Field experiment to assess the impact of radiation, temperature and foliar N application on rice was conducted. The treatments comprised of four sunlight levels, [control, 50% intensity during start to maximum tillering (R15–45), maximum tillering to booting (R46–75) and panicle emergence to maturity (R76–105) corresponding to 15–45, 46–75 and 76–105 days after transplanting] and 5 levels of foliar nitrogen [control, spray of 3% urea solution in water before (NB), midway (NM), afterwards (NA) and midway + afterwards (NMA) reduction in sunlight]. Results showed that leaf chlorophyll had an inverse relationship with radiation intensity. The R46–75 significantly reduced effective tillers (13.1–16.4%), R46–75 and R76–105 reduced grains/panicle (7.15–12.5%) as compared to control. NB produced significantly higher effective tillers (21.9–24.7%) and grains/panicle (12.2–12.9%) as compared to control. The reduction in sunlight and application of foliar nitrogen increased the minimum cooking time and decreased elongation ratio. Averaged over locations, R15–45, R46–75 and R76–105 decreased the yield significantly as compared to control by 9.29–11.3, 14.4–16.3 and 8.17–10.6%, respectively. The NB significantly increased grain yield as compared to control by 10.3% (Ludhiana) and 9.45% (Hoshiarpur). A decrease in maximum temperature (Tmax) by 2.85–5.70% (1–2°C) of 35.1°C, at 1416 μmol/m2/s of photosynthetically active radiation (PAR) increased rice productivity by 10.6–21.0%, while a similar decrease in PAR by 2.85–5.70% at a Tmax of 35.1°C, decreased the productivity by 2.05–4.10%. So, decrease in Tmax due to cloudy weather might have a positive influence while negative impact of deficit radiation may be mitigated by foliar application of 3% urea prior to/during the cloudy weather.

After seven generations of self-crossing assisted by β-glucuronidase (GUS) detection, 26 homozygous transgenic rice lines were obtained. Transgene effects on iron content, agronomic traits, rice quality and stress tolerance were studied. The results indicated that the average iron content in the milled grain of the transgenic (pea ferritin, Fer) rice (Oryza sativa) was 10.37 μg/g among all the homozygous lines, and only four lines (Fer34, Fer36, Fer39 and Fer65) had significantly higher iron contents than that of the control, Xiushui11 (6.46 μg/g). Significant differences in main agronomic traits, such as days from sowing to heading, plant height, main panicle length, total grains of main panicle, seed setting rate, 1000-grain weight, and yield per plant were observed among some homozygous lines, but similar flag leaf length and number of panicles per plant were found. This showed that insertion of the foreign gene (Fer) had a slight impact on the main agronomic traits, but that the transgene had no negative effects. The quality traits of homozygous rice lines, such as the percentage of brown rice, milled rice and full milled rice, grain length, grain width, ratio of grain length and width, and amylose content were all similar to those of the control. However, variations were noticed in chalkiness, translucency, alkali spreading value and gel consistency among partial homozygous lines. Responses of homozygous lines to low and high temperatures were not significantly correlated with the iron contents, and resistance to rice blast (Pyricularia oryzae), bacterial blight (Xanthomonas oryzae) and brown planthopper (Niaparvata lugens) were enhanced with increased iron contents.