Germplasm lines with resistance to the sugarbeet root maggot (SBRM) have been developed and released to the public, providing a means to generate hybrids with resistance against the most devastating insect pest of sugarbeet in North America. Effective use of this germplasm, however, requires knowledge of relative strengths of SBRM resistance between lines and knowledge of the diversity and genetic relationships between germplasm. Therefore, field studies comparing SBRM resistance of four released SBRM-resistant germplasm lines (F1015, F1016, F1024 and F1043), a SBRM-resistant parent (PI 179180) and an unreleased SBRM-resistant population (F1055) were performed, and genetic analysis of the diversity and relationships between SBRM-resistant germplasm and their available parents was conducted using simple sequence repeat (SSR) markers. Under natural SBRM infestations, resistant germplasm exhibited significantly less SBRM damage than a susceptible control, with similar, high levels of resistance in F1016, F1024, F1043, F1055 and PI 179180 and lower resistance in F1015. SSR analysis revealed genetic similarities between F1016, F1024 and F1055, while F1015 and F1043 were genetically distinct from these lines. Among resistant genotypes, F1015 and F1043 exhibited greatest and least within-line genetic diversity, indicating greater and lesser inbreeding for F1043 and F1015, respectively. Similarities in damage ratings and genetics for F1016, F1024 and F1055 indicate that these lines are likely to be equally effective at introducing SBRM resistance into elite populations and in combining ability. In contrast, F1043, with its unique parentage and genetic dissimilarity from other resistant lines, provides a genetically distinct, but similarly effective, source of SBRM resistance.

A standard weed management system (system I) had a higher return above variable costs than did an intensive weed management system (system II) for two eastern Colorado cropping rotations. For continuous corn (Zea mays L.), the return above variable costs averaged $18.85/ha more under system I than under system II. For a barley (Hordeum vulgare L.)-corn-sugarbeet (Beta vulgaris L.) rotation, the return above variable costs averaged $20.48/ha more under System I than under System II. Based on alternative input (herbicide) and product prices, higher herbicide costs favored the standard weed management system, whereas higher crop prices favored the weed management system with the higher yields adjusted for quality. The probability that returns above variable costs differed between the two weed management systems depended upon the level of product prices and herbicide costs.

Alfalfa (Medicago sativa L.), beans (Phaseolus vulgaris L.), corn (Zea mays L.), potatoes (Solanum tuberosum L.), spring wheat (Triticum aestivum L.), and sugarbeets (Beta vulgaris L.) were severely injured and had reduced foliage dry weights when grown in soil containing 0.10 ppmw or more terbacil (3-tert-butyl-5-chloro-6-methyluracil). Activated carbon at 150 kg/ha prevented a significant loss in weight of beans and corn in soil containing 0.10 ppmw terbacil and fully protected potatoes in soil containing 0.19 ppmw terbacil. Carbon at 300 kg/ha fully protected sugarbeets and prevented a significant weight loss in alfalfa in soil containing 0.19 ppmw terbacil. Carbon at 600 kg/ha did not protect wheat from 0.19 ppmw terbacil.

The impact of two weed management systems on the weed seed reserves of the soil, on the yearly weed problem, and on barley (Hordeum vulgare L. ‘Steptoe’), corn (Zea mays L. ‘Pioneer 3709′), and sugarbeet (Beta vulgaris L. ‘Mono Hy D2′) production was assessed where these crops were grown in rotation for 6 consecutive years. Weeds were controlled in each crop with a moderate (system I) or intensive (system II) level of herbicides, plus conventional tillage. Weed seeds from seven annual genera were identified, with redroot pigweed (Amaranthus retroflexus L. ♯3 AMARE) and Chenopodium comprising 56 and 30%, respectively, of the initial 1377 million weed seeds/ha that were present in the upper 25 cm of the soil profile. After the sixth cropping year, the overall decline in the total number of weed seeds in soil was 96% in system I and 97% in system II. Over the 6-yr period, about 1.3 times more weeds escaped control in system I than in system II; and within a crop, the fewest number of weeds escaped annually in sugarbeets, and the most in barley. Yields of barley grain, corn silage, and recoverable sucrose were similar each year in the two weed management systems.

The effect of oxyethylene (OE) chain length of three homologous series of nonionic surfactants (allinol, nonoxynol, octoxynol) on glyphosate uptake was markedly affected by the leaf surface fine-structure of sugarbeet and kohlrabi. Adaxial leaf surfaces of sugarbeet were covered with a layer of amorphous wax, whereas the adaxial surface of kohlrabi leaves was covered with fine crystalline wax. Foliar uptake of glyphosate (1 mM glyphosate, 20 mM glycine, pH 3.2) averaged 4% for sugarbeet without surfactant, but droplets were not retained by kohlrabi leaves in the absence of a surfactant. Glyphosate absorption with octoxynol (9 to 10 OE units, 0.5 g L−1) was rapid initially (0 to 2 h) and leveled off about 2 h after application in both species. Absorption by sugarbeet decreased from 12 to 3% as OE content of octoxynol was increased from 5 to 30 OE units. In contrast, surfactants of intermediate OE content (octoxynol, 16 OE units) induced the greatest uptake (17%) on kohlrabi. Leaf wetting was markedly affected by surfactant and leaf surface. As OE content of octoxynol increased from 5 to 30 OE units, droplet/leaf interface areas of 1-μl droplets decreased from 4 to 3 mm2 on the adaxial leaf surface of sugarbeet and from 61 to 2 mm2 on kohlrabi. Concurrently, the rate of droplet evaporation (1 μl) decreased from 1.0 to 0.7 nl s−1 on sugarbeet and 4.2 to 0.5 nl s−1 on kohlrabi leaves. The effect of OE content on enhancement of glyphosate uptake and wetting characteristics of spray solutions was similar within species for different hydrophobic moieties but differed markedly between species.

Experiments were conducted in controlled environmental chambers to determine the influence of temperature on the phytotoxicity of seven soil-applied herbicides. Diclofop {2-[4-(2,4-dichlorophenoxy)phenoxy] propanoic acid} soil incorporated or surface applied, was more toxic to wild oat (Avena fatua L.) shoots at 10 and 17 C than at 24 C. Efficacy of diclofop was enhanced with soil incorporation. Diclofop toxicity to wild oat roots was not influenced by a change in temperature. EPTC (S-ethyl dipropylthiocarbamate) stimulated sugarbeet (Beta vulgaris L. ‘American Crystal Hybrid B’) shoot dry weight production at 10 C and caused dry weight reduction at 24 C. Atrazine [2-chloro-4-(ethylamino)-6-(isopropylamino)-s-triazine] toxicity to barley (Hordeum vulgare L. ‘Larker’) and alachlor [2-chloro-2′,6′-diethyl-N-(methoxymethyl)acetanilide] toxicity to oats (Avena sativa L. ‘Chief’) increased with increased temperature from 10 to 17 C. Temperatures within the range of 10 to 24 C did not affect trifluralin (α,α,α-trifluoro-2,6-dinitro-N,N-dipropyl-p-toluidine) toxicity to barley or BAY-5653 [N-(2-benzothiazolyl)-N-methylurea] or chloamben (3-amino-2,5-dichlorobenzoic acid) toxicity to oats.

Sugarbeet tolerance to and Canada thistle control from tank mixtures of clopyralid plus other postemergence herbicides and/or petroleum oil adjuvant applied at various times were determined. Herbicides were applied to 5 to 20 or 20 to 70-cm tall Canada thistle. Clopyralid at 0.14 and 0.21 kg ai ha-1 alone or with petroleum oil adjuvant did not injure sugarbeet. Tank mixing clopyralid with desmedipham/phenmedipham plus endothall injured sugarbeet 7 d after application in 2 of 3 yr but not when observed 14 d after application. Herbicides applied early postemergence in 1987 and late postemergence in 1988 controlled Canada thistle poorly due to rainfall 1 h after application and drought conditions, respectively. The addition of petroleum oil adjuvant enhanced control of Canada thistle by clopyralid at 0.14 kg ha-1, and control equaled that of clopyralid at 0.21 kg ha-1 alone. Addition of desmedipham/phenmedipham plus endothall to clopyralid did not reduce Canada thistle control. Petroleum oil adjuvant added to clopyralid at 0.14 kg ha-1 increased Canada thistle control to that achieved by clopyralid at 0.21 kg ha-1 plus desmedipham/phenmedipham plus endothall.

The methyl ester of diclofop [2-(4-(2,4-dichlorophenoxy) phenoxy) propionic acid] was used in all of a series of experiments. When it was applied postemergence, it selectively controlled barnyardgrass [Echinochloa crus-galli (L.) Beauv.] in sugarbeets (Beta vulgaris L. ‘USH 9′). Tank-mixing diclofop with desmedipham [ethyl m-hydroxycarbanilate carbanilate (ester)] resulted in decreased control of barnyardgrass under greenhouse and field conditions. Losses in activity ranged from 10 to 80%. Delaying application of desmedipham following application of diclofop by 4 days in the greenhouse or in the field resulted in no reduction in barnyardgrass control. Increased rates of diclofop offset the antagonistic effect of desmedipham. Similar losses in activity occurred when Hoe-29152 {methyl-2-[4-(4-trifluoromethylphenoxy)phenoxy] propanoate} was tank-mixed with desmedipham.

Field and greenhouse experiments were established to determine the phytotoxicity of Canada thistle [Cirsium arvense (L.) Scop.] residue on crop growth. Field examination of infestations showed that as Canada thistle shoots increased in an area, the number of kochia [Kochia scoparia (L.) Schrad.], marshelder (Iva xanthifolia Nutt.) and foxtail barley (Hordeum jubatum L.) plants decreased. Conversely, as Canada thistle shoots decreased, the aforementioned annual and perennial plants increased. In greenhouse studies, roots and shoots of Canada thistle that were mixed with soil reduced the growth of sugarbeet (Beta vulgaris L. ‘Mono Hy D2’), wheat (Triticum aestivum L. ‘Centurk’), alfalfa (Medicago sativa L. ‘Dawson’), and Canada thistle seedlings. Corn (Zea mays L. ‘Jacques No. 1004’) and dry edible beans (Phaseolus vulgaris L. ‘Great Northern No. 59’) grown in soil treated with Canada thistle residue were affected to a lesser extent. When Canada thistle residue was mixed with soil, crop growth was inversely proportional to the amount of Canada thistle residue added to the soil. Both roots and shoots of Canada thistle were toxic to crops when mixed with the soil. The effects of Canada thistle residue on crop growth lasted for about 60 days. Neither autoclaving residue and soil nor fertilization of soil had any effect on residue toxicity. Canada thistle leaf leachate inhibited the growth of sugarbeets watered daily with the leachate.

Loss in activity of foliar-applied methyl ester of diclofop {2-[4-(2,4-dichlorophenoxy)phenoxy] propanoic acid} occurred under low soil moisture conditions. A loss in control of yellow foxtail [Setaria lutescens (Weigel) Hubb.], wild oats (Avena fatua L.), little-seed canarygrass (Phalaris minor Retz.), and barnyardgrass [Echinochloa crus-galli (L.) Beauv.], was observed under greenhouse and growth chamber conditions. When soil was maintained at 2 to 3% above wilting point as compared to near field capacity, herbicide activity was decreased by 15 to 50%. High soil moisture (at or above 67% of field capacity) for at least 2 to 4 days following treatment was needed to achieve maximum effectiveness of the herbicide. Daily furrow irrigations for a period of 10 days following treatment of barnyardgrass in the field resulted in highest activity as compared to that under single irrigation regimes within the 10-day period. The effect of low soil moisture was minimized by increased rates of herbicide application. Hoe-29152 {methyl-2-[4-(4-trifluoromethylphenoxy)phenoxy] propanoate} showed similar losses in activity associated with low soil moisture. No consistent changes in uptake or translocation of 14C-labeled diclofop could be detected in association with altered soil moisture status.

Phenmedipham metabolism in leaf tissue of sugarbeet (tolerant) and rapeseed (sensitive) was compared. Sugarbeet leaf discs metabolized phenmedipham much more rapidly than rapeseed leaf discs, forming two metabolites of relatively low polarity. The less polar of these (metabolite 21) was a precursor to the other (metabolite 11), and its properties indicate derivation from phenmedipham by a single hydroxylation and monoglycosylation. Synthetic N-hydroxyphenmedipham was converted by both species into a compound that cochromatographs with metabolite 21. Purified metabolite 21 was much less inhibitory to light-driven oxygen evolution by isolated thylakoids of both species than was phenmedipham. Hydroxylation/glycosylation without prior carbamate hydrolysis appears to be a major factor in the tolerance of sugarbeet to phenmedipham.

In the field, eight barley (Hordeum vulgare L.) cultivars tolerated postemergence applications of AC 222,293 [a mixture of methyl 6 and 2-(4-isopropyl-4-methyl-5-oxo-2-imidazolin-2-yl) m- and p-toluate] at 0.4 to 1.4 kg/ha. AC 222,293 at 0.4 kg ai/ha applied at the 1.5- to 2-leaf stage controlled wild oats (Avena fatua L. # AVEFA) 96%, whereas 0.7 kg/ha applied at the 3.5- to 4-leaf stage controlled wild oats only 93%. Sugarbeets (Beta vulgaris L.) but not alfalfa (Medicago sativa L.), corn (Zea mays L.), pinto beans (Phaseolus vulgaris L.), or sunflower (Helianthus annus L.), were injured when planted 6 to 8 months after fall applications of AC 222,293 at 0.4 to 1.1 kg/ha.

Five sulfonylurea herbicides were applied to Typic Boroll soil with pH 8.0 and 2% organic matter in the field and growth chamber to assess their residual effects on following crops. Under irrigated conditions, triasulfuron at 22 g/ha reduced the growth of alfalfa, canola, corn, lentil, pea, potato, and sugarbeet the year after application but caused no injury after two years. Alfalfa GR50 (rate required to reduce growth by 50%) values immediately after application of chlorsulfuron, triasulfuron, and metsulfuron were < 2.2 g/ha and similar in the growth chamber and field. Alfalfa and canola GR50 values immediately after thifensulfuron application in the growth chamber were ≥ 90 g/ha. Alfalfa, canola, flax, and sugarbeet GR50 for tribenuron in the growth chamber were < 4 g/ha. Crop response curves developed by growth chamber experiments could replace many of the multiple site and crop field experiments now required to develop recropping guidelines for residual herbicides.

An economic analysis of four weed management systems employed on four crop sequences in a barley-corn-pinto bean-sugarbeet rotation in eastern Colorado was computed. Weeds were controlled in each crop with only conventional tillage or conventional tillage plus minimum levels of herbicides (systems 3 and 4), moderate levels of herbicides (system 1), or intensive levels of herbicides (system 2). Adjusted gross returns were higher for systems 3 and 4 where herbicide use was less/year and decreased over 4 yr than for systems 1 and 2 where herbicide use was higher/year and constant. When the four crop sequences were aggregated using yield and sucrose indices, the least herbicide-intensive weed management system had $440/ha/4 yr higher indexed adjusted gross return than the most herbicide-intensive weed management system. An income risk analysis showed that the herbicide-intensive weed management system was not risk efficient and that producers would select one of the other three less herbicide-intensive weed management systems depending upon their risk preferences.

The applicability of fluorescence measurements for the detection of herbicide effects in whole leaves was analyzed. Based on the results known for isolated chloroplasts, normalized variable fluorescence of the initial rise was shown to be an appropriate tool for monitoring effects of photosystem II (PS II) herbicides. Equipment is described for monitoring the degree of inhibition by fluorescence induction measurements and microcomputer data analysis. The method is used to study the effect of pyrazon [5-amino-4-chloro-2-phenyl-3(2H)-pyridazinone], BAY DRW 1139 [4-amino-3-methyl-6-phenyl-1,2,4-triazin-5(4H)-one], and phenmedipham {3-[(methoxycarbonyl)amino] phenyl (3-methyl-phenyl)carbamate} after foliar application to different species. A rapid decrease of normalized variable fluorescence indicates penetration into leaf cells of all species tested. During a 5- to 7-day experiment, the apparent variable fluorescence decreased continuously in herbicide-susceptible plants, while it recovered in resistant plants due to an internal detoxification mechanism. The described method provides a rapid, simple, and nondestructive tool for analyzing the kinetics of penetration and detoxification of PS II herbicides in whole leaves.

The impact of four weed management systems on weed seed reserves in soil, yearly weed problem, and production of barley, corn, pinto bean, and sugarbeet was assessed where these crops were grown in rotation for 4 consecutive years in four cropping sequences. Weeds were controlled in each crop with only conventional tillage or conventional tillage plus minimum, moderate (system 1), and intensive (system 2) levels of herbicides. Seed of annual weeds from 11 genera were identified, with barnyardgrass and redroot pigweed comprising 66 and 19%, respectively, of the initial 90 million weed seed/ha present in the upper 25 cm of the soil profile. After the fourth cropping year, overall decline in total number of weed seed in soil was 53% when averaged over four cropping sequences and four weed management systems. Over the 4-yr period, about 10 times more weeds escaped control in system 1 than in system 2; and within a crop, the fewest number of weeds escaped control annually in barley. System 2 had the highest herbicide use in each cropping sequence, the fewest weeds at harvest, and the smallest adjusted gross return over the 4-yr period in three of four cropping sequences.

An integrated approach based on the use of bioinformatics and gene expression analysis tools was carried out to evaluate the organ-specific transcription modulation of nine genes relevant to sugar metabolism of Beta vulgaris L. plantlets of the autumn cv. Franca and spring cv. Bianca, in response to low-temperature (LT) treatments. Different growth cycles imply different plant capability to adapt to the environment that includes variations in gene expression of key metabolic enzymes. The transcriptional response was evaluated by quantitative PCR analysis before, during and after the LT treatments. The results were correlated with the LT-induced electrolyte leakage measure and the carbohydrate content. Stress-induced transcript level alterations were detected in the two cultivars, suggesting a modulation of sucrose synthesis and carbohydrate partitioning. Cold stress induced deep changes in the autumn cultivar, especially in fructose-1,6-biphosphatase gene expression, irrespective of temperature or exposure time. These differential features of expression profiles constitute first clues on the molecular basis of the differential LT response of sugarbeet autumn and spring cultivars.

The seed reserves of sugar beet (Beta vulgaris L.) are starch, located exclusively in the perisperm, and lipids, proteins and a small quantity of soluble sugars, located mainly in the cotyledons. It is shown that lipids are the main respiratory substrate used during germination whereas starch and remaining lipids are only hydrolysed after root extrusion, to sustain root and hypocotyl growth. By removing the perisperm during imbibition, it was demonstrated that lipids and proteins alone are sufficient for viable seedling development, though such seedlings grew less than those developing in the presence of perisperm. The utilization of seed reserves was followed during seedling development in the dark in various organs. At 20°C, the reserves were sufficient for 8 d of growth in the dark, the hypocotyl attaining a length of 5 cm. Specific problems relating to field establishment of sugar beet are discussed in relation to these results.

Sugarbeet (Beta vulgaris) seeds which had undergone an advancement treatment germinated more quickly and showed greater germination in a standard test at 9°C than seeds which had been steeped in water. Both treatments included the fungicide, thiram (tetramethyl thiuram disulphide). Advanced seeds also emerged more quickly and showed higher establishment in the field. The levels of extractable high molecular weight (HMW) RNA and DNA from advanced, thiram-steeped and untreated seeds were compared. Advanced seeds contained significantly (P > 0.05) more HMW RNA and DNA than thiram-steeped or untreated seeds. Extractable DNA levels were higher from thiram-steeped seeds than untreated seeds but not significantly so. Cell-cycle studies on advanced seeds demonstrated an increase from 2% to 24% of root-tip nuclei with DNA contents of 4C and above. This indicates that DNA replication occurred during the advancement treatment. There was no evidence of cell division occurring, indicating that: (1) advanced seeds were arrested post-S-phase and (2) the period following DNA synthesis in sugarbeet seeds is desiccation tolerant.

Concerns have been raised in Europe about the efficiency, sustainability, and environmental impact of the first genetically modified crops. The committees and regulators in charge of approving procedures have encouraged a field trial approach for safety assessment studies under current agronomic conditions. We describe the gene flow from sugar beet (Beta vulgaris L.) in a multi-year and multi-crop monitoring study on farmers' fields at two locations that has been carried out since 1995. We analyzed two sugar beet lines that have been genetically transformed for herbicide resistance. One sugar beet has resistance to glufosinate and the other to glyphosate. Large differences among lines, years and locations were observed. These differences provided a broad range of situations to estimate the risks. Sugar beet bolters produced the majority (86%) of the herbicide-resistant seeds harvested in the field. Direct pollen flow from sugar beet bolters to weed beets that were growing within the same field as well as in a neighboring field that was left fallow accounted for only 0.4% of the resistant seeds released over the years and locations. Descendants of the hybrids between the sugar beet and the weed beet produced the remaining 13.6% of resistant seeds. Herbicide-resistant seeds from the progeny of the weed beet were recorded up to 112 m away from the closest transgenic pollen donor. Indications were observed of non-randomness of the weed beet producing resistant progeny. We also analyzed pollen flow to male-sterile bait plants located within and outside of the sugar beet field. Herbicide-resistant pollen flow was recorded up to 277 m, and fitted with an inverse power regression. Using sugar beet varieties with no, or very low, sensitivity to bolting and destroying bolters are two necessary measures that could delay gene flow.