Currently, a limited number of herbicides is available to treat water-seeded rice in California, with widespread resistance to most of those herbicides. Because no resistant grasses showed resistance to pendimethalin, a series of studies were conducted to evaluate water-seeded rice response to pendimethalin. In a field study conducted at the Rice Experiment Station at Biggs, California, in 2020 and 2021, three pendimethalin formulations, a granule (GR), emulsifiable concentrate (EC), and capsule suspension (CS), were applied at 1.1, 2.3, and 3.4 kg ai ha−1 rates, and at 5, 10, and 15 d after seeding onto water-seeded rice. In addition, a greenhouse study was conducted to examine the response of five common California rice cultivars to GR and CS formulation applications. Echinochloa control levels were reduced at 15 d after seeding after use of EC and CS formulations compared with earlier timings. In both years, rice grain yields were increased by 3,014 kg ha−1 after application of pendimethalin at 3.4 kg ai ha−1 when applied at 15 d after seeding compared with 5 and 10 d after seeding, and similar to 1.1 kg ai ha−1 applications. The GR and CS were safer formulations based on a reduction in injury and an increase in grain yields compared to the EC formulation. Differences in seedling vigor across cultivars appeared to incur an advantage after a pendimethalin application. However, most cultivars evaluated for stand reduction and dry biomass demonstrated tolerance to GR and CS formulation applications only after rice reached the 3-leaf stage. In contrast, an application at 1-leaf stage rice reduced stand up to 68%. Application rate, timing, and formulation are important factors to consider if the use of pendimethalin in water-seeded rice is to be pursued.

Quizalofop-resistant rice allows for over-the-top applications of quizalofop, a herbicide that inhibits acetyl-coenzyme A carboxylase. However, previous reports have indicated that quizalofop applied postemergence may cause significant injury to quizalofop-resistant rice. Therefore, field experiments were conducted to evaluate the response of quizalofop-resistant rice cultivars to quizalofop applications across different planting dates. Under controlled conditions, the effects of soil moisture content, air temperature, and light intensity on quizalofop-resistant rice sensitivity to quizalofop were investigated. In the planting date experiment, injury of more than 11 percentage points was observed on early-planted rice compared with late-planted rice at the 5-leaf stage, with higher injury observed under saturated soil conditions. However, quizalofop applications at the labeled rate caused ≤16% reduction in yield regardless of planting environment. Quizalofop-resistant cultivars exhibited more injury by at least 25 percentage points when soil was maintained at 90% or 100% of field capacity because rice cultivars ‘PVL01’, ‘PVL02’, and ‘RTv7231 MA’ exhibited ≥42%, 30%, and ≥54% injury, respectively, compared with ≤10%, ≤5%, and ≤22% injury, respectively, at 40% or 50% of field capacity, pooled over rating timing. Greater injury ranging from 18% to 31% was observed on quizalofop-resistant rice grown under low light intensity (600 µmol m−2s−1) compared with 5% to 14% injury under high light intensity (1,150 µmol m−2s−1). The injury persisted from 7 to 28 d after 5-leaf stage application (DAFT), averaged over quizalofop-resistant cultivars and air temperatures (20/15 C and 30/25 C day/night, respectively). At 7 DAFT, greater injury (by 5 to 21 percentage points) was observed on quizalofop-resistant cultivars; PVL01, PVL02, and RTv7231 MA exhibited 33%, 9%, and 58% injury, respectively, under 20/15 C temperature conditions compared with 13%, 4%, and 37% injury, respectively, under 30/25 C day/night conditions averaged over light intensities. Overall, quizalofop is likely to cause a greater risk for injury to quizalofop-resistant rice if it is applied under cool, cloudy, and moist soil conditions.

Injury to quizalofop-resistant rice was reported in some fields following postemergence applications of quizalofop. Glyphosate-resistant (GR) corn, cotton, and soybean, and imidazolinone-resistant rice are grown near quizalofop-resistant rice. Herbicide drift from glyphosate and imazethapyr and the resulting crop injury and potential yield loss is a cause of concern for producers. Field experiments conducted near Colt, and Keiser, AR, in 2021 evaluated whether low rates of glyphosate or imazethapyr interact with sequential quizalofop applications to exacerbate injury to quizalofop-resistant rice compared to quizalofop applications alone. Herbicide treatments consisted of a low rate of glyphosate (90 g ae ha−1) or imazethapyr (10.7 g ai ha−1) applied 10, 7, 4, and 0 d before the 2-leaf growth stage of rice, and glyphosate or imazethapyr, at the same rate and timings, followed by quizalofop at 120 g ai ha−1 applied to 2-leaf rice. All plots treated with quizalofop received a subsequent application of the same herbicide and rate at the 5-leaf rice stage. At 28 d after final treatment (DAFT), glyphosate followed by quizalofop the same day to 2-leaf rice caused 77% injury compared with 58% when glyphosate was applied alone, regardless of location. Glyphosate followed by quizalofop the same day reduced rough rice grain yield by 67% compared with 33% when glyphosate was applied alone to 2-leaf rice at the Colt location. Application of imazethapyr followed by quizalofop the same day to 2-leaf rice caused more injury (63% and 19% injury at the Colt and Keiser locations, respectively) than imazethapyr alone (42% and 7% injury at the Colt and Keiser locations, respectively) at 35 DAFT. Overall, glyphosate and imazethapyr followed by quizalofop applications worsened injury compared to glyphosate, imazethapyr, and quizalofop applications alone. As the interval between exposure to a low rate of glyphosate or imazethapyr and quizalofop decreases, the detrimental effect of herbicide on rice likewise increases.

Environmental conditions surrounding herbicide applications are known to affect weed control and crop response. Variable levels of rice injury caused by florpyrauxifen-benzyl have been observed across cropping systems and environmental conditions, warranting research in which single environmental and management strategies are isolated to understand the effect of each factor on rice injury and subsequent reductions in rice growth. A field study was conducted to determine the effects of planting date, rice cultivar, and florpyrauxifen-benzyl rate on rice injury, maturity, and yield. Two greenhouse studies were conducted to determine the effect of soil moisture and time of flooding after florpyrauxifen-benzyl application on rice injury caused by the herbicide. Growth chamber experiments were conducted to isolate the effects of temperature and light intensity on rice injury caused by florpyrauxifen-benzyl. In the field study, levels of injury varied across planting dates in both years, indicating the influence of environment on the crop response to florpyrauxifen-benzyl applications. Under dry (40% soil moisture) and saturated (100%) soil conditions, rice injury increased to 36% and 35%, respectively, compared with 27% and 25% injury at 60% and 80% soil moisture, respectively. Flooding rice 0 to 6 d after florpyrauxifen-benzyl application reduced visible injury; however, a reduction in rice tiller production occurred when the rice was flooded the same day as application. Visible rice injury increased when florpyrauxifen-benzyl was applied under low light intensity (700 µmol m−2 s−1) and high temperatures (35/24 C day/night). Based on these findings, applications of florpyrauxifen-benzyl are least likely to cause unacceptable rice injury when applied to soils having 60% and 80% saturation in high light, low temperature environments, and the crop is flooded 3 to 6 d following application.

Field experiments were initiated near Colt, AR, in the fall of 2016 and continued through the summer of 2018 to evaluate rice tolerance and weedy (or red) rice control after fall-applied very-long-chain fatty acid (VLCFA)-inhibiting herbicides. A split-plot design was used for the experiment, with the whole-plot factor being winter condition (flooded or non-flooded) and the split-plot factors being herbicide and rate. Herbicide treatments included acetochlor, dimethenamid-P, pethoxamid, pyroxasulfone, and S-metolachlor applied at 1,050, 525, 420, 205, and 1,070 g ai ha−1 and at 2,100, 1,050, 840, 410, and 2,140 g ha−1 for low rates and high rates, respectively. Herbicides were applied in the fall, then ‘CL172’ rice was drill seeded in the spring of the following calendar year. Weedy rice control differed between years, but acetochlor and pyroxasulfone consistently provided the greatest levels of control across rates and flood conditions. Consequently, herbicides that best controlled weedy rice also caused the greatest injury to cultivated rice. Rice injury did not exceed 13% regardless of herbicide treatment at 3 wk after planting (WAP). However, the high rate of pyroxasulfone caused 20% rice injury at 5 WAP in 2018. Although it was expected that winter condition may affect residual activity of the VLCFA-inhibiting herbicides, herbicide selection and application rate both had much greater effects on rice injury and on weedy rice control. Based on these results, rice injury would be minimal or nonexistent after fall applications of the tested VLCFA inhibitors, and intermediate levels of weedy rice control may be achieved. The implementation of VLCFA-inhibiting herbicides in rice production systems would offer a novel herbicide site of action and offer a degree of selective control of weedy rice.

Each year there are multiple reports of drift occurrences, and the majority of drift complaints in rice are from imazethapyr or glyphosate. In 2014 and 2015, multiple field experiments were conducted near Stuttgart, AR, and near Lonoke, AR, to evaluate whether insecticide seed treatments would reduce injury from glyphosate or imazethapyr drift or decrease the recovery time following exposure to a low rate of these herbicides. Study I was referred to as the “seed treatment study,” and Study II was the “drift timing study.” In the seed treatment study the conventional rice cultivar ‘Roy J’ was planted, and herbicide treatments included imazethapyr at 10.5 g ai ha–1, glyphosate at 126 g ae ha–1, or no herbicide. Each plot had either a seed treatment of thiamethoxam, clothianidin, chlorantraniliprole, or no insecticide seed treatment. The herbicides were applied at the two- to three-leaf growth stage. Crop injury was assessed 1, 3, and 5 wk after application. Averaged over site-years, thiamethoxam-treated rice had less injury than rice with no insecticide seed treatment at each rating, along with an increased yield. Clothianidin-treated rice had an increased yield over no insecticide seed treatment, but the reduction in injury for both herbicides was less pronounced than in the thiamethoxam-treated plots. Overall, chlorantraniliprole was generally the least effective of the three insecticides in reducing injury from either herbicide and in protecting rice yield potential. A second experiment conducted at Stuttgart, AR, was meant to determine whether damage to rice from glyphosate and imazethapyr was influenced by the timing (15, 30, and 45 d after planting) of exposure to herbicides for thiamethoxam-treated and nontreated rice. There was an overall reduction in injury with the use of thiamethoxam, but the reduction in injury was not dependent on the timing of the drift event. Reduction in damage from physical drift of glyphosate and imazethapyr as well as increased yields over the absence of an insecticide seed treatment appear to be an added benefit.

Herbicide resistance to several of the most common weed species in US rice production, such as barnyardgrass and red rice, has made weed control extremely difficult with available herbicide options. No very-long-chain fatty acid–inhibiting herbicides are labeled for use in US rice; however, pethoxamid is one such herbicide under development for soil-applied use to control grasses and small-seeded broadleaves in rice and various row crops. Field trials were conducted in 2015 and 2016 near Stuttgart, AR, for rice tolerance and in 2016 near Colt, AR, and Lonoke, AR, for weed control with the use of pethoxamid-containing rice herbicide programs. Pethoxamid was applied alone and in a program at 420 and 560 g ai ha–1 with other herbicides labeled in rice including clomazone, quinclorac, propanil, imazethapyr, and carfentrazone POST. Injury less than 10% was seen for all treatments 2 wk after application in 2015 and 2016, except for pethoxamid at 420 g ha–1 to clomazone to one-leaf rice. Rice injury dissipated to less than 5% following all treatments by 4 wk after flood establishment. Barnyardgrass was controlled 95% or more near Colt and 93% or more near Lonoke for herbicide programs including clomazone PRE followed by pethoxamid plus quinclorac or imazethapyr at three- to four-leaf stage rice. Considering the minimal injury and high levels of barnyardgrass control associated with pethoxamid-containing weed control programs, pethoxamid provides a unique and effective site of action for use in US rice production.

Field experiments were conducted to compare efficacy of BAS 514, bromoxynil, fenoxaprop, sethoxydim, and triclopyr with standard herbicides for barnyardgrass and bearded sprangletop control in rice at four locations. Sequential applications of BAS 514 and fenoxaprop, or propanil and sethoxydim consistently controlled barnyardgrass and bearded sprangletop as well as or better than standard treatments of propanil, thiobencarb, or pendimethalin alone or combined; and rice so treated produced high yields. BAS 514 applied postemergence alone, BAS 514 applied sequentially with sethoxydim, or BAS 514 tank mixed with propanil controlled barnyardgrass but not bearded sprangletop. Bromoxynil or triclopyr tank mixed with fenoxaprop or sethoxydim antagonized activity on barnyardgrass and bearded sprangletop. Combinations of fenoxaprop with BAS 514 or propanil consistently provided barnyardgrass and bearded sprangletop control and high yields.

Greenhouse experiments were conducted to evaluate seedling rice tolerance to haloxyfop and to determine soil moisture effects on root, shoot, and root plus shoot uptake. Visible injury to rice at 4 wk after treatment increased with each increase of haloxyfop rate from 40 to 160 g ai/ha, regardless of growth stage. Less injury was observed at equivalent rates in four-leaf than in younger rice. Similarly, equivalent haloxyfop rates reduced shoot fresh weight of one- to three-leaf rice more than shoot fresh weight of four-leaf rice. There was no interation between soil moisture levels (19 and 24%) and haloxyfop activity. Fresh weights were less when haloxyfop at 40 or 80 g/ha was absorbed by both roots and shoots than when absorption was by either roots or shoots alone. However, at 80 g/ha fresh weight was similar whether haloxyfop was absorbed by roots or shoots alone. Root uptake, in the presence of 160 g/ha haloxyfop, did not contribute to reduced fresh weight with root plus shoot treatments because of the initial foliar activity incurred.

Barnyardgrass and bearded sprangletop are severe weed pests in rice in the southern United States. These weeds are controlled with standard herbicide programs of propanil alone or propanil combined with pendimethalin or thiobencarb. However, new herbicides, such as fenoxaprop, may be effective alternatives for grass weed control in rice. Fenoxaprop at 0.17 kg ai/ha applied postemergence in programs with propanil, thiobencarb, or pendimethalin controlled barnyardgrass and bearded sprangletop in rice and performed as well as or better than standard treatments of propanil with thiobencarb or pendimethalin. Rice yielded well, and grain was of high quality.

Field experiments were conducted in 1999 and 2000 at the Rice Research and Extension Center at Stuttgart, AR, on a DeWitt silt loam and at the Pine Tree Branch Experiment Station near Colt, AR, on a Calloway silt loam to evaluate the tolerance of 14 rice cultivars and four experimental cultivar lines to clomazone at 0.34 and 0.67 kg ai/ha applied preemergence. Early-season chlorosis 14 days after emergence (DAE) ranged from 1 to 21% in 1999 and 3 to 40% in 2000 when averaged over herbicide rate. The experimental cultivar line RU961096 was slightly more susceptible to clomazone than other cultivars and lines. However, RU9701041 was more tolerant to clomazone at 0.67 kg/ha 14 DAE than other cultivars and lines. By 28 DAE, all cultivars had <13% chlorosis in 1999 and <8% in 2000. The experimental cultivar line RU9601096 and cultivar Koshihikari did not recover as quickly as the other 16 cultivars and cultivar lines. Early-season chlorosis had no effect on days to 50% heading or yield for any of the cultivars evaluated.

Intensive selection pressure from repeated use of propanil and quinclorac led to the evolution of herbicide-resistant barnyardgrass biotypes. Twenty-two composite field samples were tested for level of resistance in 2002 and 2003, and field studies were conducted at the Rice Research and Extension Center, Stuttgart, AR, in 2002 and 2003 to evaluate alternative rice herbicides to control propanil-resistant (PR) and quinclorac-resistant (QR) barnyardgrass. Of the 22 composite samples, four were PR (30 to 40% control); four had a mixed population of PR, QR, and susceptible (S) barnyardgrass; and two had multiple resistance to propanil and quinclorac (P/QR), with control from propanil of 15 to 30% and control from quinclorac of 5 to 10%. ‘Wells’ rice was used where conventional herbicide programs were evaluated, and Clearfield rice ‘CL-161’ (imidazolinone-resistant) was used for herbicide programs involving imazethapyr. All PR and QR barnyardgrass were controlled > 90% by alternative herbicides, including all preemergence (PRE) and delayed preemergence (DPRE) treatments. By 56 d after emergence (DAE), cyhalofop or fenoxaprop applied to two- to three-leaf barnyardgrass (early postemergence [EPOST]), followed by (fb) a preflood application, controlled barnyardgrass > 93%. Pendimethalin controlled PR barnyardgrass 21 DAE, but not all season long. In contrast, imazethapyr in Clearfield rice controlled all grass weeds 100% all season long. Midpostemergence (MPOST) bispyribac application at the four- to five-leaf stage also provided season-long control of all barnyardgrass biotypes (> 88%, 56 DAE). Rice yields ranged from 5,300 to 5,700 kg ha−1 in conventional weed-control treatments and from 2,800 to 5,000 kg ha−1 in imazethapyr-treated plots. Nontreated plots yielded 1,500 kg ha−1.

Greenhouse research was conducted to evaluate shoot and root growth response of imidazolinone-tolerant (IT) rice cultivars to imazethapyr applied postemergence at various rates and application timings. Imazethapyr was applied at 70, 140, and 280 g ai/ha to IT cultivars ‘CL 121’ and ‘CL 161’ in the one- to two-leaf and three- to four-leaf growth stages. Imazethapyr applied to one- to two-leaf or three- to four-leaf rice at 70, 140, and 280 g/ha was more injurious to CL 121 than to CL 161. At 3 wk after treatment (WAT), CL 121 was injured 23 to 38% regardless of application timing. In contrast, CL 161 was injured no more than 11% at 3 WAT. Shoot:root ratio for CL 161 was not affected by imazethapyr application. For CL 121, shoot:root ratio was lower following imazethapyr at 280 g/ha than at 70 or 140 g/ha. Based on shoot fresh weight following imazethapyr at 70 g/ha, CL 161 was 1.8 times more tolerant than CL 121 at 2 WAT and 1.3 times more tolerant at 3 WAT. The IT rice cultivar CL 161 is inherently more tolerant to imazethapyr than is CL 121 based on visual injury and shoot and root growth.