Goosegrass and smooth crabgrass in creeping bentgrass turf are difficult to control due to a lack of selective herbicides. Based on preliminary field observations, we hypothesized that paclobutrazol and flurprimidol would reduce the overall competitiveness of goosegrass and smooth crabgrass in creeping bentgrass. Greenhouse and field studies were designed to evaluate the effect of several plant growth regulators (PGRs) on goosegrass and smooth crabgrass competitive indices. In greenhouse studies, flurprimidol, paclobutrazol, trinexapac-ethyl, and prohexadione-calcium were applied either preemergence only or preemergence plus two biweekly postemergence applications to goosegrass and smooth crabgrass plants to simulate the first 1.5 mo of typical PGR programs used on golf courses. Two weeks after the final postemergence treatment, aboveground biomass and root biomass were recorded. Programmatic flurprimidol and paclobutrazol applications reduced smooth crabgrass aboveground biomass by 67% and 69%, respectively, and more than trinexapac ethyl or prohexadione-calcium. When averaged across application programs, flurprimidol and paclobutrazol reduced smooth crabgrass root biomass by 74% and goosegrass biomass by 73% to 80%. Field studies were established to further evaluate the influence of PGRs on smooth crabgrass coverage in creeping bentgrass turf. Treatments consisting of flurprimidol, trinexapac-ethyl, flurprimidol plus trinexapac-ethyl, paclobutrazol, and fenoxaprop-p were applied every 3 wk from April to August. Weed coverage data were collected throughout the growing season, and final smooth crabgrass control data were collected at the end of the season. In general, flurprimidol-containing treatments more effectively reduced smooth crabgrass coverage throughout the growing season than trinexapac ethyl. After the studies, regimens that contained flurprimidol controlled smooth crabgrass by 68% to 73%, greater than any other PGR program evaluated. Results from these studies indicate that flurprimidol may be used to effectively control smooth crabgrass or goosegrass in creeping bentgrass turf. These are the first reported data regarding the use of flurprimidol for smooth crabgrass or goosegrass control in turf.

Methiozolin is labeled for goosegrass and smooth crabgrass control in golf course putting greens, but no peer-reviewed literature exists regarding this use. Greenhouse experiments were conducted evaluating goosegrass and smooth crabgrass response to increasing rates of methiozolin as affected by weed growth stage. In general, as weed growth stage increased, the methiozolin rate required to reduce weed biomass 90% (WR90) increased. Goosegrass was more sensitive to preemergence-applied methiozolin than smooth crabgrass, and the WR90 was 30.4 and 118 g ai ha–1 for goosegrass and smooth crabgrass, respectively. However, smooth crabgrass was generally more sensitive to postemergence-applied methiozolin than goosegrass. Subsequent field studies were conducted to evaluate goosegrass and smooth crabgrass control with methiozolin applied singularly or sequentially at standard preemergence timings. Results indicated methiozolin applied singularly or sequentially at the label-recommended rate (500 g ha–1) is not persistent enough to provide season-long control of goosegrass and smooth crabgrass. Ten field studies were conducted in Alabama, California, Florida, and Virginia to evaluate frequent methiozolin application programs with the objective of providing selective, season-long goosegrass and smooth crabgrass control. Results from these studies indicate methiozolin can be safely applied to hybrid bermudagrass and creeping bentgrass putting greens despite exceeding the yearly maximum use rate for putting greens (2,500 g ha–1) with some treatments. Methiozolin effectively controlled smooth crabgrass throughout the growing season in California and Virginia when 10 biweekly applications were applied at 250 g ha–1 or higher. In Florida, methiozolin did not acceptably (80%) control goosegrass regardless of application rate. In Virginia, methiozolin acceptably controlled goosegrass only when applied at rates and frequencies that exceeded the maximum yearly methiozolin usage rate. These data indicate that methiozolin has the potential to control smooth crabgrass preemergence when applied frequently, but does not provide acceptable goosegrass control at labeled rates.

We conducted research to evaluate various herbicides for POST false-green kyllinga control in cool-season turfgrass (primarily creeping bentgrass). In a preliminary evaluation, single and sequential applications of halosulfuron-methyl (70 g ai ha−1), mesotrione (175 g ai ha−1), and sulfentrazone (140 g ai ha−1), as well as a single application of imazosulfuron (740 g ai ha−1), were evaluated in New Jersey. Imazosulfuron and sequential applications of halosulfuron-methyl controlled false-green kyllinga >93% at 9 and 18 wk after initial treatment (WAIT). Sulfentrazone and mesotrione controlled false-green kyllinga <50%. Additional experiments were conducted to evaluate single and sequential applications of halosulfuron-methyl (70 g ha−1), imazosulfuron (420 and 740 g ha−1), and sulfentrazone (140 g ha−1) in New Jersey and Indiana at two locations in each state. At 12 WAIT, imazosulfuron generally controlled false-green kyllinga more effectively than other treatments at all locations. Sequential applications of imazosulfuron controlled false-green kyllinga 100% at 12 WAIT. Halosulfuron-methyl was less effective in Indiana than in New Jersey. Sulfentrazone controlled false-green kyllinga <40% at 12 WAIT. This research demonstrates that imazosulfuron is more effective than halosulfuron-methyl and sulfentrazone for POST false-green kyllinga control in cool-season turf.

Methiozolin is an isoxazoline herbicide that selectively controls annual bluegrass in cool-season turf and may control roughstalk bluegrass, another weedy Poa species that is problematic in many turfgrass systems. However, the majority of research to date is limited to evaluating methiozolin efficacy for annual bluegrass control in creeping bentgrass putting greens. Research was conducted comparing various application regimes of methiozolin and other herbicides for long-term roughstalk bluegrass control in creeping bentgrass golf fairways. Methiozolin-only treatments did not injure creeping bentgrass or reduce normalized difference vegetative index (NDVI) at 2 golf course locations based on 20 evaluation dates over a 2.5-yr period. The 2.5-yr average turf quality generally declined as roughstalk bluegrass control increased due to transient turf cover loss. At 1 yr after last treatment, methiozolin at 1500 g ai ha-1 applied four times in fall reduced roughstalk bluegrass cover 85%. This was equivalent to methiozolin at 1000 g ha-1 applied four times in fall, but greater than low rates of methiozolin applied four times in spring or twice in fall and spring. Amicarbazone, primisulfuron, and bispyribac-sodium alone either did not effectively reduce roughstalk bluegrass cover, or did so at the expense of increased creeping bentgrass injury. Results of this study suggest that methiozolin alone or tank-mixed with amicarbazone or primisulfuron is an effective long-term approach for selectively controlling roughstalk bluegrass in creeping bentgrass.

Experiments were conducted to compare rates and formulations of quinclorac for postemergent control of slender speedwell in cool-season turf. A 50% ae wettable powder formulation (50WP) was compared with two granular formulations, a 1% clay granule (1G) and a 0.4% corn cob granule (0.4G). Each formulation was applied at 0.3, 0.6, 0.8, and 1.1 kg ha−1. Additionally, 2,4-D at 1.1 kg ha−1 was applied alone and in combination with quinclorac 50WP at 0.8 kg ha−1. Slender speedwell control with the 50WP or 0.4G formulations was superior to that obtained with the 1G. At lower rates, slender speedwell control with the 50WP was greater than with the 0.4G; however, the rates required to obtain 95% control were not significantly different. Tank mixing quinclorac 50WP at 0.8 kg ha−1 with 2,4-D at 1.1 kg ha−1 did not improve slender speedwell control but increased roughstalk bluegrass injury. Kentucky bluegrass, perennial ryegrass, and tall fescue were not injured by quinclorac. Roughstalk bluegrass and chewings fescue were temporarily discolored. Turf injury was rate-related and more severe with the 50WP than the 1G formulation.

Two field studies, a seeded study and a golf course study, were conducted to compare competition among creeping bentgrass, annual bluegrass, and roughstalk bluegrass when subjected to common weed control practices and foliar applications of iron and magnesium. A research site was selected for the seeded study and divided into 10 whole plots receiving irrigation at either 50 or 100% evapotranspiration deficit. Each whole plot was further divided into subplots receiving one of seven treatments: bensulide, ethofumesate, trinexapac-ethyl, foliar Mg, foliar Fe, foliar Mg plus foliar Fe, and control. The site was seeded to a mixture of creeping bentgrass, annual bluegrass, and roughstalk bluegrass in September 1995, and treatments began in March 1996. Annual bluegrass was reduced 29% in plots treated with foliar Fe and 65% in plots treated with foliar Fe plus foliar Mg. Roughstalk bluegrass was significantly reduced in seeded plots treated with foliar iron (50%), plant growth regulator (75%), and foliar iron plus foliar magnesium (100%). Annual bluegrass and roughstalk bluegrass proportions were not affected by irrigation regime. In a second study, the most effective treatment, foliar magnesium plus foliar iron, was tested on a working golf course fairway and on a practice putting green beginning April 1997 and ending November 1997. Treatments on the golf course fairway and practice putting green were ineffective due to the established, perennial nature of the annual bluegrass biotypes on these sites. Further research is required to improve the efficacy of nutritional treatments on these perennials.

Methiozolin is a new isoxazoline herbicide that has scarcely been tested in Kentucky bluegrass turf. A field trial was conducted in Blacksburg, VA, to determine response of 110 Kentucky bluegrass varieties and winter annual weeds to sequential fall applications of methiozolin. At 1.5 and 6 mo after initial treatment (MAIT), Kentucky bluegrass injury I30 values (predicted methiozolin rate that causes 30% Kentucky bluegrass injury) ranged between 3.4 to more than 10 times the recommended methiozolin rate for annual bluegrass control. Methiozolin at all rates reduced cover of annual bluegrass, common chickweed, corn speedwell, hairy bittercress, mouseear chickweed, and Persian speedwell but increased cover of parsley-piert. For all varieties, methiozolin at 2 kg ai ha−1 increased Kentucky bluegrass cover, turf quality, and turf normalized difference vegetation index (NDVI) relative to the nontreated check at 6 MAIT. Kentucky bluegrass relative cover change (RCC) was attributed primarily to weed control but was inversely correlated with methiozolin rates because of increased weed control and reduced Kentucky bluegrass growth. Despite the decline in RCC with increasing methiozolin rates, most Kentucky bluegrass varieties treated with the highest methiozolin rate (6 kg ha−1) still had greater Kentucky bluegrass cover than the nontreated check at 6 MAIT. Results from this study indicate that two fall applications of methiozolin at rates beyond that previously reported for annual bluegrass control can safely be applied to a broad range of Kentucky bluegrass varieties spanning most of the known genetic classifications.

Methiozolin selectively controls annual bluegrass in cool-season turfgrasses, and practitioners may wish to reseed desirable species in treated areas. Field experiments were conducted to evaluate reseeding intervals for creeping bentgrass, perennial ryegrass, and tall fescue following methiozolin applications. Turfgrass establishment varied for species, application timing (0, 2, 4, or 6 wk before seeding, WBS), and rates tested (0.56, 1.12, or 2.24 kg ai ha−1). Reductions in turf cover suggest that seeding of creeping bentgrass, perennial ryegrass, and tall fescue should be delayed 2 wk after methiozolin treatments at 0.56 kg ha−1. However, reseeding should be delayed after methiozolin treatments at 1.12 kg ha−1 for approximately 4, 4, and 2 wk for creeping bentgrass, perennial ryegrass, and tall fescue, respectively. Similarly, establishment was reduced on all dates from the nontreated after 2.24 kg ha−1 was applied at 4 WBS, suggesting that reseeding should be delayed for at least 6 wk on all three species at the high rate.

Methiozolin is a new isoxazoline herbicide being investigated for selective POST annual bluegrass control in creeping bentgrass putting greens. Glasshouse and field research was conducted from 2010 to 2012 in Tennessee and Texas to evaluate annual bluegrass control efficacy with methiozolin. Application placement experiments in the glasshouse illustrated that root absorption was required for POST annual bluegrass control with methiozolin at 1,000 g ai ha−1. Soil-plus-foliar and soil-only applications of methiozolin reduced annual bluegrass biomass greater than treatments applied foliar-only. Field experiments evaluated annual bluegrass control efficacy with two application rates (500 and 1,000 g ha−1) and six application regimes (October, November, December, October followed by [fb] November, November fb December, and October fb November fb December) on sand- and soil-based putting greens. Annual bluegrass control with methiozolin at 1,000 g ha−1 on sand-based greens ranged from 70 to 72% compared to 87 to 89% on soil-based greens. Treatment at 500 g ha−1 controlled annual bluegrass 57 to 64% on sand-based greens compared to 72 to 80% on soil-based greens. Most sequential methiozolin application regimes controlled annual bluegrass more than single applications. On sand-based greens, sequential application programs controlled annual bluegrass 70 to 79% compared to 85 to 92% on soil-based greens. Responses indicate that methiozolin is a root-absorbed herbicide with efficacy for selective control of annual bluegrass in both sand- and soil-based creeping bentgrass putting greens.

Broadleaf weeds are common and troublesome pests in cool-season turfgrass species such as tall fescue, Kentucky bluegrass, perennial ryegrass, and creeping bentgrass. Broadleaf weeds are primarily managed in these grasses through POST applications of growth regulator herbicides in the phenoxy, benzoic acid, and pyridine chemical classes. There are disadvantages to use of these chemicals, including nontarget plant damage and limited residual control. Certain annual broadleaf weeds can be controlled through application of isoxaben or a PRE crabgrass herbicide, but these herbicides do not control emerged broadleaf weeds. There are advantages to use of sulfonylurea herbicides, including PRE and POST control of annual and perennial weeds, a different mode of action, and these herbicides have low vapor pressure, reducing the potential for offsite movement. There are disadvantages to the use of sulfonylurea herbicides, including limited spectrum of broadleaf weed species controlled and limited tolerance in cool-season turfgrass species. The primary sulfonylurea herbicides used in cool-season turfgrass are chlorsulfuron, halosulfuron, metsulfuron, and sulfosulfuron. There have been specialized uses for primisulfuron and tribenuron-methyl.

Methiozolin and cumyluron are experimental herbicides that are reported to control annual bluegrass PRE or POST; however, no studies have compared these new herbicides to currently-registered herbicides for annual bluegrass control on putting greens over multiple years. Studies were conducted on three Virginia putting greens for 2 yr to compare methiozolin and cumyluron each at two rates to bensulide and bensulide plus oxadiazon at labeled rates for effects on annual bluegrass and creeping bentgrass cover, turf injury, normalized difference vegetative index (NDVI), turf quality, and annual bluegrass seedhead suppression. Methiozolin, cumyluron, bensulide, and bensulide plus oxadiazon did not significantly injure creeping bentgrass putting green turf, reduce quality, or reduce NDVI. Only methiozolin at 500 or 750 g ai ha−1 and cumyluron at 8,600 g ai ha−1 reduced area under the progress curve (AUPC) for annual bluegrass cover following four treatments over 2 yr applied in spring and fall each year. A concomitant increase in creeping bentgrass cover AUPC was also observed from the three treatments that reduced annual bluegrass cover. Methiozolin also reduced annual bluegrass seedhead cover at least 85% 1 mo after spring treatments and more than all other treatments except cumyluron at 8,600 g ha−1 (66%). These studies suggest that single treatments of methiozolin in spring and fall will not rapidly control existing annual bluegrass but can slowly reduce populations over time, presumably by preventing new seedling emergence. Methiozolin and cumyluron appear to be more effective than currently available herbicides bensulide and bensulide plus oxadiazon for PRE annual bluegrass control and seedhead suppression on golf putting greens.

Recent advances in genetic engineering have led to the development of glyphosate-resistant (GR) crops for genetic markers and selective weed control. The effects of glyphosate residue on turfgrass clippings could be toxic to non-GR species. The objective of this experiment was to determine whether glyphosate would retain activity within clippings of creeping bentgrass when applied to Kentucky bluegrass and perennial ryegrass. Greenhouse-grown ‘Penncross’ and GR ‘ASR-368’ were treated with glyphosate at 2.24 kg/ha. Clippings were collected 1, 3, 7, and 12 d after application and applied to greenhouse-grown Kentucky bluegrass and perennial ryegrass. Kentucky bluegrass and perennial ryegrass dry weight and percent cover were reduced by clippings receiving glyphosate that were harvested 1 and 3 d after glyphosate application from both susceptible and resistant creeping bentgrass. Results indicate that glyphosate remains active in clippings for up to 3 d after treatment within creeping bentgrass clippings. Glyphosate-applied creeping bentgrass clippings will need to be managed to prevent injury to susceptible species.

Amicarbazone controls annual bluegrass in cool-season turfgrasses but physiological effects that influence selectivity have received limited investigation. The objective of this research was to evaluate uptake, translocation, and metabolism of amicarbazone in these species. Annual bluegrass, creeping bentgrass, and tall fescue required < 3, 56, and 35 h to reach 50% foliar absorption, respectively. At 72 h after treatment (HAT), annual bluegrass and creeping bentgrass translocated 73 and 70% of root-absorbed 14C to shoots, respectively, while tall fescue only distributed 55%. Annual bluegrass recovered ≈ 50% more root-absorbed 14C in shoots than creeping bentgrass and tall fescue. Creeping bentgrass and tall fescue metabolism of amicarbazone was ≈ 2-fold greater than annual bluegrass from 1 to 7 d after treatment (DAT). Results suggest greater absorption, more distribution, and less metabolism of amicarbazone in annual bluegrass, compared to creeping bentgrass and tall fescue, could be attributed to selectivity of POST applications.

Glasshouse research was conducted to investigate the efficacy of herbicide safeners for improving creeping bentgrass (CBG) tolerance to various herbicides. CBG injury from amicarbazone (150 g ha−1), bispyribac-sodium (110 g ha−1), fenoxaprop-p-ethyl (35 g ha−1), imazapic (45 g ha−1), quinclorac (1,050 g ha−1), or topramezone (37 g ha−1) applied in combination with the herbicide safeners naphthalic anhydride or isoxadifen-ethyl was evaluated. These safeners reduced CBG injury from topramezone only. Topramezone was then applied in combination with naphthalic anhydride, isoxadifen-ethyl, cloquintocet-mexyl (cloquintocet), fenchlorazole-ethyl, mefenpyr-diethyl, and benoxacor. These experiments determined that CBG injury was lowest from topramezone in combination with cloquintocet. Additional experiments evaluated topramezone (37 g ha−1) with several rates of cloquintocet and determined that applications at ≥ 28 g ha−1 reduced CBG injury similarly. Cloquintocet (28 g ha−1) increased topramezone I50 values against CBG, but not large crabgrass or goosegrass. The cytochrome P450 (cP450) inhibitor malathion (1000 g ha−1) reduced topramezone I50 values against CBG in one experimental run. Topramezone–cloquintocet combinations warrant further research in field settings.

Methiozolin is a new herbicide from South Korea currently under development in the United States for PRE and POST annual bluegrass control in bentgrass and most other cool- and warm-season turfgrasses. Greenhouse studies were conducted in 2012 at the University of California, Riverside, CA, and Auburn University, Auburn, AL, to evaluate the relative tolerance of three bentgrass species comprised of nine creeping bentgrass (CRBG) cultivars, velvet bentgrass (VBG) and colonial bentgrass (COBG) to methiozolin at 0, 0.6, 1.1, 2.2, 4.5 and 9.0 kg ai ha−1. Methiozolin was applied 7 wk after seeding, followed by a second application 5 wk later. Methiozolin rates that produced 25% injury (TI25) and 50% clipping dry weight reduction (GR50) relative to a nontreated control for each species or cultivar were calculated using four-parameter logistic regression. Turf injury rates at 21 d after second treatment (DAT2) were the most consistent in describing relative tolerance among bentgrass species. Overall, CRBG was more tolerant to methiozolin than VBG or COBG. After two applications, methiozolin rates that caused TI25 were 1.1, 0.2, and 0.3 kg ha−1 for CRBG (across all cultivars), VBG, and COBG, respectively. VBG and COBG were not tolerant of sequential methiozolin applications at rates necessary to control annual bluegrass under field conditions. Herbicide rates that caused TI25 and GR50 decreased with the second application. ‘Penn A-4’ CRBG exhibited the highest TI25 28 d after initial treatment (DAIT) at University of California at Riverside (4.5 kg ha−1), but only 2.5 kg ha−1 with two applications by 21 DAT2. All CRBG cultivars tested tolerated methiozolin at 0.5 kg ha−1, the recommended sequential use rate for putting greens in Korea.