Brugia pahangi microfilariae obtained from peritoneal washings of jirds (Meriones unguiculatus) were incubated with midgut tissue of Culex quinquefasciatus and Aedes aegypti (susceptible stock), in the presence or absence of blood and also in thoracic tissue and salivary glands in various media. Exsheathment took place in the presence of midgut tissue with or without blood and in thoracic tissue, but not in the presence of salivary glands.
Percentage exsheathment was variable and not reproducible for each type of tissue. Exsheathment also occurred in the presence of mosquito cells in suspension and in monolayers. In the latter, percentage exsheathment in some cases was higher than that observed in vivo in the midgut of C. quinquefasciatus, which is refractory to B. pahangi microfilariae.
