Ascorbate modulates IK(V) of ON-type
mixed rod/cone bipolar cells (Mb) in the goldfish retinal slice
through a dopamine D1/G-protein/PKA-coupled mechanism. We
investigated the effects of dopamine depletion with intraocular
injections of 6-OHDA on IK(V) and its modulation
by ascorbate over 1–7 weeks following 6-OHDA treatment. Dopamine
depletion was verified by tyrosine hydroxylase immunocytochemistry.
Slices were perfused in a saline containing 200 μM sodium ascorbate.
One-second puffs of ascorbate-free saline (zero [AA]o),
delivered through a 2–3 μm diameter pipette, were directed at
the bipolar cells. IK(V) was recorded by conventional
whole-cell patch-clamp methods. In normal retinas, puffs of zero
[AA]o caused a rapid (<100 ms) suppression of
IK(V) of about 50% that lasted for several minutes.
This effect was blocked by 1 μM SCH23390 and was unaffected by 2 mM
Co2+ or 5 μM spiperone. 6-OHDA treatment resulted in major
effects. First, IK(V) was reduced by ∼50% for
weeks 1–6, recovering to a 20% reduction by week 7. Second, puffs of
zero [AA]o enhanced IK(V) rather
than suppressed it. The enhancement was blocked by SCH23390 and the PKA
inhibitor, Wiptide, but was insensitive to spiperone. Third, all parts of
the Mb bipolar cell (except for the axon) were sensitive to puffs of zero
[AA]o in both normal and 6-OHDA-treated retinas.
Fourth, bath application of 20 μM dopamine restored the amplitude
of IK(V) but did not reverse the effects of puffed
zero [AA]o. IK(V) was fit by two
exponentials; all of the effects on IK(V) were on the
amplitude of the components and not on the time constants. Chronic dopamine
depletion caused reversible changes in the properties of K+
channels underlying IK(V), as well as a long-term change
in the intracellular coupling mechanisms between D1-receptor activation and the modulation of IK(V).