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6 - Pathogenicity islands and virulence of Salmonella enterica

Published online by Cambridge University Press:  04 December 2009

Duncan Maskell
By
Michael Hensel
Edited by
Pietro Mastroeni
Duncan Maskell
Affiliation:
University of Cambridge
Michael Hensel
Affiliation:
Institut für Klinische Mikrobiologie, Immunologie und Hygiene, Universität Erlangen-Nuemberg, Wasserturmstr. 3-5, D-91054 Erlangen, Germany
Pietro Mastroeni
Affiliation:
University of Cambridge
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Summary

INTRODUCTION

Horizontal gene transfer and bacterial virulence

Bacteria show a remarkable ability to adapt rapidly to new habitats. This observation also applies to pathogenic bacteria that have evolved strategies to colonize various anatomical niches of their multi-cellular hosts. The acquisition of genetic material by a process termed horizontal gene transfer is considered to be a driving force for the rapid evolution of bacteria as pathogens. Extrachromosomal DNA such as plasmids conferring resistance to antibiotics were the first horizontally transferred DNA elements to be identified, but later it became obvious that there are also mechanisms that allow the horizontal transfer of chromosomal DNA elements. The observation that certain virulence functions are clustered in distinct regions of the chromosome and that these regions are genetically unstable and were deleted with high frequencies gave the first clue about the existence of the new form of genetic elements. The term ‘Pathogenicity Island’ (PAI) was first introduced in 1983 by Hacker and colleagues who observed genetic instability of genes associated with the haemolytic activity of uropathogenic strains of Escherichia coli (Hacker et al., 1983). PAI were initially defined as large, unstable regions of the chromosome. With the identification of a large number of additional PAIs in various groups of bacterial pathogens, further common features were found.

Definition of pathogenicity islands

The following common characteristics were defined for PAIs:

  • They are large, distinct genetic entities on the bacterial chromosome.

  • They harbor one or more virulence genes.

  • They are often genetically unstable. This feature correlates with the presence of genetic elements involved in DNA-mobility, such as direct repeats, integrases, transposases and bacteriophage genes.

  • […]

Type
Chapter
Information
Salmonella Infections
Clinical, Immunological and Molecular Aspects
 , pp. 146 - 172
Publisher: Cambridge University Press
Print publication year: 2006

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