Horsenettle was described quantitatively in two grazed bermudagrass pastures for two years. Six times from April through October, plants were counted, phenologically documented, and tops were harvested for estimation of above-ground phytomass. Roots were sampled for carbohydrate, N fraction, and macronutrient analyses. Top growth, flowering, fruiting, and chemical composition were similar between sites within years, but differed between years. Starch and total non-structural carbohydrates in roots increased following reproductive periods both years. Root concentrations of protein and non-protein N increased and concentrations of P decreased in August and September in one year only. Analysis of the relationship between seasonal trends in carbohydrate fluctuation and pasture management practices suggests that systemic herbicide application in mid-summer may lead to more effective control than application in spring.