Hostname: page-component-586b7cd67f-l7hp2 Total loading time: 0 Render date: 2024-11-25T23:04:51.907Z Has data issue: false hasContentIssue false
  • English
  • Français

Variation in biological characteristics of purified pea ferritin (Fer) transgenic rice

Published online by Cambridge University Press:  13 February 2008

Ye Hong-Xia ,
Guo Ze-Jian ,
Li Mei ,
Xu Xiao-Hui ,
Bao Jin-Song  and
Shen Sheng-Quan
Ye Hong-Xia
Affiliation:
Institute of Nuclear Agricultural Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310029, China
Guo Ze-Jian
Affiliation:
Department of Plant Pathology, China Agricultural University, Beijing, 100094, China
Li Mei
Affiliation:
Institute of Nuclear Agricultural Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310029, China
Xu Xiao-Hui
Affiliation:
College of Life Science, China Jiliang University, Hangzhou, 310018, China
Bao Jin-Song
Affiliation:
Institute of Nuclear Agricultural Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310029, China
Shen Sheng-Quan*
Affiliation:
Institute of Nuclear Agricultural Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310029, China
*
*Corresponding author. E-mail: [email protected]
Get access Rights & Permissions [Opens in a new window]

Abstract

After seven generations of self-crossing assisted by β-glucuronidase (GUS) detection, 26 homozygous transgenic rice lines were obtained. Transgene effects on iron content, agronomic traits, rice quality and stress tolerance were studied. The results indicated that the average iron content in the milled grain of the transgenic (pea ferritin, Fer) rice (Oryza sativa) was 10.37 μg/g among all the homozygous lines, and only four lines (Fer34, Fer36, Fer39 and Fer65) had significantly higher iron contents than that of the control, Xiushui11 (6.46 μg/g). Significant differences in main agronomic traits, such as days from sowing to heading, plant height, main panicle length, total grains of main panicle, seed setting rate, 1000-grain weight, and yield per plant were observed among some homozygous lines, but similar flag leaf length and number of panicles per plant were found. This showed that insertion of the foreign gene (Fer) had a slight impact on the main agronomic traits, but that the transgene had no negative effects. The quality traits of homozygous rice lines, such as the percentage of brown rice, milled rice and full milled rice, grain length, grain width, ratio of grain length and width, and amylose content were all similar to those of the control. However, variations were noticed in chalkiness, translucency, alkali spreading value and gel consistency among partial homozygous lines. Responses of homozygous lines to low and high temperatures were not significantly correlated with the iron contents, and resistance to rice blast (Pyricularia oryzae), bacterial blight (Xanthomonas oryzae) and brown planthopper (Niaparvata lugens) were enhanced with increased iron contents.

Keywords

transgenic riceferritinagronomic traitsrice qualitystress tolerance
Type
Research Article
Information
Chinese Journal of Agricultural Biotechnology , Volume 4 , Issue 3 , December 2007 , pp. 213 - 219
Copyright
Copyright © China Agricultural University and Cambridge University Press 2007

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

First published in Journal of Agricultural Biotechnology 2007, 15(2): 251–256

References

Andrews, S (1992) Structure, function and evolution of ferritins. Journal of Inorganic Biochemistry 47(1): 161–174.CrossRefGoogle ScholarPubMed
Briat, JF (1996) Roles of ferritin in plants. Journal of Plant Nutrition 19(10): 1331–1342.CrossRefGoogle Scholar
Briat, JF and Lobreaux, S (1997) Iron transport and storage in plants. Trends in Plant Science 2(5): 187–193.CrossRefGoogle Scholar
Cheng, ZQ, Guo, ZJ, Xu, XH, Cai, RY and Li, DB (2003) Ferritin transgenic rice plants are tolerant to oxidative stress and Magnaporthe grisea infection. Chinese Journal Rice Science 17(1): 85–88 (in Chinese with English abstract).Google Scholar
Goto, F, Yoshihara, T and Shigemoto, N (1999) Iron fortification of rice seed by the soybean ferritin gene. Nature Biotechnology 17(3): 282–286.CrossRefGoogle ScholarPubMed
Goto, F, Yoshihara, T and Saiki, H (2000) Iron accumulation and enhanced growth in transgenic lettuce plants expressing the iron-binding protein ferritin. Theoretical and Applied Genetics 100: 658–664.CrossRefGoogle Scholar
Liu, QQ, Yao, QH and Wang, HM (2004) Endosperm-specific expression of the ferritin gene in transgenic rice (Oryza sativa L.) results in increased iron content of milling rice. Acta Genetica Sinica 31(5): 518–524 (in Chinese with English abstract).Google ScholarPubMed
Lucca, P, Hurrell, R and Potrykus, I (2001) Genetic engineering approaches to improve the bioavailability and the level of iron in rice grains. Theoretical and Applied Genetics 102(3): 392–397.CrossRefGoogle Scholar
Meng, FH and Wei, YZ (2004) Advance of research on the content and bioavailability of iron in rice. Journal of Northwest Sci-tech University of Agricultural and Forestry 32(2): 73–77 (in Chinese with English abstract).Google Scholar
Murray, MG and Thompson, WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research 8(19): 4321–4325.CrossRefGoogle ScholarPubMed
Ren, XL (2005) Grain quality and developmental characteristics and related molecular genetical analysis of low phytate mutant rice (Oryza sativa L.). Ph.D. dissertation, Zhejiang University (in Chinese with English abstract).Google Scholar
Rueb, S and Hensgens, LAM (1989) Improved histochemical staining for β-D-glucuronidase activity in monocotyledonous plants. Rice Genetics Newsletter 6: 168–169.Google Scholar
Shen, SQ, Shu, QY, Bao, JS, Wu, DX and Xix, YW (2005) Study on the effect Bt gene on agronomic traits of rice using near isogenic lines. Journal of Zhejiang University (Agriculture and Life Sciences) 31(3): 283–287 (in Chinese with English abstract).Google Scholar
Shen, ZT (1995) Crop Breeding Experimentation. Beijing: China Agriculture Publishing Company.Google Scholar
Vasconcelos, M, Datta, K, Oliva, N, et al. (2003) Enhanced iron and zinc accumulation in transgenic rice with the ferritin gene. Plant Science 164: 371–378.CrossRefGoogle Scholar
Xu, XH, Guo, ZJ and Cheng, ZQ (2003) Introduction of ferritin gene into rice and the functional analysis of transgenic plants. Journal of Zhejiang University (Agriculture and Life Sciences) 29(1): 49–54 (in Chinese with English abstract).Google Scholar