We rely on prospective memory (PM) to carry out intended actions at an appropriate time in the future. This ability is very important for day-to-day functioning, for example, remembering to take medication, attend appointments and pay bills. The literature covering the topic of PM has increased exponentially in the past 20–30 years. This paper will describe this relatively novel form of memory and discuss the debilitating effect PM deficits have on everyday living. It will also examine the populations who experience significantly more frequent and severe PM impairments and explore the causes and mechanisms of PM impairments by reviewing behavioural, clinical, genetic and neuroimaging studies. The paper will conclude by discussing the latest research into ways of treating PM impairments in clinical populations. Where applicable, examples of research conducted by the author and his colleagues will be used to illustrate these topics.

Humans are infected by 2 genetic assemblages (A and B) of Giardia duodenalis, a protozoan parasite that causes gastro-intestinal disease. Sub-assemblages AI, AII, BIII and BIV are commonly identified in human cases. Detection requires amplification of G. duodenalis loci. Subsequent DNA sequencing or restriction fragment length polymorphism (RFLP) identifies sub-assemblages but is expensive (DNA sequencing) or insensitive (RFLP). This study investigated a fluorescence-based detection method, using terminal-restriction fragment length polymorphism (T-RFLP) of the glutamate dehydrogenase gene to characterize human infections. Clinical samples (n=73), positive for Giardia were collected in New South Wales, Australia, and were used to evaluate T-RFLP detection. The accuracy and sensitivity of T-RFLP detection was established by comparison to DNA sequencing and RFLP. Sub-assemblage assignment by T-RFLP identified BIV as the common subtype in N.S.W cases, whilst AI, AII and BIII were also detected. When compared to DNA sequencing and RFLP, analysis by T-RFLP was a reliable and reproducible method. Automated fluorescent detection enabled accurate sizing of restriction fragments and provided a sensitive alternative to RFLP. Discrimination of sub-assemblages by T-RFLP was comparable to DNA sequencing, but was efficient and inexpensive. The protocol described here provides a rapid and sensitive diagnostic tool for routine sample screenings in epidemiological research.