Fungal spores, especially those of coprophilous fungi, are present in dung middens of Rhinoceros unicornis (greater one-horned rhinoceros) in both forest and grassland areas of the Kaziranga National Park, India. The presence of coprophilous fungi on rhino dung, chiefly Sporormiella, Saccobolus, Ascodesmis, Cercophora, and Sordaria, is documented for the first time. The Sporormiella–Ascodesmis–Saccobolus assemblage is abundant and characterizes the rhino dung in forest and grassland areas. The presence of coprophilous fungi spores allows for an examination of the relationship between rhinoceros ecology and the flora and other fauna in the region. The overall dataset is useful in interpreting the present and past distribution of rhino and other associated animals based on the relative abundance of different types of coprophilous fungi spores and their relationship to paleoherbivory and paleoecology in India and adjoining areas.