supplementation of straw with different sources of carbohydrate. Two groups of Rasa Aragonesa ewes (44 (s.e. 0·75) kg live weight were given ad libitum basal diets of either ammonia-treated (ATS) or urea-supplemented (USS) barley straw, with 12 animals per basal diet group. Three supplements, barley grain, sugar-beet pulp or grass hay, respectively, were given to each basal diet group, giving a total of six dietary treatments with four animals per treatment group. Four levels of supplementation were studied (150, 300, 450 and 600 g air dry matter per day), one in each of four experimental periods. Each 45-day experimental period comprised 38 days of adjustment followed by a 7-day measurement period. Digestible organic matter (DOM) intake was higher in animals receiving ATS than in animals receiving USS (504 v. 474 (s.e. 21·1) g/day, P < 0·005) and higher in animals receiving barley grain and sugar-beet pulp than in those receiving grass hay (512 and 496 v. 370 (s.e. 25·9) g/day, P < 0·005). DOM intake also increased with the level of supplementation and this increase was greater with barley grain (504 to 634 and 314 to 554 g/day for ATS and USS) and sugar-beet pulp (440 to 582 and 315 to 522 g/day) than with grass hay (430 to 407 and 267 to 370 for ATS and USS). Urinary excretions of hypoxanthine, xanthine and uric acid were not affected by the experimental treatment whereas allantoin excretion (y, mmol) increased in response to DOM intake (x, kg) (y = 13·72 × − 0·26; r = 0·79; P < 0·001; no. = 96). The response in allantoin excretion was mainly explained by the increase in DOM intake. However when data were expressed per unit of DOM intake significant differences were still evident. Allantoin/DOM intake (mmol·kg) ratio and calculated microbial nitrogen (g·kg DOM intake) supply were lower with USS diets and sugar-beet pulp supplemented diets (P< 0·05) and increased significantly with level of supplementation (P < 0·001).