Khakiweed is a perennial broadleaf weed that is difficult to control because of its multiple means of reproduction, vigorous growth, and deep taproot. Khakiweed reduces the performance of pasture, pecan, and turf areas by choking out desirable grass and legume species. Because information on the effectiveness of contact and residual herbicides for control in pecan and pasture areas is limited, greenhouse studies were conducted to determine the effect of application timing, mode of action, and rate on khakiweed control. Preemergence and postemergence herbicides were applied to mature khakiweed plants at 0.25X, 0.5X, 1X, or 2X the label recommended rate for general broadleaf control. Biomass was collected 3 wk after application. Plants regrew from roots in the greenhouse until a second biomass harvest was collected at 6 wk after treatment (WAT). Metsulfuron-methyl, indaziflam, or pendimethalin was applied preemergence to the soil surface. All rates of preemergence herbicides provided high-efficacy control of regrowth (>85%) compared to the nontreated control. The efficacies of postemergence-applied metsulfuron-methyl, metsulfuron-methyl + nicosulfuron, indaziflam, aminopyralid + florpyrauxifen-benzyl, 2,4-D amine, and 2,4-D amine + florpyrauxifen-benzyl were also examined. All postemergence herbicide treatments exhibited control compared to the nontreated plants at both sample timings (3 and 6 WAT) and increased with herbicide application rate. No herbicide provided high-efficacy control during the initial postspray period (0 to 3 WAT). During the regrowth period (3 to 6 WAT), metsulfuron-methyl alone and in combination gave >85% control of khakiweed biomass, indicating that the sulfonylurea herbicides used in this study are well suited to controlling khakiweed.

Rice herbicide drift poses a significant challenge in California, where rice fields are near almond, pistachio, and walnut orchards. This research was conducted as part of a stewardship program for a newly registered rice herbicide and specifically aimed to compare the onset of foliar symptoms resulting from simulated florpyrauxifen-benzyl drift with residues in almond, pistachio, and walnut leaves at several time points after exposure. Treatments were applied to one side of the canopy of 1- and 2-yr-old trees at 1/100X and 1/33X of the florpyrauxifen-benzyl rice field use rate of 29.4 g ai ha–1 in 2020 and 2021. Symptoms were observed 3 d after treatment (DAT) for pistachio and 7 DAT for almond and walnut, with peak severity at approximately 14 DAT. While almond and walnut symptoms gradually dissipated throughout the growing season, pistachio still had symptoms at leaf out in the following spring. Leaf samples were randomly collected from each tree for residue analysis at 7, 14, and 28 DAT. At 7 DAT with the 1/33X rate, almond, pistachio, and walnut leaves had florpyrauxifen-benzyl at 6.06, 5.95, and 13.12 ng g–1 fresh weight (FW) leaf, respectively. By 28 DAT, all samples from all crops treated with the 1/33X drift rate had florpyrauxifen-benzyl at less than 0.25 ng g–1 FW leaf. At the 1/100X rate, pistachio, almond, and walnut residues were 1.78, 2.31, and 3.58 ng g–1 FW leaf at 7 DAT, respectively. At 28 DAT with the 1/100X rate, pistachio and almond samples had florpyrauxifen-benzyl at 0.1 and 0.04 ng g–1 FW leaf, respectively, but walnut leaves did not have detectable residues. Together, these data suggest that residue analysis from leaf samples collected after severe symptoms may substantially underestimate actual exposure due to the relatively rapid dissipation of florpyrauxifen-benzyl in nut tree foliage.

Complaints regarding the sensitivity of rice to florpyrauxifen-benzyl and off-target movement of the herbicide occurred following its commercial launch in 2018 in the midsouthern United States. These two concerns encouraged the exploration of an alternative application method for florpyrauxifen-benzyl in rice. A field study was conducted in 2020 and 2021 to determine if coating florpyrauxifen-benzyl on urea would reduce negative impacts of the herbicide to rice. Five commercial rice lines were evaluated: ‘Diamond’, ‘Titan’, ‘RT7321 FP’, ‘RT7521 FP’, and ‘XP753’. Florpyrauxifen-benzyl coated on urea at a 2× rate (60 g ai ha–1) reduced rice injury in one of five commercial rice lines in 2020 and four of five commercial rice lines in 2021, compared to spray applications at the same rate. In 2020, ‘RT7521 FP’ exhibited a 17-percentage point injury reduction when coating florpyrauxifen-benzyl on urea at a 2× rate vs. the same rate sprayed. In 2021, rice injury was reduced by 26, 10, and 27 percentage points in the commercial rice lines ‘Diamond’, ‘Titan’, and ‘XP753’, respectively, following coated urea vs. spray applications at 4 wk after treatment (WAT). ‘XP753’ exhibited reduced injury (15 percentage points) by coating florpyrauxifen-benzyl at a 1× rate (30 g ai ha–1) 4 WAT in 2021, and another, ‘Diamond’, had comparable groundcover to nontreated plots when florpyrauxifen-benzyl was coated on urea at a 1× rate rather than the reductions observed from the spray application at a 2× rate. Yield differences were a function of urea rate rather than application method, where in six out of ten instances greater rough rice grain yield occurred at the higher rate. Findings from this experiment indicate that coating florpyrauxifen-benzyl on urea can reduce the amount of injury observed, especially in areas of overlap where you would have a 2× rate.

Florpyrauxifen-benzyl has generated complaints and concerns around rice injury and off-target movement to soybean since its commercial launch in 2018. Developing a precise method for applying florpyrauxifen-benzyl was imperative for its continued use. Experiments were conducted in 2020 and 2021 to evaluate rice weed control as influenced by preflood application interval and flood loss following florpyrauxifen-benzyl at 30 g ai ha−1 applied as a spray or coated on urea. In a preflood application experiment, coating florpyrauxifen-benzyl on urea and applying it the day of flood establishment and 5 and 10 d prior to flooding (DPTF) resulted in lower yellow nutsedge, broadleaf signalgrass, and barnyardgrass control than when the herbicide was spray at 3 and 5 wk after final treatment (WAFT). Coating florpyrauxifen-benzyl onto urea provided only 61% to 63% yellow nutsedge control at 3 and 5 WAFT, which was 35 to 37 percentage points lower than when the spray was applied at 5 or 10 DPTF. Likewise, rice yields following applications of florpyrauxifen-benzyl coated onto urea were 1,200 kg ha−1 less than yields following spray applications. Florpyrauxifen-benzyl coated onto urea and clomazone provided lower levels of weed control than spraying the herbicide alone, suggesting an explanation for the yield losses. The timing of flood loss experiment suggested that when florpyrauxifen-benzyl coated onto urea at 30 g ai ha−1 was applied preflood and flood was relinquished at 2 h, 24 h, and 7 d after flood establishment, hemp sesbania and yellow nutsedge control were not affected. However, loss of floodwater 2 h after flood establishment resulted in lower barnyardgrass control than when the flood was lost 24 h and 7 d after flooding. Generally, the period between a herbicide application and flooding completion should be minimized to aid in weed control. These results indicate the importance of maintaining a flood for weed control and nutrient management.

Rice in Mississippi is often in early seedling growth stages when paraquat-based herbicide treatments are commonly applied to corn, cotton, and soybean; therefore, off-target movement of the herbicide onto adjacent rice fields may occur. After an off-target movement event has occurred, weed management in the rice crop is still necessary. Field studies were conducted from 2019 to 2021 in Stoneville, MS, to evaluate rice injury and barnyardgrass control with labeled herbicides after exposure to a sublethal concentration of paraquat. Herbicide treatments were label-recommended rates of imazethapyr, quinclorac, propanil, bispyribac-sodium, cyhalopfop, and florpyrauxifen-benzyl applied following rice exposure to a sublethal concentration of paraquat. Rice injury was detected 7 and 28 d after treatment (DAT) and was ≥35% and ≥14%, respectively, for all herbicides. Florpyrauxifen-benzyl and imazethapyr caused the greatest rice injury at 28 DAT. Following paraquat exposure, barnyardgrass control was similar for all labeled herbicide treatments at 7, 14, and 28 DAT except for florpyrauxifen-benzyl and no herbicide (paraquat alone) at 7 DAT. Across all evaluations, barnyardgrass control was at least 12% greater following paraquat exposure and labeled herbicide treatments than with no paraquat exposure. The current research demonstrates that labeled rates of herbicides applied following exposure to a sublethal concentration of paraquat resulted in <36% injury and provided as great as 95% control of barnyardgrass, depending on the herbicide treatment. Therefore, the labeled herbicides choice following rice exposure to a sublethal concentration of paraquat should be based on weed spectrum.

Florpyrauxifen-benzyl was commercialized in 2018 to target barnyardgrass and aquatic or broadleaf weeds. Field studies were conducted from 2019 to 2021 in Stoneville, MS, to evaluate barnyardgrass control following a simulated failure of florpyrauxifen-benzyl or other common postemergence rice herbicides. In the first field study, florpyrauxifen-benzyl was applied at 0 and 15 g ai ha–1 to rice at the two- to three-leaf stage to simulate a failed application targeting barnyardgrass. Sequential herbicide treatments included no herbicide and full rates of imazethapyr, quinclorac, bispyribac-Na, and cyhalofop applied 7 or 14 d after florpyrauxifen-benzyl treatment. The second field study was designed to evaluate barnyardgrass control with florpyrauxifen-benzyl following simulated failure of postemergence rice herbicides. Initial herbicide treatments included no herbicide and half rates of imazethapyr, quinclorac, bispyribac-Na, and propanil. Sequential applications at 7 or 14 d after the initial herbicide treatments included florpyrauxifen-benzyl at 0 and 30 g ai ha–1. Results from the first study indicated barnyardgrass control 21 d after final treatment (DAFT) was greater with sequential treatments at 7 compared with 14 d after initial treatment (DA-I) with no initial application of florpyrauxifen-benzyl. Therefore, delaying sequential treatments until 14 d after initial florpyrauxifen-benzyl at 15 g ha–1 allowed barnyardgrass to become too large to control with other rice herbicides. Rough rice yield was reduced in plots where quinclorac application was delayed from 7 to 14 DA-I with no initial application of florpyrauxifen-benzyl. The second study suggested that florpyrauxifen-benzyl application should be delayed 14 d after a herbicide failure. Although no differences in barnyardgrass control 21 DAFT were detected whether florpyrauxifen-benzyl was applied 7 or 14 DA-I of any herbicide utilized, >85% control was only achieved when florpyrauxifen-benzyl application was delayed 14 DA-I. These results demonstrate barnyardgrass control options following simulated failed applications of common rice herbicides.

The evolution of herbicide resistance in weeds can reduce the herbicide’s efficacy, depleting crop yield and quality. Our group previously confirmed 2,4-D resistance in three Palmer amaranth (Amaranthus palmeri S. Watson) populations (R1 to R3). In the current study, the first filial (F1) seeds of 2,4-D–resistant populations were subjected to screening tests for resistance to other auxin-mimicking herbicides, florpyrauxifen-benzyl (FPB; 30 g ai ha−1) and dicamba (560 g ae ha−1). Dicamba killed all resistant populations. FPB provided 100% control of only the R3 population. Sensitivities to FPB were reduced by 2 and 35 percentage points in R1 and R2 populations, respectively. Pretreatment with malathion increased FPB sensitivity by 15 percentage points in the R2 population. FPB resistance characterization and mechanism were evaluated using a purified line of the R2 population (F2). The FPB sensitivity was 29-fold lower in the F2 line than in the susceptible (S) standard. Absorption, translocation, and total metabolism of FPB were similar for S and R2 populations. However, less florpyrauxifen-acid (FPA) was detected in the R2 population (17.0% to 25.4%) than in the S population (22.8% to 33.2%), due to its rapid metabolism and/or reduced production with resistance evolution. Because the results of the non–target site resistance mechanism evaluation observed in this study were insufficient to account for the 29-fold reduced sensitivity of the R2 population to FPB, further genetic studies are needed to investigate the presence of target-site resistance in that population.

Unoccupied aerial application systems (UAAS) are gaining popularity for weed management to increase applicator safety and to deliver herbicide treatments where treatment sites limit ground-based spray equipment. Several studies have documented UAAS application strategies and procedures for weed control in terrestrial settings, yet literature describing remote spray technology for use in aquatics remains limited. Currently, applicators seek guidance for UAAS deployment for aquatic weed management to overcome site access restrictions, deal with environmental limitations, and improve ground-based applicator safety in hazardous treatment scenarios. In the present case studies, we evaluate a consumer-available UAAS to deliver the herbicide, florpyrauxifen-benzyl, as both foliar and directed in-water spray applications. The first case study showed that the invasive floating-leaved plant, yellow floating heart, was controlled 80% to 99% by 6 wk after treatment (WAT) following UAAS foliar herbicide treatments. The second case study demonstrated that UAAS directed in-water herbicide application reduced variable-leaf watermilfoil visible plant material by 94% at 5 WAT. Likewise, directed in-water applications from UAAS eliminated the need to deploy watercraft, which improved overall operational efficiency. Data from both case studies indicate that UAAS can provide an effective and efficient treatment strategy for floating-leaved and submersed plant control among common herbicide treatment scenarios. Future integration of UAAS in aquatic weed control programs is encouraged, especially among smaller treatment sites (≤4 ha) or where access limits traditional spray operations.

Allowing the use of two additional modes of action (MOAs), Enlist™ corn is a novelty in the continuum of herbicide-resistant crop development efforts that have occurred since the 1990s. Knowledge of Enlist corn tolerance to labeled herbicides and other herbicides within the same MOA for various use and/or exposure scenarios is not well established. Four site-year field experiments for preemergence (PRE) and postemergence (POST) applications were conducted at sites in Fayetteville (2021 and 2022) and Tillar (2020 and 2021), Arkansas, to evaluate Enlist corn response following PRE or POST applications of synthetic auxin herbicides or those that inhibit acetyl-CoA carboxylase (ACCase). A non-Enlist and an Enlist corn hybrid were used for each herbicide treatment to establish differential tolerance. Injury response to PRE application varied among site-years; clethodim was the only herbicide that occasionally caused significant (7% to 17%) injury to Enlist corn. None of the PRE treatments affected plant height, stand, or yield of Enlist corn; these responses were generally similar or better for Enlist corn compared to non-Enlist corn. Enlist corn showed significant injury to POST applications of florpyrauxifen-benzyl (>10%), fluazifop-P-butyl and quizalofop-P-ethyl (>5%), and clethodim and sethoxydim (>75%) 1 wk after application (WAA). These initial injury responses to clethodim and sethoxydim were generally reflected in Enlist corn yield; however, the minimal injury from fluazifop-P-butyl and quizalofop-P-ethyl did not affect yield. Injury to non-Enlist corn with POST-applied ACCase-inhibiting herbicides 2 WAA was >80%, resulting in a proportionate yield reduction. Even though florpyrauxifen-benzyl caused more initial injury to non-Enlist corn, yield reduction in non-Enlist corn was occasionally less than of Enlist corn, with both hybrids experiencing >75% yield reduction. In summary, Enlist corn may occasionally show transient injury even to labeled herbicides when applied POST, and even though the injury from florpyrauxifen-benzyl is initially mild, it nonetheless results in substantial yield loss.

The combination of florpyrauxifen-benzyl + 2,4-D is a new, pre-packaged herbicide mixture for use in pastures and hayfields in the United States. Unlike many other pasture herbicides, florpyrauxifen-benzyl + 2,4-D is reported to preserve white clover. However, limited research exists on the efficacy of florpyrauxifen-benzyl + 2,4-D on common weed species and on the level of tolerance of white clover to it. Field trials were conducted in Virginia in 2018 to 2020 to evaluate control of various broadleaf weeds with florpyrauxifen-benzyl + 2,4-D compared to other commonly used herbicides. Field and greenhouse studies were also carried out to assess white clover tolerance. Weed species evaluated included bulbous buttercup, Canada thistle, horsenettle, and broadleaf plantain. Florpyrauxifen-benzyl + 2,4-D provided 75% to 99% control of all weeds 90 d after application except for horsenettle (56%), while causing the least white clover injury of any herbicide treatment that was evaluated. Spring herbicide applications resulted in greater bulbous buttercup control compared to fall applications, but florpyrauxifen-benzyl + 2,4-D provided greater than 81% control from both application timings. There were no differences in aboveground biomass between white clover varieties; however, all herbicides reduced white clover biomass compared to a nontreated control. This research suggests that florpyrauxifen-benzyl + 2,4-D can improve overall forage quality by controlling broadleaf weeds in mixed grass-legume stands while preserving white clover.

Control of southern pine species that easily establish from seed, such as loblolly pine and slash pine (wilding pines), has historically been achieved economically through the use of prescribed fire or application of glyphosate or glyphosate and saflufenacil during site preparation. Currently, alternatives to glyphosate are being investigated for wilding pine control because of health and safety concerns over glyphosate reported by some organizations. Two exploratory studies in the Coastal Plain Region of Georgia investigated the potential of several herbicides for wilding pine control with 0.56 to 0.70 kg ha−1 of 0.9-kg ae imazapyr included in all herbicide treatments. Application timings for Study 1 were July and September (n = 8 treatments per timing), whereas Study 2 took place in July and early November (n = 4 treatments per timing). In Study 1, various rates of choline triclopyr, ester triclopyr, fluroxypyr, aminopyralid + florpyrauxifen-benzyl, and aminopyralid + triclopyr were tested, while two treatments contained glyphosate. Study 2 investigated mixtures containing flumioxazin, glufosinate, and triclopyr. Results for Study 1 revealed that the two treatments containing glyphosate had the greatest percent loblolly pine control after 120 d (87.5% and 88.6% control, respectively), while the next best control was offered by a treatment containing imazapyr plus 3.36 kg ha−1 choline triclopyr (52.6% control). July treatments offered better control than September treatments, but the efficacy of September treatments may have been impacted by a severe drought. In Study 2, treatments applied during early November that contained imazapyr and glufosinate or imazapyr, glufosinate, and flumioxazin resulted in 100% control of mixed loblolly and slash pine seedlings and saplings. All November treatments offered better control than July treatments in Study 2. Promising results from Study 2 suggest that glufosinate may warrant additional study for use in forestry site preparation as an alternative to glyphosate to control wilding pines.

Palmer amaranth is a common weed on levees in rice fields but has become increasingly problematic with the adoption of furrow-irrigated rice and lack of an established flood. Florpyrauxifen-benzyl previously has been found effective for controlling Palmer amaranth in rice, but the efficacy of low rates of florpyrauxifen-benzyl and the effect of Palmer amaranth size on controlling it is unknown. The objective of this research was to determine the level of Palmer amaranth control expected with single and sequential applications of florpyrauxifen-benzyl at varying weed heights. The first study was conducted near Marianna, AR, in 2019 and 2020, to determine the effect of florpyrauxifen-benzyl rate on control of <10 cm (labeled size) and 28- to 32-cm-tall (larger-than-labeled size) Palmer amaranth. The second experiment was conducted in 2020 at two locations in Arkansas to compare single applications of florpyrauxifen-benzyl at low rates to sequential applications at the same rates with a 14-d interval on 20- and 40-cm-tall Palmer amaranth. Results revealed that florpyrauxifen-benzyl at 15 g ae ha−1 was as effective as 30 g ae ha−1 in controlling <10-cm-tall Palmer amaranth (92% and 95% mortality in 2019). Sequential applications of florpyrauxifen-benzyl at 8 g ae ha−1 were as effective as single or sequential applications at 30 g ae ha−1. However, no rate of florpyrauxifen-benzyl applied to 20- or 40-cm-tall Palmer amaranth was sufficient to provide season-long control of the weed, with the escaping female plants producing as many as 6,120 seed per plant following a single application.

Environmental conditions surrounding herbicide applications are known to affect weed control and crop response. Variable levels of rice injury caused by florpyrauxifen-benzyl have been observed across cropping systems and environmental conditions, warranting research in which single environmental and management strategies are isolated to understand the effect of each factor on rice injury and subsequent reductions in rice growth. A field study was conducted to determine the effects of planting date, rice cultivar, and florpyrauxifen-benzyl rate on rice injury, maturity, and yield. Two greenhouse studies were conducted to determine the effect of soil moisture and time of flooding after florpyrauxifen-benzyl application on rice injury caused by the herbicide. Growth chamber experiments were conducted to isolate the effects of temperature and light intensity on rice injury caused by florpyrauxifen-benzyl. In the field study, levels of injury varied across planting dates in both years, indicating the influence of environment on the crop response to florpyrauxifen-benzyl applications. Under dry (40% soil moisture) and saturated (100%) soil conditions, rice injury increased to 36% and 35%, respectively, compared with 27% and 25% injury at 60% and 80% soil moisture, respectively. Flooding rice 0 to 6 d after florpyrauxifen-benzyl application reduced visible injury; however, a reduction in rice tiller production occurred when the rice was flooded the same day as application. Visible rice injury increased when florpyrauxifen-benzyl was applied under low light intensity (700 µmol m−2 s−1) and high temperatures (35/24 C day/night). Based on these findings, applications of florpyrauxifen-benzyl are least likely to cause unacceptable rice injury when applied to soils having 60% and 80% saturation in high light, low temperature environments, and the crop is flooded 3 to 6 d following application.

Smallflower umbrella sedge is one of the most problematic weeds in direct-seeded rice in India. Bispyribac-sodium (acetolactate synthase [ALS]-inhibiting herbicide) is commonly used in rice, but growers have recently reported lack of smallflower umbrella sedge control with this herbicide. An extensive survey was carried out in two rice-growing states, Chhattisgarh and Kerala, where 53 putative bispyribac-sodium-resistant (BR) biotypes were collected. Studies were conducted to confirm resistance to bispyribac-sodium and to test the efficacy of the newly developed synthetic auxin herbicide florpyrauxifen-benzyl on putative BR biotypes. A whole-plant bioassay revealed that bispyribac-sodium is no longer effective. Of 53 putative BR biotypes, 17 biotypes survived the recommended label rate of 25 g ai ha−1. The effective bispyribac-sodium rate required to control 50% of the plants in most of the BR biotypes (ED50) ranged from 19 to 96 g ha−1, whereas it was 10 g ha−1 in a susceptible biotype. In two highly resistant biotypes, the ED50 was beyond the maximum tested rate, 200 g ha−1. This suggests 2- to >20-fold resistance in BR biotypes. An ALS enzyme activity assay suggests an altered target site as mechanism of resistance to bispyribac-sodium. This study confirms the first case of evolved resistance to bispyribac-sodium in smallflower umbrella sedge in India. However, the newly developed synthetic auxin florpyrauxifen-benzyl effectively controlled all BR biotypes at the field use rate of 31.25 g ai ha−1.

Many factors such as environment, herbicide rate, growth stage at application, and days between sequential applications can influence the response of a crop to herbicides. Florpyrauxifen-benzyl is a new broad-spectrum, POST herbicide that was commercialized for use in U.S. rice production in 2018. Field experiments were conducted in 2018 at the Pine Tree Research Station (PTRS) near Colt, AR, and the Rice Research and Extension Center (RREC), near Stuttgart, AR, to evaluate crop injury and yield response of three rice cultivars to sequential applications of florpyrauxifen-benzyl. Greenhouse and growth chamber experiments were conducted at the Altheimer Laboratory in Fayetteville, AR, to evaluate cultivar responses when florpyrauxifen-benzyl was applied at 30 or 60 g ae ha−1 to rice exposed to different temperature regimes or at various growth stages. Three rice cultivars were used in all experiments: long-grain variety ‘CL111’, medium-grain variety ‘CL272’, and long-grain hybrid cultivar ‘CLXL745’. CL111 exhibited sufficient tolerance to florpyrauxifen-benzyl with only 10% visible injury and no effect on yield. CL272 showed 15% injury 3 wk after the second application in the field experiment when applications were made 14 d apart. Additionally, 12% injury was observed in greenhouse studies when florpyrauxifen-benzyl was applied at 30 g ae ha−1, averaged over various growth stages at application. Florpyrauxifen-benzyl did not reduce the yield of CL272 in field experiments, indicating that CL272 can recover from florpyrauxifen-benzyl injury. As much as 64% injury was observed for CLXL745 at 3 wk after application (WAA) when sequential herbicide applications were made 4 d apart. High levels of injury occurred in the growth chamber and greenhouse studies for this cultivar as well. Sequential applications of florpyrauxifen-benzyl reduced yields of CLXL745 in nearly all treatments. Data from these experiments suggest that CL272 and CLXL745 are sensitive to sequential applications of florpyrauxifen-benzyl. Growers must follow the prescribed guidelines for using florpyrauxifen-benzyl in these cultivars and others like it.

Florpyrauxifen-benzyl and quizalofop were available for POST applications in 2018; however, little is known about the response of acetyl-CoA carboxylase (ACCase)–resistant rice cultivars and advanced lines to POST herbicides. A field study was conducted in 2017 and 2018 at Stoneville, MS, to characterize the response of ACCase-resistant rice cultivars and advanced lines to POST applications of florpyrauxifen-benzyl. The imidazolinone-resistant (IR) rice cultivars ‘CL163’ and ‘CLXL 745’, and ACCase-resistant rice cultivars ‘PVL01’, ‘PVL013’, ‘PVL024-B’, ‘PVL038’, ‘PVL080’, and ‘PVL081’were treated with florpyrauxifen-benzyl at 0 (nontreated control for each cultivar) and 58 g ai ha–1 at the four-leaf to one-tiller (LPOST) growth stage. At 14 d after treatment (DAT), PVL01 was injured 5% to 6% greater than CLXL 745, PVL013, and PVL081; however, injury was ≤10% at that evaluation for all cultivars. Similarly, injury was ≤13% for all cultivars 28 DAT. Mature heights were reduced for all cultivars except PVL013 and PVL081. Rough rice yield was ≥100% of the control for all cultivars except PVL081, PVL013, and CL163. Results suggest that florpyrauxifen-benzyl can safely be applied POST to rice cultivars grown in Mississippi as well as ACCase-resistant cultivars that are currently under development.

A glasshouse study was conducted on the Louisiana State University campus in Baton Rouge, LA, to evaluate the control of brook crowngrass, rice cutgrass, southern watergrass, and water paspalum. Florpyrauxifen-benzyl was applied at 30 g ai ha−1 to each grass species at the 3- to 4-leaf or 1- to 2-stolon stage of growth. Brook crowngrass treated with florpyrauxifen was controlled 71% at 21 d after treatment. Southern watergrass and water paspalum control did not exceed 56% and 36%, respectively, across all evaluations. Rice cutgrass treated with florpyrauxifen did not reach 15% control. Plants treated with florpyrauxifen, except rice cutgrass, displayed reduction in leaf number, stolon number, plant height, and plant fresh weight. These results indicate florpyrauxifen-benzyl can help manage a brook crowngrass infestation and suppress southern watergrass. However, florpyrauxifen-benzyl has little to no activity on water paspalum and rice cutgrass, and other management options should be employed if these weeds are present.

Florpyrauxifen-benzyl is a new synthetic auxin herbicide that will provide a novel site of action in rice production. In many areas of the United States it is common practice to plant soybeans in rotation with rice, thereby introducing the potential for herbicide carryover. Multiple field experiments were conducted in 2014 and repeated in 2015 to evaluate potential plant-back restrictions for soybean and other row crops following an application of florpyrauxifen-benzyl. In the first experiment, treatments comprised florpyrauxifen-benzyl applied at 40 followed by 40 g ai ha–1, 80 fb 80 g ai ha–1, and a nontreated check. In 2014, herbicides were applied to a silt loam soil near Stuttgart and Colt, AR, and fields remained fallow following application. The following year, corn, cotton, soybean, grain sorghum, and sunflower were planted within the previously treated area. Stand counts, crop heights, and visual injury assessments were done for each crop following planting, and aboveground biomass data were collected 28 d after planting. No significant differences were observed among the treatments for any of the parameters assessed, highlighting the rotational flexibility of common row crops 1 yr following a florpyrauxifen-benzyl application. In the second experiment, florpyrauxifen-benzyl was applied at 30 and 60 g ai ha–1 at 56, 28, 14, and 0 d before planting soybean. Injury assessments corresponded to the highest concentration of florpyrauxifen-benzyl and its metabolites recovered from soil at the time of planting. Conversely, soybean injury was reduced when florpyrauxifen-benzyl was applied at increasing intervals before planting. At the end of each season, soybean yield was similar to the nontreated control when florpyrauxifen-benzyl at 30 or 60 g ai ha–1 was applied 56 d before planting, whereas all other treatments reduced yield. These results support a relatively short replant interval for soybean after florpyrauxifen-benzyl application to rice.

Florpyrauxifen-benzyl is a new active ingredient that represents an additional tool in rice (Oryza sativa L.) weed control by providing an alternative mechanism of action. Studies were conducted to evaluate soil moisture influences on florpyrauxifen-benzyl absorption, translocation, and metabolism in three problematic weeds. In the absorption/translocation study, barnyardgrass [Echinochloa crus-galli (L.) P. Beauv.], hemp sesbania [Sesbania herbacea (Mill.) McVaugh], and yellow nutsedge (Cyperus esculentus L.) were treated with [14C]florpyrauxifen-benzyl under two soil moisture regimes (7.5% and 60% field capacity). Greater absorption occurred under moist conditions (60% soil moisture content). More translocation of the herbicide to the area above the treated leaf occurred under moist versus dry soil across all weed species. Sesbania herbacea translocated 25% of the absorbed herbicide above the treated leaf, a result greater than that of the other two weed species at 60% soil moisture. However, no differences in translocation occurred among the weed species at the 7.5% soil moisture regime. In the metabolism study, 95% of the herbicide recovered was in its acid form under the high soil moisture regime for S. herbacea, a species that shows extreme sensitivity to even low doses of this herbicide, and soil moisture influenced the amount of acid form found in all species. While these data provide a limited view into the physiological processes being affected, they do suggest that for E. crus-galli, S. herbacea, and C. esculentus, soil moisture content in the field will likely play a significant role in absorption, translocation, and metabolism of florpyrauxifen-benzyl.

In a greenhouse experiment, soybean, cotton, corn, grain sorghum, and sunflower were subjected to 1/10 (3 g ai ha-1), 1/100 (0.3 g ai ha-1), or 1/500 (0.06 g ai ha-1) of the 1X rate of florpyrauxifen-benzyl. Visible injury 14 days after treatment (DAT) was the greatest with soybean (96%) when exposed to the highest drift rate of 1/10x or 3 g ai/ha-1 of florpyrauxifen-benzyl and was significantly higher than all other crops and drift rates. Cotton and sunflower were also injured 85 and 83%, respectively, by the 1/10x rate but had less injury when a 1/100x or 1/500x rate was applied (injury ranging from 9 to 33%). It was concluded that the negative effects on soybean, cotton, and sunflower primarily resulted from exposure to the highest rate tested (1/10x) and only soybean expressed negative effects even at the lower rate of 1/100x. A field study was also conducted to (1) evaluate the sensitivity of soybean to low concentrations of florpyrauxifen-benzyl during vegetative and reproductive development and (2) compare soybean injury and yield following applications of florpyrauxifen-benzyl and dicamba across various growth stages and concentrations. Soybean plants were treated with 1/10, 1/20, 1/40, 1/80, 1/160, 1/320, or 1/640 of the 1X rate of florpyrauxifen-benzyl (30 g ai/ ha-1) or dicamba (560 g ae ha-1) at the V3 and R1 growth stage. Florpyrauxifen-benzyl applied at a rate of 1/10 to 1/40X caused foliar injury and subsequent height reduction. In comparison, dicamba applied at the same rates caused slightly less injury and growth reductions. As rate of florpyrauxifen-benzyl decreased from 1/10 to 1/640X, the level of soybean injury dissipated rather quickly. However, this was not the case with dicamba, as substantial injury was observed with rates as low as 1/640X.