Based on morphological and genetic characteristics, we describe a new species of Hepatozoon in the European wild cat (Felis silvestris silvestris), herein named Hepatozoon silvestris sp. nov. The study also provides the first data on the occurrence of H. felis in this wild felid. Hepatozoon meronts were observed in multiple cross-sections of different organs of four (44%) cats. Additionally, extracellular forms, resembling mature gamonts of Hepatozoon, were found in the spleen and myocardium of two cats. Furthermore, tissues of six animals (67%) were positive by PCR. Hepatozoon felis was identified infecting one cat (11%), whereas the 18S rRNA sequences of the remaining five cats (56%) were identical, but distinct from the sequences of H. felis. Phylogenetic analyses revealed that those sequences form a highly supported clade distant from other Hepatozoon spp. Future studies should include domestic cats from the areas where the wild cats positive for H. silvestris sp. nov. were found, in order to investigate their potential role to serve as intermediate hosts of this newly described species. Identification of its definitive host(s) and experimental transmission studies are required for elucidating the full life cycle of this parasite and the possible alternative routes of its transmission.

Population monitoring is important for conservation management but difficult to achieve for rare, cryptic species. Reliable information about the Critically Endangered Scottish wildcat Felis silvestris silvestris is lacking because of difficulties in morphological and genetic identification, resulting from extensive hybridization with feral domestic cats Felis catus. We carried out camera-trap surveys in the Cairngorms National Park, UK, to examine the feasibility of camera trapping, combined with a pelage identification method, to monitor Scottish wildcats. Camera trapping detected individually identifiable wildcats. Of 13 individual wild-living cats, four scored as wildcats based on pelage characters and the rest were wildcat × domestic cat hybrids. Spatially explicit capture–recapture density estimation methods generated a density of wild-living cats (wildcats and hybrids) of 68.17 ± SE 9.47 per 100 km2. The impact of reducing trapping-grid size, camera-trap numbers and survey length on density estimates was investigated using spatially explicit capture–recapture models. Our findings indicate camera trapping is more effective for monitoring wildcats than other methods currently used and capture success could be increased by using bait, placing camera stations ⩽ 1.5 km apart, increasing the number of camera stations, and surveying for 60–70 days. This study shows that camera trapping is effective for confirming the presence of the wildcat in potential target areas for conservation management.